Effect of Grape Seed Extract on ß-Catenin Gene Expression and Hyperglycemia in Rats Induced by Streptozotocin
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Dosyalar
Tarih
2023 Haziran
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Tip 2 Diabetes Mellitus, insülin direnci ve/veya insülin eksikliği veya yetersizliği durumunda yüksek kan şekeri ile karakterize metabolik bir hastalıktır. Wnt/?-katenin sinyal yolu, tüm fizyolojik süreçlerde rol oynar. Yoldaki herhangi bir kusur diyabetin gelişmesine neden olur. Bu çalışmanın amacı, deneysel Tip 2 diyabetik sıçanlarda üzüm çekirdeği ekstresinin (GSE) vücut ağırlığı, kan şekeri ve ?-katenin geni ekspresyonu üzerindeki etkisini belirlemektir. Gereç ve Yöntem: Sıçanlar Kontrol, Diyabetik Kontrol ve Tedavi grupları (100, 200, 400 mg/kg GSE) olmak üzere beş gruba ayrıldı. Diyabet ve tedavi gruplarındaki ratlara yüksek yağlı diyet ve düşük doz streptozotosin (35 mg/ kg) verildi. Deney süresince sıçanların vücut ağırlıkları haftalık olarak kaydedildi. Suda çözünen ekstraktlar dört hafta boyunca gavaj yoluyla uygulandı ve çalışmada tedavi sonunda hayvanlardan alınan karaciğer, pankreas ve kan örnekleri kullanıldı. Bulgular: GSE tedavisinin diyabete bağlı genel kilo kaybı üzerine etkisinin minimal olduğu, 100 ve 400 mg/kg GSE dozlarının kan şekerini düşürdüğü gözlendi (p<0,05). ?-katenin gen ekspresyonu sonuçlarına göre karaciğer dokusu (p>0,05) ve pankreas dokusu (p<0,05) açısından gruplar arasında istatistiksel olarak anlamlı fark yoktu. Öneri: Elde edilen bulgulara göre üzüm çekirdeği diyabetin tedavisinde umut verici olabilir.
Aim: Type 2 Diabetes Mellitus is a metabolic disorder characterized by high blood sugar in case of insulin resistance and/or insulin deficiency or insufficiency. The Wnt/?-catenin signaling pathway plays a role in in all physiological processes. Any defect in the pathway causes diabetes to develop. The aim ofthis study was to determine the effect of grape seed extract(GSE) on body weight, blood glucose and expression of ?-catenin gene in experimental Type 2 diabetic rats. Materials and Methods: Rats were divided into five groups as Control, Diabetic Control and Treatment groups (100, 200, 400 mg/kg GSE). The rats in the diabetes and treatment groups were fed with high-fat diet and were administered low dose of streptozotocin (35 mg/kg). Body weights of the rats were recorded weekly during the experiment. The water-soluble extracts were administered by gavage for four weeks and liver, pancreas and blood samples taken from animals at the end of treatment were used in the study. Results: It was observed that the effect of GSE treatment on overall weight loss due to diabetes was minimal, and 100 and 400 mg/kg GSE doses lowered blood sugar (p<0.05). According to the results of ?-catenin gene expression, there was no statistically significant difference between the groups in liver tissue (p>0.05) and pancreatic tissue (p<0.05). Conclusion: According to the findings, grape seed may be promising in the treatment of diabetes.
Aim: Type 2 Diabetes Mellitus is a metabolic disorder characterized by high blood sugar in case of insulin resistance and/or insulin deficiency or insufficiency. The Wnt/?-catenin signaling pathway plays a role in in all physiological processes. Any defect in the pathway causes diabetes to develop. The aim ofthis study was to determine the effect of grape seed extract(GSE) on body weight, blood glucose and expression of ?-catenin gene in experimental Type 2 diabetic rats. Materials and Methods: Rats were divided into five groups as Control, Diabetic Control and Treatment groups (100, 200, 400 mg/kg GSE). The rats in the diabetes and treatment groups were fed with high-fat diet and were administered low dose of streptozotocin (35 mg/kg). Body weights of the rats were recorded weekly during the experiment. The water-soluble extracts were administered by gavage for four weeks and liver, pancreas and blood samples taken from animals at the end of treatment were used in the study. Results: It was observed that the effect of GSE treatment on overall weight loss due to diabetes was minimal, and 100 and 400 mg/kg GSE doses lowered blood sugar (p<0.05). According to the results of ?-catenin gene expression, there was no statistically significant difference between the groups in liver tissue (p>0.05) and pancreatic tissue (p<0.05). Conclusion: According to the findings, grape seed may be promising in the treatment of diabetes.
Açıklama
Anahtar Kelimeler
β-katenin, tip 2 diabetes mellitus, gen ifadesi, streptozotosin, üzüm çekirdeği ekstresi, β-catenin, type 2 diabetes mellitus, gene expression, streptozotocin, grape seed extract
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
19
Sayı
2
Künye
Mutlu, E. G., Arslan, E., Arikoglu, H., (2023). Effect of Grape Seed Extract on ß-Catenin Gene Expression and Hyperglycemia in Rats Induced by Streptozotocin. Eurasian Journal of Veterinary Sciences, 39(2), 68-76.
DOI: 10.15312/EurasianJVetSci.2023.400
