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Öğe Antibiotic susceptibility of Lactococcus garvieae in rainbow trout (Oncorhyncus mykiss) farms(NATL VETERINARY RESEARCH INST, 2008) Kav, Kursat; Erganis, OsmanThe aim of the study was to describe bilateral exophthalmos "pop eye syndrome" in rainbow trout (Onchorynchus mykiss) fisheries in the Konya region and to determine effective antibiotic treatments. Between June 2002 and August 2004, 180 ill fish were obtained from 6 rainbow trout fisheries where the disease had been observed. Lactococcus garvieae strains were isolated from fish tissues during different periods. All the isolates were found susceptible to penicillin-G, ampicillin, amoxycillin, ampicillin+sulbactam, amoxicillin/clavulanic acid, vancomycin, ciprofloxacin, marbofloxacin, chloramphenicol, florfenicol, erythromycin, oxytetracycline, cefoperazone, sulbactam/cefoperazone, and novobiocin. It seems that beta- lactam antibiotics are preferred in the treatment of L. garvieae infection in the rainbow trout farms.Öğe Assessment of Antibacterial Activity of Endorez(Mosby-Elsevier, 2006) Eldeniz, Ayçe Ünverdi; Erdemir, Ali; Hadimli, Hasan Hüseyin; Belli, Sema; Erganis, OsmanObjective. The aim of this in vitro study was to evaluate antibacterial activity of a new resin based sealer, EndoREZ in comparison with 5 other sealers: AH 26, Diaket, Sultan, Apexit, and RoekoSeal. Study design. The effect of 6 different sealers oil the growth of 3 bacteria (Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa) was measured using the agar diffusion test (ADT) and direct contact test (DCT). For ADT, 200 mu L bacterial suspensions were spread oil agar plates and freshly mixed sealers were applied to uniform wells punched in the agar. The zones of inhibition of bacterial growth were measured at 24 hours, 48 hours, 7 days, and 10 days. For DCT, 2 sets of sealers were prepared: fresh and 24-hour samples. Fresh samples were used within 20 minutes of mixing time while 24-hour samples were allowed to set it) a humid atmosphere at 37 degrees C for 24 hours before testing. Sealers were mixed and placed oil the walls of microliter plate wells and 10-mu L bacterial Suspensions were allowed to directly contact the sealers for I hour. Fresh media were added and 15 mu L were transferred from this plate to another plate containing fresh medium (215 mu L). Bacterial growth of this last plate was then measured using spectrophotometer every hour over 16 hours. Results. ADT results indicated that EndoREZ, Apexit, and RoekoSeal did not show any, antibacterial activity. In DCT results, AH 26 and Sultan were potent bacterial growth inhibitors. Conclusion. EndoREZ is not as potent a bacterial growth inhibitor as Sultan and AH 26.Öğe Bacillus anthracis Isolated from a Dog in Turkey(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2015) Sayin, Zafer; OzgurOzdemir; Erganis, Osman; Hadimli, Hasan Huseyin; Sakmanoglu, Asli; Atil, Eray; Sanioglu, GokcenurAn Anatolian Akbash shepherd dog died suddenly, without any clinical signs in Konya, Turkey. Performing necropsy, pathological examination, a culture test, laboratory tests and a multiplex-PCR of bacteria isolated from the dog revealed an anthrax and identified the bacteria as Bacillus anthracis (B. anthracis). The chromosomal gene sequence of bacteria was 99% identical in the GenBank under accession numbers CP002091 (B. anthracis str. H9401), CP001598 (B. anthracis str. A0248) and CP001215 (B. anthracis str. CDC684). Antimicrobial susceptibility test was performed and cefuroxime, cefquinome, sulfamethoxazole-trimethoprim, chloramphenicol, tetracycline and rifampicin resistance were seen in isolate. In this case, how the dog was infected with B. anthracis could not be determined. (C) 