Yazar "Bender, Onur" seçeneğine göre listele
Listeleniyor 1 - 5 / 5
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A comparative study of Bulgarian and Turkish Asphodeline lutea root extracts: HPLC-UV profiles, enzyme inhibitory potentials and anti-proliferative activities against MCF-7 and MCF-10A cell lines(ELSEVIER SCIENCE BV, 2015) Lazarova, Irina; Zengin, Gökhan; Bender, Onur; Zheleva-Dimitrova, Dimitrina; Uysal, Şengül; Ceylan, Ramazan; Gevrenova, RenetaAsphodeline lutea (L.) Rchb. is a wild plant traditionally used as a food in the Mediterranean region. The alcoholic extracts of A. lutea roots from different origins (Bulgarian and 'Rakish) were investigated for anti-cholinesterase, anti-tyrosinase, anti-amylase, anti-glycosidase and anti-proliferative activity (against MCF-7 and MCF-10A cell lines). A validated HPLC method for the determination of two anthraquinones, two bianthraquinones, two naphthalenes, two flavonoids and one hydroxycinnamic acid was developed due to the presence of these components in the genus Asphodeline. In addition, total anthraquinones content was determined spectrophotometrically. A. lutea from Bulgaria demonstrated higher level of total anthraquinones (0.81 +/-0.05 mg g(-1)). A naphthalene derivative (2-acetyl-1,8-dimethoxy-3-methylnaphthalene) was the major compound in Bulgarian accession (0.96 +/- 0.03 mg g(-1)), while caffeic acid was the main analyte in Turkish sample (0.42 +/- 0.06 mg g(-1)). Turkish extracts were more potent as cholinesterase and tyrosinase inhibitors but less active as antidiabetic agent. The higher anti-proliferative effect was presented by Bulgarian extract with IC50 values 120 +/- 16.8 mg mL(-1) (for MCF-7) and 256 +/- 16.1 mg mL(-1) (for MCF-10A). (C) 2015 Elsevier Ltd. All rights reserved.Öğe Integration of in vitro and in silico perspectives to explain chemical characterization, biological potential and anticancer effects of Hypericum salsugineum: A pharmacologically active source for functional drug formulations(PUBLIC LIBRARY SCIENCE, 2018) Bender, Onur; Llorent-Martinez, Eulogio J.; Zengin, Gökhan; Mollica, Adriano; Ceylan, Ramazan; Molina-Garcia, Lucia; Fernandez-de Cordova, Maria Luisa; Atalay, ArzuThe genus Hypericum is one of the most popular genera in both traditional medicine and scientific platform. This study is designed to provide conceptual insights on the biological potential and chemical characterization of H. salsugineum, which is endemic to Turkey. The qualitative and quantitative phenolic content of the extracts was characterized by HPLC-ESI-MSn. Biological efficiency was investigated by enzyme inhibitory assays (cholinesterases, tyrosinase, amylase, and glucosidase) and anti-cancer efficacy tests (anti-proliferative activities with the iCELLigence technology, colony formation and wound healing scratch assays). Phenolic acids (3-O-caffeoylquinic, 5-O-caffeoylquinic, and 4-O-caffeoylquinic acids) were the predominant group in the studied extracts, although several flavonoids were also detected and quantified. The extracts exhibited good inhibitory effects on tyrosinase and glucosidase, while they had weak ability against cholinesterases and amylase. Computational studies were also performed to explain the interactions between the major phenolics and these enzymes. The extracts displayed significant anti-cancer effects on breast carcinoma cell lines. Our findings suggest that Hypericum salsugineum could be valued as a potential source of biologically-active compounds for designing novel products.