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Öğe Biocompatible yogurt carbon dots: evaluation of utilization for medical applications(SPRINGER, 2017) Dinç, Saliha; Kara, Meryem; Kars, Meltem Demirel; Aykul, Fatmanur; Çiçekci, Hacer; Akkuş, MehmetIn this study, carbon dots (CDs) were produced from yogurt, a fermented milk product, via microwave-assisted process (800 W) in 30 min without using any additional chemical agents. Yogurt CDs had outstanding nitrogen and oxygen ratios. These dots were monodisperse and about 2 nm sized. The toxicological assessments of yogurt carbon dots in human cancer cells and normal epithelial cells and their fluorescence imaging in living cell system were carried out. Yogurt carbon dots had intense fluorescent signal under confocal microscopy and good fluorescence stability in living cell system. The resulting yogurt carbon dots exhibited high biocompatibility up to 7.1 mg/mL CD concentration which may find utilization in medical applications such as cellular tracking, imaging and drug delivery. Yogurt carbon dots have potential to be good diagnostic agents to visualize cancer cells which may be developed as a therapeutic carrier.Öğe Comparative Study of Chemical and Biochemical Properties of Different Melon Cultivars: Standard, Hybrid, and Grafted Melons(Amer Chemical Soc, 2010) Kolaylı, Sevgi; Kara, Meryem; Tezcan, Filiz; Erim, F. Bedia; Şahin, Hüseyin; Ulusoy, Esra; Aliyazıcıoğlu, RezzanChemical and biochemical properties of standard, hybrid, and grafted melons cultivated under the same agricultural conditions in adjacent fields in the Cumra region of Turkey were investigated and compared based on pH, Brix, antioxidant activity, total phenolics, ascorbic acid, individual phenolics, sugar, and organic acid values. Seventeen different phenolic constituents were quantified by reverse phase high-performance liquid chromatography (RP-HPLC). The highest phenolic acid variability and content were detected in the standard melon. Sugar and organic acid compositions of melon cultivars were tested by capillary electrophoresis, and significant differences in types and contents of individual sugars and organic acids were determined among the cultivars. Standard Cinikiz Cumra melons had the highest ascorbic acid, total phenolics, and total sugar contents. The fructose/glucose ratio increased three times in grafted melon as compared with standard melon. While sugar alcohol mannitol existed in the standard and hybrid cultivars, this constituent disappeared in the grafted types. Citric acid found in the standard cultivar was not detected in the hybrid and grafted types. Consequently, it was concluded that the nutritional value of melons changed by the application of hybridization, grafting, or standard (open pollinated) production methods. The standard melon was found to have the highest score in terms of taste, because of its highest sweetness and sourness. It was also found preferable because of its high antioxidant activity, total phenolic and ascorbic acid contents.Öğe Comparison of Biochemical Parameters and Volatile Compounds of Some Hybrid Tomato Varieties(ANKARA UNIV, FAC AGR, 2017) Kara, Meryem; Sahin, Huseyin; Dinc, Saliha; Yildiz, Oktay; Can, Zehra; Er, FatihEvaluating morphological and quality parameters such as shape, colour, size and yield are all common practices when assessing the quality of fruit and vegetables. In recent times, however, consumer interest in foods with high levels of bioactive components, high mineral content and acceptable flavor has increased considerably. In order to make a contribution to the production of fruit and vegetables with excellent morphological properties and high levels of bioactive compounds, two different hybrid tomato varieties (Ege F1 and Alida F1) cultivated in Turkey were compared in terms of their biochemical parameters (phenolic compounds, ascorbic acid, ss-carotene, lycopene, minerals) and volatiles. While the Alida F1 was rated the highest for lycopene and mineral values, the Ege F1 was outstanding in terms of phenolic compounds and volatiles. Ascorbic acid content of both cultivars ranged from 7.13 to 11.94 mg 100 g(-1) fw. The quantity in the Ege hybrid was remarkable. Chlorogenic acid, caffeic acid, p-coumaric acid, rutin and trans ferulic acid were the main phenolic compounds in both cultivars. Potassium (K) (4.09%) was the most predominant mineral in both hybrid tomatoes. Hexenal had the highest concentration value among tested volatiles followed by 2-hexenal, (6-methyl)-trans-5-Hepten-2-one. Comparison of biochemical parameters and volatiles of both varieties will give breeders an opportunity to choose the desired traits of tomatoes to cultivate for both taste and nutritional value.