2014 PVJ. All rights reservedÖğe Clinical Efficacy of Florfenicol in the Treatment of Calf Respiratory Tract Infections(Royal Netherlands Veterinary Assoc, 2002) Aslan, V.; Maden, Mehmet; Erganis, Osman; Birdane, Fatih Mehmet; Çorlu, M.This paper reports on a study of the aetiology of calf pneumonia and the clinical efficacy of florfenicol, a new antibiotic in Turkey. Twenty-seven weaned and unweaned calves (13 males and 14 females) between I and 16 months of age brought to the clinics of Selcuk University, Faculty of Veterinary Science. Broncho-alveolar lavage (BAL) fluid samples were taken from the animals diagnosed to have upper respiratory tract infection associated with bronchitis (N=2), bronchitis (N=5), bronchopneumonia (N=4), pneumonia (N=3), pleuropneumonia (N=11), bronchopneumonia plus pulmonary oedema (N=2) based on the results of the clinical and laboratory examinations. Then microbiological isolation and antibiotic culturing were performed. The animals were treated with I ml/15 kg (20 mg/kg) florfenicol (Nuflor(R), DIF) twice within 48 hours via intramuscular injection. At the end of the treatment, 23 of the weaned and unweaned calves were completely healed, I calf had died and 3 calves showed no healing. The results of BAL samples and microbiological examinations of the 3 calves that did not respond to the treatment indicated that these cases were affected by mixed infections of yeasts, fungi, and bacteria. Widespread pleuropneumonia was observed. According to the results of the microbiological examination of the BAL samples, Mannheimia (Pasteurella) haemolytica had the highest isolation rate (25%) compared with the other isolated bacteria, namely, Klebsiella pneumonia (20%), Actinomyces pyogenes (15%), beta-hemolytic streptococci. (10%), Staphylococcus spp. (5%), and E. coli (5%). The study also revealed fungi [Penicillum spp. (5%) and Aspergillus spp. (5%)] and two calves (10%) had a yeast infection.. We conclude that florfenicol has a high bacteriological and clinical efficacy (100% and 96% respectively) in the treatment of calf respiratory tract diseases.Öğe Comparison of five methods for isolation of DNA from Mycoplasma cynos(ELSEVIER SCIENCE BV, 2017) Sakmanoglu, Ash; Sayin, Zafer; Ucan, Uckun Sait; Pinarkara, Yasemin; Uslu, Ali; Erganis, OsmanExtraction of DNA from Mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. This eventually causes cell loss during harvesting of colonies from the agar surface. Here, we used the GenElute (TM) gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting DNA from Mycoplasma. We compared the DNA extraction efficiency of the GenElute (TM) gel extraction kit from Mycoplasma cynos cultured in agar medium with four other DNA extraction methods. The results were evaluated based on the purity and amount of DNA obtained from one Mycoplasma colony. Eight strains of Mycoplasma cynos isolated from the broncho-alveolar lavage fluid of dogs were used. The GenElute (TM) gel extraction protocol was the most efficient among all the methods tested in this study as it yielded the highest amount and the purest quality of DNA (199.3 +/- 0.744 ng/mu 1) from a single colony. Among the methods tested, the GenElute (TM) gel extraction method is the most rapid, sensitive, and simple method for DNA extraction from Mycoplasma. This procedure may also prove useful for extracting DNA from other Mycoplasma species.