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Öğe Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements(SOC CHILENA ANATOMIA, 2012) Aksoy, Emine; Aktan, Tahsin Murad; Duman, Selcuk; Cuce, GokhanThe aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of the study, smears were stained with Hematoxylin Eosin (HE), Toluidin Blue (TB), Giemsa, Wright, ferrous Weigert haematoxylin stain, Orange G, eosin-aniline blue dye, Shorr Method, Papanicolau, Berg Method, Light Green stain, Acridine Orange (AO) and Janus Green dyes. In the second part of the study, smear preparations of 10 patients with normozoospermic were stained with HE, Toluidin Blue (TB), Shorr Method and Papanicolau. Four measurements were made including the middle piece, head length- head width and tail length for 200 spermatozoa with normal morphology. Comparisons were made between the stains that which showed a better morphology. Condensation assessment was not possible in smears stained with Shorr, Berg, Method, AO. Better assessment of condensation could be made in other stains. In the second part the smallest values belonged to of TB stain according to measurements of head of the spermatozoa. There was a significant difference at the head length with TB stain. Although measurements of Shorr and Papanicolau are close to each other and the largest values belonged to Papanicolau dye. It was concluded that measurement values in human sperm morphology could alter with the used staining method.Öğe Bacterial translocation and intestinal injury in experimental necrotizing enterocolitis model(COMENIUS UNIV, 2012) Çiftçi, İlhan; Özdemir, Mehmet; Aktan, Tahsin Murad; Asian, K.Objective: To study the occurrence of bacterial translocation and to assess the impact of breasffeeding on bacterial translocation in the animal model of necrotizing enterocolitis. Methods: A total of 20 neonate Sprague-Dawley rats were enrolled in the study. Rats were randomly allocated into either control or study group just after birth. Ten newborn rats in the control group were left with their mother to be breast-fed. In contrary, necrotizing enterocolitis group consisted of neonates that were separated from their mothers, housed in an incubator and were gavaged with a special rodent formula three times daily. Survival rates, weight changes, and morphologic scoring obtained after microscopic evaluation were determined as microbiologic evaluation criteria. Results: All the rats in the control group survived, while 1 (10 %) rat died in the necrotizing enterocolitis group. Mortality rates of the two groups were similar. All the formula-fed animals in the necrotizing enterocolitis group had significant weight loss compared to the breast milk-fed rats in the control group (p<0.05). A total of 7 (70 %) and 2 (20 %) E. coli growths were identified in the bowel lumen, liver, and spleen of necrotizing enterocolitis and control groups, respectively. This difference was statistically significant. In peritoneal smear cultures, a total of 3 (30 %) growths were detected in the necrotizing enterocolitis group and 1 (10 %) growth in the control group. Conclusion: As the result of a disturbance in the intestinal flora and impairment of the intestinal barrier in necrotizing enterocolitis, microrganisms in the bowel pass through the intestinal barrier and reach the liver and the spleen via the hematogenous route. This condition is closely related to the impairment of physiological and functional features of the intestinal barrier and is independent from the degree of intestinal injury. Bacterial translocation should be remembered in cases suspected of necrotizing enterocolitis, and a rapid and effective treatment algorithm should be applied in such circumstances (Tab. 3, Fig. 3, Ref. 21). Full Text in PDF www.elis.sk.