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Öğe Acetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertility(WILEY, 2018) Kutchy, N. A.; Menezes, E. S. B.; Chiappetta, A.; Tan, W.; Wills, R. W.; Kaya, A.; Topper, E.Epigenetic modifications in histones are crucial for proper sperm physiology, egg activation and reproductive development of males. The objectives of this study were to determine the conservation and interactomes of histone three (H3) and ascertain the expression dynamics of acetylated and methylated H3 lysine 27 (H3K27ac and H3K27me3) in spermatozoa from Holstein bulls with different fertility. Methods in immunocytochemistry and flow cytometry were used to evaluate the expression dynamics of H3K27ac and H3K27me3 in spermatozoa from 10 bulls with different in vivo fertility. Computational biology methods including Clustal Omega and Cytoscape were performed to determine the evolutionary conservation and interactome of H3. The post-translational modifications (PTM) of H3 (H3K27ac and H3K27me3) had different spatiotemporal dynamics in the sperm head. Intensities of methylation were higher than those of acetylation and inversely correlated between the two fertility groups (p=.0032). The interacting proteins of H3 are involved in critical subcellular processes such as regulation of methylation, nucleosome assembly, regulation of DNA replication and chromatin assembly. These results are significant because they help advance fundamental science and biotechnology of mammalian reproduction.Öğe ANCA Positive Propylthiouracil Induced, Pyoderma Gangrenosum(Editrice Kurtis S R L, 2006) Gungor, K.; Gonen, S.; Kisakol, G.; Dikbas, O.; Kaya, A.Drugs from almost every class of pharmaceutic agent have been reported to cause vasculitis. Vasculitis among patients taking propylthiouracil (PTU) has been reported since 1950s (1) but relation of p-ANCA positive vasculitis in patients with hyper- thyroidism on PTU treatment was first reported by Stankus and Johnson in 1992 (2). In majority of the cases, approximately 80%, pyo- derma gangrenosum (PG) has been associated with systematic diseases such as ulcerative colitis, Crohn's disease, rheumatoid arthritis, Behcet's syndrome, monoclonal gammapathy (especially IgA) and chron- ic myeloid leukemia (3). There were also rare reports about drug induced PG reported to associate with iodide, bromide, isotretinoin, granulocyte colony stimulating factor and granulocyte-macrophage colony stimulating factor (4). PTU induced p-AN?A associated PG has been first reported by Darben et al. (5) in 1999. Hong et al. (6) reported second case in 2004. Here we report another case of PG in a p- ANCA positive patient treated for years with PTU for hyperthyroidism due to Graves' disease.Öğe Blood Pressure Control According to Cardiovascular Risk Factor Clustering: Tres 1 Study(Lippincott Williams & Wilkins, 2010) Erdine, S.; Arslan, E.; Şenocak, M.; Birler, A. G.; Cungurlu, A.; Karter, Y.; Erdem, A. M.; Kaya, A.[Abstract not Available]Öğe Diabetes and impaired glucose tolerance among Turkish immigrants in Sweden(ELSEVIER IRELAND LTD, 2011) Hjorleifsdottir-Steiner, K.; Satman, I.; Sundquist, J.; Kaya, A.; Wandell, P.Aim: To investigate whether the prevalence of diabetes and impaired glucose tolerance (IGT) was higher among Turkish immigrants in Sweden, than in their area of origin in Turkey. Methods: 238 Turkish immigrants aged 20 years and older living in Flemingsberg, Sweden, were compared with 1549 participants of the same age living in the Konya area of Turkey. Data collection included anthropometric measurements, blood pressure (BP) measurements, and an oral glucose tolerance test (OGTT). Results: Prevalence of laboratory-verified diabetes was 11.8% among participants in Sweden compared to 7.1% among participants in Turkey (p 0.018). Turkish women in Sweden had a higher prevalence of diabetes than Turkish women in Turkey, 12.8% vs. 7.6% (p = 0.037). Similarly, IGT was 17.8% among Turkish men in Sweden compared to 4.9% among men in Turkey (p < 0.001) and 2-h blood glucose was higher among the immigrants (p < 0.001). Systolic BP was also higher among the immigrants, especially in men (p < 0.001) who also had a higher BMI (p = 0.003). Conclusions: The higher prevalence of diabetes and IGT among Turkish immigrants in Flemingsberg, Sweden, suggests that migration is associated with diabetes and that there are important implications for public health in Sweden. (C) 2010 Elsevier Ireland Ltd. All rights reserved.