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  • Küçük Resim
    Öğe
    Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria Siceraria (Molina) Stand.)
    (Turkiye Klinikleri Journal of Medical Sciences, 2009) Yalçın Mendi, Yeşim; İpek, Muzaffer; Buzkan, Nihal; Aka Kaçar, Yıldız; Çürük, Sebahattin
    Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l-1) and IAA (0, 0.25, and 0.5 mg l-1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l-1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.
  • Küçük Resim Yok
    Öğe
    Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)
    (TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2009) Mendi, Yesim Yalcin; Ipek, Muzaffer; Buzkan, Nihal; Kancar, Yildiz Aka; Curuk, Sebahattin
    Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.). germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0. and 2.0 mg l(-1)) and IAA (0, 0.25, and 0.5 mg l(-1)). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l(-1) of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.
  • Küçük Resim Yok
    Öğe
    Regeneration and Histological analysis of regeneration in bottle gourd (Lagenaria siceraria (Molina)Stand.)
    (2009) Mendi, Yeşim Yalçın; İpek, Muzaffer; Buzkan, Nihal; Kaçar, Yıldız Aka; Çürük, Sabahattin
    Bu çalışmada, hibrit su kabağı (Lagenaria siceraria (Molina) Stand.) çeşidi olan Emphasis’in hem ışık hem de karanlık koşullarda çimlendirilmiş olan bitkilerinden alınan 2 eksplant tipi (proksimal ve flamingo-bill), BA (0.0, 1.0 ve 2.0 mg l–1) ve IAA (0.0, 0.25 ve 0.5 mg l–1)’in 9 farklı kombinasyonunu içeren MS rejenerasyon ortamlarında kültüre alınmıştır. Eksplant tiplerinin karşılaştırıldığı çalışma sonuçları, sürgün oluşumu üzerine flamingo-bill tipi eksplantının proksimal eksplanta nazaran daha iyi sonuç verdiğini ortaya koymuştur. Karanlık ve aydınlık koşullarda çimlendirilmiş olan flamingo-bill eksplantlarında optimum sürgün oluşum kapasitesi, 1 mg l–1 BA içeren MS ortamından % 44 ve % 36 olarak saptanmıştır. Histolojik analizler, eksplantlardaki hücre bölünmesinin rejenerasyon ortamına alındıktan 3 gün sonra başladığını, primordiyum oluşumunun ise 5 ila 7. günler arasında gözlendiği belirlemiştir. Meristematik yapının farklılaşması ilk olarak 9. günde gözlenmiş, 9 ve 12. günler arasında ise oluşum tamamlanmıştır.