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Öğe The Adiponectin variants contribute to the genetic background of type 2 diabetes in Turkish population(ELSEVIER, 2014) Arikoglu, Hilal; Ozdemir, Hulya; Kaya, Dudu Erkoc; Ipekci, Suleyman Hilmi; Arslan, Ahmet; Kayis, Seyit Ali; Gonen, Mustafa SaitAdiponectin, an adipose tissue specific protein encoded by the Adiponectin gene, modulates insulin sensitivity and plays an important role in regulating energy homeostasis. Many studies have shown that single nucleotide polymorphisms (SNPs) in the Adiponectin gene are associated with low plasma Adiponectin levels, insulin resistance and an increased risk of type 2 diabetes mellitus. The aim of the present study was to evaluate the contribution of the Adiponectin gene polymorphisms in genetic background of type 2 diabetes in a Turkish population. In total, 169 unrelated and non-obese diabetic patients and 119 age- and BMI-matched nondiabetic individuals with no family history of diabetes were enrolled in this study. We detected a significant association between type 2 diabetes and two SNPs: SNP - 11391G>A. which is located in the promoter region of the Adiponectin gene, and SNP + 276G > T, which is found in intron 2 of the gene (P < 0.05). The silence SNP G15G ( + 45T > G) in exon 1 and SNP + 349A > G in intron 2 also showed a weak association with type 2 diabetes (P = 0.06 and P = 0.07, respectively), while SNPs - 3971A>G in intron 1 and Y111H, R112C and H241P in exon 3 showed no association (P > 0.05). In conclusion, these findings suggest that Adiponectin gene polymorphisms might be effective on susceptibility for type 2 diabetes development which emerged from the interactions between multiple genes, variants and environmental factors. (C) 2013 Elsevier B.V. All rights reserved.Öğe Association of Polymorphisms in TCF7L2 Gene with Gastric Cancer Risk: A Preliminary Study in Turkish(DERMAN MEDICAL PUBL, 2015) Kaya, Dudu Erkoc; Arikoglu, Hilal; Sumen, Incilay Celik; Avci, Ebru; Ata, Ozlen; Arslan, EmineAim: The Transcription factor 7-like 2 (TCF7L2) gene, located on chromosome 10q25.3, encodes a transcription factor, which contains a high mobility group box, demonstrated in association with many cancer types. This factor is a critical part of Wnt/beta-catenin signaling pathway that plays key roles in regulation of embryonic development and homeostasis in mature tissues. It is known that the constant activation of Wnt/beta-catenin signaling pathway can cause cancer development. In this study, it is aimed to reveal the association between rs7903146C>T and rs12255372G/T single nucleotide polymorphisms in TCF7L2 gene, the key effector of Wnt/beta-catenin signaling pathway, and gastric cancer and to determine associated risk alleles. Material and Method: In our study, polymorphisms in TCF7L2 gene were genotyped using PCR-PFLP technique in 38 patients with gastric cancer and 48 healthy individuals. The obtained data were statistically analyzed and p<0.06 was accepted significant in all assessments. Results: No significant association was determined between rs7903146C>T and disease (p> 0.05) while 122553720>T polymorphism was associated with the disease under additive model (OR: 0.366 195% Cl: 0.135-0.989] p=0.047). Discussion: This is the first study to examine the association between TCF7L2 gene and gastric cancer risk in Turkish population and suggests that rs12255372C>T could be a potential indicator for gastric cancer. On the other hand, further studies are required which will be carry out in more increased number of samples in a wider population.Öğe ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS)+276 AND+349 IN THE ADIPONECTIN GENE WITH TYPE 2 DIABETES MELLITUS IN TURKISH POPULATION(JOHN WILEY & SONS INC, 2009) Arikoglu, Hilal; Kaya, Dudu Erkoc; Ozdemir, Hulya; Gotten, Mustafa Sait; Ipckci, Suleyman; Arslan, Ahmet; Hepdogru, Melda Aksoy[Abstract not Available]Öğe The cytotoxic effects of Juglone on pancreatic cancer cells in presence of sodium selenite and sodium ascorbate(LIPPINCOTT WILLIAMS & WILKINS, 2015) Erkoc-Kaya, Dudu; Avci, Ebru; Asik, Aycan; Arikoglu, Hilal[Abstract not Available]Öğe Effect of Grape Seed Extract on ß-Catenin Gene Expression and Hyperglycemia in Rats Induced by Streptozotocin(Selçuk Üniversitesi, 2023 Haziran) Arslan, Emine; Arikoglu, Hilal; Mutlu, Elif GulbahceAmaç: Tip 2 Diabetes Mellitus, insülin direnci ve/veya insülin eksikliği veya yetersizliği durumunda yüksek kan şekeri ile karakterize metabolik bir hastalıktır. Wnt/?