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  • Küçük Resim Yok
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    Colistin resistance in carbapenem-resistant Klebsiella pneumoniae strains
    (Scientific Publishers of India, 2016) Kalem F.; Ergun A.G.; Ertuğrul Ö.; Özçimen S.; Şimşek H.; Süzük S.; Ünaldı Ö.
    Objective: Because of the increase in the infections caused by carbapenem-resistant Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae; nowadays colistin is used more frequently. In this study, the firstly detected colistin resistance in carbapenem-resistant KPC-producing K. pneumoniae strains were evaluated. Material and methods: For identification and susceptibility testing; VITEK 2 Compact (bioMérieux, France) have been used. Because of resistance; MICs were studied with gradient test method in Microbiology Reference Laboratory, Public Health Institution of Turkey, Ministry of Health, Ankara, Turkey for confirmation. The presence of carbapenem resistance genes (OXA23, NDM1, OXA48, KPC, VIM ve IMP) was investigated by Polymerase Chain Reaction (PCR) method. Pulsed Field Gel Electrophoresis (PFGE) method was used to determine the clonal relationships between strains. PCR and PFGE tests have been studied in Molecular Microbiology Research and Application Laboratory Department of Microbiology Reference Laboratories, Public Health Institution of Turkey, Ministry of Health, Ankara, Turkey. Results: All strains were resistance for carbapenems and colistin Two of four strains were isolated from patients hospitalized in intensive care and two of them were isolated from patients hospitalized in clinics. Resistance to carbapenems were confirmed genotypically. Two strains isolated from patients in clinics were positive for NDM1 and OX-48, and isolates from patients in intensive care unit were positive for only OXA-48 carbapenem genes. PFGE typing method described two clones that have a relationship with each other. The strains in which NDM1 and OXA-48 were together positive were in one clone and OXA-48-positive strains were in other clone. Conclusion: The emergence of colistin resistant strains is a very important problem due to decrease of treatment options for infections caused by carbapenem-resistant KPC producing K. pneumoniae. Colistin should not be used alone, combination therapy should be preferred. © 2016, Scientific Publishers of India. All rights reserved.
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    Comparison of two specific Ig E test in detection of allergen in asthma disease with referance of prick test
    (2008) Özdemir M.; Kalem F.; Girgiç M.; Kalem A.C.; Baysal B.
    Objective: It is aimed to compare two specific methods of allergen tests in patients who have diagnosed as asthma with history, physical examination and breath function test and to evaluate the results by comparison with prick test as a standart in this study. Method: In this study serum samples had taken from 52 asthmatic patients. Using both spesific IgE RAST and UniCAP System FEIA; the specific IgE titres have been measured. Then the results have been compared statistically. Results: Sixty one parameters were positive with prick test. RAST method showed positive results for 46 parameters. 23 parameters were positive with UniCAP System. Conclusion: These results showed that RAST method shows better correlation with prick test than UniCAP System. In asthmatic patients; skin prick test and measurement of specific IgE levels are useful for detection of allergen. In asthmatic patients; if skin prick test and spesifik IgE levels gave similiar results, it could be possible to say that this allergen is the agent of allergic disease.
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    Investigation of viral pathogens during pregnancy in a city region in Turkey [Türki?ye'ni?n bi?r şehri?nde gebeli?k sirasinda vi?ral patojenleri?n araştirilmasi]
    (2011) Özdemir M.; Kalem F.; Feyzio?lu B.; Baysal B.
    Rubella virus, Cytomegalovirus (CMV) and Herpes simplex virus (HSV)-II are the infectious agents which may cause congenital malformations in the fetus if acquired during pregnancy. Thus these agents must be screened throughout pregnancy. This screening is also important to demonstrate the regional status regarding these agents. In this study; it is aimed to evaluate the seroprevalance of Rubella, CMV and HSV-II in pregnant women in a city region in Turkey. A total of 249 pregnant women who applied to the outpatient clinics of the obstetric and gynaecological department of a university hospital were involved to the study during antenatal screening in the first trimester of pregnancy. After separation of blood sample sera samples were tested for antibodies to Rubella, CMV and HSV-II. While Rubella and CMV antibodies were tested using Enzyme Linked Floeresan Antibody method, HSV-II was tested using ELISA. The mean age of the participants in this study was 28.4± 3.4 years. The seropositivity for anti-Rubella IgM, anti-Rubella IgG, anti-CMV IgM, anti-CMV IgG, anti-HSV-II IgM, and anti-HSV-II IgG were 0.4%, 95.9%, 0%, 98.7%, 0%, 4.4%, respectively. Although the seropositivity of HSV-II was very low, seropositivities of Rubella and CMV were very high in our area of investigation. Viruses causing congenital infection and malformation must be screened in pregnant women individually and avidity tests must be applied in suspicious cases. In addition the seroprevelance of these viral infections should be known in every population in order to take preventive measures and prepare local and general health policies.