Determinations of mRNA's for TGF?2, 3 in mouse testes and ovaries after a five days injection with Glycine or a Serine and Threonine mixture
Küçük Resim Yok
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study is presented to test the effect of injecting a mixture of Serine/Threonine or Glycine amino acids to determine and measure the changes in mRNAs expression for TGF?2, 3 within the mouse testes and the ovaries. Male (n = 30) and female (n = 30) mice (CD-1 strain) were injected, once a day for 5 days, with 0.2 mL Saline (control), 0.2 mL Saline with a mixture of Senne/Threonine (test) containing 0.26 ?g L Threonine, 0.13 ?g L Serine or 38 ng Glycine (Test). Total RNA was extracted and transferred on to a nylon membrane then probed with 32P-lebelled cDNA probes. The expression of mRNA for TGF?2 increased in male mice while there were no differences in female mice. This study shows that TGF? 2, 3 are expressed both in the ovary and testis and their expressions were increased by the injection of Serine/Threonine or glycine. © Medwell Journals, 2008.
Açıklama
Anahtar Kelimeler
Glycine, Mice, RNA, Serine, TGF?, Threonine
Kaynak
Journal of Animal and Veterinary Advances
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
7
Sayı
1
