Blood and Colostrum/Milk Serum Gamma-Glutamyltransferase Activity as a Predictor of Passive Transfer Status in Lambs
Yükleniyor...
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Blackwell Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The importance of blood and colostrum/milk serum gamma-glutamyl transferase (gamma-GT) enzyme activity was evaluated to assess passive transfer status in healthy lambs. Thirty Akkaraman sheep (3-6 years old) were used which had normal pregnancy period and the same conditions, and the age of the lambs ranged between 0 and 15 days. Blood and colostrum/milk samples were collected from sheep and lambs after birth, before suckling (0) and after on 1st, 3rd, 7th and 15th days. Serum immunoglobulin G (IgG) concentration was determined by the use of Single Radial Immunodiffusion method. Serum gamma-GT activity was measured, using a commercially available kit in blood and colostrum/milk samples. Correlations were carried out between immunoglobulin and gamma-GT levels. Regression models (simple and multiple) were calculated with significant data. Linear correlation was determined between colostrum/milk gamma-GT activity and IgG concentrations and between serum gamma-GT activity and IgG concentrations in lambs on the 0 day. (r: 0.607, P: 0.001), 1st (r: 0.768, P: 0.001) and the 3rd (r: 0.603, P: 0.001) days and on the 1st (r: 0.637, P: 0.001) and 3rd (r: 0.478, P: 0.012) days in the experiment, respectively. Multivariate regression models were developed to estimate sample IgG concentration. Serum and colostrum/milk IgG concentration could be predicted using the formula: lamb serum IgG = 825 + 0.688 (lamb gamma-GT) + 52 (days); colostrum/milk IgG = 832 + 0.505 (colostrum/milk gamma-GT) - 167 (days). The regression models were moderately accurate in predicting serum IgG concentration (R-2 = 0.51) and colostrum/milk IgG concentration (R-2 = 0.55). Test sensitivity and positive predictive values for serum gamma -GT enzyme activity were found to be 96 and 100% and for colostrum/milk gamma-GT enzyme activity were found to be 100 and 68% to prediction IgG concentration. Serum and colostrum/milk gamma-GT activity can be used to assess passive transfer status of lambs. Along with this, regression models used to calculate serum and colostrum/milk gamma-GT activities found to be useful to estimate sample IgG concentration. The use of serum and colostrum/milk gamma-GT enzyme activity was found useful especially after birth on the 0, 1st and 3rd days.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
50
Sayı
3
Künye
Maden, M., Altunok, V., Birdane, F. M., Aslan, V., Nizamlıoğlu, M., (2003). Blood and Colostrum/Milk Serum Gamma-Glutamyltransferase Activity as a Predictor of Passive Transfer Status in Lambs. Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 50(3), 128-131. Doi: 10.1046/j.1439-0450.2003.00629.x
