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Öğe A Comparative Study on Detection of Ornithobacterium Rhinotracheale Antibodies in Meat-type Turkeys by Dot Immunobinding Assay, Rapid Agglutination Test and Serum Agglutination Test(Carfax Publishing, 2002) Erganiş, Osman; Hadimli, Hasan Hüseyin; Kav, K.; Çorlu, M.; Öztürk, D.The objectives of the present study were to develop a dot-immunobinding assay (DIA) and a serum agglutination test (SAT) for detection of Ornithobacterium rhinotracheale, to compare the rapid agglutination test (RAT) and the SAT, and to make a serosurvey of O. rhinotracheale exposure on turkey farms in Turkey. Antiserum against O. rhinotracheale bacterin was prepared in rabbits and 72 serum samples were collected from turkeys with respiratory signs on four farms. Comparison of the tests showed that 55.5, 48.6 and 40.3% of serum samples were positive by RAT, SAT and DIA, respectively. The sensitivity of the DIA appeared to be lower than that of the agglutination tests but the specificity is not known.Öğe Comparison of Antibacterial Activity of Two Dentin Bonding Systems Using Agar Well Technique and Tooth Cavity Model(Elsevier Sci Ltd, 2003) Özer, F.; Karakaya, S.; Ünlü, Nimet; Erganiş, Osman; Kav, K.; Imazato, S.Objective. This study compared the antibacterial activities of two dentin bonding systems (ABF, Kuraray and Reactmer Bond, Shofu) by a conventional agar well technique and a newly designed in vitro test using tooth model. Methods. In the agar well technique, the test materials were filled in the welts of Muller Hinton agar plates inoculated with Streptococcus mutans NCTC10449, and the diameters of inhibition zones produced around the materials were measured after 24 h of incubation. For the tooth model test, three cavities (diameter 1 mm, depth 2 mm) were prepared in the flat occlusal dentin of human extracted molar. After sterilization, the teeth were left in broth culture of 1.56 x 10(8) CFU/ml of S. mutans at 37 degreesC for 72 h for allowing bacteria to invade the cavity. The dentin bonding systems were applied separately to each of the two infected cavities, and the third cavity was left unapplied for control. After seating the occlusat surfaces, the teeth were kept in physiologic saline solution at 37 degreesC for 72 h. The standardized amounts of dentin chips (120 +/- 5 mg) were obtained from the cavity walls and the number of bacteria recovered was determined. The results were analyzed by One Way ANOVA, Kruskal Wallis and Mann-Whitney's U tests. Results. The primer of ABF and Reactmer Bond produced inhibition zones with similar sizes (p > 0.05), but the bonding resin of ABF did not produce any inhibition. When tested by the mode( cavity method, the application of ABF resulted in significantly less bacterial. recovery than Reactmer Bond (p < 0.05), demonstrating substantial antibacterial effects. Conclusions. The tooth model method used in this study was effective for evaluating the substantial, antibacterial effects of dentin bonding agents, and the experimental dentin bonding system ABF was demonstrated to be able to inactivate the bacteria in the cavity effectively in comparison with little antibacterial activity shown by Reactmer Bond.Öğe Effects of PCR-confirmed subclinical paratuberculosis on retinol and beta-carotene levels in dairy cattle(UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS, 2009) Civelek, T.; Celik, H. A.; Ozenc, E.; Avci, G.; Kav, K.; Cingi, C. C.; Yilmaz, O.In the present study, the effects of IS900 PCR-confirmed subclinical paratuberculosis on serum beta-carotene and retinol concentrations in dairy cattle were investigated. A total of 30 multiparous, clinically normal, paratuberculosis non-vaccinated Holstein dairy cows, ages 3 to 9 years old were tested. Fifteen of the 30 cows were diagnosed as having subclinical paratuberculosis by IS900 PCR on faecal samples (Group 1). The remaining 15 cows, negative to three independent tests for paratuberculosis, were classified as the healthy control group (Group 2). Serum retinol and beta-carotene levels were analyzed by colorimetric assays on serum samples from all 30 cows. There were no differences regarding the serum retinol and beta-carotene concentrations between cows with and without paratuberculosis.Öğe Identification of Enterobacteriaceae in Urfa cheese(AKADEMIAI KIADO, 2007) Ardic, M.; Kav, K.; Guner, A.; Dogruer, Y.The purpose of this study was to determine the Enterobacteriaceae counts and to identify the species isolated in Urfa cheese traditionally manufactured from raw milk in Sanliurfa. In the research, 75 samples obtained from retail markets were examined. Enterobacteriaceae was detected in 45.33% of the samples and Counts varied between 10(2)-10(8) CFU g(-1). A total of 8 different species were identified, the most common of those was Klebsiella pneumonia (30% of the isolated strains), followed by Escherichia coli (25.29%), Enterobacter cloacea (16.47%) and Enterobacler oerogenes (11.76%). Klebsiella oxytoca, Citrobacter freundii, Yersinia enterocolitica and Serratia mareescens were present in small quantities (7.06, 5.88, 2.45 and 1.18% of the isolates, respectively). The results obtained from this study clearly indicate that measures should be taken to control and reduce the contamination and multiplication of pathogens such as E. coli, Y enterocolitica and other Enterobacteriaceae spp. in Urfa cheese.
