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Öğe The Comparison of Pregnancy Outcomes of Progesterone or Progesterone Plus Estradiol for Luteal Phase Support in Icsi-et Cycles(Elsevier Science Inc, 2003) Görkemli, Hüseyin; Aktan, Murat; Ak, Dilek; Duman, Selçuk; Açyürek, Cemalettin[Abstract not Available]Öğe Does fetal hepatocyte transplantation provide metabolic support immediately after surgically induced acute hepatic failure in rats?(1999) Dilsiz, Alaeddin; Köseoğlu, Burhan; Kaymakçı, Aytekin; Aktan, Murat; Duman, Selçuk; Erdoğan, EnderAkut karaciğer yetmezliği yüksek mortalite oranı ile alakalıdır. Akut karaciğer yetmezliği (AKY) olan hastalarda cerrahi yaklaşım ortotopik karaciğer transplantıdır. Ancak bu yaklaşım birçok risk ve zorluk içerir. Eğer yetmezliğin kritik döneminde uygun destek tedavisi verilirse karaciğer rejenere olma ve kendiliğinden iyileşme fırsatını bulabilir. Bu çalışmada %90 hepatektomi ile AKY yetmezliği oluşturulan Wistar Albino sıçanlarda intrasplenik fetal hepatosit transplantasyonunun metabolik destek sağlaması değerlendirildi. Sıçanlar aşağıdaki gibi dört gruba ayrıldı. Grup 1. AKY’den 4 ay önce fetal hepatositler (0.1 ml Fetal Hepatosit (FH) süspansiyonu, 2x106 hücre) dalak içine transplante edildi. Grup 2. Fetal hepatositler AKY ile aynı zamanda transplante edildi. Grup 3. Hank’in dengeli tampon solüsyonu dalak içine enjekte edildi ve 4 ay sonra AKY oluşturuldu. Grup 4. Hank’ın dengeli tampon solüsyonunun enjeksiyonu ile AKY oluşturulması aynı zamanda yapıldı. Mortalite oranları, kan glukoz seviyeleri ve dalağın histopatolojik görünümü değerlendirildi. Grup 2, 3 ve 4’deki hayvanların hepsi 5 gün içinde öldü, grup 1’de 2 hayvan öldü, beşi ise yaşadı. Grup 1’de kan glukoz seviyeleri diğerlerine göre anlamlı olarak yüksekti. Hepatositlerin makroskopik nodülleri ve dalak içindeki mikroskopik organize hepatositler sadece grup 1’de gözlendi. Grup 2, 3 ve 4 ile kıyaslanınca grup 1’deki anlamlı yaşamda kalma oranları fonksiyon gören hepatositlerin sağladığı desteğe bağlıdır. Sonuç olarak AKY’den önce yapılırsa fetal hepatosit transplantasyonu hayatta kalma oranını iyileştirmektedir.Öğe The Effects of Different Suture Techniques on Wound Healing in Abdomibnal Wall Closure(2001) Şahin, Mustafa; Karademir, Mehmet; Özer, Şükrü; Avşar, Fatih Mehmet; Çağlayan, Osman; Aksoy, Faruk; Aktan, MuratPurpose: The aim this study was to investigate the effects of different suture techniques on the healing of abdominal wall wound. Materials and Methods: 24 male Wistar albino rats were included in this study. The rats were divided into two groups. A 4 cm midline laparatomy was performed after ketamine HCl anesthesia. In Group I, the incision was sutured with 4/0 polypropylene interruptedly and in Group II, the wound was closed with 4/0 polypropylene continuously. The skin was sutured with an interrupted 4/0 silk suture. Then 2 cc of blood was withdrawn for biochemical and hematological tests by cardiac puncture. All the rats were killed on the 7th postoperative day. Tensile strength were measured and tissue samples were taken for hydroxyproline measurements and histopathological evaluation. Meanwhile intraabdominal adhesions were recorded. Results: Tensile strength was 751±31 g in Group I and 622±28 g in Group II. The difference was significant (p<0.05). Hydroxyproline levels were 3.13±0.15 µg/mg tissue in Group I and 2.81±0.15 µg/mg tissue in Group II. The difference was significant (p<0.05). Intraabdominal adhesions were found in 3 rats in Group I and in 6 rats in Group II. There was a significant difference between the two groups regarding tissue fibroblast numbers (p<0.05). There was no significant difference between serological and hematological tests in the two groups. Conclusion: Closing the abdominal wall wound with the interrupted suture technique gave better results than with the continuous suture technique.