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Öğe Bacterial Colonization and Yeast Carriage on the Hands of Nurses(W B SAUNDERS CO LTD, 1996) Fındık, D.; Ural, O.; Baysal, B.The spread of pathogens from the environment to patients and from patient to patient frequently begins with the hands of hospital staff. Hands are in constant contact with the environment, and because of this reflect exposure to transient contaminants.‘-3 Candida species, particularly Candida albicans, are potential pathogens capable of causing both local and systemic infection in hospitalized patients. The development of invasive candidiasis is usually attributed to autoinfection after colonization, especially of the gastrointestinal tract. However, there is evidence that patient colonization and candidaemia can also originate from the hands of healthcare workers. We designed the present study to examine the bacterial colonization and the frequency of yeast carriage on the hands of the nurses working in separate units of the hospital of Faculty of Medicine of Selcuk University.Öğe Comparison of the Detection of Influenza A and B Viruses by Different Methods(SAGE PUBLICATIONS LTD, 2012) Ozdemir, M.; Yavru, S.; Baysal, B.OBJECTIVE: To investigate the detection of influenza viruses by three different methods. METHODS: Nasopharyngeal swabs were collected from patients with influenza symptoms and examined for influenza A and B viruses using a rapid antigen test, a multiplex polymerase chain reaction (PCR) test and a shell vial cell culture test. RESULTS: Using the shell vial cell culture test, the rapid antigen test and the multiplex PCR test in 130 patients, 31 (23.8%), 24 (18.5%) and 24 (18.5%) samples, respectively, were positive for influenza A and 10 (7.7%), nine (6.9%) and four (3.1%) samples, respectively, were positive for CHAIN REACTION TEST; influenza B. Compared with the shell vial test, the sensitivity, specificity, and positive and negative predictive values of the rapid antigen test were 77.4%, 93.3%, 80.0% and 93.1%, respectively, for influenza A, and 90.0%, 95.8%, 64.2% and 99.1%, respectively, for influenza B. The corresponding values for the multiplex PCR test were 77.4%, 95.9%, 85.7% and 93.1%, respectively, for influenza A, and 40.0%, 97.5%, 57.1% and 95.1%, respectively, for influenza B. CONCLUSIONS: The multiplex PCR test and the rapid antigen test are both effective in the detection of influenza A and B viruses.Öğe A Comperative Study on Allergic Reactions Related to Various Anaesthetic Agents(1997) Altındiş, M.; Tuncer, S.; Özmen, S.; Ökeşli, S.; Sütçü, A.; Baysal, B.This study was designed to determine and compare allergic reactions due to IV anaesthetic agents used during surgical operations. From the Departments of general surgery, urology and orthopaedics 60 adults patients (27 male and 33 female with 35.9±12.5 mean age) having no tumoral-endocrine pathology or allergy/athopy history and being classified as ASA I and II were included to the study. They were divided into four groups as equal in number. The groups were similar demographically. After permission of the hospital's ethic comittee, the 4 groups were respectively infused with propofol (2.5 mg/kg) thiopentone (5 mg/kg), etomidate (0.3 mg/kg) and midazolam (0.3 mg/kg) for induction. Serum IgE levels (by ELISA using EIA-Bioclone, Australia) and eosinophil and basophil counts (by using autoanalyser) were measured in blood samples drawn both just before, after induction and 24 hrs later. As a result, we detected thiopentone to cause significant increase in serum IgE levels and eosinophil and basophil counts more than those of the other 3 agents (p<0.05). We concluded that, instead of using thiopentone, propofol, etomidate or midazolam may be a safer alternative for patients with an allergy/athopy history.Öğe The Effect of Anti-HCV Seropositivity on the Tnf-? Level in Hemodialysis Patients [1](1995) Şengil, A. Z.; Gürbilek, M.; Altındiş, M.; Uysal, H.; Koşar, A.; Baysal, B.[Abstract not Available]Öğe Effects on Wound Healing of Different Incision Instruments: Experimental Study(2012) Şahin, Mustafa; Baysal, B.; Yılmaz, Hüseyin; Alptekin, Hüsnü; Uğraş, Serdar; Mehmetoğlu, İdrisObjective. Wound healing effects of scalpel, electrocautery, peak plasmablade, which are used for skin incisions and intestinal incisions, were searched. Material and method. 36 wistar albino rats 10-week were used as experimental animals. 3 groups were created. There were 12 wistar albino rats in each groups. Laparotomy by scalpel and transection to ascending colon were applied in the group I. The segment, which was transected, was anastomosised at once. The same processes were applied by electrocautery in the group II. The same processes were applied by peak plasmablade in the group III. Experimental animals were sacrified at the 7th post-operative day. 1 ml blood was taken by cardiac puncture for MDA measurement and then relaparotomy was applied. Approximately 4 cm intestinal segment, which included anastomosis line, was resected. Then anastomotic bursting pressures were measured. 