Yazar "Basarali, Kemal" seçeneğine göre listele
Listeleniyor 1 - 7 / 7
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe The effect of theophylline on oxidative stress in serum and tissues in mechanical ventilation-induced diaphragm dysfunction(TURKISH BIOCHEM SOC, 2011) Uzun, Kursat; Aydin, Nihal Bakirkalay; Teke, Turgut; Buyukbas, Sadik; Basarali, KemalIntroduction: The role of oxidative stress in mechanical ventilation induced diaphragm dysfunction (VIDD) was shown in various studies. In this animal model, we planned to evaluate the role of diaphragmatic and systemic stress on VIDD and the effect of theophylline application on the oxidative stress. Material and methods: Thirty healthy male Sprague-Dawley rats were used. They were evenly divided into 3 groups as Group 1, control group (no application); group 2, placebo group receiving mechanical ventilation (MV); Group 3, theophylline group receiving both MV and theophylline. Malondialdehyde (MDA) and nitric oxide (NO) levels with xanthine oxidase (XO) and superoxide dismutase (SOD) activities were measured in all groups to evaluate the oxidative stress at diaphragm, lung tissue and plasma. Results: SOD (0.01-0.07 U/mg) and XO (0.61-1.76 U/g) activities with NO (0.05-0.14 mu mol/g) levels at diaphragm; XO (0.36-2.26 U/g) activity at lungs and SOD (2.06-5.24 U/mL) and XO (7.76-14.04 U/mL) activities with MDA (0.35-1.09 nmol/L) levels at plasma were significantly increased in placebo group (p < 0.05) compared to control. Upon theophylline therapy, SOD (0.096 U/mg) activity at diaphragm and plasma NO (22.3-29.7 mu mol/L) levels were significantly increased (p < 0.05); whereas XO (0.39 U/g) activity at lungs and plasma MDA (0.36 nmol/L) levels were significantly decreased (p < 0.001) compared to placebo group. Conclusion: In this study, we showed that pulmonary and systemic oxidative stresses accompany MV application induced diaphragmatic oxidative stress in rats and this oxidative stress is predominantly occurs through XO pathway. Also it was demonstrated that theophylline alleviates this oxidative stress by increasing the SOD activity and repressing the XO activity. However, more animal and human studies at molecular level are needed on this subject. Conflict of Interest: Authors have no conflict of interest.Öğe The Effects of Caffeic Acid Phenethyl Ester and Ellagic Acid on the Levels of Malondialdehyde, Reduced Glutathione and Nitric Oxide in the Lung, Liver and Kidney Tissues in Acute Diazinon Toxicity in Rats(MEDWELL ONLINE, 2011) Alp, Harun; Aytekin, Ismail; Atakisi, Onur; Hatipoglu, Namik Kemal; Basarali, Kemal; Ogun, Metin; Buyukbas, SadikThe aim of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) and Ellagic Acid (EA) on acitivities of Malondialdehyde (MDA), reduced Glutathione (GSH) and Nitric Oxide (NO) in rat lung, liver and kidney tissues in acute Diazinon (DI) toxicity. Six groups of 6 Sprague Dawley rats were used comprising control, CAPE, EA, DI control, DI+CAPE and DI+EA. Tissue samples were analysed for GSH, MDA and NO levels in lung, liver and kidney tissues. Biochemical parameters were measured colormetrically by spectrophotometer. Control, CAPE and EA groups showed no statistically significant difference whereas DI+medication groups revealed that CAPE and EA increased the level of GSH in liver tissue by blocking the DI effect. NO levels in lung, liver and kidney tissues were significantly increased by DI but CAPE and EA attenuated those levels. In DI+medication groups, MDA levels showed no significant change in kidney and liver tissues but in lung tissues, CAPE and EA reduced the MDA level by blocking the DI effect. It was concluded that CAPE and EA which showed similar effects to each other could be used for protection and support against oxidative stress caused by acute DI intoxication.Öğe Effects of caffeic acid phenethyl ester, ellagic acid, sulforaphane and curcumin on diazinon induced damage to the lungs, liver and kidneys in an acute toxicity rat model(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2011) Alp, Harun; Aytekin, Ismail; Esen, Hasan; Basarali, Kemal; Kul, SevalThe aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), ellagic acid (EA), sulforaphane (SFN) and curcumin (CUR) against the toxic effects of diazinon (DI). Sixty Sprague Dawley rats were randomly divided into 10 groups. Five groups were allocated as control groups comprising unmedicated control, CAPE, EA, SFN and CUR control groups. The remaining five groups were the study groups comprising DI, DI + CAPE, DI + EA, DI + SFN, and DI + CUR groups. The animals were sacrified 24 h after drug administrations. DI caused a decrease in acetyl cholinesterase (AChE) activity (P<0.05) and increases in gamma-glutamyltransferase (GGT) and amylase activities. It also damaged the kidney, liver, and lung tissues. The negative effects of DI on these enzymes were confirmed histopathologically. Also, CAPE, EA, SFN and CUR reduced amylase and GGT activities and caused an increase in the AChE activities that were increased due to the toxic effects of DI. Thus, it was determined biochemically and histopathologically that these medication reduced the degenerative toxic effects created by DI in the lung, liver and kidney tissues. These findings led us to believe that CAPE, EA, SFN and CUR may be used as protective medicines in acute DI intoxication.