Öğe Cellular Cardiomyoplasty: Arterial Cells-Stem Cells Transplantation(Springer Netherlands, 2012) Tokaç, Mehmet; Aktan, Tahsin MuradCardiovascular disease is the leading cause of mortality and morbidity all over the world. Current treatments in cardiovascular disease do not prevent the cell loss and have no contribution to regeneration, although these therapies provided an improvement in the survival. Cardiac repair by stem cells promises regenerating damaged myocardium by reconstructing the injured heart from its component parts. Up to date, variety of stem cells including BM mononuclear, adipocyte progenitor cells, fibroblasts, smooth and strait muscle cells, circulating progenitor cells, embryonic stem cells, cord blood cells etc., have been used for cardiac repair experimentally and some at clinically. There is no consensus which cell should be use for cardiac repair. We strongly suggest that vascular wall cells seem to be a good candidate for cardiomyoplasty. © Springer Science+Business Media B.V. 2012.Öğe Comparison of multiple dose GnRH antagonist and minidose long agonist protocols in poor responders undergoing in vitro fertilization: a randomized controlled trial(SPRINGER HEIDELBERG, 2008) Tazegül, Aybike; Görkemli, Hüseyin; Özdemir, Suna; Aktan, Tahsin MuradObjective To investigate the efficacy of gonadotropin releasing hormone antagonist (GnRH) in poor responders undergoing in vitro fertilization. Study design Ninety-six patients with poor ovarian response in previous treatment cycles were prospectively randomized into two groups. Forty-four patients were stimulated with GnRH antagonist multidose protocol and 45 patients received a standard long agonist protocol. Ovarian response was evaluated by transvaginal ultrasound and hormonal parameters. Cycle characteristics and treatment outcomes were statistically compared between groups. Results There was significantly reduced duration of stimulation and consumption of gonadotrophins in the antagonist group when compared to the agonist group. The estradiol concentrations on the day of human chorionic gonadotropin (hCG) injection, the number of oocytes retrieved, and the number of embryos transferred were similar for both groups. In the antagonist group, eight (18.1%) ongoing pregnancies were achieved and in the agonist group, ten (22.2%) clinical pregnancies were achieved but the difference was not statistically significant. Conclusions The present study was not powered to detect clinically relevant differences between two protocols in outcomes such as pregnancy rate, with confidence.Öğe Cryobiology [Kriobiyoloji](2000) Aktan, Tahsin Murad; Çıray, N.; Tosun, M.; Duman, S.; Erdoğan, E.By the advances and understandings in physics and mammalian cell biology, cryobiology makes us able to store our valuable biological materials and use them when wanted. The spectrum of materials is wide-spread from haploid gamete cells to cells processed via genetic engineering. The main tissues properly cryopreserved are skin grafts, cornea, tendon, bone, heart valve and bone marrow. It is important to know the basic mechanisms of freezing a biological specimen. The effects of mechanical damage caused by ice crystallisation and deleterious hyperosmotic medium must all be considered. To support cells during freezing temperatures, cryoprotectants have been searched. A proper freezing protocol needs effective seeding for preventing ice damage. The best indicator for a proper freezing protocol is a high post thaw viability rate. Also it is important to be careful while handling liquid nitrogen. Cryobiology can help diverse medical applications for example by the help of magnetic resonance imaging cancerous tissues can be treated. There are also some alternatives for cryobiology as anhydrobiology and vitrification. In this article the basic aspects of cryobiology are mentioned.Öğe Effects of balneotherapy on the number of blood leukocytes(2011) Cüce, Gökhan; Duman, Selçuk; Aktan, Tahsin Murad; İnce, BetülObjective: In recent years the use of mineral waters has become widespread throughout the world increasingly. There are studies indicating that the water temperature and the number of balneotherapy sessions are important. We aimed to investigate the effect of hyperthermia as a result of the balneotherapy sessions on the number of blood leukocytes in blood smears of humans. Material and Method: Balneotherapy sessions had applied in spa of Governor Saim Cotur (in Central Ilica town of Kahramanmaras). Patients entered