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Öğe Akkaraman Irkı Koyunlarda Cervix Uteri'ni?n Yüzey ve Kri?pt Epi?teli? Üzeri?nde Hi?stolojik ve Hi?stoki?myasal Çalışmalar(Selçuk Üniversitesi, 1994) Eren, Ülker; Aksoy, Melih; Aştı, Reşat N.; Çınar, Kenan; Güven, BülentThe aim of this study was to investigate the histologic and histochemical characteristics of cervical epithelium of Akkaraman sheep. As a material. 22 cervices of ewes were used, which 10 of them were in folicular phase and 12 of them were lut e al phase. lt was determined that Lamina epithelialis was simple columnar type and composed of secretory and ciliated eel/s. The epithelium was replaced by stratified vaginal epithelium in the caudal cervix. Any glandufar structure was not observed except in seven cases. In these cases, besides the glanduler structures hyperplasic focuses and squamous metaplasia were found. lt was esrimared that this findings appeared due to phytooestrogens or elements which have estrogenic effects. By means of histochemical methods, the cervical seeret was found to be a mixture of acidic and neutral mucosubstances. The secretory material contained both neutral and acidic mucosubstance in lateral and apical parts of villi. But in crypts, carboxylated 'acidic mucosubstance was dominant. lt was determined that carboxylated acidie mucosubstance ineressed especially in crypts in the follicular phase. Als o it w as shown that, acidic mucosubstance was dominant in the secretory material of glands.Öğe Cholesterol-Loaded Cyclodextrin Enhances Osmotic Tolerance and Inhibits the Acrosome Reaction in Rabbit Spermatozoa(Elsevier Science Bv, 2010) Aksoy, Melih; Akman, Orhan; Lehimcioğlu, Necdet Cankat; Erdem, HüseyinThe effects of cholesterol-loaded cyclodextrin (CLC) treatment on the osmotic tolerance and ability to undergo the acrosome reaction of rabbit spermatozoa, with an unusually high cholesterol/phospholipid ratio in plasma membranes, were examined in two successive experiments. In the first experiment, CLC-pretreated and untreated sperm cells were exposed for 15 min to one of five fructose solutions, adjusted to 20, 80, 290, 500 or 1500 mOsm/L. After the anisoosmotic challenge, the integrity of sperm membranes in the CLC-supplemented (at a dose level of 3 mg/120 x 10(6) spermatozoa) and control groups was estimated by a modified hypoosmotic swelling test (HOST) associated with a supravital eosin staining test (HE-test). In the second part of the study, the influence of cholesterol supplementation on the acrosome reaction of sperm cells stimulated by either calcium ionophore A23187 (CI) or lysophosphatidylcholine (LPC) was evaluated. CLC pretreatment increased viable and live-HOST-responsive sperm rates (P<0.01) after incubation in anisoosmotic solutions varying from 80 to 1500 mOsm/L However, CLC supplementation did not influence the percentage of HOST-responsive sperm cells (P>0.05). A significant interaction was determined between CLC pretreatment and the level of osmotic pressure in maintaining the functional and physical integrities of sperm membranes undergoing osmotic challenges. Both CI and LPC successfully induced the acrosome reaction in rabbit spermatozoa (P<0.001). Compared with CI, LPC was more effective (P<0.0001). CLC pretreatment resulted in a significant reduction (P<0.01) in the percentage of acrosome reacted sperm cells irrespective of the inducing agent, either CI or LPC. In conclusion, CLC treatment enhanced the anisoosmotic tolerance of rabbit spermatozoa and reduced their ability to undergo the acrosome reaction after stimulation by CI or LPC.Öğe Düvelerde Deği?