Öğe Effects of grape seed extract supplementation on exercise-induced oxidative stress in rats(CAMBRIDGE UNIV PRESS, 2012) Belviranli, Muaz; Gokbel, Hakki; Okudan, Nilsel; Basarali, KemalThe aim of the present study was to investigate the effects of grape seed extract (GSE) supplementation on exercise performance and oxidative stress in acutely and chronically exercised rats. A total of sixty-four male rats were used in the study. Rats were divided into six groups: control, chronic exercise control, acute exercise control (AEC), GSE-supplemented control, GSE-supplemented chronic exercise and GSE-supplemented acute exercise groups. Chronic exercise consisted of treadmill running at 25 m/min, 45 min/d, 5 d a week for 6 weeks. Rats in the acute exercise groups were run on the treadmill at 30 m/min until exhaustion. GSE were given at 100 mg/kg of body weight with drinking water for 6 weeks. Plasma was separated from blood samples for the analysis of oxidative stress markers. There was no significant difference in time of exhaustion between the acute exercise groups. Plasma malondialdehyde (MDA) levels were higher in the acute exercise groups and lower in the chronic exercise groups. GSE supplementation decreased MDA levels. Xanthine oxidase and adenosine deaminase activities were higher in the AEC group compared to all the other groups. NO levels were increased with both chronic exercise and GSE supplementation. Superoxide dismutase and glutathione peroxidase activities were lower in the acute exercised groups and higher in the chronic exercised groups. GSE supplementation caused an increase in antioxidant enzyme activities. In conclusion, GSE supplementation prevents exercise-induced oxidative stress by preventing lipid peroxidation and increasing antioxidant enzyme activities.Öğe The protective effect of alpha-lipoic acid in experimental ovarian ischaemia-reperfusion injury(BLACKWELL PUBLISHING, 2007) Cosar, Emine; Sahin, Figen Kir; Koken, Guelenguel; Toy, Hatice; Basarali, Kemal; Buyukbas, SadikWe investigated the effect of alpha-lipoic acid (LA) on reperfusion injury in a rat ovarian torsion-detorsion model. The changes in tissue and plasma levels of malondialdehyde (MDA), end-product of lipid peroxidation, superoxide dismutase (SOD), xanthine oxidase (XO) and nitric oxide (NO), were determined. Ovarian histopathological findings were scored and compared among groups. Thirty-two female Sprague-Dawley rats were divided into four groups. Sham operation was performed in group I; in group II only ovarian torsion was performed. Group III received intraperitoneal injections of saline, and group IV received LA via intraperitoneal injections (LA group: aqueous solution at 36 mg/kg of body weight per day, saline group: equal volume of saline) 21, nine, and one hour before torsion of the ovary. Rats in the torsion group were killed after 360 degrees clockwise adnexial torsion for three hours, and ovaries were harvested. After three hours of adnexial detorsion, the rats in saline group and LA group were killed and adnexa were surgically removed. Ovarian tissue damage scores were significantly different among groups and were seen to correlate with tissue MDA levels. Ovarian tissue and serum MDA, NO and serum XO levels in the group III were significantly higher than those of the groups I and IV (P < 0.05). The serum levels of SOD in the group III were significantly lower than those of the groups I and IV (P < 0.05). These results suggest that LA pretreatment has beneficial effects in the prevention of ischaemia-reperfusion injury of the ovaries.Öğe Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary(WILEY, 2008) Sahin, Figen Kir; Cosar, Emine; Koken, Gulengul; Toy, Hatice; Basarali, Kemal; Buyukbas, SadikAim: We investigated the effects of aprotinin on reperfusion injury in a controlled experimental rat torsion-detorsion model. Methods: Thirty-two female Sprague-Dawley rats were divided into four groups. Sham operation was performed in group I; in group II only ovarian torsion was performed. In group III, torsion-detorsion was performed, plus 3 mL/kg saline was injected i.v. 30 min before detorsion. In group IV, torsion-detorsion was performed, plus aprotinin (30 000 KIU/kg) was injected 30 min before detorsion. Rats in the torsion group were killed after 360 degrees clockwise adnexial torsion for 3 h, and the ovaries were harvested. After 3 h of adnexial detorsion, the rats in the saline and aprotinin groups were killed and the adnexa were surgically removed. Results: Ovarian tissue damage scores were significantly different among groups. Ovarian tissue and serum malondialdehyde levels in group III were significantly higher than those of groups I and IV (P < 0.05). The serum levels of superoxide dismutase in group III were significantly lower than those of groups I and IV (P = 0.01). Tissue and serum xanthine oxidase, nitric oxide, and tissue superoxide dismutase levels were comparable among groups (P > 0.05). Conclusions: Aprotinin attenuates ischemia-reperfusion injury in a rat adnexial torsion-detorsion model.Öğe The relationship of metabolic syndrome, serum leptin levels and treatment in bipolar disorder(KURE ILETISIM GRUBU A S, 2008) Yumru, Mehmet; Savas, Esen; Gergerlioglu, H. Serdar; Basarali, Kemal; Kalenderoglu, Aysun; Savas, Haluk A.; Bueyuekbas, SadikObjective: In this study we aimed to evaluate the relationship between serum leptin. levels, metabolic syndrome and treatment in bipolar patients. Method: Sixty bipolar I disorder (BD) patients were enrolled; 29 patients were on mood stabilizer (MS) (lithium, valproic acid, carbamazepin, and lamotrigine) treatment, 31 patients were on atypical antipsychotics (AA) (risperidone, quetiapine, and olanzapine) for at least 3 months. Metabolic syndrome (MetS) was evaluated according to NCEP ATP III (Adult Treatment Protocol of the National Cholesterol Education Programme) criteria. Results: 36.7% of the patients were recognized as MetS. The AA subgroup had significantly more MetS than MS. Abdominal obesity was significantly correlated with leptin levels in BD. No significant association was found between MetS and leptin levels in BD. Discussion: AA taking bipolar patients had higher MetS rates. However, there was no significant difference among AAs for inducing MetS. Leptin levels were significantly correlated with abdominal obesity in bipolar patients but seem not to be associated with MetS.
