Influence of Sugar Supplementation of the Extender on Motility Viability and Acrosomal Integrity of Dog Spermatozoa During Freezing
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Dosyalar
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Influence of different sugars supplemented to the extender on the motility, viability and intact acrosome rates of dog spermatozoa during dilution, equilibration and freezing was studied. The ejaculate was divided into 10 aliquots, which were diluted 1:3 with TRIS-citric acid extender containing 240 mM TRIS, 63 mM citric acid, 8% (v/v) glycerol, 20% (v/v) egg yolk and 70 mM sugar, which was either fructose, galactose, glucose, xylose (monosaccharide), lactose, trehalose, maltose, sucrose (disaccharide) or raffinose (trisaccharide). No sugar was added to the extender in the control group. Extended semen samples were cooled to 5 degreesC over 45 min, packaged in 0.25-mL straws, equilibrated for 2 h at 5 degreesC and frozen in liquid nitrogen vapor. Samples were thawed by placing straws into 37 degreesC water for 30 sec. Motility, viable sperm and intact acrosome rates decreased gradually in all groups after equilibration and consecutively freezing (P<0.001). The type of sugar significantly effected motility, viability and acrosomal integrity during equilibration and freezing (P<0.05). Galactose, lactose, trehalose, maltose and sucrose reduced damaged acrosome percentages in equilibrated samples (P<0.05). Sugar supplementation did not enhance motility and viability during equilibration. The disaccharides, except lactose, reduced post-thaw dead sperm and/or damaged acrosome percentages without promoting post-thaw motility (P<0.01), whereas monosaccharides, especially fructose and xylose, improved motility (P<0.05) along with viability and intact acrosome rates (P<0.05). Trehalose, xylose and fructose significantly increased total active sperm rates (motility x live sperm rate x normal acrosome rate) compared to other sugars (P<0.01) and control (P<0.0001) in frozen thawed samples. Therefore, sugar supplementation of the extender influenced post-equilibration and post-thaw sperm quality, and the type or locality of protective impact of the sugar on dog spermatozoa vary according to type of the sugar.
Açıklama
Anahtar Kelimeler
dog, semen, cryopreservation, sugar, extender
Kaynak
Theriogenology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
54
Sayı
4
Künye
Tekeli, T., Aksoy, M., Kaya, A., Yıldız, C., (2000). Influence of Sugar Supplementation of the Extender on Motility Viability and Acrosomal Integrity of Dog Spermatozoa During Freezing. Theriogenology, 54(4), 579-585. Doi: 10.1016/S0093-691X(00)00373-3
