Sıçan Defonksiyone İnce Barsağı Üzerinde Octreotid'in Trofik Etkisi
Küçük Resim Yok
Tarih
1998
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Çalışmamızda sıçanların defonksiyone ince hamağı, üzerinde octreotid'in (OCT, uzun etkili somatostalin analoğıı) etkisini ortaya koymak amaçlanmıştır: Çalışma 30 sıçanın defonksiyone ince karsağı üzerinde yapıldı. 15 cm uzunluğunda damar yapısı korunmuş jejunum ansı hamaktan izole edilerek ınııkus fıstülü şeklinde iki ucu karna ağızlaştırıldı. Barsak devamlılığı jejuııojejunostomi ile sağlandı. Sıçanlar her biri 15'er denekten oluşan iki gruba ayrıldı. Tüm hayvanlar oral yoldan izokalorik ve izonitmjenik olarak parenteral beslenme solüsyonu ile beslendi. Buna ek olarak ikinci gruba 5 (.ig/kg/gün dozda octreotid asetat verildi. Birinci grup kontrol grubunu oluşturdu. Üçüncü haftanın sonunda tüm sıçanlara laparolomi yapılarak defonksiyone barsak segmentleri lıislopatolojik inceleme için rezeke edildi. Bu segmentlerde villııs boyları ölçülde ve goblet hücreleri sayıldı. İkinci grupla, defonksiyone segmentin villııs boylan kontrol grubuna göre anlamlı olarak artma saptandı (p0.001), goblet hücre sayısı ise kontrol grubuna göre anlamlı olarak azalmıştı (]().001). Sonuç olarak OCT izole barsak segment! villıısları üzerinde pozitif trafik etkiye sahipken goblet hücrelerinde bu etkiyi göstermemektedir.
In this study, our aim was to investigate the effect of'octreotide (OCT, a longacting somatostatin analogue) in the defunctioned small bowel of rat.The study was performed on isolated loop of small bowel in 30 rats. A vascularizedjejunal loop, 15 cm in length, was prepared and separated from the intestine and its two ends brought out through the abdominal wall just as mucous fistula. Intestinal continuity was restored by jejunojejimostmy. The rats were divided into two groups, each of which had 15 animals. All groups had parenteral nutrition solution isocalorically and isonilrogenically by oral route. In addition to this, the second group received octreolide acetate (5 /.tg/kg/day). The first group acted as the control. At the end of the third week, all rats were under laparotomy. The isolated loop was resected for histological examination for length of villi and mucin (the number of goblet cells) were measured. In the second group, the length of villi increased significantly (p<0.001) in isolated loops compared with controls, but the number of goblet cells were decreased significantly (p<0.001). In conclusion, OCT has positive trophic effect on isolated bowel villi but no effect on goblet cells.
In this study, our aim was to investigate the effect of'octreotide (OCT, a longacting somatostatin analogue) in the defunctioned small bowel of rat.The study was performed on isolated loop of small bowel in 30 rats. A vascularizedjejunal loop, 15 cm in length, was prepared and separated from the intestine and its two ends brought out through the abdominal wall just as mucous fistula. Intestinal continuity was restored by jejunojejimostmy. The rats were divided into two groups, each of which had 15 animals. All groups had parenteral nutrition solution isocalorically and isonilrogenically by oral route. In addition to this, the second group received octreolide acetate (5 /.tg/kg/day). The first group acted as the control. At the end of the third week, all rats were under laparotomy. The isolated loop was resected for histological examination for length of villi and mucin (the number of goblet cells) were measured. In the second group, the length of villi increased significantly (p<0.001) in isolated loops compared with controls, but the number of goblet cells were decreased significantly (p<0.001). In conclusion, OCT has positive trophic effect on isolated bowel villi but no effect on goblet cells.
Açıklama
Anahtar Kelimeler
Genel ve Dahili Tıp
Kaynak
Türkiye Klinikleri Tıp Bilimleri Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
6
Künye
Dilsiz, A., Kaymakçı, A., Aktan, M., Karabacak, H. İ., Kartal, A., (1998). Sıçan Defonksiyone İnce Barsağı Üzerinde Octreotid'in Trofik Etkisi. Türkiye Klinikleri Tıp Bilimleri Dergisi, 18(6), 398-401.
