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Öğe Comparison of two specific Ig E test in detection of allergen in asthma disease with referance of prick test(2008) Özdemir M.; Kalem F.; Girgiç M.; Kalem A.C.; Baysal B.Objective: It is aimed to compare two specific methods of allergen tests in patients who have diagnosed as asthma with history, physical examination and breath function test and to evaluate the results by comparison with prick test as a standart in this study. Method: In this study serum samples had taken from 52 asthmatic patients. Using both spesific IgE RAST and UniCAP System FEIA; the specific IgE titres have been measured. Then the results have been compared statistically. Results: Sixty one parameters were positive with prick test. RAST method showed positive results for 46 parameters. 23 parameters were positive with UniCAP System. Conclusion: These results showed that RAST method shows better correlation with prick test than UniCAP System. In asthmatic patients; skin prick test and measurement of specific IgE levels are useful for detection of allergen. In asthmatic patients; if skin prick test and spesifik IgE levels gave similiar results, it could be possible to say that this allergen is the agent of allergic disease.Öğe Investigation of bacterial counts in air at intensive care units and operating rooms [Yo?un bakim üniteleri? ve ameli?yathanelerde havadaki? bakteri?yel parti?kül sayilarinin araştirilmasi](2010) Özdemir M.; Gündem N.S.; Baysal B.Nosocomial infection is an important problem in all modern hospitals. Hospital operating rooms (OR) and intensive care units (ICU) are the workplaces that need the highest hygiene standards. This study was conducted in intensive care units, operating rooms and neonatal intensive care units of Meram Medical Faculty Hospital of Selcuk University. Air Ideal machine was used for sampling the air and aspirates air through a perforated plate, which results in impaction of particles from an air stream onto the surface of agar medium. Thus, all of samples from different clinics were inoculated into blood agar and incubated aerobically 24 hours at 37°C. The isolated microorganisms were coagulase negative staphylococci, non-fermentative gram negatives and spore forming rods. On average counts of particle were 8160 cfu/m3 bacteria in neonatal intensive care units, 610 cfu/m3 bacteria in reanimation intensive care units, 2308 cfu/m3 bacteria in general surgery intensive care units, 2491 cfu/m3 bacteria in operating rooms. It is useful to determine the particle number in critical area in hospital in addition other infection prevention procedure.Öğe The investigation of bordatella antibody in different age group in Konya region [Konya bölgesinde de?işik yaş gruplarinda bu?maca antikorlarinin araştirilmasi](2006) Ülker Z.; Özdemir M.; Baysal B.Bodetella which is an infection caused by B.pertussis may cause epidemic disease. These infectious agents cause fatal disease in the case of inefficient antibody level. Immunization is life saving in these disease outbreaks. In order to investigate the effectiveness of bordetella immunisation B.pertussis antibody levels were investigated. The study group was consisted of 184 healthy subjects aged from 1 to 40 years. Serum samples were collected between August 2003 and October 2004. Serological assays for B.pertussis were performed with a standardized ELISA test; results were expressed in IU/ml. No significant difference between seroprevalance in B.pertussis, related with region and sex was found. Seropositivity for B.pertussis was increased by older age. It was concluded that this situation was related with Bordetella Infections in old age.Öğe The investigation of diphteria antibody in different age groups by ELISA method in Konya region [Konya bölgesinde farkli yaş gruplarinda difteri antikorlarinin ELISA yöntemi ile araştirilmasi](2006) Ülker Z.; Özdemir M.; Baysal B.Infant death is caused mostly by infectious diseases which can be prevented with an effective immunization programme. C.diphtheriae may cause epidemic outbreak in the population. This infectious agent cause fatal disease in the case of unprotective antibody level. Immunization is life saving in these disease outbreaks. In this study we aimed to investigate the effectiveness and protectiveness of diphteria vaccination. For this purpose 184 healthy subjects aged from 1 to 40 years in Konya region were included in the study. Serum samples were collected between August 2003 and October 2004. Serological assays for C.diphtheriae were performed with a standardized ELISA method; results were expressed in IU/ml. In both pediatric and adult age groups, serum antibody levels were seroprotective for C.diphtheriae. These kinds of studies are important to know diptheria seroprevalance in different regions.