the pool and stayed one hour a day. They waited for 1 hour in thermal water to neck level. Their ages were between 21-65 years and they did not have an infectious disease primarily. Blood samples were collected from 55 persons without distinction of sex. Groups were created according to the number balneotherapy sessions (1 hour, 3 hour, 5 hour, 10 hour). Blood smears were stained with May Grunwald Giemsa stain and 100 cells were counted at random areas. Monocyte, lymphocyte, eosinophil and neutrophil counts were determined separately. Group numbers were evaluated statistically and blood cells were evaluated morphologically. Results: There were significant differences between the groups in terms of the number of monocytes (p=0.001) and eosinophils (p=0.004). These significant differences were seen only the group which balneotherapy session applied 1 hour. There was no change in morphology of erythrocytes and leukocytes. Conclusion: One hour hyperthermal stress increases the number of monocytes and eosinophils in humans. Copyright © 2011 by Türkiye Klinikleri.Öğe Effects of Lipokit (R) Centrifugation on Morphology and Resident Cells of Adipose Tissue(SOC CHILENA ANATOMIA, 2013) Duman, Selcuk; Aktan, Tahsin Murad; Cuce, Gokhan; Cihantimur, Bulent; Tokac, Mehmet; Akbulut, HabipThe aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental step in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C-showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit (R) machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.Öğe Effects of nitric oxide synthase inhibition on intestinal damage in rats with experimental necrotizing enterocolitis(GEORG THIEME VERLAG KG, 2004) Çiftçi, İlhan; Dilsiz, A; Aktan, Tahsin Murad; Gürbilek, M; Duman, SelçukIn the inflamed intestinal mucosa of necrotizing enterocolitis (NEC), nitric oxide (NO) generated by inducible nitric oxide synthase (iNOS) may contribute to the pathogenesis of local intestinal damage. To study the importance of iNOS for the pathogenesis of NEC, the effects of selective (aminoguanidine, AG) and nonselective (1-nitroarginine methyl ester, L-NAME) iNOS inhibitors on intestinal morphologic changes were assessed in neonatal rats with experimental NEC. The neonatal rats were randomized into one of the five treatment groups. The control group consisted of rats that were breast-fed. The NEC group, consisting of neonates separated from their mothers, were gavaged with a special rodent formula to produce NEC. Rats in the sham, the AG, and the L-NAME groups were gavaged in a similar fashion to those in the NEC group; in addition, they were treated with 0.9% saline, 10 mg/kg/day AG, and 10 mg/ kg/day L-NAME, respectively. The rats were sacrificed on day 4, and the last 4 cm of terminal ileum was harvested for morphological studies and detection of nitrite and nitrate levels in tissue. The animals in the NEC and sham groups showed various degrees of intestinal inflammatory changes and increased tissue levels of nitrite and nitrate compared to those in the control group. Both AG and L-NAME treatment decreased the tissue levels of these nitrogen oxides, but the inflammatory changes of the intestine appeared to be attenuated only in the AG treated animals. L-NAME treatment did not improve the intestinal damage and increased mortality. These results may indicate that NO synthesized by iNOS plays a pathogenic role in formula-fed induced NEC and that inhibition of iNOS improves intestinal inflammatory damage.Öğe Enteral Glutamine Supplementation and Dexamethasone Attenuate the Local Intestinal Damage in Rats With Experimental Necrotizing Enterocolitis(Springer-Verlag, 2003) Dilsiz, Alaeddin; Çiftçi, İlhan; Aktan, Tahsin Murad; Gürbilek, Mehmet; Karagözoğlu, EmrahThe pathogenesis of necrotizing enterocolitis (NEC) presumptively is due to an inappropriate intestinal epithelial immunologic response of immature gut to luminal stimuli. Glutamine is essential for intestinal crypt cell proliferation and enhances the cellular response to growth factors. We aimed to test the hypothesis that the supplementation of enteral feedings with glutamine may stimulate an immature