şi?k Yollardan ve Farklı Dozda Veri?len Luprosti?ol'ün Luteoli?ti?k Etki?si? ve Gebeli?k Oranının Araştırılması(Selçuk Üniversitesi, 1993) Güler, Mehmet; Dinç, D. Ali; Semacan, Ahmet; Aksoy, Melih; Işık, Kürşat; Erdem, HüseyinBu çalışmada farklı yollardan düşük dozda PGF2a uygulanarak luteolitik etkisi ve bunu takiben elde edilen gebelik oranı araştırıldı Tubüler genital organlarında herhangi bir bozukluk olmayan ve ovaryumları aktivite gösteren 2-3 yaşında 15 baş İsviçre Esmeri düve, senkronize edildikten sonra (15 mg Luprostiol IM) rastgele 3 gruba ayrılarak, 1. gruba (IM) bir prostaglandin Fu analoğu olan Luprostiol 15 mg Intramüsküler, 2 gruba (IVSM) 3.75 mg intravulva-submukozal ve 3. gruba (IO) 0 75 mg intraovarian yoldan enjekte edildi. Enjeksiyondan hemen önce ve bunu takibeden 5 gün süreyle jugular kan örnekleri toplanarak plazma progesteron miktarı belirlendi Östrüsün diş belirtilerinin gözlenmesi ve plazma progesteron miktarı belirlenerek yapılan değerlendirme sonucuna göre, her üç gruptan birer hayvanda luteolizis oluşmadı. IM ve 10 prostaglandin uygulanan grupta geriye kalan hayvanların tümünde (4 hayvan) luteolizis oluştu, IVSM prostaglandin verilen grupta 4 hayvandan 2'sinde (@) luteolizis oluşurken kalan 2'sinde (@) luteolizis'te gecikme gözlendi. Plazma progesteron miktarı 1. ve 3. gruptaki hayvanlarda ilaç uygulamasından sonraki 24 saatte 1 ng/mi'nin altına düşerken 2 grupta bunlara nazaran daha yüksek se- viyede kaldı. Östrüsün diş belirtilerine dayanılarak yapılan tesbitte, IM ve IVSM gruplarda östrüslerin 49-72 saatler arasına yoğunlaştığı gözlenmesine karşılık, lo grupda diğer iki gruba nazaran daha erken dönemde oluştu (24-36 saatler arasında). Östrüs belirtilerine göre bu sürenin ikinci yarısında sun'i tohumlama uygulanan hayvanlarda gebelik oranı ise 10 grupta en yüksek olarak bulundu (%100)Öğe Düvelerde suni tohumlama sırasında farklı yollardan uygulanan buserelin'in uvulasyon zamanı ve fertilite üzerine etkisi(1998) Ataman, M.Bozkurt; Aksoy, Melih; Kaya, Abdullah; Aral, Faruk; Yıldız, Cengiz; Aköz, MehmetBu çalışmada, düvelerde tohumlama anında farklı " yollardan uygulanan buserelin'in ovulasyon zamanı ve gebe kalma oranlan üzerine etkisi araştırıldı. Materyal olarak 24 baş düve kullanıldı. Düveler üç gruba ayrılarak tohumlama anında birinci gruba (n8) 0.0105 mg (2.5 mi) buserelin intramuskuler olarak enjekte edildi. İkinci gruba (n8) 0.1 mg buserelin acetat burun mukozasına püskürtüldü. Üçüncü gruba (n8) ise 1 mi steril serum fizyolojik intramuskuler olarak enjekte edildi. Ovulasyon zamanları altışar saat aralıklarla uygulanan rektal palpasyonla tespit edildi. Bir, iki ve üçüncü grupta ikinci PGF_{2\alpha} enjeksiyonu-ovulasyon intervali sırasıyla 68.92.79, 70.62.03 ve 78.11.10 saat olarak tespit edildi. Gebe hayvanlar tohumlamayı takiben 19. günde kan-progesteron düzeyleri yardımıyla belirlendi. Gebejik oranları bir, iki ve üçüncü grupta sırasıyla %87.5, %87.5, %62.5 olarak tespit edildi. Sonuç olarak, tohumlama anında gerek intramuskuler gerekse nasal yolla GnRH uygulamalarının ikinci PGF_{2\alpha} enjeksiyonu-ovulasyon intervalini kısalttığı ve gebelik oranlarını arttırdığı kanısına varıldı.Öğe Effect of Melatonin Implantation to Sperm Donor Rams on Post-Thaw Viability and Acrosomal Integrity of Sperm Cells in the Breeding and Non-Breeding Season(BLACKWELL WISSENSCHAFTS-VERLAG GMBH, 2001) Kaya, Abdullah; Aksoy, Melih; Başpınar, Nuri; Yıldız, Cengiz; Ataman, Mehmet BozkurtThe influence of melatonin administration to sperm donors on the freezability of ram semen and enzyme leakage through sperm cells during different steps of the cryopreservation process were evaluated in the breeding and non-breeding season. Melatonin implantation to rams in the breeding season improved post-thaw sperm viability and intact acrosome rates without influencing the motility rate (p < 0.05). Likewise, the post-thaw alkaline phosphatase release through sperm cells was significantly lower in the melatonin-treated group in comparison with untreated controls (p < 0.05). In the non-breeding season, melatonin administration enhanced intact acrosome rates (p < 0.05) and reduced aspartate aminotransferase activity (p < 0.05) postthaw in the offseason ejaculates. Melatonin implantation twice in the breeding and non-breeding season did not produce any further improvement in the post-thaw sperm parameters in the non-breeding season ejaculates. It was concluded that melatonin administration to sperm donors improved freezability of ram semen collected from these rams and reduced enzyme leakage through sperm cells during cryopreservation.