Öğe Investigation of microbial colonization of computer keyboards used inside and outside hospital environments [Hastane ve hastane d?ş? ortamlarda kullan?lan bilgisayarlar?n klavyelerinde mikrobiyal kolonizasyonun araşt?r?lmas?](2008) Do?an M.; Feyzio?lu B.; Özdemir M.; Baysal B.Computers have been commonly used in daily life and at hospitals by medical staff. This study was carried out to search the microbial colonization of computer keyboards and mice used inside and outside hospital environments. Keyboards and mice samples from a total of 398 computers were included to the study, in which 38 were used by doctors and nurses in the hospital clinics (Group 1); 32 by the medical faculty students (Group 2), and 328 by university students (Group 3) in the computer laboratories of Selcuk University, Konya (located at middle Anatolia). Of the computers, 96.7% (n:385) have been found to be colonized by coagulase-negative staphylococci (CoNS), 13.1% (n:52) by gram-positive spore-forming bacilli and 8.8% (n:35) by corynebacteria; followed by Candida spp. (4.2%), gram-negative bacilli (1.7%) [Acinetobacter spp. (n:4), Pseudomonas sp. (n:1), Klebsiella sp. (n:1), E.coli (n:1)], Staphylococcus aureus (1.5%), and molds (Penicillium, Aspergillus; 1.2%). The isolation rates of CoNS were similar between the groups (94,7%, 93.7%, and 97.2%, respectively). However it was noted that all of the gram-negative bacterial isolates (7/38; 18.4%) were from the samples collected from hospital computers (Group 1). Susceptibility rates of CoNS isolates to cefoxitin were detected as 26.2% in Group 1, 79.2% in Group 2, and 91.3% in Group 3. Five out of six S.aureus strains were found susceptible to cefoxitin, except one isolated from a sample of Group 1. Linezolid resistance in both CoNS and S.aureus isolates were not determined in any groups. As a result, according to the data obtained from this study as well as from the other foreign studies, the computer keyboards and mice which are widely used in the hospital settings, are being the source of potential cross contamination in the development of nosocomial infections. Therefore the computers should be cleaned properly frequently and hand washing procedures and disinfection rules should be obeyed after the use of computers before handling this patients.Öğe Investigation of parvovirus B19 seroprevalence in various age groups in central anatolia region, Turkey [Konya bölgesi?nde çşi?tli? yaş gruplarinda parvovi?rus B19 seroprevalansinin araştirilmasi](2010) Türk Da?i H.; Özdemir M.; Baykan M.; Baysal B.Human parvovirus B19 is a small, non-enveloped, icosahedral symmetric, single-stranded DNA virus that can cause a number of diseases, notably erythema infectiosum in children and aplastic crisis in patients with chronic hemolytic disorders. There have been limited data on the epidemiological pattern of parvovirus B19 infection in Turkey. The objective of this study was to investigate the seroprevalence of parvovirus B19 in Konya province (Central Anatolia), Turkey. Parvovirus B19 IgG antibodies were investigated by a commercial ELISA kit (RIDASCREEN, R-Biopharm AG, Germany) in 631 adults (age range: 18-> 60 years) and 542 children (age range: 0-17 years). The overall prevalence of parvovirus B19 IgG antibodies was 28.9%. The rate of parvovirus B19 IgG positivity was 20.7% (112/542) in the 0-17 years age group and was 36% (227/631) in the adult population. No significant difference in seropositivity rates were detected in terms of sex in children and adult group (p>0.05 in both groups). The rates of parvovirus B19 IgG seropositivity were 15.8% in 0-4 years age group, 16% in 5-9 years, 24.2% in 10-14 years, 40.9% in 15-19 years, 34.7% in 20-29 years, 35.5% in 30-39 years, 32.2% in 40-49 years, 37.5% in 50-59 years and 53.8% in > 60 years age group. The seropositivity rates in 0-4 and 5-9 years age groups were lower than the other age groups and the difference was statistically significant (p< 0.05). To determine the prevalence of parvovirus B19 in different age groups In different geographical areas is necessary since this will provide important information about the epidemiology of such infections.