intestine and decrease the intestinal inflammatory change in NEC. Immediately after birth, the neonatal rats were weighed and randomized into one of four treatment groups. Group 1 consisted of rats whom were breast-fed. Group 2 (NEC group) consisted of neonates whom were fed with a special rodent formula. Rats in groups 3 and 4 were fed in a similar fashion to those in group 2, and glutamine 0.3 mg/kg per day and dexamethasone 0.5 mg/kg per day were added to their formula, respectively. The neonatal rats were weighed and killed on day 4: the last 4 cm of terminal ileum was harvested for morphological studies and detection of nitrite and nitrate levels in tissue. The animals in the NEC group showed various degrees of inflammatory changes similar to clinical NEC. The inflammatory changes of the intestine appeared to be attenuated in both glutamine- and steroid-treated animals compared to those in the NEC group. Only steroid treatment decreased the tissue levels of these nitrogen oxides that were increased in rats in the NEC group. We herein provide evidence that maturational agents such as glutamine and dexametasone can attenuate the local intestinal inflammatory damage in experimental NEC. These findings support the hypothesis that the gut immaturity in premature infants represents a risk factor for NEC.Öğe Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi(2009) Aksoy, Emine; Aktan, Tahsin Murad; Duman, Selçuk; Dursunoğlu, Duygu; Cüce, GökhanAmaç: Oligozoospermili ve teratozoospermili hastalarda Işık Mikroskobu düzeyinde sperm morfolojisi ve sperm DNA kondansasyonunun değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: oligozoospermili (n: 20) ve teratozoospermili (n: 25) hastalardan alınan seminal plazma örnekleri PBS ile 2 kez yıkandı ve smear hazırlandı, metil alkol ile fikzasyonu hazırlandı. Hematoksilen-Eosin, Toluidin-Blue, Giemsa ve Wright özel histolojik boyaları ile boyandı. Işık mikroskobunda 200 sperm hücresi sayılıp kondanse ve dekondanse başa sahip olan spermatozoalar belirlendi. Bulgular: Bu dört boya ile yapılan yaymalarda kondanse spermler, Hematoksüen-Eozin ve Toluidin Blue boyası ile boyanmış preperatlarda daha kolay ve net görüldü. İstatistiksel olarak dört boya ile kondansasyon değerlendirmesinde hasta grupları arasında fark olmadığı görüldü. Ayrıca oligozoospermili ve teratozoospermili hasta grupları için boyalar kendi içinde karşılaştırıldığında fark olmadığı görüldü. Ancak güven aralığı grafiğine bakıldığında; teratozoospermili hastaların oligozoospermili hastalara göre özellikle de Toluidin blue ile boyanmış grubun belirgin ölçüde homojen dağılım göstermesi; teratozoospermili hasta grubunun daha güvenilir kondanse sperm sağladığını gösterdi Sonuç: Sperm kondansasyon değerlendirmesi için toluidin blue boyası en güvenilir boyalardan bindir. Oligozoospermili ve teratozoospermili hastalar arasında ve bu dört boya arasında sperm nükleer kondansasyonunda fark yoktur. Ancak intrastoplazmik sperm injeksiyonu için kondanse sperm seçimi yapılırken oligozoospermili hastalara göre teratozoospermili hastalar daha güvenilir sonuç verebilir.Öğe Fötal sıçan hepatosit süspansiyonunun allograftik erişkin dalak paranşimine transplantasyonu(Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü, 1996) Aktan, Tahsin Murad; Cüce, HasanOldukça yüksek bir raorbidite ve mortaliteye sahip olan akut karaciğer yetmezliğinde akut kritik dönem geçici olarak bile olsa sağlanacak hepatik desteğin çok önemli olduğu bilinmektedir.Akut karaciğer yetmezliğinde taze olarak hazırlanan hepatosit süspansiyonunun en azından geçici bir destek olduğu tesbid edilirken son dönem karaciğer hastalığı ve yenidoğanların ilgili genetik hastalıklarında yegane seçenek olarak gözlenen orthotropik karaciğer nakline alternatif olarak hepatoselüler transplantasyon gündeme gelmiştir.Bu transplantasyonda fötal dönem eğer uygun şekilde değerlendirilirse zayıf antijenik özelliklere sahip olan hücrelerin nakli doku reddi ve alıcıda immunsüpresyon uygulamasını ortadan kaldırmaktadır.