Öğe Effect of Seasonal Conditions on Oestrus Occurrence and Postpartum Period in Anatolian Water Buffaloes(M H Schaper Gmbh CO Kg, 2002) Aksoy, Melih; Kaya, Abdullah; Uçar, Mehmet; Lehimcioğlu, Necdet Cankat; Tekeli, TevfikOestrus and calving records of Anatolian buffaloes were analyzed to investigate influence of climatic conditions on oestrus occurrence and postpartum period. Oestrus records showed a seasonal pattern concentrated between July and September which is the warmest period of the year. Likewise, more than half (52.7%) of the total calvings occurred between May and August. Month of calving significantly influenced the length of calving to first oestrus interval and open period (P < 0.01). The average length of open period was significantly shorter in autumn and summer calvers compared to those calved in spring and winter (P < 0.05) during long days. The humidity rate was negatively correlated with both calving to first oestrus interval and open period (P < 0.05). In conclusion, exposure to high environmental temperatures around 20 degreesC exerted no suppressive influence on ovarian activity in Anatolian buffaloes. Although humidity rate (P < 0.05) and ambient temperature (P < 0.01) was found to be correlated with the duration of postpartum period, day length might be the main factor regulating reproductive biorhythm and postpartum events in Anatolian buffalo cows.Öğe Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4 degrees C or freezing(UNIV POLITECNICA VALENCIA, 2008) Aksoy, Melih; Lehimcioğlu, Necdet Cankat; Akman, OrhanThe effect of semen plasma removal either by simple centrifugation or by separation through a Percoll gradient on the integrity of plasma membranes of rabbit spermatozoa during storage at 4 degrees C and freezing was evaluated in two successive experiments. A modified hypo-osmotic swelling test procedure combined with supravital staining was employed to evaluate simultaneously membrane integrity of head and tail membranes of sperm cells. In the first experiment, the impact of semen plasma on membrane integrity of sperm cells was examined in Tris-citric acid-glucose extender at 4 degrees C for 96 h. The percentage of sperm cells with disintegrated tail and head membranes increased in all groups in correlation with the length of storage. After storage for 96 h, removal of semen plasma, irrespective of the method of removal, resulted in significant increase (P<0.01) in the percentage of sperm cells with disintegrated plasma membranes. The adverse effect of storage and removal of semen plasma was more prominent on the tail membranes, and especially during the first 24 h of the storage period. In the second experiment, the impact of semen plasma on membrane integrity of sperm cells undergoing freezing was examined. A total of three groups were arranged as described in the first experiment, and semen samples were frozen in straws using an extender including acetamide and methyl cellulose. Freezing of semen drastically reduced the percentage of sperm cells with intact plasma membranes in post-thaw samples. However, removal of semen plasma, irrespective of the method of removal, did not affect the proportion of sperm cells with intact plasma membranes. In conclusion, the effect of semen plasma on plasma membranes varied significantly relative to the preservation temperature of sperm cells. Although it exerted a protective influence during storage at 4 degrees C, no protective impact was monitored during freezing.Öğe Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4°C or freezing(2008) Aksoy, Melih; Lehimcioğlu, Necdet Cankat; Akman, OrhanThe effect of semen plasma removal either by simple centrifugation or by separation through a Percoll gradient on the integrity of plasma membranes of rabbit spermatozoa during storage at 4°C and freezing was evaluated in two successive experiments. A modified hypo-osmotic swelling test procedure combined with supravital staining was employed to evaluate simultaneously membrane integrity of head and tail membranes of sperm cells. In the first experiment, the impact of semen plasma on membrane integrity of sperm cells was examined in Tris-citric acid-glucose extender at 4°C for 96 h. The percentage of sperm cells with