Öğe Comparison of the efficiency of concentrated soluble recombinant phospholipase D and natural phospholipase D enzymes(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Sakmanoglu, Asli; Erganis, OsmanPhospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot (R) BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic activities were determined using the reverse CAMP test. The nucleotide sequence of the rPLD gene was 99.7% similar to the PLD gene of C. pseudotuberculosis in the NCBI GenBank Database; these differences in nucleotides resulted in a difference in two amino acids. The rPLD protein concentration and the titer of hemolytic activity were 23.1 mg/mL and 1/256, respectively. Similarities in the enzyme characteristics were detected between rPLD and dPLD enzymes. These findings indicate that a protocol would be useful for the enhanced production of soluble rPLD in E. coli and a membrane cassette system for concentration the recombinant protein.Öğe Comparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCR(UNIV ZAGREB VET FACULTY, 2016) Sayin, Zafer; Erganis, Osman; Sakmanoglu, Asli; Hadimli, Hasan H.; Pinarkara, Yasemin; Maden, Mehmet; Al Shattrawi, Huda J. G.In the present study, Pasteurella caballi (P. caballi) was isolated and identified in bronchoalveolar lavage fluid and lung samples from thoroughbred Arabian foals using conventional microbiological methods. Subsequently, the ability of two different PCR primer sets was evaluated for detection and confirmation of P. caballi. Primer sets 1 and 2, targeting the 16S rRNA gene of P. caballi, were designed using the Primer 3 and Primer-BLAST programs, respectively. PCR was performed to confirm P. caballi strains and to detect it directly in the bronchoalveolar lavage fluid and lung samples. In total, 35 Pasteurella spp. were isolated from 25 (38.4 %) of 65 bronchoalveolar lavage fluid samples, and 10 (58.8 %) of 17 lung samples. These strains were identified as P. caballi based on conventional microbiological and biochemical characteristics. The sensitivities of primers 1 and 2 were determined lobe 100 % to confirm cultured P. caballi strains. However, the specificity of P. caballi detection was lower with primer set-1 than primer set-2 in bronchoalveolar lavage fluid and lung samples. The sensitivity and specificity of primer set-2 were confirmed by gene sequence analysis. This study indicates that the 16S rRNA-PCR method, using primer set-2, provides a rapid and accurate tool for the detection and confirmation of P. caballi isolates in bronchoalveolar lavage fluid and lung samples from foals.Öğe The Effectiveness of Anti-R. equi Hyperimmune Plasma against R. equi Challenge in Thoroughbred Arabian Foals of Mares Vaccinated with R-equi Vaccine(HINDAWI LTD, 2014) Erganis, Osman; Sayin, Zafer; Hadimli, Hasan Huseyin; Sakmanoglu, Asli; Pinarkara, Yasemin; Ozdemir, Ozgur; Maden, MehmetThis study aimed to determine the effectiveness of a pregnant mare immunization of a Rhodococcus equi (R. equi) vaccine candidate containing a water-based nanoparticle mineral oil adjuvanted (Montanide IMS 3012) inactive bacterin and virulence-associated protein A (VapA), as well as the administration of anti-R. equi hyperimmune (HI) plasma against R. equi challenge in the mares' foals. The efficacy of passive immunizations (colostral passive immunity by mare vaccination and artificial passive immunity by HI plasma administration) was evaluated based on clinical signs, complete blood count, blood gas analysis, serological response (ELISA), interleukin-4 (IL-4) and interferon gamma (IFN-gamma), total cell count of the bronchoalveolar lavage fluids (BALF) samples, reisolation rate of R. equi from BALF samples (CFU/mL), lung samples (CFU/gr), and lesion scores of the organs and tissue according to pathological findings after necropsy in the foals. The vaccination of pregnant mares and HI plasma administration in the foals reduced the severity of R. equi pneumonia and lesion scores of the organs and tissue by 3.54-fold compared to the control foals. This study thus indicates that immunization of pregnant mares with R. equi vaccine candidate and administration of HI plasma in mares' foals effectively protect foals against R. equi challenge.