Çalışmada hücrelerin daha iyi tutunma şartları imkanını araştırmak için oluşturulan dalak damarlarının bağlanması ve hepatokteminin sağlıyacağı hepatotrofik faktör salgılanmasının olumlu etkileri, deney guruplarında haftalara bağlı kalmaksızın canlı fötal hepatositlerin kırmızı pulpada dağılmış olması bulgusunun ortak olması nedeniyle gruplar arası farklılık olmadığı sonucuna götürmüştür.Uygulamada allograftik fötal hücreler immunsüpresyon yapılmadan enjekte edildi ve alıcı organ olan dalakta doku reddi gözlenmedi. Ekimi yapılan fötal hücreler için dalağın uygun bir yatak olduğu anlaşılmıştır. Yapılan işlemler bir- ekip çalışmasını gerektirmekte olup uzun bir deneyim sahfasından sonra amaçlanan metod gerçekleşmiştir.Öğe A healthy baby from a cooled incubator(2004) Aktan, Tahsin Murad; Görkemli, Hüseyin; Duman, Selçuk[Abstract not Available]Öğe Improvement in embryo quality and pregnancy rates by using autologous cumulus body during icsi cycles(2011) Aktan, Tahsin Murad; Görkemli, Hüseyin; Gezginç, Kazım; Saylan, Aslı; Duman, Selçuk; Yılmaz, Fatma YazıcıAmaç: Bütünlüğü bozulmamış kumulus hücre kitlesi (BBKHK) hem embriyo kültürüne hem de embriyo transfer aşamalarında kullanımının gelişen embriyonun kalitesine ve gebelik oranlarına bir katkı sağlayıp sağlamadığını araştırmak. Gereç ve Yöntemler: Çalışmaya kısırlık sorunu ile başvuran ve ICSI işlemine alınan toplam 133 çift dahil edildi, 67 tanesine BBKHK ilavesi yapıldı (çalışma grubu) ve 66 tanesine standart tedavi yapıldı (kontrol grubu). İşlemde kullanılan BBKHK’nın elde edilmesi için sadece kumulus korona oosit kompleksinden basit bir kesme işlemi yapıldı. Çalışma kontrollü olgu şeklinde dizayn edildi. Bulgular: Çalışmaya dahil edilen her iki grubun yaş, östrodiol seviyeleri ve transfer günü endometrium kalınlığı özellikleri aynı idi (p0.05). Çalışma grubu kontrol grubu ile karşılaştırıldığında embriyo kalitesi (3.11.4 vs 2.41.1, p0.03), implantasyon oranları (53.7% vs 34.8%, p0.02) ve kalp atımı ultrasound teyidi ile devam eden gebelik oranlarında (44.7% vs 27.2%, p0.04) anlamlı farklılıklar ortaya çıktı. Sonuçlar: Embriyo kalitesi ve gebelik oranları BBKHK ilavesi ile arttırılabilir. Bu işlem zaman ve maddi maliyet artışı getirmeden sadece basit bir girişim ile kısırlık tedavisine önemli katkıda bulunmaktadır. (J Turkish-German Gynecol Assoc 2011; 12: 162-7)Öğe Improvement in embryo quality and pregnancy rates by using autologous cumulus body during icsi cycles(AVES, 2011) Aktan, Tahsin Murad; Görkemli, Hüseyin; Gezginç, Kazım; Saylan, Aslı; Duman, Selçuk; Yılmaz, Fatma YazıcıObjective: To determine whether the addition of intact cumulus cell mass (ICM) to both embryo culture (EC) and embryo transfer (ET) improves embryo quality and pregnancy rates. Material and Methods: A total of 133 infertile couples were included, of which 67 received ICM (study group) and 66 did not (control group). The ICM was obtained from a simple cutting of the cumulus corona oocyte complex (CCOC). A case control study design was used. Results: The clinical characteristics of the two groups before the embryo culturing step were similar with respect to age, estradiol level on the day of hCG and endometrial thickness on the day of embryo transfer (p>0.05). On the other hand study group with ICM had higher number of high quality embryos (3.1±1.4 vs 2.4±1.1, p=0.03), higher implantation rate (53.7% vs 34.8%, p=0.02) and higher ultrasound confirmation of gestational sac and fetal heart beat as ongoing pregnancy rates (44.7% vs 27.2%, p=0.04) compared to the control group without ICM. Conclusion: Addition of ICM improves embryo quality and pregnancy rates. This is a cost-and time-effective simple procedure that shows great promise for the improvement of infertility treatment. © 2011 by the Turkish-German Gynecological Education and Research Foundation.