disintegrated tail and head membranes increased in all groups in correlation with the length of storage. After storage for 96 h, removal of semen plasma, irrespective of the method of removal, resulted in significant increase (P<0.01) in the percentage of sperm cells with disintegrated plasma membranes. The adverse effect of storage and removal of semen plasma was more prominent on the tail membranes, and especially during the first 24 h of the storage period. In the second experiment, the impact of semen plasma on membrane integrity of sperm cells undergoing freezing was examined. A total of three groups were arranged as described in the first experiment, and semen samples were frozen in straws using an extender including acetamide and methyl cellulose. Freezing of semen drastically reduced the percentage of sperm cells with intact plasma membranes in post-thaw samples. However, removal of semen plasma, irrespective of the method of removal, did not affect the proportion of sperm cells with intact plasma membranes. In conclusion, the effect of semen plasma on plasma membranes varied significantly relative to the preservation temperature of sperm cells. Although it exerted a protective influence during storage at 4°C, no protective impact was monitored during freezing. © WRSA, UPV, 2003.Öğe Effect of site of insemination on bovine fertility(1997) Ataman, Mehmet B.; Taşal, İbrahim; Aksoy, Melih; Karaca, Fikret; Kaya, Abdullah; Tekeli, TevfikBu çalışmada, suni tohumlama yapılan inek ve düvelerde sperma bırakılma yerinin gebelik oranları üzerine etkisi araştırıldı. Çalışmada 82 baş inek ve 18 baş düve olmak üzere toplam 100 baş hayvan cervical, corpus uteri, Graaf folliküiünün iokalizasyonuna göre ipsilateral ve kontralateral cornu tohumlama bölgesi olmak üzere 4 eşit gruba ayrılarak tohumlandılar. Gebelik oranları gruplarda sırasıyla %88, %72, %84 ve %72 olarak tespit edildi. Sonuç olarak, spermanın corpus uteri veya ipsilateral cornuya depo edilmesinin gebelik oranını arttırdığı, cervical yolla tohumlamanın herhangi bir avantajı olmadığı kanısına varıldı.Öğe Estimation of early pregnancy by electrical resistance values of vaginal mucosa in cows and heifers(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2005) Taşal, İbrahim; Ataman, Mehmet Bozkurt; Aksoy, Melih; Kaya, Abdullah; Karaca, F; Tekeli, TevfikThe aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable reproductive disorders were used. Cloprostenol, a synthetic analogue of PGF(2 alpha), was administered intramuscularly twice (500 mu g) 11-days apart. After second cloprostenol administration, animals were observed for the overt signs oestrus and, inseminated twice with frozen semen after confirmation of oestrus by rectal palpation. Electrical conductivity of vaginal mucosa was measured through ventral wall of vagina adjacent to caudal end of cervix uteri by a handy-held instrument. Vaginal conductance of animals was measured twice, with a 12 h interval, on days 0 (day of oestrus), 3, 6, 10, 11 19 20 and 21 in cows, and on days 0 (day of oestrus), 3, 6, 10, 13, 18, 19 and 20 in heifers. To estimate plasma progesterone levels, jugular blood samples (10 mL) were also collected on days 19, 20 and 21 for cows, and 18, 19 and 20 for heifers. Pregnant animals were detected by rectal palpation 60 days after insemination. In pregnant cows, electrical resistance of vaginal mucosa measured between days 19-21 was significantly higher than non-pregnant animals, while vaginal resistance values determined on the day of insemination, and on days 3, 6, 10 and 13 post-insemination were similar in both groups. Likewise, vaginal impedance readings in pregnant heifers determined between days 18-20 were significantly higher than those non-pregnant animals. Vaginal resistance measurements in cows (between days 19-21) and heifers (between days 18-20) were significantly correlated with plasma progesterone concentrations both in pregnant and non-pregnant groups. It was concluded that vaginal resistance values might be used for the diagnosis of early pregnancy as a contributor or predictor method in cows and heifers.