Öğe Efficacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in mice(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2015) Erganis, Osman; Hadimli, Hasan Huseyin; Sayin, Zafer; Sakmanoglu, Asli; Pinarkara, Yasemin; Ozdemir, OzgurThe aim of this study was to determine the efficacy of inactive Rhodococcus equi vaccine candidates included that bacterin+aluminum hydroxide Al(OH)(3)), bacterin+VapA+(Al(OH)(3)), bacterin+Montanide IMS 3012 (IMS), bacterin+ VapA+IMS, and live vaccine using mice as a model. The efficacy of vaccine was evaluated according to clinical findings, humoral and cellular immunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrificed mice. Inactive R. equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injected once. Fifteen days after the last vaccination, aerosol challenges were carried out with the pathogenic R. equi VapA(+)K2002 strain in all groups. Two mice were sacrificed from each challenge groups on days 1, 3, 5, and 7. The antibody titers of vaccinated mice were found to be significantly higher than those of the controls. The largest number of INF-g positive samples were detected in the bacterin+ VapA+IMS and bacterin+ IMS groups. IL-4 positivity was determined only in live vaccine groups. The lowest reisolation rate of R. equi from internal organs was observed in the bacterin+VapA+IMS group. It was concluded that R. equi vaccines, and especially bacterin+VapA+IMS, are useful to protect mice against R. equi infection.Öğe The Enigmatical Manipulators in the Capsule Synthesis of Pasteurella Multocida: Iron Acquisition Proteins(Selçuk Üniversitesi, 2023) Balevi, Aslı; İlban, Ayşegül; Uslu, Ali; Sayın, Zafer; Gök, Ayten; Padron, Beatriz; Toslak, Eda; Erganis, OsmanAmaç: Pasteurella multocida'daki spontan kapsül kaybı veya kapsül değişiklikleri, tekrarlanan laboratuvar geçişlerinden, pozitif veya negatif düzenleyici genlerden veya bilinmeyen bir genden kaynaklanabilir. Bu çalışmada, tipik olmayan ve tipik P. multocida suşlarının fenotipik, genotipik ve biyotipik özelliklerinin karşılaştırılması, kapsül sentezindeki baskın genlerin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada kapsül tipi belirlenen 56 suş ve kapsül tipi belirlenemeyen otuz altı suş kullanıldı. İzolatlarda baskın genlerin (serogrup, serotip, toksin, adezin, demir alımı ve koruyucu) varlığına dayalı olarak çoklu doğrusal regresyon analizi kullanıldı. Bulgular: Bu suşların kültür yöntemleri ile koloni morfolojileri değerlendirildiğinde, tipik suşlarda (%87,5) mukoid koloni oluşumu, tipik olmayan suşların aksine (%27,7) yaygın olarak saptanmıştır. Tipik suşlarda en yüksek ptfA, ompA ve tadD gen yüzdeleri sırasıyla %78,57, %75 ve %69,64 idi. Tipik olmayan suşlarda en yüksek ompA, ptfA ve tadD gen oranları sırasıyla %61,1, %52,78 ve %52,78 idi. Çoklu lineer regresyon analizi sonuçlarına göre, hgbA ve hgbB genlerinin birlikteliği tipik olmayan suşlarda kapsül sentezinin artmasına neden olmuştur. Bu suşlarda ompA geninin varlığı, ikinci olarak bir indüksiyondu. Diğer genler, tipik olmayan suşlarda kapsül sentezinde etkili değildi. Öneri: Tipik olmayan P. multocida suşlarının oluşumundaki en önemli etkinin HgbA ve HgbB genlerinin yeterli olmaması ile ilgili olduğu belirlendi. P. multocida'nın demir kısıtlamalı koşullar altında yoğun bir şekilde kapsüllenmemiş olabileceği düşünüldü. Sonuç olarak, P. multocida, demir alma proteinlerine bağlı olarak kapsülünü değiştirebilir veya kapsülünü kaybedebilirÖğe Genotypic Characterization of Bordetella bronchiseptica Strains Isolated from Stray and Pet Dogs(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2016) Sayin, Zafer; Sakmanoglu, Asli; Erganis, Osman; Ucan, Uckun