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  • Küçük Resim
    Öğe
    The Anticancer Activity of Juglone Via Inducing Programmed Cell Death In Pancreatic Cancer Cells
    (Selçuk Üniversitesi, 2023 Eylül) Erkoç Kaya, Dudu; Dursunoğlu, Duygu
    Purpose: Pancreatic cancer (PC), has a poor prognosis with its silent course until the advanced level without any symptoms. Additionally, currently limited treatment options makes developing alternative treatment strategies and agents mandatory. In researches conducted to investigate new therapeutic agents for cancer, natural products are main sources. Among all, Juglone, a natural naphthoquinone, stands out with its cytotoxic effects in cancer cells, induction of apoptosis and autophagy, inhibition of antiangiogenesis and migration. Thus, in this study, we aimed to investigate the anticancer activity and the apoptotic effects of juglone in BxPC-3 pancreatic cancer cells. Methods: BxPC-3 pancreatic cancer cells were treated with different doses of juglone. To evaluate the apoptotic effects following juglone treatments; proapoptotic Bax, antiapoptotic Bcl-2 and an important inhibitor of apoptosis Birc5 (Survivin) gene expressions were determined by qPCR analysis. We also confirmed the results for apoptosis by performing immunofluorescence analysis using Annexin V-FITC. Results: According to qPCR analysis, juglone induced statistically significant 2.24, 1.57, 2.43 fold increases of Bax gene expressions at 10, 15 and 20µM doses, respectively. We detected low fold increases after juglone treatments for Bcl-2 gene expression at other treatments except 20µM treatment. These changes were not statistically significant. Juglone decreased survivin gene expression at all treatment doses while 2.39 fold decrease at 20?M doses of juglone was statistically significant. Also, we confirmed clearly by the immunofluorescence analysis that juglone increased apoptosis dose-dependently in BxPC-3 cells. Conclusion: Taken together all the results of gene expression and immunfloresence analysis, our study suggests that juglone shows anticancer activity by inducing apoptosis possibly enhancing intrinsic apoptotic pathway in pancreatic cancer cells.
  • Küçük Resim
    Öğe
    Juglone Effects by Dual Way on mTOR Gene Expression, Which Plays Central Role in Cell Growth, Survival and Metabolism, in PANC-1 and BxPC-3 Pancreatic Cancer Cell Lines
    (Selçuk Üniversitesi, 2023) Demirbaş Büyüktüt, Emine Merve; Erkoç Kaya, Dudu; Göktürk, Fatma; Arıkoğlu, Hilal
    Background/Aims: Juglone, as a naphthoquinone, has been shown to have cytotoxic and apoptotic effects in various cancer cells and besides this effects it was reported to have antiinvasive and anti-metastatic effects in PANC-1 and BxPC-3 cells in our previous studies. In this study, we investigated the effects of juglone on GRP75, TFAM and mTOR genes encoding key proteins associated with mitochondrial biogenesis and activation in PANC-1 and BxPC-3 pancreatic cancer cells since mitochondria have central roles in cancer cell survival, metastasis and therapeutic resistance. Methods: In our study; 5, 10, 15 and 20 ?M juglone doses were selected as the application doses considering the IC50 value determined after MTT test results and the expressions of the target genes were analyzed by qPCR method after application of juglone doses for 24 hours. Results: Our study results revealed that juglone had an opposite and strong effects on mTOR expression in both cell lines. Conclusion: Our findings suggest that juglone has a developable potential and is a promising theurapeutic agent to develop new strategies for the battle with cancer with those effects on mTOR gene which plays a central role in cellular homeostasis and several cellular events including cell growth, survival and metabolism.