Öğe DIABETES MELLITUS WITH LEFT TRANSVERSE AND SIGMOID SINUS THROMBOSIS EXTENDING INTO THE INTERNAL JUGULAR VEIN(EDITURA ACAD ROMANE, 2011) Guney, F.; Gumus, H.; Emlik, D.; Kaya, A.Background. Cerebral vein and sinus thrombosis (CVT) is less encountered, compared to arterial stroke. Commonly witnessed symptoms are headache, nausea, vomiting, confusion, aphasia, seizures, cranial nerve dysfunction and motor or sensorial deficits. The diagnosis is accurately determined by the help of MRI and MR venography. Multiple risk factors associated with CVT are present. Venous thrombosis tends to occur when there is an imbalance between prothrombotic and thrombolytic processes. Case report. In this report, a patient with CVT extending from left transverse and sigmoid sinuses to jugular vein and diagnosed with diabetes mellitus (DM) during this period was discussed in light of literature. The 55-year-old man was evaluated in the neurology clinic with the complaints of headache, nausea, vomiting and blurred speech. On neurologic examination, he was diagnosed with sensorial aphasia and consequently, with DM over the hospital stay. On the cranial MR venography, CVT thrombosis was detected, extending from transverse and sigmoid sinuses to internal jugular vein. Decreased level of protein C and shortage of aPTT were found. Anticoagulant treatment was carried out. All complaints were improved. Conclusion. In our subject, the existence of decreased protein C and shortage of APTT, along with DM, is a situation to increase hypercoagulability and the risk of cerebral vein and sinus thrombosis.Öğe The Effect of Misoprostol and Valethamate Bromide Administered Before Insemination With Frozen-Thawed Semen on Cervix Dilatation and Fertility in Sheep(Indian Veterinary Journal, 2000) Ataman, M. B.; Kaya, A.; Yıldız, C.; Düzgün, H.[Abstract not Available]Öğe The Effects of Aflatoxin on the Reproductive System of Roosters(W B Saunders Co Ltd, 2002) Ortatatlı, Mustafa; Çiftçi, M. K.; Tuzcu, Mehmet; Kaya, A.This study was conducted to determine the pathological changes in testes and epididymides and plasma testosterone levels of adult roosters during experimentally induced aflatoxicosis. In the study, 24 months of age, 32 Babcock breeder males were used, and they were divided into four groups each containing 8 animals. The groups were designed as follows; group 1: Control, no aflatoxin (AF), group 2: 5 ppm (parts per million) total aflatoxin (AF; B-1, B-2, G(1), G(2)), group 3: 10 ppm AF and group 4: 20 ppm AF in the diet, and the birds were fed for 8 weeks. Grossly, it was seen that the testes of all AF-treatment groups birds were significantly (P < 0,001) atrophied when compared with those of control birds. Histopathologically, there was no spermatogenesis in the testes of 4, 5 and 6 cocks fed on a diet containing AF 5, 10 and 20 ppm, respectively. Furthermore, abnormal spermatozoa were observed in some of AF-treatment groups (in 2 cases in each of 5 and 10 ppm AF-treated groups, and in one case in 20ppm AF-treated group). There were also mononuclear cell infiltration and/or focal lymphoid cell accumulation in the intertubular areas of the testes and epididymides in all AF-treatment groups, In conclusion, it has been shown that AF might totally or partially (dose related) suppress spermatogenesis, cause abnormality in spermatozoa and atrophy in testes. Furthermore, there was degeneration and desquamation in the epithelium and decrease in the size and thickness of the germinative layer of the seminiferous tubules, and lowered plasma testosterone levels in adult roosters.Öğe The Effects of Scrotal Insulation on Sperm Morphology and in Vitro Fertilization(Soc Study Reproduction, 2002) Enwall, L. E.; Ostermeier, G. C.; Bergfelt, R. S.; Laplante, L. N.; Kaya, A.; Parrish, J. J.[Abstract not Available]Öğe Impact of Physician Education on Blood Pressure Control in the Primary Care Setting: Results From Tres 1 Study(Lippincott Williams & Wilkins, 2010) Erdine, S.; Arslan, E.; Şenocak, M.; Birler, A. G.; Erdem, A. M.; Kaya, A.