Sait; Hadimli, Hasan Huseyin; Aras, Zeki; Sanioglu, GokcenurBordetella bronchiseptica (B. bronchiseptica) is the most important pathogen associated with kennel cough in dogs. The presence of B. bronchiseptica in pet dogs and shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strains was determined to evaluate the role of stray dogs in spread of B. bronchiseptica to pet dogs by detection of virulence genes such as filamentous hemagglutinin (fha), pertactin (prn) and dermonecrotic toxin (dnt). We also performed the random amplified polymorphic DNA (RAPD) assay. A total of 96 B. bronchiseptica were isolated from stray and pet dogs. The fha, prn and dnt virulence genes were detected in 86, 83.3 and 61.4% strains, respectively by polymerase chain reaction (PCR) techniques. The most common genotype from stray and pet dogs was fha+prn+dnt+ as detected in 37.5% and 11.4% of all the strains, respectively. The RAPD assay showed that 3 different patterns were obtained from 96 B. bronchiseptica strains. Sixty one (63.5%) of them were clustered in one main group and then further placed in another 2 sub-groups by RAPD assay. Genetic association was seen between the B. bronchiseptica strains from stray and pet dogs. In conclusion, this study revealed that B. bronchiseptica is present at a higher rate in stray dogs than pet dogs. Stray dogs might have a significant role in the transmission of B. bronchiseptica to pet dogs. (C)2016 PVJ. All rights reservedÖğe IDENTIFICATION OF MYCOBACTERIUM STRAINS BY PCR AND PCR-REA(NATL VETERINARY RESEARCH INST, 2011) Sayin, Zafer; Erganis, OsmanThe presented study aimed isolation of Mycobacterium strains from cases of bovine tuberculosis in Turkey, and identification of these isolates by PCR and restriction endonuclease analysis (REA). Mycobacteria were isolated from bronchial, mesenteric, and prescapular lymph nodes and tubercle samples from slaughtered cattle, which were either skin-test reactors or showed tuberculous suspected lesions during meat inspection. The samples were cultured in Lowenstein-Jensen medium. Colonies suspected of being Mycobacterium sp. were analysed by the PCR method using the MTUB_c-gyrBf and MTUB_c-gyrBr primers, which amplify the 1,020-bp region of the gyrB gene, that encodes the B subunit of the DNA gyrase (topoisomerase) enzyme in strains of the Mycobacterium tuberculosis complex (MTBC). The REA of MTBC-PCR positive amplified products was performed using the Sacl1 and Rsa1 enzymes, in order to identify specific strains. The MTBC-PCR analysis of 117 bacterial strains demonstrated that all isolates belonged to the MTBC. All isolates were identified as M bovis/M bovis subsp. caprae by Rsa1-REA, whilst 93 (79.5%) of the bacterial strains were identified as M. bovis and 24 (20.4%) as M. bovis subsp. caprae by Sacll-REA. M. bovis subsp. caprae was isolated and identified for the first time in Turkey from bovine tuberculosis cases.Öğe Investigation of chicken infectious anemia virus infection by PCR and ELISA in chicken flocks(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2008) Hadimli, H. Hueseyin; Erganis, Osman; Gueler, Leyla; Ucan, U. SaitPresence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks ( 16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples from 20 flocks (80.0%) CIAV-specific DNA was detected. In commercial layers antibodies were detected in 15 (93.7%) of 16 flocks and viral DNA was detected in 13 (86.6%) of 15 flocks. In commercial broilers both CIAV antibodies and DNA were detected in 7 (70%) of 10 flocks tested. While seropositivity was detected in 12 (100%) of breeding broilers ( 8) and layer ( 4) flocks tested, no PCR was performed in these flocks. The study showed high prevalence of subclinical CIAV infection in the investigated chicken flocks.