Regeneration and Histological analysis of regeneration in bottle gourd (Lagenaria siceraria (Molina)Stand.)
Küçük Resim Yok
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, hibrit su kabağı (Lagenaria siceraria (Molina) Stand.) çeşidi olan Emphasis’in hem ışık hem de karanlık koşullarda çimlendirilmiş olan bitkilerinden alınan 2 eksplant tipi (proksimal ve flamingo-bill), BA (0.0, 1.0 ve 2.0 mg l–1) ve IAA (0.0, 0.25 ve 0.5 mg l–1)’in 9 farklı kombinasyonunu içeren MS rejenerasyon ortamlarında kültüre alınmıştır. Eksplant tiplerinin karşılaştırıldığı çalışma sonuçları, sürgün oluşumu üzerine flamingo-bill tipi eksplantının proksimal eksplanta nazaran daha iyi sonuç verdiğini ortaya koymuştur. Karanlık ve aydınlık koşullarda çimlendirilmiş olan flamingo-bill eksplantlarında optimum sürgün oluşum kapasitesi, 1 mg l–1 BA içeren MS ortamından % 44 ve % 36 olarak saptanmıştır. Histolojik analizler, eksplantlardaki hücre bölünmesinin rejenerasyon ortamına alındıktan 3 gün sonra başladığını, primordiyum oluşumunun ise 5 ila 7. günler arasında gözlendiği belirlemiştir. Meristematik yapının farklılaşması ilk olarak 9. günde gözlenmiş, 9 ve 12. günler arasında ise oluşum tamamlanmıştır.
Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l–1) and IAA (0, 0.25, and 0.5 mg l–1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l–1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.
Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l–1) and IAA (0, 0.25, and 0.5 mg l–1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l–1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.
Açıklama
Anahtar Kelimeler
Orman Mühendisliği
Kaynak
Turkish Journal of Agriculture and Forestry
WoS Q Değeri
Scopus Q Değeri
Cilt
33
Sayı
2
