Influence of Sugar Supplementation of the Extender on Motility Viability and Acrosomal Integrity of Dog Spermatozoa During Freezing

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dc.contributor.authorYıldız, Cengiz
dc.contributor.authorKaya, Abdullah
dc.contributor.authorAksoy, Melih
dc.contributor.authorTekeli, Tevfik
dc.date.accessioned2020-03-26T16:36:16Z
dc.date.available2020-03-26T16:36:16Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractInfluence of different sugars supplemented to the extender on the motility, viability and intact acrosome rates of dog spermatozoa during dilution, equilibration and freezing was studied. The ejaculate was divided into 10 aliquots, which were diluted 1:3 with TRIS-citric acid extender containing 240 mM TRIS, 63 mM citric acid, 8% (v/v) glycerol, 20% (v/v) egg yolk and 70 mM sugar, which was either fructose, galactose, glucose, xylose (monosaccharide), lactose, trehalose, maltose, sucrose (disaccharide) or raffinose (trisaccharide). No sugar was added to the extender in the control group. Extended semen samples were cooled to 5 degreesC over 45 min, packaged in 0.25-mL straws, equilibrated for 2 h at 5 degreesC and frozen in liquid nitrogen vapor. Samples were thawed by placing straws into 37 degreesC water for 30 sec. Motility, viable sperm and intact acrosome rates decreased gradually in all groups after equilibration and consecutively freezing (P<0.001). The type of sugar significantly effected motility, viability and acrosomal integrity during equilibration and freezing (P<0.05). Galactose, lactose, trehalose, maltose and sucrose reduced damaged acrosome percentages in equilibrated samples (P<0.05). Sugar supplementation did not enhance motility and viability during equilibration. The disaccharides, except lactose, reduced post-thaw dead sperm and/or damaged acrosome percentages without promoting post-thaw motility (P<0.01), whereas monosaccharides, especially fructose and xylose, improved motility (P<0.05) along with viability and intact acrosome rates (P<0.05). Trehalose, xylose and fructose significantly increased total active sperm rates (motility x live sperm rate x normal acrosome rate) compared to other sugars (P<0.01) and control (P<0.0001) in frozen thawed samples. Therefore, sugar supplementation of the extender influenced post-equilibration and post-thaw sperm quality, and the type or locality of protective impact of the sugar on dog spermatozoa vary according to type of the sugar.en_US
dc.identifier.citationTekeli, T., Aksoy, M., Kaya, A., Yıldız, C., (2000). Influence of Sugar Supplementation of the Extender on Motility Viability and Acrosomal Integrity of Dog Spermatozoa During Freezing. Theriogenology, 54(4), 579-585. Doi: 10.1016/S0093-691X(00)00373-3
dc.identifier.doi10.1016/S0093-691X(00)00373-3en_US
dc.identifier.endpage585en_US
dc.identifier.issn0093-691Xen_US
dc.identifier.issn1879-3231en_US
dc.identifier.issue4en_US
dc.identifier.pmid11071132en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage579en_US
dc.identifier.urihttps://dx.doi.org/10.1016/S0093-691X(00)00373-3
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17221
dc.identifier.volume54en_US
dc.identifier.wosWOS:000090120200008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYıldız, C.
dc.institutionauthorKaya, A.
dc.institutionauthorAksoy, M.
dc.institutionauthorTekeli, T.
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectdogen_US
dc.subjectsemenen_US
dc.subjectcryopreservationen_US
dc.subjectsugaren_US
dc.subjectextenderen_US
dc.titleInfluence of Sugar Supplementation of the Extender on Motility Viability and Acrosomal Integrity of Dog Spermatozoa During Freezingen_US
dc.typeArticleen_US

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