Öğe The Effects of Different Suture Techniques on Wound Healing in Abdominal Wall Closure(2001) Şahin, Mustafa; Karademir, Mehmet; Özer, Şükrü; Avşar, Fatih Mehmet; Çağlayan, Osman; Aksoy, Faruk; Aktan, MuratPurpose: The aim this study was to investigate the effects of different suture techniques on the healing of abdominal wall wound. Materials and Methods: 24 male Wistar albino rats were included in this study. The rats were divided into two groups. A 4 cm midline laparatomy was performed after ketamine HCl anesthesia. In Group I, the incision was sutured with 4/0 polypropylene interruptedly and in Group II, the wound was closed with 4/0 polypropylene continuously. The skin was sutured with an interrupted 4/0 silk suture. Then 2 cc of blood was withdrawn for biochemical and hematological tests by cardiac puncture. All the rats were killed on the 7th postoperative day. Tensile strength were measured and tissue samples were taken for hydroxyproline measurements and histopathological evaluation. Meanwhile intraabdominal adhesions were recorded. Results: Tensile strength was 751±31 g in Group I and 622±28 g in Group II. The difference was significant (p<0.05). Hydroxyproline levels were 3.13±0.15 ?g/mg tissue in Group I and 2.81±0.15 ?g/mg tissue in Group II. The difference was significant (p<0.05). Intraabdominal adhesions were found in 3 rats in Group I and in 6 rats in Group II. There was a significant difference between the two groups regarding tissue fibroblast numbers (p<O.05). There was no significant difference between serological and hematological tests in the two groups. Conclusion: Closing the abdominal wall wound with the interrupted suture technique gave better results than with the continuous suture technique.Öğe The impact of oral feeding on the severity of acute pancreatitis(CAHNERS PUBL CO, 1999) Şahin, Mustafa; Özer, Şükrü; Vatansev, Celalettin; Aköz, Mehmet; Vatansev, Hüsamettin; Aksoy, Faruk; Dilsiz, Alaattin; Yılmaz, Osman; Karademir, Mehmet; Aktan, MuratBACKGROUND: In the management of acute pancreatitis, oral feeding is prohibited and either enteral or parenteral feeding is commenced for the patients in an effort to not increase the secretion of the pancreatic enzymes. PURPOSE: This study was undertaken in an attempt to determine the impact of oral feeding on the severity of acute pancreatitis and to compare this impact with that of parenteral feeding. MATERIALS AND METHODS: Twenty-four female Sprague-Dawley rats were divided into two groups. In both groups, acute pancreatitis was induced by ligation of the main biliopancreatic duct. The rats in group I were fed orally and the rats in group II were fed parenterally, The rats were sacrificed at 48 hours, and blood samples were obtained from the heart upon exposure of the abdominal and thoracic cavities, The pancreas and the left lung were removed for histopathological examination, The levels of lactic dehydrogenase (LDH), serum glutamic oxaloacetic transaminase (SGOT), glucose, calcium and blood urea nitrogen, base deficit, partial oxygen pressure, leukocyte count, and hematocrit level among Ranson criteria and the level of amylase were measured, The pancreas and the lung were examined under a light microscope. RESULTS: The levels of LDH, SGOT, and calcium for the rats in group I were significantly higher when compared with the rats in group II (P <0.05), Similarly, the levels of amylase for the rats in group I were found to be higher when compared with the rats in group II, but the difference was not significant. Inflammatory changes observed in the pancreas were less severe whereas inflammatory changes observed in the lung were more severe for the rats in group I when compared with the rats in group II. CONCLUSIONS: The blood levels of the enzymes were adversely affected for the rats fed orally. In contrast, inflammatory changes observed in the pancreas were more severe for the rats fed parenterally, The study suggests that certain hormones released from the duodenum upon stimulation by oral nutrient intake lessens the severity of pancreatitis through protective effects on the pancreas, whereas the elevated levels of the enzymes cause endothelial damage resulting in destruction in distant organs such as the lung.