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    Agresif periodontitisli hastalarda tedavi öncesi ve sonrası periodontal patojen profili
    (Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2008-02-26) Dündar, Niyazi; Durak, Yusuf
    Bu çalışmada; Agresif periodontitisli (AP) hastalarda, periodontal tedavi öncesi patojen bakteri profili çıkarılmış, tedavi sonrası 1. ve 3. aylarda profildeki değişiklikler izlenmiş ve bakteriyell rekolonizasyon değerlendirilmiştir. Bu çalışma Agresif periodontitisli (AP=10), Gingivitisli (G=10) ve Sağlıklı (S=10) toplam 30 kişi üzerinde yapılmıştır. Periodontopatojenler Polymerase Chain Reaction (PCR) yöntemi ile araştırılmış ve elde edilen sonuçlar Real-time PCR ile doğrulanmaya çalışılmıştır. Başlangıçta alınan subgingival plak örneklerinde; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia ve Treponema denticola bakteri suşları sıra ile %70, %70, %100 ve %40 oranında tespit edilmiştir. P. gingivalis pozitif yedi hastanın, üçünde tip I fimA ve dördünde tip II fimA belirlenmiştir. Faz I periodontal tedavi sonrası 1. ayda periodontopatojenlerde yüksek oranda azalma gözlenirken, 3. ayda periodontopatojenlerin rekolonizasyon gösterdiği izlenmiştir. AP hastaların idame fazında düzenli takip edilmeleri ve ilave kemoterapinin de düşünülmesi sonucuna varılmıştır.
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    Characterization of Klebsiella pneumoniae Strains Isolated from Urinary Tract infections: Detection of ESBL Characteristics, Antibiotic Susceptibility and RAPD Genotyping
    (POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2013) Aladağ, Mustafa Onur; Uysal, Ahmet; Dündar, Niyazi; Durak, Yusuf; Güneş, Erdoğan
    In this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K. pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%),the resistance rates of non ESBL producing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.
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    Comparison of Er,Cr:YSGG Laser and Hand Instrumentation on the Attachment of Periodontal Ligament Fibroblasts to Periodontally Diseased Root Surfaces: An In Vitro Study
    (Amer Acad Periodontology, 2010) Hakkı, Sema S.; Korkusuz, Petek; Berk, Gizem; Dündar, Niyazi; Sağlam, Mehmet; Bozkurt, Buket; Purali, Nuhan
    Background: This study investigates the effects of erbium, chromium: yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation and hand instrumentation on the attachment of periodontal ligament (PDL) fibroblasts to periodontally involved root surfaces. Methods: Twenty-four single-rooted periodontally involved human teeth (test groups), and six healthy premolar teeth extracted for orthodontic reasons (control group) were included in this study. A total of 45 root slices were obtained from all selected teeth and assigned to the following five groups: 1) untreated healthy group (+control); 2) untreated periodontally diseased group ( control); 3) hand instrumentation group (scaled Gracey); 4) laser I, Er,Cr:YSGG laser irradiation setting-I (short pulse); and 5) laser II, Er,Cr:YSGG laser irradiation setting-II (long pulse). All of the root slices were autoclaved in phosphate buffered saline and slices were placed onto cell culture inserts. PDL fibroblasts were placed at the density of 80,000 cells on the root plate (5 x 6 mm) and incubated for 48 hours and transferred to 24-well plates. The attachment PDL fibroblasts on the root plates were observed using confocal microscopy (at 12 hours and on days 3 and 7) and scanning electron microscopy (at 12 hours and day 3). 3- (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was performed on day 5 for PDL fibroblast survival. Results: 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay shows that whereas laser-treated specimens showed a significantly higher cell density, the Gracey-treated group showed a lower cell density compared to the positive control group (P < 0.05). Based on confocal microscopy, apparent reduction was observed in the attachment of PDL cells to the periodontally diseased root surfaces. In the laser and Gracey groups, cells looked well-oriented to the root surfaces. Laser-treated groups provided suitable environment for cell adhesion and growth. Laser I treatment was more favorable for the attachment of PDL compared to scaled Gracey, laser II, and even healthy root surfaces. Conclusion: The results of the study indicate that short-pulse laser setup (laser I) looks more promising regarding the attachment, spreading, and orientation of PDL cells.
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    Effect of anatomical location (mandible vs maxilla) of dental implants on the BMP-2, BMP-7, sRANKL and OPG levels in periimplant crevicular fluid during osseointegration. A pilot study
    (Selçuk Üniversitesi, 2019) Sağlam, Mehmet; Dolanmaz, Doğan; Koçak, Emrah; Gürsoytrak, Burcu; İnan, Özgür; Dündar, Niyazi; Hakkı, Sema S.