Öğe Investigation of viral pathogens during pregnancy in a city region in Turkey [Türki?ye'ni?n bi?r şehri?nde gebeli?k sirasinda vi?ral patojenleri?n araştirilmasi](2011) Özdemir M.; Kalem F.; Feyzio?lu B.; Baysal B.Rubella virus, Cytomegalovirus (CMV) and Herpes simplex virus (HSV)-II are the infectious agents which may cause congenital malformations in the fetus if acquired during pregnancy. Thus these agents must be screened throughout pregnancy. This screening is also important to demonstrate the regional status regarding these agents. In this study; it is aimed to evaluate the seroprevalance of Rubella, CMV and HSV-II in pregnant women in a city region in Turkey. A total of 249 pregnant women who applied to the outpatient clinics of the obstetric and gynaecological department of a university hospital were involved to the study during antenatal screening in the first trimester of pregnancy. After separation of blood sample sera samples were tested for antibodies to Rubella, CMV and HSV-II. While Rubella and CMV antibodies were tested using Enzyme Linked Floeresan Antibody method, HSV-II was tested using ELISA. The mean age of the participants in this study was 28.4± 3.4 years. The seropositivity for anti-Rubella IgM, anti-Rubella IgG, anti-CMV IgM, anti-CMV IgG, anti-HSV-II IgM, and anti-HSV-II IgG were 0.4%, 95.9%, 0%, 98.7%, 0%, 4.4%, respectively. Although the seropositivity of HSV-II was very low, seropositivities of Rubella and CMV were very high in our area of investigation. Viruses causing congenital infection and malformation must be screened in pregnant women individually and avidity tests must be applied in suspicious cases. In addition the seroprevelance of these viral infections should be known in every population in order to take preventive measures and prepare local and general health policies.Öğe Isolation of Citrobacters in various infections and their antimicrobial sensitivity rates(Veteriner Fakultesi Dergisi, 2011) Kurtoğlu M.G.; Opus A.; Özdemir M.; Baysal B.The samples for culture from the patients admitted to Konya Research and Education Hospital between January 2009 and July 2010 were investigated. The obtained Citrobacter strains were studied for antimicrobial sensitivity rates and their distribution ratesto the clinics. The samples obtained were inoculated onto related culture media. Aseptic body samples were incubated at 37°C into bottles of BACTEC 9120 blood culture system. For the identification and antimicrobial sensitivities of yielding bacteria, panels of Phoenix-100 automized identification device were used. Mean age rate of patients in whom Citrobacter strains were determined was 41.29±4.345. Of all samples with Citrobacter strains, 48% were isolated from urine, 29% from surgical wounds, 11% from sputum, 2% from peritoneal fluid, and 2% from vaginal samples. Of total 52 Citrobacter strains, the species level distribution was 46% C. freundii, 21% C. youngae, 15% C. koseri, 10% C. braakii, 6% C. farmeri and 2% C. wermanii. The distribution of samples with Citrobacter strains to the clinics were 29% adult intensive care unit (ICU), 21% pediatric, 11% general surgery, 10% in neonatal ICU, 10% plastic surgery, 7% urology, 6% burn unit, and 6% nephrology department. The most sensitive antimicrobial agents to Citobacter strains were amikacin (100%), meropenem (100%), imipenem (96%) and piperacillin/tazobactam (96%).Öğe The sensitivity of Staphylococcus aureus strains isolated from different clinical specimens to cefuroxime and some other antimicrobial agents(1994) Tuncer E.I.; Baykan M.; Kart H.; Baysal B.Three hundred and eight S. aureus strains isolated from specimens of urine, exudate and throat swabs were sent to the Microbiology Laboratory of Selcuk University School of Medicine and were examined for susceptibility to antimicrobial agents such as cefuroxime (CXM), ofloxacin (OFX), erythromycin (E), trimethoprim + sulfamethoxazole (SXT), ampicillin + sulbactam (SAM), lincomycin (L), amoxicillin + clavulanic acid (AMC), penicillin-G (P) and ampicillin (AM). The isolates of S. aureus were found to be susceptible to CXM, OFX, E, SXT, SAM, L, AMC, P and AM in the rates of 96.7%, 96.4%, 82.5%, 66.5%, 63.3%, 61.4%, 54.8%, 8.3%, 5.8% respectively.
