Integration of in vitro and in silico perspectives to explain chemical characterization, biological potential and anticancer effects of Hypericum salsugineum: A pharmacologically active source for functional drug formulations
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
PUBLIC LIBRARY SCIENCE
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The genus Hypericum is one of the most popular genera in both traditional medicine and scientific platform. This study is designed to provide conceptual insights on the biological potential and chemical characterization of H. salsugineum, which is endemic to Turkey. The qualitative and quantitative phenolic content of the extracts was characterized by HPLC-ESI-MSn. Biological efficiency was investigated by enzyme inhibitory assays (cholinesterases, tyrosinase, amylase, and glucosidase) and anti-cancer efficacy tests (anti-proliferative activities with the iCELLigence technology, colony formation and wound healing scratch assays). Phenolic acids (3-O-caffeoylquinic, 5-O-caffeoylquinic, and 4-O-caffeoylquinic acids) were the predominant group in the studied extracts, although several flavonoids were also detected and quantified. The extracts exhibited good inhibitory effects on tyrosinase and glucosidase, while they had weak ability against cholinesterases and amylase. Computational studies were also performed to explain the interactions between the major phenolics and these enzymes. The extracts displayed significant anti-cancer effects on breast carcinoma cell lines. Our findings suggest that Hypericum salsugineum could be valued as a potential source of biologically-active compounds for designing novel products.
Açıklama
Anahtar Kelimeler
Chemical characterization, Hypericum salsugineum, Anticancer effect, Functional drug formulation
Kaynak
PLOS ONE
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
13
Sayı
6
Künye
Bender, O., Martinez, E. J. L., Zengin, G., Mollica, A., Ceylan, R., Garcia, L. M., Cordova, M. L. F., Atalay, A. (2018). Integration of in Vitro and in Silico Perspectives to Explain Chemical Characterization, Biological Potential and Anticancer Effects of Hypericum Salsugineum: A Pharmacologically Active Source for Functional Drug Formulations. Plos One, 13(6).