Öğe Incidence of Chromosome 8, 10, X and Y Aneuploidies in Sperm Nucleus of Infertile Men Detected by FISH(Karger, 2000) Acar, Hasan; Kılınç, Mehmet; Çora, Tülin; Aktan, Murat; Taşkapu, HakanWe studied the frequency of aneuploidy in sperm nuclei of six infertile men with abnormal semen profile and normal karyotype, using fluorescence in situ hybridization (FISH) with DNA probes for chromosomes 8, 10, X and Y. The control group consisted of four healthy fertile men with normal karyotype and semen profiles. The purpose of this study was to determine whether there are differences between infertile male donors and control donors for: (1) the incidence of sex chromosome aneuploidy, and (2) the number of disomies for chromosomes 8, and 10 cosegregating with chromosomes X and Y. FISH analysis showed no significant differences of sex ratios of the sperm nuclei in and between infertile and control groups. The most significant abnormalities in the infertile group were clusters of sperm nuclei bearing XY and XYY. In addition, the incidence of disomic sperm nuclei for chromosomes 8 and 10 consegregating with sex chromosomes was not significantly different beween the patient and control groups, nor within them. However, the total frequency of aneuploid sperm nuclei was significantly different beween the infertile group and the control group. We observed a significant excess of sperm nuclei bearing chromosome 10 along with disomy for chromosome Y (10YY). In conclusion, our results from FISH analysis demonstrate a significantly increased frequency of aneuploidy for the sex chromosomes in sperm nuclei from infertile men. Therefore it may be concluded that infertility is a risk factor for sex chromosome aneuploidy in sperm nuclei.Öğe Primer Infertil Çiftlerde Uygulanan Intrauterin Inseminasyonda Rekombinant FSH İle Klomifen Sitrat Sonuçlarının Karşılaştırılması(2002) Görkemli, Hüseyin; Aktan, Murat; Ak, Dilek; Duman, Selçuk; Akyürek, CemalettinAmaç: İntrauterin inseminasyon slkluslarında Klomifen sürat (KS) ve düşük doz rekombinant FSH (rFSH)'in gebelik başarısinı karşılaştırmak. Yer: Selçuk Üniversitesi Tıp Fakültesi Yardımcı Üreme Teknikleri Ünitesi Materyal ve Metod: Toplam 55 kadına en fazla 2 siklus olmak üzere ya KS (31 siklus) ya da r FSH (51 siklus) ile ovulasyon indüksiyonu yapıldı. KS siklusun 5. günü başlandı ve 100 mg 5 gün kullanıldı, r FSH 501 Ü/gün dozunda siklusun 2. veya 3. günü başlandı ve en az 18 mın'lik öncü birfollikül elde edilene kadar devam edildi. Bulgular: Hasta başına düşen gebelik oranı KS-IUI sikluslarinda %57.l (12/21), rFSH-IUI sikluslarinda %52.9 (18/34) olarak bulundu. Bu istatistiksel olarak anlamlı degildi.(p0.05) Siklus başına düşen gebelik oranları KS-IUI sikluslarinda %38.7 (12/31) ve rFSH-IUI sikluslarinda %35.2 (18/51) olarak bulundu. Bu sonuçlar istatistiki olarak anlamlı değildi.(p0.05) Sonuçlar: Uygun hasta seçimi yapıldığı zaman KS-IUf uygulamasının infertilite tedavisinde en az rFSH-IUI uygulaması kadar başarılıdır. Tedavi maliyetleri gözönüne alındığında, daha önce klomifen sitrat tedavisi uygulanmamış olan hastalarda gonadotropinlere başlamadan önce birkaç siklus KS denenmesinin uygun olacağını düşünmekteyiz.Öğe Sıçan Defonksiyone İnce Barsağı Üzerinde Octreotid'in Trofik Etkisi(1998) Dilsiz, Alaeddin; Kaymakçı, Aytekin; Aktan, Murat; Karabacak, H. İbrahim; Kartal, AdilÇalışmamızda sıçanların defonksiyone ince hamağı, üzerinde octreotid'in (OCT, uzun etkili somatostalin analoğıı) etkisini ortaya koymak amaçlanmıştır: Çalışma 30 sıçanın defonksiyone ince karsağı üzerinde yapıldı. 