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Öğe Blood and Colostrum/Milk Serum Gamma-Glutamyltransferase Activity as a Predictor of Passive Transfer Status in Lambs(Blackwell Verlag Gmbh, 2003) Maden, Mehmet; Altunok, Vahdettin; Birdane, Fatih Mehmet; Aslan, V.; Nizamlıoğlu, MustafaThe importance of blood and colostrum/milk serum gamma-glutamyl transferase (gamma-GT) enzyme activity was evaluated to assess passive transfer status in healthy lambs. Thirty Akkaraman sheep (3-6 years old) were used which had normal pregnancy period and the same conditions, and the age of the lambs ranged between 0 and 15 days. Blood and colostrum/milk samples were collected from sheep and lambs after birth, before suckling (0) and after on 1st, 3rd, 7th and 15th days. Serum immunoglobulin G (IgG) concentration was determined by the use of Single Radial Immunodiffusion method. Serum gamma-GT activity was measured, using a commercially available kit in blood and colostrum/milk samples. Correlations were carried out between immunoglobulin and gamma-GT levels. Regression models (simple and multiple) were calculated with significant data. Linear correlation was determined between colostrum/milk gamma-GT activity and IgG concentrations and between serum gamma-GT activity and IgG concentrations in lambs on the 0 day. (r: 0.607, P: 0.001), 1st (r: 0.768, P: 0.001) and the 3rd (r: 0.603, P: 0.001) days and on the 1st (r: 0.637, P: 0.001) and 3rd (r: 0.478, P: 0.012) days in the experiment, respectively. Multivariate regression models were developed to estimate sample IgG concentration. Serum and colostrum/milk IgG concentration could be predicted using the formula: lamb serum IgG = 825 + 0.688 (lamb gamma-GT) + 52 (days); colostrum/milk IgG = 832 + 0.505 (colostrum/milk gamma-GT) - 167 (days). The regression models were moderately accurate in predicting serum IgG concentration (R-2 = 0.51) and colostrum/milk IgG concentration (R-2 = 0.55). Test sensitivity and positive predictive values for serum gamma -GT enzyme activity were found to be 96 and 100% and for colostrum/milk gamma-GT enzyme activity were found to be 100 and 68% to prediction IgG concentration. Serum and colostrum/milk gamma-GT activity can be used to assess passive transfer status of lambs. Along with this, regression models used to calculate serum and colostrum/milk gamma-GT activities found to be useful to estimate sample IgG concentration. The use of serum and colostrum/milk gamma-GT enzyme activity was found useful especially after birth on the 0, 1st and 3rd days.Öğe Clinical Efficacy of Florfenicol in the Treatment of Calf Respiratory Tract Infections(Royal Netherlands Veterinary Assoc, 2002) Aslan, V.; Maden, Mehmet; Erganis, Osman; Birdane, Fatih Mehmet; Çorlu, M.This paper reports on a study of the aetiology of calf pneumonia and the clinical efficacy of florfenicol, a new antibiotic in Turkey. Twenty-seven weaned and unweaned calves (13 males and 14 females) between I and 16 months of age brought to the clinics of Selcuk University, Faculty of Veterinary Science. Broncho-alveolar lavage (BAL) fluid samples were taken from the animals diagnosed to have upper respiratory tract infection associated with bronchitis (N=2), bronchitis (N=5), bronchopneumonia (N=4), pneumonia (N=3), pleuropneumonia (N=11), bronchopneumonia plus pulmonary oedema (N=2) based on the results of the clinical and laboratory examinations. Then microbiological isolation and antibiotic culturing were performed. The animals were treated with I ml/15 kg (20 mg/kg) florfenicol (Nuflor(R), DIF) twice within 48 hours via intramuscular injection. At the end of the treatment, 23 of the weaned and unweaned calves were completely healed, I calf had died and 3 calves showed no healing. The results of BAL samples and microbiological examinations of the 3 calves that did not respond to the treatment indicated that these cases were affected by mixed infections of yeasts, fungi, and bacteria. Widespread pleuropneumonia was observed. According to the results of the microbiological examination of the BAL samples, Mannheimia (Pasteurella) haemolytica had the highest isolation rate (25%) compared with the other isolated bacteria, namely, Klebsiella pneumonia (20%), Actinomyces pyogenes (15%), beta-hemolytic streptococci. (10%), Staphylococcus spp. (5%), and E. coli (5%). The study also revealed fungi [Penicillum spp. (5%) and Aspergillus spp. (5%)] and two calves (10%) had a yeast infection.. We conclude that florfenicol has a high bacteriological and clinical efficacy (100% and 96% respectively) in the treatment of calf respiratory tract diseases.Öğe Effect of Antibiotic Treatment of Young Calves on Glucose Absorption and Some Plasma Components(1989) Aslan, V.; Nizamlıoğlu, M.; Kalaycıoğlu, L.; Acet, A.Oral treatment of 10-15 day-old calves with either chloramphenicol (55 mg/kg body wt) or tetracycline (11 mg/kg body wt) did not induce diarrhoea. Treatment caused significant elevation of plasma Na+, K+ and total protein. Calves treated with chloramphenicol showed retarded glucose absorption from the gut. © 1989.Öğe EFFECT OF ANTIBIOTIC-TREATMENT OF YOUNG CALVES ON GLUCOSE-ABSORPTION AND SOME PLASMA COMPONENTS(BAILLIERE TINDALL, 1989) Aslan, V.; Nizamlıoğlu, M.; Kalaycıoğlu, L.; Acet, A.[Abstract not Available]
