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    Determinations of mRNA's for TGF?2, 3 in mouse testes and ovaries after a five days injection with Glycine or a Serine and Threonine mixture
    (2008) Ciftci H.B.
    This study is presented to test the effect of injecting a mixture of Serine/Threonine or Glycine amino acids to determine and measure the changes in mRNAs expression for TGF?2, 3 within the mouse testes and the ovaries. Male (n = 30) and female (n = 30) mice (CD-1 strain) were injected, once a day for 5 days, with 0.2 mL Saline (control), 0.2 mL Saline with a mixture of Senne/Threonine (test) containing 0.26 ?g L Threonine, 0.13 ?g L Serine or 38 ng Glycine (Test). Total RNA was extracted and transferred on to a nylon membrane then probed with 32P-lebelled cDNA probes. The expression of mRNA for TGF?2 increased in male mice while there were no differences in female mice. This study shows that TGF? 2, 3 are expressed both in the ovary and testis and their expressions were increased by the injection of Serine/Threonine or glycine. © Medwell Journals, 2008.
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    Effect of supplementation of ovine oviductal cells with LH and gestradiol-17? on embryo development
    (2008) Ciftci H.B.; Zulkadir U.; Boztepe S.; Ozturk A.; Dag B.; Yetisir R.
    The aim of this research was to study the effect of supplementing the oviductal cells with Luteinizing hormone (LH) and Oestradiol - 17? (E 2) on sheep embryo development in vitro. Addition of 5?g/ml LH, 5?g/ml E2 to embryo culture significantly increased the percentage of embryos over the control group. Addition of LH and E2 to the culture of zygotes containing oviductal cells, caused further significant increases in the percentages embryos at morulae, blastocyst and hatched stages over the control and LH-E2 added group by 28.75, 19.88 8.89% and 15.58, 11.91, 6.39% respectively.