15 cm uzunluğunda damar yapısı korunmuş jejunum ansı hamaktan izole edilerek ınııkus fıstülü şeklinde iki ucu karna ağızlaştırıldı. Barsak devamlılığı jejuııojejunostomi ile sağlandı. Sıçanlar her biri 15'er denekten oluşan iki gruba ayrıldı. Tüm hayvanlar oral yoldan izokalorik ve izonitmjenik olarak parenteral beslenme solüsyonu ile beslendi. Buna ek olarak ikinci gruba 5 (.ig/kg/gün dozda octreotid asetat verildi. Birinci grup kontrol grubunu oluşturdu. Üçüncü haftanın sonunda tüm sıçanlara laparolomi yapılarak defonksiyone barsak segmentleri lıislopatolojik inceleme için rezeke edildi. Bu segmentlerde villııs boyları ölçülde ve goblet hücreleri sayıldı. İkinci grupla, defonksiyone segmentin villııs boylan kontrol grubuna göre anlamlı olarak artma saptandı (p0.001), goblet hücre sayısı ise kontrol grubuna göre anlamlı olarak azalmıştı (]().001). Sonuç olarak OCT izole barsak segment! villıısları üzerinde pozitif trafik etkiye sahipken goblet hücrelerinde bu etkiyi göstermemektedir.Öğe Tavşan Kornea Alkali Yanıok Modelinde Pentoksifilinin Yara İyileşmesine Etkisi(2002) Özkağnıcı, Ahmet; Zengin, Nazmi; Aktan, MuratAMAÇ: Hücre ve dokuda cAMP seviyesini yükselten bir ilaç olan pentoksifilinin kornea alkali yanıklarında yara iyileşmesi üzerine etkisinin araştırılması. GEREÇ VE YÖNTEM: Çalışmada ortalama 3000 gram ağırlığında her iki cinsten 10 tavşanın 20 gözü kullanıldı. 1 N NaOH emdirilmiş filtre kağıdı kornea üzerine bırakılarak alkali yanık oluşturuldu. Gözlerin hepsine deksametazon ve tobramisin sülfat damla başlandı. Ek olarak 1 ml (20 mg) pentoksifilin sağ gözlere subkonjonktival olarak enjekte edildi, sol gözler kontrol olarak kullanıldı. Çalışmanın 10. gününde tavşanlar dekapite edildi. Kornealar tümüyle eksize edilerek hematoksilen eosin ile boyandı. Işık mikroskobuyla epitel rejenerasyonu, fibroblast proliferasyonu ve inflamatuar hücre invazyonu yönünden incelendi. BULGULAR: Çalışma ve kontrol gözleri arasında epitel rejenerasyonu, fibroblast proliferasyonu ve inflamatuar hücre invazyonu açısından anlamlı fark bulunmadı. SONUÇ: Çalışmamızın sonuçları pentoksifilinin kornea alkali yanık modelinde yara iyileşmesi üzerine etkinliğinin olmadığını düşündürmektedir. Ancak optimal dozun ve uygulama yolunun belirlenmesi amacıyla daha ayrıntılı çalışmalar yapılması gerekmektedir.Öğe Treatment of Intestinal Reperfusion Injury Using Antioxidative Agents(W B SAUNDERS CO-ELSEVIER INC, 1998) Günel, Engin; Çağlayan, Fatma; Çağlayan, Osman; Dilsiz, Alaeddin; Duman, Selçuk; Aktan, MuratBackground/Purpose: A number of antioxidants are present in human tissues, which comprise the secondary defence system against the oxygen-free radicals and oxidative stress. Therefore, this study was undertaken in an animal model to investigate the effects of antioxidants such as vitamin E, vitamin C, methyl prednisolone, and mannitol, on the treatment of intestinal reperfusion injury when given during ischemia. Methods: The study was performed on six groups of animals, each composed of six rabbits: Group I, mesenteric ischemia group; group R, ischemia-reperfusion group; group E, ischemia-reperfusion plus Vitamin E treatment; group C, ischemia-reperfusion plus vitamin C treatment; group CS, ischemia-reperfusion plus corticosteroid treatment; and Group M, ischemia-reperfusion plus mannitol treatment. Malondialdehyde (MDA) and glutathione (GSH) levels of the intestinal mucosa were assayed to reflect the free radical formation. Results: Mucosal injury scores in the M and C groups were significantly lower than the R group (P < .01 and P < .01, respectively), and in the E and CS groups, mucosal injury scores were not significantly different from R group. MDA levels in the M and C groups were significantly lower than the R group (P < .01 and P < .01, respectively). GSH levels in the E, C, and M groups, were significantly higher than R group (P < .01, P < .05, and P < .01, respectively). Conclusion: The results of this study showed that antioxidant drugs, such as vitamin C and mannitol, may play a role in attenuating reperfusion injury of the gut demonstrated by depression of tissue MDA levels and by elevation of tissue GSH levels.