-katenin sinyal yolu, tüm fizyolojik süreçlerde rol oynar. Yoldaki herhangi bir kusur diyabetin gelişmesine neden olur. Bu çalışmanın amacı, deneysel Tip 2 diyabetik sıçanlarda üzüm çekirdeği ekstresinin (GSE) vücut ağırlığı, kan şekeri ve ?-katenin geni ekspresyonu üzerindeki etkisini belirlemektir. Gereç ve Yöntem: Sıçanlar Kontrol, Diyabetik Kontrol ve Tedavi grupları (100, 200, 400 mg/kg GSE) olmak üzere beş gruba ayrıldı. Diyabet ve tedavi gruplarındaki ratlara yüksek yağlı diyet ve düşük doz streptozotosin (35 mg/ kg) verildi. Deney süresince sıçanların vücut ağırlıkları haftalık olarak kaydedildi. Suda çözünen ekstraktlar dört hafta boyunca gavaj yoluyla uygulandı ve çalışmada tedavi sonunda hayvanlardan alınan karaciğer, pankreas ve kan örnekleri kullanıldı. Bulgular: GSE tedavisinin diyabete bağlı genel kilo kaybı üzerine etkisinin minimal olduğu, 100 ve 400 mg/kg GSE dozlarının kan şekerini düşürdüğü gözlendi (p<0,05). ?-katenin gen ekspresyonu sonuçlarına göre karaciğer dokusu (p>0,05) ve pankreas dokusu (p<0,05) açısından gruplar arasında istatistiksel olarak anlamlı fark yoktu. Öneri: Elde edilen bulgulara göre üzüm çekirdeği diyabetin tedavisinde umut verici olabilir.Öğe Effects of Single Nucleotide Polymorphisms in K-ATP Channel Genes on Type 2 Diabetes in a Turkish Population(ELSEVIER SCIENCE INC, 2012) Gonen, Mustafa Sait; Arikoglu, Hilal; Kaya, Dudu Erkoc; Ozdemir, Hulya; Ipekci, Suleyman Hilmi; Arslan, Ahmet; Kayis, Seyit AliBackground and Aims. ATP-sensitive potassium (K-ATP) channels of pancreatic beta-cells play a key role in glucose-stimulated insulin secretion mechanism. The Kir6.2 protein, forming the K-ATP channel pore inwardly, and the SUR1 protein that surrounds it forming the outside part of the channel were encoded by ABCC8 and KCNJ11 genes, respectively. Recent studies reported that the single nucleotide polymorphisms (SNPs) established in these genes are associated with defects in insulin secretion and type 2 diabetes mellitus (T2DM). We aimed to investigate the allele profiles and the risk alleles of the ABCC8 and KCNJ11 genes and to highlight the associations with the disease in patients in Konya region of Turkey where T2DM is common. Methods. In this study, 169 patients with T2DM and 119 healthy controls were included. A total of 29 SNPs in ABCC8 and KCNJ11 genes were screened by PCR-SSCP technique and sequenced. Biochemical parameters and genotype-phenotype relationships were analyzed using variance analysis. Results. R1273R silent substitution in exon 31 and 16/-3t -> c substitution in noncoding region of exon 16 of ABCC8 gene showed a significant association (OR 4.8 [95% CI 2.41-9.77], p < 0.001 and OR 3.5 [95% CI 1.64-7.40], p < 0.001 under dominant and recessive models, respectively). We detected a significant association between E/K heterozygote genotype and reduced plasma insulin level in patients with T2DM (p < 0.05). Conclusions. ABCC8 exons 16 and 31 variants increase susceptibility to T2DM and KCNJ11 E23K decreases insulin secretion in a Turkish population (C) 2012 MISS. Published by Elsevier Inc.Öğe Expression profile and polymorphisms of actin genes in protoscoleces of Echinococcus granulosus from sheep in central Turkey(ELSEVIER SCIENCE BV, 2009) Arikoglu, Hilal; Arslan, Ahmet; Hepdogru, Melda A.; Turhan, Ahmet B.The expression levels and polymorphisms of two actin genes, EgactI and EgactII, were investigated in the protoscoleces of Echinococcus granulosus isolated from sheep in the central region of Turkey. Only the EgactII gene was found to be expressed at detectable levels by protoscoleces in the present study. PCR-RFLP analysis and following sequence analysis revealed that a partial EgactI gene is more polymorphic than a partial EgactII gene. And, three alleles for EgactI gene were identified. In all samples, including three alleles, there were nucleotide substitutions at six positions of the partial EgactI gene, and were distinct from the reference sequence from GenBank (TM) database. These differences are referred to as the Anatolian haplotype. (C) 2009 Elsevier B.V. All rights reserved.