Öğe Determination of Chemical, Physical and Biological Characteristics of Some Pekmez (Molasses) From Turkey(2009) Aliyazıcıoğlu, Rezzan; Kolaylı, Sevgi; Kara, Meryem; Yıldız, Oktay; Sarıkaya, Ali Osman; Cengiz, Sevil; Er, FatihAntioxidant capacity, chemical and physical properties of 6 certificated and 1 uncertificated pekmez (molasses) samples of Turkish varieties were analyzed. All pekmez samples had similar chemical compositions. Total phenolic contents varied from 138 to 243 mg of gallic acid equivalents/100 g samples. The apricot pekmez had the highest phenolic content (243 ± 16), while the carob pekmez exhibited the highest DPPH (0.08 ± 0.01 mg g-1) radical scavenging activity. DPPH radical scavenging activity was also found to be related to concentrations of the samples. Especially, selenium and other minerals content were found slightly higher in grape pekmez than the others. Present results showed that all the certificated and uncertificated pekmez samples had no any risk in public health and besides their high content of sugar. Each pekmez had antioxidant and high amount of polyphenols and minerals.Öğe Determination of homogenization performance of grinder used for aflatoxin analyses of Turkish hazelnuts(2009) Baltacı, Cemalettin; Kara, Meryem; Aliyazıcıoğlu, Rezzan; Kolaylı, Sevgi; Karaman, Mustafa; Yıldız, OktayThis research was conducted to determine the homogeneity performance of laboratory grinder used for aflatoxin (Aflatoxin B1: AFB1; Aflatoxin B2: AFB2; Aflatoxin G1: AFG1; Aflatoxin G2: AFG2) analysis of hazelnut, one of the strategic products from the Black Sea Region. For the research, naturally aflatoxins-contaminated hazelnut samples in their unshelled state (A) and non-contaminated unshelled hazelnut samples (B) were used. Hazelnut samples in which aflatoxin (AFs) couldn't be detected by the analytical procedure were treated with standard solutions of 10 pg mL-1 AFB1, 5 pg mL"1 AFB2, 10 pg mL"1 AFG1, 5 pg mL"1 AFG2 at the levels of 2 mL, 0.6 mL, 4 mL and 1 mL, respectively. Then, hazelnut samples treated with AFs were grounded in laboratory grinders after 30 min of resting. The Cochran test procedure was applied to duplicate results to determine sufficient homogeneity of grinder. Variations among samples in both hazelnut groups were smaller than critical value of the statistical procedure of the Cochran test.Öğe THE EFFECT OF DIFFERENT MANURES AND SYNTHETIC FERTILIZER ON BIOCHEMICAL AND ANTIMICROBIAL PROPERTIES OF MENTHA PIPERITA L(WILEY, 2014) Ozbucak, Tugba Bayrak; Erturk, Omer; Yildiz, Oktay; Bayrak, Ali; Kara, Meryem; Sahin, Huseyin; Kiralan, MustafaThe study was designed to examine the effects of different manures (fish, pigeon and cow) and synthetic fertilizer (nitrogen) on some biochemical activities of Mentha piperita L. (mint, peppermint). Seventeen different phenolic constituents and 19 essential oils were determined in M. piperita samples. While caffeic, gallic, ferulic, protocatechuic, syringic, o-coumaric acids and rutin were detected as common phenolics; carvone, limonene and 1.8-cineol were identified as major essential oil components in all mints. Total phenolic compounds, ferric reducing antioxidant power and cupric (III) reducing capacity (CUPRAC) tests were used to evaluate antioxidant capacities of the mints. The highest total phenolic compound (4.8 mg gallic acid equivalents/100 g) and antioxidant capacity (971 mM Fe(II)/mg; 823 mM Trolox /100 g) were observed in mints fertilized with pigeon manure. Mint samples also had different antimicrobial activities against the studied microorganisms (eight bacteria and two fungi), especially Escherichia coli and Listeria monocytogenes.Öğe THE EFFECT OF DIFFERENT WAYS AND DOSAGES OF BORON APPLICATION ON BLACK DIMRIT (Vitis vinifera L.) GRAPE'S YIELD AND QUALITY(SCIENTIFIC ISSUES NATL CENTRE AGRARIAN SCIENCES, 2011) Er, F.; Akın, A.; Kara, MeryemThis study has been carried out to determine the effects of Boron on the yield and quality of Black Dimrit grape Hadim-Aladag (Konya) when NPK is applied and not applied as ground fertilizer. The experiment was carried out in N0P0K0 not applied and NPK applied 150-50-50 g/vine stocks (N1P1K1) by giving Boron as 11% Borax dosages; B-0 0 g, B-1 2,5 g, B-2 5 g, B-3 10 g Boron/vinestock. The Boron application: I. Boron application was on vinestock drop-lines by mixing it (20-30 cm deep) 15 days before blooming, II. Application started 15 days before blooming and repeated at 15 day intervals. One fourth of boron was applied by spraying on the leaves in four times.