    Background: The aim of this study was to investigate levels of bone morphogenetic protein-2 (BMP-2), BMP-7, soluble receptor activator of nuclear factor-kB ligand (sRANKL) and osteoprotegerin (OPG) in the peri-implant crevicular fluid (PICF) of implants placed in both maxilla and mandible during the osseointegration period. Materials and Methods: Thirty-three patients (17 females and 16 males; mean age 47.03±11.23 years) were included in this study. A total of 33 implants were placed in both of maxilla (Group 1/n=18) and mandible (group 2/n=15). Peri-implant crevicular fluid (PICF) samples, modified plaque index (MPI), gingival index (GI) and probing depth (PD) measurements were obtained at 1 and 3 months after surgery. PICF levels of BMP-2/-7, sRANKL and OPG were analyzed by ELISA. Results: No complications were observed during the healing period. No significant differences were observed in the PICF levels of sRANKL, OPG, BMP-2 and BMP-7 and evaluated clinical parameters between groups at any time point (p>0.05). While PICF volume of group 2 was greater than group 1 at first month, PICF volume of group 1 was greater than group 2 at 3 months (p<0.05). There was a positive correlation between sRANKL levels and PICF volume (p<0.05) and a strong correlation between BMP-2 and BMP-7 (p<0.01). Conclusion: The results of this pilot study didn’t show any significant difference in PICF levels of BMP-2, BMP-7, sRANKL, and OPG in terms of anatomic location of dental implants. Further welldesigned studies should be carried out to evaluate the relationship between bone related biomarkers and anatomic location of dental implants.
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    Effects of Root Planing Procedures with Hand Instrument or Erbium, Chromium: Yttrium-Scandium-Gallium-Garnet Laser Irradiation on the Root Surfaces: A Comparative Scanning Electron Microscopy Study
    (Springer London Ltd, 2010) Hakkı, Sema S.; Berk, Gizem; Dündar, Niyazi; Sağlam, Mehmet; Berk, Nukhet
    The purpose of this study was to investigate the efficiency of hand instrumentation and laser irradiation on calculus removal from the root surfaces, in vitro. Thirty-two human teeth, extracted for periodontal reasons, were used in this study. Root surfaces of single-rooted teeth were treated by different methods including (1) conventional hand instruments; (2) hand instruments and tetracycline-hydrochloride (Tet-HCl); (3) erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation, setting I (short pulse); (4) Er,Cr:YSGG laser irradiation, setting II (long pulse). Three premolar teeth, extracted for orthodontic reasons, served as control. The morphology of the root surfaces was evaluated by light and scanning electron microscopy. Energy dispersive X-ray (EDX) analysis was performed to compare the mineral content of root surfaces treated with hand instrumentation and lasing procedures. The results of this study demonstrated that all treatments were efficient in calculus removal from the root surfaces. Thermal changes, including melting and carbonization, were not observed in either lasing procedure. The surface was rougher in the laser groups than in the groups treated with hand instruments. Moreover, roughness was greater in the long-pulse laser setting than in the short-pulse setting. While increased calcium (Ca) and decreased phosphate (P) (weight concentration percent) were observed in all treatments when compared with the control, laser procedures resulted in a more similar mineral content than in the groups treated with hand instruments. Based on these findings, laser procedures, when used in appropriate settings, are capable of performing scaling and root planing in the treatment of periodontitis. It may be concluded that short pulse laser may be more suitable for the micro-morphology of the root surface. However, additional in vitro and clinical studies are necessary to clarify the success of laser in periodontal therapy.
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    Gingival crevicular fluid levels of monocyte chemoattractant protein-1 in patients with aggressive periodontitis
    (WILEY, 2017) Günpınar, Şadiye Coşkuner; Alptekin, Nilgün Özlem; Dündar, Niyazi
    OBJECTIVES: The purpose of this study was to investigate the gingival crevicular fluid (GCF) levels of monocyte chemoattractant protein (MCP)-1 in aggressive periodontitis (AgP) and whether GCF MCP-1 levels differ among localized (L) AgP and generalized (G) AgP. MATERIAL AND METHODS: A total of 160 subjects including 80 AgP and 80 age- and gender-matched periodontally healthy (H) controls were recruited in this cross-sectional study (NCT02927704). GCF samples were collected from 160 patients including 50 LAgP, 30 GAgP, and 80 H. Volume of GCF was measured by Periotron 8000 (R), and enzyme-linked immunosorbent assay was used to assess MCP-1 levels. RESULTS: Compared to H controls, all clinical parameters and total amounts (pg 30 s(-1)) of MCP-1 were significantly higher in subjects with LAgP and GAgP (P < 0.05). Although concentrations of GCF MCP-1 did not differ between LAgP and GAgP (P > 0.05), total amounts of MCP-1 were higher in GAgP than LAgP (P < 0.05). CONCLUSION: It can be concluded that the total amount of MCP-1 level in GCF may be a potential determinant in AgP subjects. Increased MCP-1 levels in line with the degree of periodontal destruction in GAgP patients reveal that MCP-1 can be used to understand the disease pathogenesis of LAgP and GAgP.