Öğe Investigating and comparing antioxidant capacity of different region's propolis and their effect on chromosome instability(LIPPINCOTT WILLIAMS & WILKINS, 2015) Yılmaz, Sultan Çiftçi; Akpınar, Zeynep N.; Ceylan, Ramazan; Gündüz, Esra; Bender, Onur; Zengin, Gökhan; Aktümsek, Abdurrahman[Abstract not Available]Öğe Multiple pharmacological targets, cytotoxicity, and phytochemical profile of Aphloia theiformis (Vahl.) Benn(ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2017) Picot, Marie Carene Nancy; Bender, Onur; Atalay, Arzu; Zengin, Gökhan; Loffredo, Loic; Hadji-Minaglou, Francis; Mahomoodally, Mohamad FawziAphloia theiformis (Vahl.) Benn. (AT) is traditionally used in Sub-Saharan African countries including Mauritius as a biomedicine for the management of several diseases. However, there is a dearth of experimental studies to validate these claims. We endeavoured to evaluate the inhibitory effects of crude aqueous extract as traditionally used together with the crude methanol extracts of AT leaves on urease, angiotensin (I) converting enzyme (ACE), acetylcholinesterase (AChE), cholesterol esterase (CEase), glycogen phosphorylase a (GPa), and glycation in vitro. The crude extract showing potent activity against the studied enzymes was further partitioned using different solvents of increasing polarity. The enzyme inhibitory and antiglycation activities of each fraction was assessed. Kinetic of inhibition of the active crude extract/fractions on the aforementioned enzymes was consequently determined using Line-weaver- Burk plots. An ultra-high performance liquid chromatography (UHPLC-UV/MS) system was used to establish the phytochemical profile of AT. The real time cell analysis system (iCELLigence (TM)) was used to monitor any cellular cytotoxicity of AT. Crude methanolextract (CME) was a potent inhibitor of the studied enzymes, with IC50 ranging from 696.22 to 19.73 mu g/mL. CME (82.5%) significantly (p < 0.05) inhibited glycation and was comparable to aminoguanidine (81.5%). Ethyl acetate and n-butanol fractions of CME showed non-competitive, competitive, and uncompetitive mode of inhibition against ACE, CEase, and AChE respectively. Mangiferin, a xanthone glucoside was present in CME, ethyl acetate, and n-butanol fractions. Active extract/fractions were found to be non-cytotoxic (IC50 > 20 mu g/mL) according to the U.S National Cancer Institute plant screening program. This study has established baseline data that tend to justify the traditional use of AT and open new avenues for future biomedicine development. (C) 2017 Elsevier Masson SAS. All rights reserved.Öğe Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L-czeczottianus and L-nissolia(FRONTIERS MEDIA SA, 2017) Llorent-Martinez, Eulogio J.; Zengin, Gökhan; Cordova, Maria L. Fernandez-De; Bender, Onur; Atalay, Arzu; Ceylan, Ramazan; Mollica, AdrianoMembers of the genus Lathyrus are used as food and as traditional medicines. In order to find new sources of biologically-active compounds, chemical and biological profiles of two Lathyrus species (L. czeczottianus and L. nissolia) were investigated. Chemical profiles were evaluated by HPLC-ESI-MSn, as well as by their total phenolic and flavonoid contents. In addition, antioxidant, enzyme inhibitory, and cytotoxic effects were also investigated. Antioxidant properties were tested by using different assays (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation). Cholinesterases (AChE and BChE), tyrosinase, alpha-amylase, and alpha-glucosidase were used to evaluate enzyme inhibitory effects. Moreover, vitexin (apigenin-8-C-glucoside) and 5-O-caffeoylquinic acid were further subjected to molecular docking experiments to provide insights about their interactions at molecular level with the tested enzymes. In vitro cytotoxic effects were examined against human embryonic kidney cells (HEK293) by using iCELLigence real time cell analysis system. Generally, L. czeczottianus exhibited stronger antioxidant properties than L. nissolia. However, L. nissolia had remarkable enzyme inhibitory effects against cholinesterase, amylase and glucosidase. HPLC-ESI-MSn analysis revealed that flavonoids were major components in these extracts. On the basis of these results, Lathyrus extracts were rich in biologically active components; thus, these species could be utilized to design new phytopharmaceutical and nutraceutical formulations.
