Isolation of arcanobacterium pyogenes from samples of sheep and cattle and identification by polimerase chain reaction
Yükleniyor...
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
KAFKAS UNIV, VETERINER FAKULTESI DERGISI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aims of this study were to isolate Arcanobaterium pyogenes (A. pyogenes) from cattle and sheep, to identify the isolates by conventional methods, Polymerase Chain Reaction (PCR) and to determine antimicrobial susceptibilities. In this study, a total 716 samples was collected from sheep and cattle (liver, milk, broncho alveoler lavage, lung, suppurative tissue, and fluid of joint). All samples were cultured onto blood agar base containing 5% defibrinated sheep blood and plates were incubated at 37 degrees C for 48 h. Total 51 A. pyogenes strains isolated from samples were identified in according to properties of biochemical. Then, PCR analyses were made using specific primers for A. pyogenes. All isolates were also confirmed by PCR. In addition, antimicrobial activities were determined to 16 antimicrobial agents, some of which were used in veterinary medicine. There is emphasized that this bacterium may cause a variety of infections with economically losses in sheep and cattle.
Açıklama
Anahtar Kelimeler
Arcanobacterium pyogenes, Cattle, Sheep, Milk, Liver, Lung, Bronco-alveolar lavege
Kaynak
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
16
Sayı
4
Künye
Hadimli, H. H., Erganiş, O., Kav, K., Sayın, Z. (2010). Isolation of Arcanobacterium Pyogenes from Samples of Sheep and Cattle and Identification by Polimerase Chain Reaction. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(4), 611-616.