Öğe Estrus and Pregnancy Rates of Konya Merino Ewes Treated with a Double Injection of Cloprostenol at Different Intervals(ARCHIV FUR TIERZUCHT, 1997) Tekeli, Tevfik; Aksoy, Melih; Semacan, A.; Karaca, F.; Ayar, A.The effect of a double injection of cloprostenol given at different intervals on estrus and pregnancy rates after artificial insemination were evaluated in cycling Konya Merino ewes. Two injections of 125 mu g of cloprostenol were given to the ewes in group I (n=30), II (n=30) and III (n=31) at an interval of 8, 11 or 14 days, respectively. Ewes in group IV (n=28) served as control and received a double injection of saline at an interval of 8 days. Estrus ewes were detected by teaser rams during five days, 17 days in group IV, following the second treatment and intracervically inseminated by fresh semen. Estrus and pregnancy rates after first insemination in group I II, III and IV were 86.7, 100.0, 83.9 and 89.3% and 50.0, 60.0, 80.6 and 75.0%, respectively. It was concluded that an interval of 14 days may provide better pregnancy rates compared to those 8 and II days intervals in artificially inseminated ewes.Öğe Fare Ovumlarının In Vitro Fertilizasyonu Üzeri?nde Çalışmalar(Selçuk Üniversitesi, 1990 Ocak) Tekeli, Tevfik; Güler, Mehmet; Aksoy, MelihThe aim of this study was to test the recommended techniques of in vitro fertilization in mice as an experimental animal. Adult female mice of Swiss albino strain were superovulated by intraperitonal Injection of PMSG and HCG 48 hours apart. They were killed 16 hours after injection of HCG and their eggs were recovered under warm oil in a plastic dish containing 0.4 ml of incubation medium. Sperms recovered from cauda epididymis of adult males of the same strain were suspended in a medium and incubated at 37 deg C under % 5 CO? in air for 1.5-2 hours before they were used for insemination. The incubation medium was THY me- dium containing glucose, sodium pyruvate, bovine albumin and antibiotics. At the time of insemination, a drop of sperm suspension (10-20 ?l) was added to the medium containing egg clot. Eggs and sperms were incubated in incubation medium for 6 hours. In the first group, fertilized eggs were left into the same medium. In the second group they were transferred to the development medium (Modified Whitten's) without sodium lactate and incubated until they were examined under stereomicroscope. As a conclusion, in this study fertilized eggs and 2-cell stage embryos were obtained but further stage of embryos (morulae, blastocyst) couldn't be reached.Öğe GnRH Response Test and Libido Scores in Normal and Low-Quality Sperm Producing Rams(BLACKWELL WISSENSCHAFTS-VERLAG GMBH, 1993) Aksoy, Melih; Tekeli, Tevfik; Çoyan, K.; Güven, B.; Özar, S.; Alan, M.; Ayar, AliTesticular tissues are responsible for the androgen synthesis as well as the spermatogenesis. Production rate of androgens is affected by many factors. In addition to the hypothalamo- pituitary control, possibly a local substance, which is released by damaged seminiferous tubules, also plays an important role in androgen production of the Leydig cells (Aoki & Fawcett, 1978). Testosterone synthesis can be stimulated by the injection of exogenous gonadotropins and releasing hormones (Hocherau et al., 1990; Post et al., 1987 a,b). Lunstra & Schanbacher (1988) showed that cryptorchid rams had lower basal testosterone levels and a lesser ability to secrete testosterone in response to exogenous LH. Byers & Glover (1984) investigated the effect of scrotal insulation on hormone levels and pointed out that the mean and basal testosterone levels were significantly reduced after 14 days following scrotal insulation. Veeramachaneni et al. (1986) suggested that azoospermic bulls have lower baseline testosterone levels and testosterone response rates to GnRH injections than nor- mozoospermic bulls. GnRH induced testosterone levels in stallions are used as a diagnostic tool to differentiate cryptorchid and castrated animals (Nachrainer, 1986). Post et al. (1987b) reported that GnRH induced testosterone levels might give a valuable information on the selection of the bulls which are adequate to reproductive potential. The present study was designed to evaluate the GnRH response test results of low quality sperm producing and normozoospermic rams comparatively. Libido tests were also performed to assess the libido level of rams with normal or low sperm output.