Öğe Investigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cells(ELSEVIER SCIENCE BV, 2016) Avci, Ebru; Arikoglu, Hilal; Kaya, Dudu ErkocJuglone, a natural component, is shown to have cytotoxic and apoptotic effects in several cancer cell lines. However, little is known about its effects on invasion and metastasis. In this study, we aimed to determine the antimetastatic effect of juglone in the BxPC-3 and PANC-1 pancreatic cancer cell lines. Cytotoxic effect of juglone was evaluated by using MTT (3-(4,5-Dimethylthiazo1-2-yl)-2,5-Diphenyltetrazolium Bromide) test. The cells were treated with juglone atÖğe Investigation of Juglone's apoptotic effect on PANC-1 pancreatic cancer cells(LIPPINCOTT WILLIAMS & WILKINS, 2015) Asik, Aycan; Arikoglu, Hilal; Dursunoglu, Duygu; Menevse, Esma[Abstract not Available]Öğe Investigation of Juglone's effects on invasion and metastasis in BxPC-3 pancreatic cancer cells(LIPPINCOTT WILLIAMS & WILKINS, 2015) Avci, Ebru; Arikoglu, Hilal; Kaya, Dudu Erkoc[Abstract not Available]Öğe The potential of microarray databases to identify tissue specific genes(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2016) Kaplan, Selcuk; Boztepe, Saim; Arikoglu, HilalTissue specific genes play important roles in development and metabolism. Currently, GenBank has 3363628 GEO Profiles and 397988 microarray data related to various tissues. To evaluate the huge amounts of data and identify tissue specific genes, it is necessary to develop and use new strategies. To that end, this study discusses if microarray and microarray related GEO Profiles are a useful tool to identify new tissue specific genes. In the current study, adipose tissue sellected as a target tissue in order to find new tissue specific genes. Therefore, the human and mouse microarray data were analyzed comparatively. To support the microarray data, adipose tissue related GEO Profiles were selected from PubMed. Subsequently, adipose tissue related microarray and GEO Profiles were analysed simultaneously. According to analysis of microarray and GEO Profiles, Chrdl1 (Chordin-like 1) gene was hypothesized as a novel adipose specific gene. In order to test the hypothesis, RT-PCR analysis were performed for the bovine tissue distribution. As a result, the hypothesis was successfully tested and Chrdl1 gene was found highly specific in bovine adipose tissue than in various other tissues. Thus, it is concluded that microarray and microarray related GEO Profiles are a useful tool to identify new tissue specific genes.Öğe Transcription factor 7-like 2 (TCF7L2) gene polymorphisms are strong predictors of type 2 diabetes among nonobese diabetics in the Turkish population(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2017) Erkoc Kaya, Dudu; Arikoglu, Hilal; Kayis, Seyit Ali; Ozturk, Onur; Gonen, Mustafa SaitBackground/aim: Type 2 diabetes (T2D) is a multifactorial disease, determined by environmental and genetic factors. Currently, the transcription factor 7-like 2 (TCF7L2) gene shows the strongest association with T2D. In this study, we investigated whether TCF7L2 gene polymorphisms are associated with T2D in a Turkish population. Materials and methods: Using PCR-RFLP and PCR-SSCP, we genotyped six intronic polymorphisms in the TCF7L2 gene, commonly associated with T2D, in 169 individuals with diabetes and 119 healthy controls. Results: We found that rs7903146 C -> T substitution in intron 3 (OR: 1.9, P = 0.005) and rs12255372 G -> T substitution in intron 4 (OR: 2.1, P = 0.002) were significantly associated with T2D while other SNPs were not associated (P > 0.05). We determined no association between TCF7L2 gene polymorphisms and fasting glucose, fasting insulin, HbA1c, or HOMA-IR levels (P > 0.05), except for rs7903146 C -> T substitution, which was significantly associated with the fasting glucose level (P = 0.003). Conclusion: Our results indicate that, in the Turkish population, the T allele of the rs7903146 (C -> T) and rs12255372 (G -> T) polymorphisms in the TCF7L2 gene is an independent risk factor for the development of T2D.