Öğe Effect of pentosanase on dough and bread properties produced by different types of flours(BLACKWELL PUBLISHING, 2008) Koyuncu, Oezkan; Bilgicli, Nermin; Elgun, Adem; Kara, MeryemThe effects of pentosanase at different doses (20, 60 and 100 ppm) on physical dough properties and bread quality were studied using three types of wheat flours. Flour A was a regular bread flour, flour B had a high hardness ratio and protein content, and flour C was prepared from the same blend of flour A but had a high extraction ratio. Regarding farinograph data, water absorption values of the high extraction (86%) flour C and high hardness (65%) blend flour B increased with introduction of pentosanase. Extensibility values of the flours increased moderately with pentosanase addition, while resistance and energy values decreased. The volume of breads made with flours C and B decreased upon addition of pentosanase. But loaf volume of breads prepared with regular bread flour A with 50% hardness and 76% extraction rate increased with high levels of pentosanase addition. In conclusion, flour A as a regular bread flour gave satisfactory results with pentosanase supplementations, whereas the harder-blend (65%) and higher-extraction-rate (85%) flours from the same cultivars did not.Öğe Ground Urfa isot peppers with high content of bioactive compounds and antioxidant activity(AGRIMEDIA GMBH, 2015) Kara, Meryem; Dinç, Saliha; Kahraman, Selma; Karıpçin, M. Zeki; Alp, İrem; Çiçekci, HacerUrfa isot pepper is one of the most important spices in the diet of Turkish people; particularly of those in the Sanliurfa region. This is the first study reporting the bioactive compounds (beta-carotene, lycopene, ascorbic acid, phenolic compounds, capsaicin) and antioxidant activity of ground Urfa isot peppers produced both at homestads and factories. Factory-made red-blackish isot pepper showed the highest DPPH radical-scavenging activity, followed by homemade red-blackish isot pepper and homemade red isot pepper. FRAP values of all Urfa isot peppers were statistically in the same group. Homemade red isot pepper had the highest total phenolic content, lycopene and beta-carotene. The capsaicin content of ground Urfa isot peppers varied from 0.15-1.2 mg/g of pepper. The homemade red-blackish ground pepper had the highest ascorbic acid level. Phenolics of peppers were remarkable and higher than those reported in the literature. With their high number of phenolic constituents, as well as other bioactive compounds, ground Urfa isot peppers can be considered an important nutritional and functional food resource.Öğe High temperature-tolerant tomato lines: bioactive compounds(SPRINGER INTERNATIONAL PUBLISHING AG, 2016) Karıpçin, M. Zeki; Dinç, Saliha; Kara, Meryem; Kahraman, Selma; Alp, İrem; Çiçekci, HacerHigh global temperatures will be a great challenge for agricultural production in the near future. Therefore, adaptation of agricultural crops to the high temperatures or development of new heat-tolerant crops with known bioactive compounds is an important strategy for nutritional health and crop improvement programs. In this context, the bioactive compounds of newly developed high temperature-tolerant tomato lines were investigated in terms of lycopene, beta-carotene, ascorbic acid and phenolic compounds to assist breeders for the selection of high temperature tolerant tomato varieties with high nutritional quality. Nineteen tomato lines, including control varieties from the "Turkey F1 Hybrid vegetable variety and quality line development" project and the GAP Agricultural Research Institute, were selected for the investigation. Line 11 was superior in lycopene and beta-carotene content, while line 18 was superior in ascorbic acid content as compared to control varieties Cansun F1 and Alsancak RN F1. Urfa 2012 and line 28 were found to have the highest numbers of phenolic compounds. Chlorogenic acid, naringenin, trans-ferulic acid and rutin were key phenolic compounds detected in each of the tomato lines. These newly released tomato lines can be used as value-added germplasm or parent sources to develop new heat-tolerant cultivars in breeding programs or as commercial cultivars for their superior functional qualities.Öğe AN INVESTIGATION OF FREQUENTLY CONSUMED EDIBLE OILS IN TURKEY IN TERMS OF OMEGA FATTY ACIDS(SPRINGER, 2011) Kolaylı, Sevgi; Tarhan, Özlem; Kara, Meryem; Aliyazıcıoğlu, Rezzan; Küçük, MuratThe fatty acid profiles of frequently consumed oils and crops cultivated in Turkey were investigated in regard to omega fatty acids. Analyses were carried out on commercially sold oils, sunflower, olive, and fish oils, and oils extracted from fatty seeds of hazelnut, walnut, olive, sunflower, poppy, sesame, and pumpkin, and butter produced in Turkey. Hazelnut and olive oils were found to be rich in omega-9 (oleic acid 18:1), walnut, poppy seed, sesame, and pumpkin seed were rich in omega-6 (linoleic acid 18:2), and butter was rich in short chain fatty acids and omega-9. Fish oil, from mackerel, was the richest in omega-3 fatty acids and fatty acid diversity. There were some alterations between commercially sold oils and oils extracted from seeds in regard to fatty acid percentages and variety.