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    Tip 2 diyabetik ratlarda Rhaponticoides iconiensis (Tülüşah) ekstresinin antidiyabetik etkisinin araştırılması
    (Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, 2025) Dündar, Niyazi; Öztürk, Bahadır
    Diabetes Mellitus (DM), insülin salınımı, insülin etkisi veya bu faktörlerin her ikisinde de bozukluk nedeniyle ortaya çıkan hiperglisemi ile karakterize kronik metabolik bir hastalıktır. Yaşlanan nüfus, kentleşme ve değişen yaşam tarzları diğer kronik hastalıklarla birlikte diyabet epidemisini de hızla artırmaktadır. Hızla artan diyabet insidansı ile birlikte, hayati organların diyabetik hasardan korunması, tedavi edilmesi ve özellikle Tip 2 diyabetin toplam diyabet vakalarının yaklaşık %90'ını oluşturduğu göz önüne alındığında, bu konu ciddi önem taşımaktadır. Oksidatif bir stres hastalığı olan diyabet tedavisinde antioksidan aktiviteye sahip bileşiklerin olumlu etkileri olabilmektedir. Bu çalışmada, yan etkisi olabileceği düşünülen ilaçlara destek olarak Rhaponticoides iconiensis'in antioksidan etki ve içerdiği fenolik bileşenlerinden dolayı antidiyabetik etki potansiyeline sahip olması açısından yüksek yağlı diyet ve STZ ile Tip 2 Diyabet oluşturulan ratların tedavisine ek olarak kullanılabilirliği araştırıldı. Rhaponticoides iconiensis (Rİ) kök, yaprak ve çiçeklerinden elde edilen çeşitli ekstrelerin içerdiği fitokimyasal bileşiklerin antidiyabetik içerikleri yapılan çalışmalarla ortaya konmuş ancak antidiyabetik etkisi değerlendirilmemiştir. Bu nedenle ön görülen tez çalışmasında Rİ yaprak ekstresinin, önceden ortaya konmuş antioksidan, α-amilaz ve α-glukozidaz aktivitelerine etkisinin ratlar üzerinde in vivo şartlarda değerlendirilmesi planlandı. Rİ ekstresinin ratlarda oral uygulanmasından elde edilen sonuçlarla hiperglisemi, dislipidemi, kronik inflamasyon ve oksidatif stresi azaltarak Tip 2 Diyabetes Mellitus (T2DM) tedavisi ve diyabetin yol açtığı komplikasyonların önlenmesinde takviye olarak etkinliğini TAS, TOS, TNFα, IL-1β, IL-6, WNT5A, SFRP5 biyokimyasal belirteçler üzerinden ve Anti- İnsülin, Anti-Kaspaz 3, Anti-TGFβ1 antikor işaretleme, Hematoksilen & Eozin (H&E) boyama ile histopatolojik olarak araştırıldı. Rİ ekstresinin antidiyabetik etkinliği tedavide kullanılan Akarboz (Glukobay®) ile kıyaslamalı olarak araştırıldı. 46 rat; Kontrol, DM, DM + 30 mg/kg Akarboz, DM + 100 mg/kg Rİ ekstresi (Rİ1), DM + 200 mg/kg Rİ ekstresi (Rİ2), DM + 300 mg/kg Rİ ekstresi (Rİ3) olmak üzere 6 gruba ayrıldı. Deneysel diyabet, yüksek yağlı diyet (YYD) ve bir hafta ara ile iki doz streptozotosinin intraperitoneal (i.p.) olarak enjeksiyonu ile T2DM oluşturuldu. Rİ ektresinin tüm dozları ve Akarboz 28 gün boyunca gastrik gavaj yolu ile verildi. Sonuç olarak; Rİ'nin metanol ile elde edilen biyoaktif bileşenlerce zengin ekstresinin T2DM oluşturulmuş ratlar üzerinde akarboz benzeri antidiyabetik etki yaparak mevcut tedavilere takviye olabileceği sunulan bu çalışma ile gösterilmiştir.