Öğe Isolation of arcanobacterium pyogenes from samples of sheep and cattle and identification by polimerase chain reaction(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2010) Hadimli, H. Hueseyin; Erganis, Osman; Kav, Kuersat; Sayin, ZaferThe aims of this study were to isolate Arcanobaterium pyogenes (A. pyogenes) from cattle and sheep, to identify the isolates by conventional methods, Polymerase Chain Reaction (PCR) and to determine antimicrobial susceptibilities. In this study, a total 716 samples was collected from sheep and cattle (liver, milk, broncho alveoler lavage, lung, suppurative tissue, and fluid of joint). All samples were cultured onto blood agar base containing 5% defibrinated sheep blood and plates were incubated at 37 degrees C for 48 h. Total 51 A. pyogenes strains isolated from samples were identified in according to properties of biochemical. Then, PCR analyses were made using specific primers for A. pyogenes. All isolates were also confirmed by PCR. In addition, antimicrobial activities were determined to 16 antimicrobial agents, some of which were used in veterinary medicine. There is emphasized that this bacterium may cause a variety of infections with economically losses in sheep and cattle.Öğe Production and development of vaccines for ornithobacterium rhinotracheale infection in turkeys(2010) Erganis, Osman; Hadimli, Hasan Hüseyin; Kav, Kursat; Sayin, Zafer; Aras, ZekiErganiş O, Hadimli HH, Kav K, Sayın Z, Aras Z. Hindilerde Ornithobacterium rhinotracheale için aşıların geliştirilmesi ve üretilmesi. Eurasian J Vet Sci, 2010, 26, 2, 101-107 Amaç: Bu çalışmanın amacı bivalan inaktif Ornithobacterium rhinotracheale aşıları hazırlamak, kan serumlarında antijenlere karşı antikorların titrelerini ölçmek ve hindilerde O. rhinotracheale aşılarının etkinliklerini belirlemektir. Gereç ve Yöntem: Bivalan inaktif O. rhinotracheale aşıları; alüminyum hidroksit, mineral yağlı, alüminyum hidroksit ginseng ve mineral yağ ginseng adjuvantları kullanılarak O. rhinotracheale serotip A ve B’den hazırlandı. Sterilite ve zararsızlık testlerinden sonra, hindilerde (5. ve 8. haftalarda 0.25ml ve 0.5 ml dozlarla iki kez aşılama ile) aşıların laboratuvar etkinlikleri (çelınç/koruma ve serolojik potens) yapıldı. Bulgular: Çelınç sonuçlarına göre hindilerde bütün aşıların %100 etkili olduğu bulundu. Aşılı ve aşısız grupların serumlarında titrelerin serolojik ölçümleri için ve saha şartlarında O. rhinotracheale enfeksiyonunun teşhisinde lam aglütinasyon, mikro serum aglütinasyon ve ELISA testleri kullanıldı. Adjuvant olarak mineral yağ ve ginseng içeren aşı diğerlerine göre belirgin olarak daha yüksek humoral immune cevap oluşturdu. Aynı zamanda ve mineral yağ ginseng aşısı özel bir hindi işletmesinde saha denemesinde çok etkili olduğu belirlendi. Öneri: Kanatlılarda ornitobakteriozisin önlenmesi için O. rhinotracheale aşıları kullanılabilir.Öğe Production and development of vaccines for Ornithobacterium rhinotracheale infection in turkeys(Selçuk Üniversitesi Veterinerlik Fakültesi, 2010) Erganis, Osman; Kav, Kursat; Aras, ZekiAmaç: Bu çalışmanın amacı bivalan inaktif Ornithobacterium rhinotracheale aşıları hazırlamak, kan serumlarında antijenlere karşı antikorların titrelerini ölçmek ve hindilerde O. rhinotracheale aşılarının etkinliklerini belirlemektir. Gereç ve Yöntem: Bivalan inaktif O. rhinotracheale aşıları; alüminyum hidroksit, mineral yağlı, alüminyum hidroksit + ginseng ve mineral yağ + ginseng adjuvantları kullanılarak O. rhinotracheale serotip A ve B’den hazırlandı. Sterilite ve zararsızlık testlerinden sonra, hindilerde (5. ve 8. haftalarda 0.25ml ve 0.5 ml dozlarla iki kez aşılama ile) aşıların laboratuvar etkinlikleri (çelınç/koruma