Öğe Holstein ırkı ithal gebe düvelerden oluşan sürünün doğum sonrası dölverimi performansının değerlendirilmesi(1998) Tekeli, Tevfik; Erdem, Hüseyin; Uçar, Mehmet; Aksoy, Melih; Yenice, MustafaSunulan çalışmada, Konya'da bulunan özel bir sütçü işletmeye ait, tamamı Türkiye'ye ithal edilen 270 baş damızlık gebe düveden oluşan bir sürünün doğumlarından sonra dölverimi performansı değerlendirildi. İthalat sonrası 10. ayda düvelerin yaptığı doğum şekli, suni tohumlamalarda elde edilen gebelik oranı ve infertilitenin etiyolojisi rektal palpasyon ve ultrasonografık muayenelerle belirlendi. Sürüde ithalat sonrası normal doğum, abortus, güç doğum, ölü doğumların oranları sırasıyla % 81.3, 6.0, 10.1, 2.6 olarak bulundu. Sürünün ithalat sonrası 10. ayda yapılan muayenesinde gebelik oranı % 57.8 olarak tespit edildi. Gebe olmadığı belirlenen ineklerin % 15.9'unda uterus enfeksiyonu, % 8.9'unda ovaryum kisti ve % 17.4'ünde de dölverimi ile ilgili diğer sorunlar belirlendi. Birinci, ikinci ve üçüncü tohumlamada gebelik oranları sırasıyla % 25.9, 50 ve 57 olarak tespit edildi. Gebe kalan ineklerde gebelik başına yapılan tohumlama sayısı 1.67 olarak bulundu. İlk üç tohumlamada gebe kalan bütün inekler ile birinci, ikinci ve üçüncü tohumlamalarda gebe kalan ineklerde doğum-ilk tohumlama aralığı sırasıyla 76.41.9, 79.63.1, 74.42.7, 71.74.6 gün olarak belirlendi. İkinci ve üçüncü tohumlamalarda gebe kalan ineklerde gözlenen toplam 103 östrüs siklusunun % 34.0'nün normal sayılan 18-24 günlük sınırlar içerisinde, % 66.0'nın bu sınırların dışında olduğu tespit edildi. Sonuç olarak, ithal edilen gebe düvelerde bakım ve idari sorunların yanı sıra Türkiye koşullarına adaptasyon güçlüğüne bağlı olarak dölverimi ile ilgili büyük sorunlarla karşılaşıldığı; bu nedenle düve veya inek ithalatı yerine en azından adaptasyon sorunlarının giderilebilmesi amacıyla donmuş embriyo ithalatının daha başarılı olacağı kanısına varıldı.Öğe I?neklerde Corpus Luteumun Morfolojik Özellikleri ve Endokrinolojik Aktivitesi Arasındaki İlişkinin Araştırılması(2000) Kaya, Abdullah; Erdem, Hüseyin; Aksoy, Melih; Tekeli, TevfikBu çalışmada luteal evredeki ineklerde corpus luteum (CL)' un morfolojik özellikleri ile endokrinolojik aktivitesi arasındaki ilişki belirlendi. Araştırma kesim amacıyla mezbahaya getirilen toplam 135 baş Holstein ırkı inek üzerinde gerçekleştirildi. İnekler önce rastlansal olarak numaralandırıldı ve kesimden önce kan örnekleri toplandı. Kesim sonrasında her ineğin ovaryum ve uterusları toplanarak laboratuvara getirildi. Gebe olan, inaktif ovaryumlu veya seksüel siklusun diöstrüs evresi dışında bulunan inekler çıkartıldıktan sonra gözlenebilir aktif CL' a sahip ve plazma progesteron düzeyi l ng/ml' den yüksek 60 baş inek araştırma grubuna alındı ve bulgular değerlendirildi.Corpus luteum çapı. ağırlığı ve plazma progesteron düzeyi arasında önemli pozitif korelasyon tespit edildi (pO.Ol). Çalışmada incelenen toplam 60 baş inekte kavile formasyonu gözlenen CL' ların görülme oranı % 25 olarak belirlendi. Kaviteli ve kavitesiz CL' a sahip inekler arasında plazma progesteron düzeyi. CL çapı ve CL ağırlığı arasında farklılık bulunamazken kaviteli CL' larda endokrin aktivite (plasma progesteron düzeyi (ng/ml) / CL ağırlığı (gr) düzeyi önemli oranda düşük bulundu (p0.05). Araştırmada incelenen 3 farklı CL tipi arasında endokrin aktivite yönünden farklılık bulunamadı.Sonuç olarak, CL' un kavite formasyonunun ultrasonografik olarak incelenmesinin yanısıra ağırlığı ve çapının da endokrin aktivitesi hakkında değerli bilgiler verebileceği kanısına varıldı.