4x4 cm2 abdominal wall was removed to measure rupture breaking pressure of incision. Tissue samples were got for incision line's and anastomosis line's histopathological examinations. Results. In the group II, anastomotic bursting pressure and rupture breaking pressure of abdominal wall were found less than the groups I and III. The difference between the groups was statistically reasonable (p<0.05). MDA values of each groups weren't statistically different. At anastomosis and incision line, any reasonable difference was found in terms of 7 criterions, which were evaluated histopathologically. Conclusion. We think that scalpel and peak plasmablade can be used for incisions safely. Peak plasmablade supplies hemostasis. So peak plasmablade shortens operation duration.Öğe Evaluation and Comparison of Three Different Anti-hepatitis C Virus Antibody Tests based on Chemiluminescence and Enzyme-linked Immunosorbent Assay Methods used in the Diagnosis of Hepatitis C Infections in Turkey(SAGE PUBLICATIONS LTD, 2009) Kesli, R.; Ozdemir, M.; Kurtoglu, M. G.; Baykan, M.; Baysal, B.The routine diagnosis of hepatitis C virus (HCV) infection is based on the detection of anti-HCV antibodies by two main methods (enzyme immunoassay [EIA] and chemiluminescence immunoassay [CIA]) but false-positives are a problem. We investigated three anti-HCV tests: two CIAs (Cobas (R) e 601 and Architects i2000SR); and one EIA (Ortho (R) HCV 3.0). Two other anti-HCV tests were also performed as supplementary and confirmatory tests, respectively: a recombinant strip immunoblot assay (RIBA HCV 3.0 SIA) and a reverse transcriptase polymerase chain reaction-based assay for HCV-RNA. After discriminating the false-positive results, the true anti-HCV seropositivity rate in 7156 serum samples was 0.91%. The seropositivity and false-positive rates for the Cobas (R) e 601, Architects i2000SR and Ortho (R) HCV 3.0 anti-HCV tests were 1.9% and 0.99%, 1.2% and 0.29%, and 0.87% and 0.01%, respectively. The mean level of HCV-RNA was 3399 x 10(3) IU/ml. Critical levels for false-positivity for HCV-RNA were a cut-off index of 200 for Cobas (R) e 601, a signal/cut-off (S/CO) of 5 for Architects i2000SR and an S/CO of 1.2 for Ortho (R) HCV 3.0. Positive and negative results for the RIBA HCV 3.0 SIA assay all accorded with the HCV-RNA assay, except for 23 (17%) 'indeterminate' results, all of which were negative with the HCV-RNA assay. In conclusion, to eliminate doubts related to false-positive findings in the initial HCV screening tests, additional confirmatory HCV-RNA assay should be performed.Öğe Investigation of Penicillin Tolerance and Erythromycin Resistance in Group A-Beta Hemolytic Streptococci Isolated From Throat Cultures(1998) Kaloğlu, G.; Tuncer, I.; Baysal, B.In this study, the penicillin tolerance and erythromycin resistance of 100 strains of group A beta hemolytic streptococci, isolated from throat cultures of patients who admitted to outpatient clinics of Selcuk University Medical School, were investigated by microdilution method. If the ratio of Minimal Bactericidal Concentration to Minimal Inhibitory Concentration (MBC/MIC) were higher than 32 ?g/ml, the strains have been evaluated as penicillin tolerant, if the MIC was equal and/or higher than 8 ?g/ml, the strains have been evaluated as erythromycin resistant. As a result, none of the strains was found as penicillin tolerant, whereas 4% of strains were found as erythromycin resistant.Öğe Retrospective Investigation of Specific Ige Antibody in Patients Who Have Allergic Semptoms in Konya Region(2009) Doğan, M.; Özdemir, M.; Baysal, B.In this study, it is aimed to investigate retrospectively the serum specific IgE antibodies in the patients who have allergic symptoms in Konya region. 1423 patients' serum were included in the study between the dates of June, 2004-July, 2005. 23867 specific IgE tests had been studied with commercial kits named REAST (Dr. Fooke Laboratorien GmbH, Neuss, Germany) and/or UniCAF System FEIA (Pharmacia Diagnostics, Sweden) which could measure the IgE antibody specific to allergen. In the study, the specific IgE positivity against inhalation panel allergen was found in the highest rate with 21.6 %. Other mix IgE measurements sequentially; the panel of food in the rate of 17.4 %, wild grass in the rate of 13.6 %, home dust panel in the rate of 12.1 %, tree panel in the rate of 8.8 %, animal epithelium panel in the rate of 5.5 %, mold panel in the rate of 5.4 % were found positive. In the specific IgE measurements; wasp in the rate of 25.8 %, big couch grass in the rate of 23.6 %, banana in the rate of 20.7 %, timothy in the rate of 20.5 %, wheat flour in the rate of 18.8 %, cockroach in the rate of 17.7 %, latex in the rate of 15.8 %, milk in the rate of 14 % were evaluated positive and IgE measurements against parakeet, cocoa, Penicillium notatum, Cladosporum herbarum, Aletnaria alternata were found approximately in the rate of 1 %. However these results reflect the allergy profile of Konya region, we are of the opinion that, it will be more useful to fix the atmosphere allergens in Konya region and to reprepare the allergy test panels. Also it is necessary to investigate allergen related with age group and systems which is effected.