[Abstract not Available]Öğe The influence of homocysteine levels on endothelial function and their relation with microvascular complications in T2DM patients without macrovascular disease(SPRINGER-VERLAG ITALIA SRL, 2007) Karabag, T.; Kaya, A.; Temizhan, A.; Koc, F.; Yavuz, S.; Cam, S.The aim of this study was to investigate the influence of homocysteine (hcy) levels on endothelial function by the method of brachial artery ultrasonography and their relation with microvascular complications in type 2 diabetes mellitus (T2DM) patients without macrovascular disease. Fifty-nine T2DM patients with a mean age of 53.4 +/- 8.6 years and diabetes duration of 8.1 +/- 6.2 years and 16 healthy controls with a mean age of 47 +/- 14.5 years were included in the study. Endothelial-dependent and endothelium-independent flow-mediated dilatation (FMD) were evaluated via brachial artery ultrasonography. Fasting plasma glucose (FPG), glycosylated haemoglobin (A1c), lipid profile, hey, B12 and folic acid levels were measured. Diabetic patients and control group individuals were compared with regard to the laboratory values and brachial artery vascular reactivity. Factors influencing endothelium-dependent FMD were investigated with linear regression analysis. Age, gender, body mass index, lipid profiles and hey levels were similar in both groups (p > 0.05). Endothelium-dependent FMD percentages were significantly lower in diabetics than in the control group (7.7 +/- 5.9 vs. 11.7 +/- 7.1%, p < 0.05). Endothelial-independent FMD percentage was similar for both groups (p > 0.05). The upper limit of the reference hcy value was found to be 12.6 mu mol/l in the control group. In the diabetic group, hcy levels were high in 33 patients and normal in 26 patients. No difference was detected between the patients with high hcy levels and those with a normal level with regard to endothelium-dependent and endothelium-independent FMD values (p > 0.05). Mean hcy levels were 16 +/- 1.7 and 13.3 +/- 4.3 mu mol/l in T2DM patients with microvascular complication and those with no microvascular complication, respectively (p < 0.05). Regression analysis revealed that the main factors influencing the endothelial-dependent FMD were FPG, total cholesterol (TC), triglycerides (TG) and high-density lipoprotein (HDL-C) levels (p < 0.05, p=0.05, p=0.05, p=0.02, respectively). Hcy, folic acid and B 12 values did not influence endothelium-dependent FMD (p > 0.05). Diabetes duration and Ale levels were close to being significant although they did not reach statistical significance (p=0.07 and p=0.08 respectively). Hey levels have no effect on endothelium-dependent and endothelium-independent FMD in T2DM patients without macrovascular complications. The influence of classical atherogenic factors (such as FPG, TC, TG and HDL-C levels) on endothelium functions, detected with endothelium-dependent FMD, is greater.Öğe Influence of Melatonin Implantation on Sperm Quality, Biochemical Composition of the Seminal Plasma and Plasma Testosterone Levels in Rams(Ecole National Vet Toulouse, 2000) Kaya, A.; Başpınar, N.; Yıldız, C.; Kurtoğlu, F.; Ataman, M. B.; Haliloğlu, S.Influence of the melatonin implantation on sperm quality, plasma testosterone levels and biochemical composition of the seminal plasma was evaluated in rams in the breeding and non-breeding season. Melatonin administration during the non-breeding season improved progressive motility and morphologically normal sperm rates. The mean plasma testosterone level was significantly higher in melatonin treated animals compared to untreated ones. Fructose, sodium, calcium, magnesium levels, and aspartate aminotransferase and lactate dehydrogenase activity in the seminal plasma were similar in melatonin treated and control rams. In contrast, melatonin administration during the breeding season did not enhance spermatologic parameters and plasma testosterone levels. Biochemical composition of the seminal plasma was also comparable in melatonin treated and untreated control groups. In conclusion, melatonin administration during the breeding season did not influence sperm quality, plasma testosterone levels and biochemical parameters evaluated in this study.Öğe Methylation and Acetylation of Sperm Histone 3 Regulate Chromatin Dynamics and Male Fertility(AMER SOC CELL BIOLOGY, 2016) Chiappetta, A. M.; Kutchy, N. A.; Menezes, E.; Moura, A.; Kaya, A.; Perkins, A.; Memili, E.