Öğe Increased Fat Graft Survival with Mesenchymal Stem Cell Recruiting Effect of PRP: In Vitro and In Vivo Study of Application Techniques(Selçuk Üniversitesi, 2022) Yıldıran, Gökçe; Çiçek, Gülsemin; Artaç, Hasibe; Uçaryılmaz, Hülya; Duman, Selcuk; Sütçü, Mustafa; Aktan, Tahsin Murad; Tosun, Zekeriya; Akdağ, Osman; Çelik, Z. EsinAim: Adding platelet-rich plasm (PRP) or mesenchymal stem cells are the most accepted methods to increase fat graft’s permanence. However, there is no consensus on timing and whether the effect of stem cells or PRP is observed more in the recipient area or in the donor area. It is aimed to present the application method and localization of PRP to be combined with fat graft to increase the survival. Material and Methods: in vitro part: Fat grafts were kept in a medium containing PRP. Cell output from the fat graft to the Petri dishes was examined every day. The time to recruit the maximum number of stem cells to the medium was determined (day 10). in vivo part: Eight group of rats were received PRP in different concentrations either to recipient or donor areas of fat grafts. After ten days, fat grafts were transferred to the recipient area. Ninety days after the transfer, histopathological examination and immunohistochemical staining were performed. Results: The group which had received the full-dose PRP to the recipient area showed the highest cellular integrity and vascularity (p< 0.05). Vascularity was superior in the half-dose PRP group compared to the control group (p< 0.05); cellular integrity did not increase. Donor site groups did not show increase in cellular integrity. Conclusion: Although PRP starts to increase fat graft permanence by increasing vascularity, it acts by preserving the cellular integrity of the fat cells as the concentrations increase. The PRP injection to the recipient area 10 days before fat grafting may provide higher survival rates.Öğe Intra-articular platelet-rich plasma injection for the treatment of temporomandibular disorders and a comparison with arthrocentesis(CHURCHILL LIVINGSTONE, 2015) Hanci, Mustafa; Karamese, Mehtap; Tosun, Zekeriya; Aktan, Tahsin Murad; Duman, Selcuk; Savaci, NedimBackground: Temporomandibular joint (TMJ) internal derangements are progressive painful conditions and cause joint dysfunction, joint sound, malocclusion, and locking of the mouth. Conservative and invasive techniques can be used for the treatment of TMJ internal derangements. The objective of the present study was to examine the benefit of an intra-articular platelet-rich plasma (PRP) injection and to compare this with arthrocentesis. Methods: Twenty patients (female: male; 15: 5; age 26, 3 +/- 9.3 years) for a total of 32 joints with reducible anterior disc dislocation, as confirmed by Magnetic Resonance Imaging (MRI), were divided into two groups. PRP was used for the study group, and arthrocentesis was used for the control group. Pain intensity, maximal interincisal opening, and TMJ sounds were assessed and compared for evaluation of treatment success. Results: There was a statistically significant reduction in pain intensity and joint sound and an increase in mouth opening in the study group when compared with the control group. Conclusions: This study shows that intra-articular PRP injection for the treatment of reducible disc displacement of the TMJ is a more effective method than arthrocentesis. (C) 2014 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.Öğe Investigation of Skin Graft Viability with Dmem and Ham's F-12 Fluids(LIPPINCOTT WILLIAMS & WILKINS, 2001) Tosun, Zekeriya; Aktan, Tahsin Murad; Hoşnuter, Mübin; Savacı, Nedim; Duman, Selçuk[Abstract not Available]Öğe Nitric oxide synthase inhibition prevents intestinal damage in gastroschisis: A morphological evaluation in chick embryos(W B SAUNDERS CO, 1999) Dilsiz, Alaeddin; Gündoğan, Ahmet H.; Aktan, Tahsin Murad; Duman, Selçuk; Aktuğ, TanjuPurpose: Increased small bowel nitric oxide (NO) synthase activity accused for postnatal intestinal dysmotility in gastroschisis. The purpose of this study is to evaluate the effect of prenatal NO synthase inhibition on intestinal damage in gastroschisis. Methods: Sixteen-day-old fertilized chick eggs were divided into 4 groups. In the control group, the allantoic and amniotic membranes were opened to create a common cavity. In the gastroschisis group, a defect in the abdominal wall was made, and intestinal loops were exteriorized. In the gastroschisis pretreated with L-NAME group, gastroschisis was created, and L-NAME was administered into the amnioallantoic cavity for 4 days. In the gastroschisis