Öğe İneklerde Azaltılmış Dozda Cloprostenol Uygulamalarının Luteolitik Etkisinin Klinik Östrüs Belirtileri ve Plazma Progesteron Düzeyleri Yardımıyla İzlenmesi(Selçuk Üniversitesi, 1994) Tekeli, Tevfik; Aksoy, Melih; Çoyan, Kenan; Semacan, Ahmet; Özsar, Semin; Güven, Bülent; Alan, MuhammedIn this study, the effectiveness of a reduced dose of cloprostenol injected by intravulvosubmucosal route was eva/uated for esirus induction and luteolysis in cows. The study was carried out on 43 Brown Swiss cows, age ranging 3 to 5 years old. Firstly, all cows were injected 500 mcg of c/oprostenol by intramuscular route. On the 11 th. day, cows in the first group (n: 38). Were reinjected 125 mcg of cloprostenol by intravulvo submucosal route and the remaining cows in the second group (n:5) were administered an injection of 500 rncg cloprostenol intramusculary. Blood samples to determine the p/asma progesterone /eve/s were collected on the day of second injection and tour days thereafter from 9 and 5 cows in the first and second groups, respectively. According to the obtained results; esirus rates in first and second groups were 26.3 and 1 vO %,· respective/y. On the basis of the 7 cows inseminated in the first group. pregnancy rate was 57.1 %. Progesterone /eve/s of the cows in the first group decreased alter the second injection. Although a slight decrease in progesterone /eve/s was momtored in the cows with no /uteal regression, it was never below 1 nglml. The progesterone /eve/s of the cows in the first group with luteo/ysis showed a sharp decline compared to the control group. Asa result, application of 125 mcg of cloprostenol injection into vulvar submucosa induced estrus and luteolysis in cows. lt was concluded that the higher doses of cloprostenol administrations by intravulvo-submucosal injections may provide belter results in cows.Öğe Influence of Ejaculation Frequency on Sperm Characteristics, Ionic Composition and Enzymatic Activity of Seminal Plasma in Rams(Elsevier Science Bv, 2002) Kaya, Abdullah; Aksoy, Melih; Tekeli, TevfikInfluence of frequency of ejaculation on the ionic composition and enzymatic activity of the seminal plasma and the spermatozoa parameters was evaluated. Semen was collected from 8 rams for a period of 12 days in four successive phases with a gradually increasing semen collection intensity. Each phase continued for 3 days and rams were ejaculated 1, 3, 6 and 8 times per day during phase I, II, III and IV, respectively. Semen volume, sperm concentration and progressive motility rates decreased significantly with regards to elevated semen collection frequency. Though abnormal sperm morphology increased, no specific type of sperm abnormality or localization, i.e. acrosome, head, mid-piece or tail, was noted in response to increased ejaculation frequency. Dead sperm rate fluctuated between 3.4 and 6.2% during the study. The highest daily sperm output was reached by 36 times of collection per day. However, further increase of the ejaculation frequency to 8 times or decrease to once a day reduced daily sperm output (P < 0.05). Seminal plasma sodium and potassium concentration increased significantly with regard to elevated semen collection intensity. In contrast, calcium and magnesium levels reduced markedly (P < 0.01) from phase I to IV. Although aspartate amino transferase (AAT) and glutamic-pyruvic transaminase (GPT) activity increased (P < 0.01) in the seminal plasma the lactate dehydrogenase (LDH) level dropped (P < 0.01) in the later phases of higher intensity semen collection. Semen collection frequency may influence ionic composition and enzymatic activity in the seminal plasma in addition to the spermatozoa parameters and daily sperm output in the ram. (C) 2002 Elsevier Science B.V. All rights reserved.Öğe Influence of Sugar Supplementation of the Extender on Motility Viability and Acrosomal Integrity of Dog Spermatozoa During Freezing(Elsevier Science Inc, 2000) Yıldız, Cengiz; Kaya, Abdullah; Aksoy, Melih; Tekeli, TevfikInfluence of different sugars supplemented to the extender on the motility, viability and intact acrosome rates of dog spermatozoa during dilution, equilibration and freezing was studied. The ejaculate was divided into 10 aliquots, which were diluted 1:3 with TRIS-citric acid extender containing 240 mM TRIS, 63 mM citric acid, 8% (v/v) glycerol, 20% (v/v) egg yolk and 70 mM sugar, which was either fructose, galactose, glucose, xylose (monosaccharide), lactose, trehalose, maltose, sucrose (disaccharide) or raffinose (trisaccharide). No sugar was added to the extender in the control group. Extended semen samples were cooled to 5 degreesC over 45 min, packaged in 0.25-mL straws, equilibrated for 2 h at 5 degreesC and frozen in liquid nitrogen vapor. Samples were thawed by placing straws into 37 degreesC water for 30 sec. Motility, viable sperm and intact acrosome rates decreased gradually in all groups after equilibration and consecutively freezing (P<0.001). The type of sugar significantly effected motility, viability and acrosomal integrity during equilibration and freezing (P<0.05). Galactose, lactose, trehalose, maltose and sucrose reduced damaged acrosome percentages in equilibrated samples (P<0.05). Sugar supplementation did not enhance motility and viability during equilibration. The disaccharides, except lactose, reduced post-thaw dead sperm and/or damaged acrosome percentages without promoting post-thaw motility (P<0.01), whereas monosaccharides, especially fructose and xylose, improved motility (P<0.05) along with viability and intact acrosome rates (P<0.05). Trehalose, xylose and fructose significantly increased total active sperm rates (motility x live sperm rate x normal acrosome rate) compared to other sugars (P<0.01) and control (P<0.0001) in frozen thawed samples. Therefore, sugar supplementation of the extender influenced post-equilibration and post-thaw sperm quality, and the type or locality of protective impact of the sugar on dog spermatozoa vary according to type of the sugar.Öğe Konya Hayvancılık Merkez Araştırma Ensti?tüsü'ne Ai?t Çeşi?tli? Irklardan Koçların Spermatolojik Özelli?kleri? Üzeri?nde Araştırmalar(Selçuk Üniversitesi, 1994) Aksoy, Melih; Ataman, M. Bozkurt; Kaya, Abdullah; Tekeli, Tevfik; Karaca, FikretIn the present study dermatological features of Awassi, Akkaraman, Corriedale and Merino rams. a total of 20 animals. in a flock of Konya Central Animal Research Institute were determined. Semen samples of rarns were collected by artifical vagina and examined for semen volume, individual motility, sperm concentration, liveldead and abnormal spermatozoon rates. As a result, all features of the semen samples were in normal limits but there were some differences in characteristics between the breeds.Öğe Konya Merinosu koçlarında sperma kalitesi,testis ölçüleri ve kan testosteron düzeylerine ilişkin mevsimsel değişikliklerin araştırılması(1999) Kaya, Abdullah; Yıldız, Cengiz; Lehimcioğlu, Necdet Cankat; Ergin, Ali; Aksoy, MelihKonya Merinosu koçlarının sperma kalitesi, teslis ölçüleri ve plazma testosteron düzeyleri üzerine mevsimsel değişikliklerin etkileri belirlendi. Araştırma, Mart 1997 ile Şubat 1998 tarihleri arasında ilkbahar, yaz, sonbahar ve kış mevsimlerinde 3-4 yaşlı toplam 10 baş koç üzerinde gerçekleştirildi. Sperma örnekleri koçlardan suni vajen yardımıyla alındı ve her bir sperma örneğinde miktar, yoğunluk, motilite, ölü ve anormal spermatozoon oranları belirlendi. Koçların skrotal çevre uzunluğu (SC) ve teslis hacimleri (TV) ölçüldü ve her bir mevsimde plazma testosteron düzeylerinin belirlenmesi amacıyla kan örnekleri alındı. Koçların sperma kal'tesi sonbaharda arttı ve kış mevsiminde azaldı. SC değerleri mevsimlere göre değişti, en yüksek SC değeri sonbaharda ve en düşük kış mevsiminde şekillendi (p0.05). Yaz ve sonbahar mevsimlerinde elde edilen TV düzeyleri, ilkbahar ve kış ile karşılaştırıldığında önemli ölçüde yüksek bulundu (p0.05). Sonbaharda belirlenen plazma testosteron düzeyleri, yaz mevsiminde elde edilen değerden daha yüksekti (p0.05). Bununla birlikte en düşük testosteron düzeyi ilkbahar ve kış mevsimlerinde elde edildi. Sperma miktarı, yoğunluğu ve motililesinin, kendi aralarında pozitif korelasyon bulunmasına rağmen, ölü ve anormal spermatozoon oranları ile aralarında negatif korelasyon olduğu belirlendi (p0.05). SC ve TV düzeyleri ile sperma miktarı ve motilitesi arasında pozitif korelasyon tespit edildi, ancak, ölü ve anormal spermatozoon oranları arasında negatif korelasyon belirlendi (p0.05). Bu sonuçlar, Konya Merinosu koçlarında sperma kalitesi, teslis ölçüleri ve plazma testosteron düzeylerinin mevsimlere göre değiştiğini göstermektedir.