[Abstract not Available]Öğe Synchronization of Estrus in Cows Using Double Pgf(2 Alpha), Gnrh-pgf(2 Alpha) and Hcg-pgf(2 Alpha) Combination(Ecole Nationale Veterinaire Toulouse, 2003) Çoyan, K.; Ataman, Mehmet Bozkurt; Erdem, Hüseyin; Kaya, A.; Kasikci, GThe aim of this study was to compare the effectiveness of treatments combining GnRH and PGF(2alpha) hCG and PGF(2alpha) combinations, and double PGF(2alpha) administration for synchronization of estrus in cows. This study was carried out in 30 Brown Swiss cows, aging 3 - 5 years. The cows were randomly divided into three groups. In group I (n = 10), the cows were treated with an intramuscular injection of 20 mug GnRH (day = 0) at a random stage of the estrous cycle followed by intramuscular injection of 0.150 mg PGF(2alpha) 7 days later (day = 7). In group 11 (n = 10), the cows were treated with an intravenous injection of 3000 IU hCG (day = 0) at a random stage of the estrous cycle followed by intramuscular injection of 0.150 mg PGF(2alpha) 7 days later (day = 7). In group III (n = 10), the cows were received two injections of 0.150 mg PGF(2alpha) 11 day apart (day = 0, and 11). GnRH (10 mug) was injected intramuscularly to the cows 48 hours after the injection of PGF(2alpha) in the groups I and 11, and after the second PGF(2alpha) in group III. The cows were inseminated 12 hours after the GnRH injections. Blood samples were collected daily to determine plasma progesterone levels for 13 days in the groups I and H, and for 15 days in the group III starting from the day of GnRH, hCG and first PGF(2alpha) injections respectively. The mean interval injections-estrus, injections-ovulation, synchronization and pregnancy rates of the groups were determined. Plasma progesterone levels on day 0 and at estrous were similar among the three groups, but it was significantly higher (p < 0.01) in the groups I and 11 than in the group III on the day of PGF(2alpha) administration. The mean GnRH injections-estrus and injections-ovulation interval in the groups 1, 11 and III were 52.2 +/- 1.69, 48.4 +/- 1.34 and 68.2 +/- 1.31 hours; 70.4 +/- 1.17, 67.3 +/- 1.36 and 90.6 +/- 2.15 hours, respectively and were significantly different (p < 0.01). The synchronization rates tend to be higher in the groups I and 11 (100 %) than in the group 111 (80 %), and pregnancy rates of the groups 1, 11 and III were 60 %, 60 % and 30 %, respectively. As a conclusion, application of a GnRH and hCG analogue prior to synchronization of estrus with an injection of PGF(2alpha) may provide better results than two injections of PGF(2alpha) for estrus synchronization in cows.Öğe Uncovering sperm metabolome to discover biomarkers for bull fertility(BMC, 2019) Menezes, E. B.; Velho, A. L. C.; Santos, F.; Dinh, T.; Kaya, A.; Topper, E.; Moura, A. A.; Memili, E.Background Subfertility decreases the efficiency of the cattle industry because artificial insemination employs spermatozoa from a single bull to inseminate thousands of cows. Variation in bull fertility has been demonstrated even among those animals exhibiting normal sperm numbers, motility, and morphology. Despite advances in research, molecular and cellular mechanisms underlying the causes of low fertility in some bulls have not been fully elucidated. In this study, we investigated the metabolic profile of bull spermatozoa using non-targeted metabolomics. Statistical analysis and bioinformatic tools were employed to evaluate the metabolic profiles high and low fertility groups. Metabolic pathways associated with the sperm metabolome were also reported. Results A total of 22 distinct metabolites were detected in spermatozoa from bulls with high fertility (HF) or low fertility (LF) phenotype. The major metabolite classes of bovine sperm were organic acids/derivatives and fatty acids/conjugates. We demonstrated that the abundance ratios of five sperm metabolites were statistically different between HF and LF groups including gamma-aminobutyric acid (GABA), carbamate, benzoic acid, lactic acid, and palmitic acid. Metabolites with different abundances in HF and LF bulls had also VIP scores of greater than 1.5 and AUC- ROC curves of more than 80%. In addition, four metabolic pathways associated with differential metabolites namely alanine, aspartate and glutamate metabolism, beta-alanine metabolism, glycolysis or gluconeogenesis, and pyruvate metabolism were also explored. Conclusions This is the first study aimed at ascertaining the metabolome of spermatozoa from bulls with different fertility phenotype using gas chromatography-mass spectrometry. We identified five metabolites in the two groups of sires and such molecules can be used, in the future, as key indicators of bull fertility.
