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Öğe C677T Polymorphism of the Methylenetetrahydrofolate Reductase Gene Does Not Affect Folic Acid, Vitamin B-12, and Homocysteine Serum Levels in Turkish Children with Neural Tube Defects(FUNPEC-EDITORA, 2010) Erdoğan, M. O.; Yıldız, S. H.; Solak, M.; Eser, O.; Coşar, E.; Eser, B.; Köken, R.; Büyükbaş, S.Association between neural tube defects (NTDs) and C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene was suspected, because the MTHFR gene codes for a key enzyme in folate metabolism. Its deficiency usually leads to significant reductions in plasma concentrations of folate, vitamin B-12 and methionine, whereas homocysteine levels are increased. We examined folate, vitamin B-12 and homocysteine serum concentrations and polymorphism of the C677T MTHFR gene in Turkish children with neural tube defects. Thirty-three children with NTDs, 26 mothers and 48 healthy individuals were studied. C677T MTHFR polymorphism was determined by melting curve analyses (LightCycler (R)). The levels of folate, vitamin B-12 and homocysteine serum concentrations in NTDs were evaluated and compared, along with information concerning alleles of the MTHFR gene. C677T allele frequencies in NTD children and their mothers were similar to those found in controls. Serum folate and vitamin B-12 concentrations were significantly higher in NTD children than that of controls. Serum homocysteine concentrations were not significantly higher in NTD children and mothers. We concluded that C677T MTHFR gene polymorphism does not affect folic acid, vitamin B-12 and homocysteine metabolism in Turkish children with NTDs. C677T polymorphism of the MTHFR gene cannot be regarded as a major risk factor for NTDs in Turkish children.Öğe Cytotoxic Effects of Dental Desensitizers on Human Gingival Fibroblasts(Wiley-Liss, 2006) Şengün, A.; Büyükbaş, S.; Hakkı, S. S.The purpose of this study was to evaluate the effects of three different desensitizers on the cell viability and morphology of human gingival fibroblasts (HGF). Human gingival tissues were obtained from individuals who have clinically, healthy periodontium. HGF were grown at 37 degrees C in humidified atmosphere of 5% CO2 in Dulbecco's modified eagle's medium, supplemented with glutamine, penicillin, streptomycin, and 10% fetal bovine serum. The cells were treated with different concentrations (0.1, 0.3, and 0.5 mu L/mL) of desensitizers (Gluma Desensitizer, Seal&Protect, and MicroPrime). After 24- and 48-h exposure to the desensitizer solutions, the viable cells were examined using a hemocytometer. To monitor HGF viability, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was used and cell morphology was also observed at 48 h. Following exposure to concentrations of 0.1 mu L/mL of test materials for 24 h, cell survival rates for Gluma Desensitizer (106%) and Micro Prime (62%) were not significantly different from the control, while it was significant for Seal&Protect (50%). Growing cells were significantly inhibited by all tested materials for 48 h (p < 0.05) in survival rates of 51, 47, and 31%, respectively. On the basis of the MTT assay, the cytotoxic effect of MicroPrime was more prominent, especially at high concentrations, than does Gluma Desensitizer and Seal&Protect. After exposure to Seal&Protect and MicroPrime, HGF became retracted, rounded in appearance and had loss of normal organization, leading to enlargement of intercellular space when compared with Gluma Desensitizer. As a conclusion, taking the limitations of an in vitro experiment into consideration, the cytotoxic effects were varied, depending on the chemical composition and exposure periods of the tested desensitizers.Öğe Effect of Cigarette Smoking on High Density Lipoprotein Cholesterol Elevation(2009) Yazar, Hayrullah; Büyükbaş, S.The effects of cigarette smoking which is either active or passive still is in agenda by clinicians on lipid levels specifically high density lipoprotein (HDL)-cholesterol in blood. The represented study has been performed on 1006 adult persons with 598 women and 408 men between age group of 25-55 from the Cumra Government Hospital. Blood samples taken from 12 h fasting between 8 and 9.30 AM from all the patients were saved into tubes. After coagulation, HDL-cholesterol analysis was completed by separation serums. The average HDL-choles- terol levels of 1006 adult persons is found as 50.67 ± 12.30 mg/dl. HDL-cholesterol levels of men is 48.11 ± 12.59 mg/dl and for women is 52.41 ± 11.80 mg/dl. In this study, it was aimed to investigate the effect of active and passive cigarette smoking on blood HDL cholesterol levels.Öğe Effect of Snoring and Smoking on Blood Lipid Levels(2009) Yazar, Hayrullah; Tiftik, A. M.; Büyükbaş, S.This study reports the relationship with snore (obstructive sleep apnea syndrome, OSAS), cigarette smoking and blood lipid levels in human. This work carried out on adult patients (age: 25-55) who applied to the polyclinic of Cumra Public Hospital between January 2005 and November 2006, of 598 women and 408 men. As a result, it has been found that the snoring and cigarette smoking adversely effect on blood lipid levels.