ve serolojik potens) yapıldı. Bulgular: Çelınç sonuçlarına göre hindilerde bütün aşıların %100 etkili olduğu bulundu. Aşılı ve aşısız grupların serumlarında titrelerin serolojik ölçümleri için ve saha şartlarında O. rhinotracheale enfeksiyonunun teşhisinde lam aglütinasyon, mikro serum aglütinasyon ve ELISA testleri kullanıldı. Adjuvant olarak mineral yağ ve ginseng içeren aşı diğerlerine göre belirgin olarak daha yüksek humoral immune cevap oluşturdu. Aynı zamanda ve mineral yağ + ginseng aşısı özel bir hindi işletmesinde saha denemesinde çok etkili olduğu belirlendi. Öneri: Kanatlılarda ornitobakteriozisin önlenmesi için O. rhinotracheale aşıları kullanılabilir.Öğe Sero-Epidemiology of the Rhodococcus Equi in Horses in Eastern Kazakhstan(Selçuk Üniversitesi, 2023 Haziran) Uslu, Ali; Sayin, Zafer; Balevi, Aslı; Ilban, Aysegul; Erganis, Osman; Yerken, Kassymov; Bauyrzhan, Otarbayev; Yerbol, Ismagulov; Gulnaz, IlgekbayevaAmaç: Bu çalışma Doğu Kazakistan'daki yetişkin atlarda Rhodococcus equi (R. equi) sero-prevalansını değerlendirmek için tasarlanmıştır. Gereç ve Yöntem: Eylül ve Kasım 2021 tarihleri arasında doğu Kazakistan’da bulunan üç bölgede (Zhambyl, Almatı, Doğu Kazakistan) 311 attan serum toplandı ve virülent R. equi'ye karşı oluşmuş olan antikorların varlığı enzim bağıntılı immünosorbent test (ELISA) ile tespit edildi. Bulgular: R. equi'nin doğu Kazakistan’da bulunan at popüslayonlarında ki seroprevalansı 2021 yılında %93,6 olarak bulundu. En yüksek seroprevalans Vap A oranı Zhambly Bölgesi'nde (%98,02), en düşük ise Doğu Kazakistan Bölgesi'nde (%88,89) görüldü. Bu bölgeden alınan örnekler toplam örneklerin %69,8'ini oluşturmakta olup, %3,84'ü vapA seronegatiftir. R. equi’nin Doğu Kazakistan'da endemik enfeksiyona sebep olduğu tespit edildi.. Öneri: Bu, R. equi'nin Kazakistan'da epidemiyolojisi hakkında bilgi sağlayan ilk sero survey çalışmasıdır. Serolojik bulgular klinik vakalar ve tay tavlalarında bakteri izolasyonu ile desteklenmelidir. Doğu Kazakistan'da R. equi'ye karşı koruma ve kontrol programları uygulanmalıdır.Öğe Serotypes of Salmonella isolated from feces of cattle, buffalo, and camel and sensitivities to antibiotics in Turkey(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2017) Hadimli, Hasan Huseyin; Pinarkara, Yasemin; Sakmanoglu, Asli; Sayin, Zafer; Erganis, Osman; Uslu, Ali; Al-Shattrawi, Huda JihadIn this study, Salmonella strains from dairy cows, calves with diarrhea, and buffalo and camel feces were isolated and serotyped. There were 869 feces samples (437 calves, 287 dairy cows, 100 buffalo, and 45 camels) collected from 13 provinces and 21 farms. Preenrichment feces samples were added to Rappaport-Vassiliadis medium. Samples were then subcultured on xylose lysine tergitol-4 agar and plates. Suspected colonies of Salmonella were confirmed by latex agglutination test. The isolates of Salmonella spp. were serotyped at the Etlik Central Veterinary Control Institute. In total, 40 Salmonella spp. were isolated, including 33 from calves, 5 from dairy cows, 1 from a buffalo, and 1 from a camel. Salmonella strains were serotyped as S. Kentucky (n = 23), S. Muenchen (n = 5), S. Anatum (n = 4), S. Gaminare (n = 4), S. Typhimurium (n = 1), S. Muenster (n = 1), S. Enteritidis (n = 1), and S. Abony (n = 1). The Kirby-Bauer disk diffusion method was used for the determination of antibiotic susceptibilities of isolates against 15 antibiotics. Salmonella isolates were determined as resistant to multiple antibiotics. In conclusion, 8 different Salmonella serotypes were found in dairy cows, calves, buffalo, and camels. Because of the occurrence of multiresistant isolates, biosafety measures and pathogen control processes are advised for Salmonella-associated risks to public health.