sham pretreated group, after the same surgical procedure as the previous group, same amount of saline was given beside L-NAME. At the end of 20th day of incubation, intestinal morphological changes were investigated macroscopically and microscopically. Results:Macroscopic changes such as shortening, thickening, and fibrous adhesions were found in the exteriorized bowels of the just gastroschisis group and the gastroschisis pretreated saline group. However, there was only mild thickening in the gastroschisis pretreated with L-NAME group. Microscopically, compared with the gastroschisis group, serosal thickness, muscular thickness, and bowel wall thickness were found to be significantly lower in the gastroschisis pretreated with L-NAME group (128.0 +/- 19.3 pm and 239.5 +/- 3.0 mu m V57.0 +/- 8.2 mu m and 145.0 +/- 9.7 mu m). Conclusion: It is possible to decrease intrauterine intestinal morphological changes in gastroschisis by inhibiting NO synthase. Copyright (C) 1999 by W.B. Saunders Company.Öğe Treatment of Acute Vocal Fold Injury With Platelet-Rich Plasma(MOSBY-ELSEVIER, 2016) Cobden, Serap Bulut; Ozturk, Kayhan; Duman, Selcuk; Esen, Hasan; Aktan, Tahsin Murad; Avunduk, Mustafa Cihat; Elsurer, CagdasObjectives. Platelet-rich plasma (PRP) is a reliable and has low side-effect profile and has beneficial effects on wound healing. Its investigatory effects on wound-healing process were shown on various tissues. The aim of the present study was to evaluate effectiveness of PRP application on scar tissue of acute vocal fold injury. Materials andmethods. Twenty-fourWistar rats were used in the study. The entire layer of the lamina propria down to the thyroarytenoid muscle of 10 subjects was unilaterally injured by with a microscissor. Gelfoam-absorbed PRP was applied on the injured area for 10 minutes. Control group consisted of rats unilaterally injured using a microscissor, and gelfoam with normal saline was applied on the injured area. Following sacrifice, the larynxes were carefully dissected and removed for histopathologic examination. After excised larynx experiments, serial sections were prepared from vocal fold. Hematoxylin eosin and immunohistochemical staining were done for epithelial growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR1), and vascular endothelial growth factor (VEGF) staining for histopathologic examinations. Results. There was not a significant difference between the two groups for lymphocyte. Although collagen and VEGF were higher in the study group, there was not a significant difference between the groups (P > 0.05). There was a significant difference between control and study groups for EGFR and FGFR1(P < 0.05). Conclusions. PRP has beneficial effects on wound healing. PRP accelerates epithelization of injured rat vocal folds by inducing EGFR secretion. PRP is an autogenous, reliable, low side-effect profile, easily harvested material. PRP may be useful to prevent scar formation.Öğe Use of a laser to detect viable but immotile spermatozoa(BLACKWELL VERLAG GMBH, 2004) Aktan, Tahsin Murad; Montag, M; Duman, Selçuk; Görkemli, Hüseyin; Rink, Klaus; Yurdakul, TalatA prerequisite for the success of intracytoplasmic sperm injection (ICSI) is the use of viable sperm that will support oocyte activation and embryo development. Usually, spermatozoa are selected according to motility, which is a clear indication for viability. However, in cases with complete asthenozoospermia, it is impossible to select viable sperm by morphological means. Here we report for the first time on the use of a laser to discriminate between viable and dead immotile spermatozoa. The application of a single laser shot to the far end of the sperm tail causes a curling of the sperm tail only in viable sperm, similar to the reaction observed in the hypo-osmotic swelling test. Using this method for sperm selection prior to ICSI we achieved high fertilization and cleavage rates in cases with immotile sperm in fresh testicular biopsy material as well in cases with ejaculated immotile sperm. It is concluded that laser sperm interaction allows for the identification of viable but immotile spermatozoa.