Öğe Production and some properties of traditional sugar beet pekmez from Turkey(INT SUGAR JOURNAL LTD, 2015) Yildiz, Oktay; Kara, Meryem; Dinc, SalihaSugar beet pekmez is a traditional food in Turkey commonly produced in the sugar beet farming region. This traditional food was analyzed for its physicochemical properties, mineral content, antioxidant capacity, phenolic compounds and rheological properties. Total soluble content and HMF content of pekmez were 78.2 degrees Brix and 97.45 mg/kg, respectively. pH of sugar beet pekmez (4.65) was lower than Turkish Standard (5 <= 3H<6) but this value was in agreement with that of sour liquid pekmez.. Sugar beet pekmez's acidity and total protein content were 0.672 % and 1.80 % respectively. Sucrose content of pekmez at 29.3% was highest, followed by invert sugars (15.5%). Sodium and potassium were the main minerals. Antioxidant capacity of pekmez was remarkable. Total phenolic content, flavanoid content, FRAP value and DPPH activity of pekmez were 1.850 mg GAE/g, 0.013 mg QE/g, 11.245 mmol TE/g and 5.108 mg/mL, respectively. Eleven phenolic compounds were analyzed and six phenolic compounds, catechin, p-coumaric acid, cinnamic acid, rutin, protocatechuic acid and quercetin were quantified. p-coumaric acid (126.29 mg /kg), rutin (89.89 mg/kg), quercetin (75 mg/kg) and catechin (66.57 mg/kg) were found at high concentrations in the pekmez. As for rheological analysis, sugar beet pekmez exhibited pseudoplastic behavior. Sugar beet pekmez can be regarded as a new nutritive product for consumers and producers.Öğe Promising Strain of Acinetobacter from Soil for Utilization of Gluconic Acid Production(2017) Dinç, Saliha; Kara, Meryem; Öğüt, Mehmet; Er, Fatih; Çiçekçi, HacerGluconic acid, a food additive, is used in many foods to control acidity or binds metals such as calcium, iron. Acinetobacter sp. WR326, newly isolated from soil possesses high phosphate solubilizing activity and do not require pyrroloquinoline quinone (PQQ) for glucose dehydrogenase (GDH) activity as cofactor In this study the gluconic acid production potential of this bacterium was investigated. Firstly, Acinetobacter sp. WR326 was incubated in tricalcium phosphate medium (TCP) with varying glucose concentrations (100, 250, 500 mM), at a temperature of 30C for 5 days (120 hours). The highest gluconic acid yield (59%) was found at a glucose concentration of 100 mM. Then three different levels of gluconic acid addition to the medium (50, 100, 200 mM) were tested. When Acinetobacter sp. WR326 strain was cultivated with a 100 mM glucose and 100 mM gluconic acid the yield increased to 95.27%. In any trials 2 -keto D-gluconic acid, causes problems in processing and purification of the gluconic acid, was not detected in the medium. As a conclusion, Acinetobacter sp. WR326 may be considered novel potential bacterial strain for gluconic acid production.Öğe THE ROOTSTOCK EFFECTS ON AGRONOMIC AND BIOCHEMICAL QUALITY PROPERTIES OF MELON UNDER WATER STRESS(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2018) Dinç, Saliha; Kara, Meryem; Karipcin, M. Zeki; Sarı, Nebahat; Can, Zehra; Çiçekci, Hacer; Akkuş, MehmetIn this study Edali F1 and Balhan Fl melons (Cucumis melo L.) grafted onto different rootstocks (TZ 148 and Jumbo) under water stress conditions (W1-100%, W2-50%, W3-25%). FRAP (Ferric reducing antioxidant power) values increased with the use of TZ 148 and Jumbo rootstocks. At water stress, grafting generally increased ascorbic acid values. Maximum beta-carotene amount was stated for Balhan Fl with W3 level and the beta-carotene values of Balhan/TZ148 melons increased with water stress. p-hydroxybenzoic acid was the compound with the highest quantity among the tested phenolic compounds. Citric, maleic, fumaric and formic acid quantities increased with enhancement of water deficiency whereas tartaric, succinic and acetic acid decreased. Fructose and saccharose quantity decreased as the amount of water given to plant decreased but glucose level was not affected. Consequently, grafting of melons onto favorable rootstocks seems to enhance the amount of health related compounds under water stress conditions.Öğe Utilization of chloramphenicol imprinted nanoparticles in surface plasmon resonance nanosensors(ELSEVIER SCIENCE BV, 2016) Kara, Meryem; Uzun, Lokman; Kolayli, Sevgi; Denizli, Adil[Abstract not Available]
