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Öğe 8-Isoprostane and Coenzyme Q10 Levels in Patients with Metabolic Syndrome(MARY ANN LIEBERT, INC, 2016) Ercan, Humeyra; Kiyici, Aysel; Marakoglu, Kamile; Oncel, MufideBackground: Metabolic syndrome has become an important health problem, which involves obesity, hyper-lipidemia, insulin resistance, and high blood pressure values. The components of metabolic syndrome are all suggested as independent cardiovascular disease risk factors along with high mortality and morbidity rates accompanied by many organ and system complications. Objective: We aimed to determine 8-isoprostane (8-IsoP) and coenzyme Q10 (CoQ10) levels in patients with metabolic syndrome and healthy individuals and demonstrate whether there was any relation between these parameters and metabolic syndrome criteria. Materials and Methods: A total of 30 patients (10 male, 20 female) with metabolic syndrome and 20 age-matched healthy individuals (9 male, 11 female) were involved in the study. Body mass index, waist and hip circumferences, systolic and diastolic blood pressures and serum glucose, triglyceride, total cholesterol, high-density lipoprotein cholesterol, insulin, HbA1c, 8-IsoP and CoQ10 levels, and homeostasis model assessment of insulin resistance indexes of all participants were determined. Results: 8-IsoP levels were significantly increased in metabolic syndrome compared to healthy individuals (P = 0.003), however, there was no significant difference between groups for CoQ10 levels. 8-IsoP levels were positively correlated with waist circumference (r = 0.303, P=0.032), diastolic blood pressure (r = 0.337, P = 0.017), systolic blood pressure (r = 0.329, P = 0.020) values and total cholesterol levels (r = 0.354, P=0.012). Conclusion: We can suggest that the levels of 8-IsoP, which is an indicator of the oxidative stress, increase in metabolic syndrome and this can be associated with high blood pressure and visceral adiposity, which are the components of metabolic syndrome.Öğe Anti-inflammatory and anti-fibrotic effects of sirolimus on bleomycin-induced pulmonary fibrosis in rats(CANADIAN SOC CLINICAL INVESTIGATION, 2011) Tulek, Baykal; Kiyan, Esen; Toy, Hatice; Kiyici, Aysel; Narin, Cuneyt; Suerdem, MecitPurpose: Pulmonary fibrosis is a devastating disease with a poor prognosis. Although the diagnosis and pathophysiology of this disease have been better characterized over the past few years, there is no effective therapy for the disease. The aim of this study was to evaluate the anti-inflammatory and anti-fibrotic effects of sirolimus (SRL), which is a potential anti-fibrotic agent, by using bleomycin (BLM)-induced pulmonary fibrosis model in rats. Methods: A single intra-tracheal injection of BLM (2.5 U/kg) was administered and sirolimus (2.5 mg/kg/day) was given orally, beginning either one day before (early SRL) or nine days after (late SRL) the BLM administration. The effect of SRL on fibrosis was studied by analysis of cytokine levels in BAL fluid, measurement of lung tissue hydroxyproline (HPL) content and histopathological examination. Results: Both early and late SRL administrations caused a decrease in the levels of IL-13, PDGF-A and TGF-beta 1 (p=0.001) and an increase in IFN-beta levels (p=0.001) in BAL fluid. Early and late SRL also caused a decrease in HPL content (p=0.001). Early sirolimus caused a significant decrease in fibrosis score (p=0.001), while late SRL did not. Conclusion: Sirolimus was effective in BLM-induced pulmonary fibrosis model, especially in the early phases of the disease.Öğe Assessment of ischemia-modified albumin level in patients with recurrent pregnancy loss during the first trimester(ELSEVIER, 2011) Ozdemir, Suna; Kiyici, Aysel; Balci, Osman; Goktepe, Halime; Cicekler, Humeyra; Celik, CetinObjective: The finding that ischemia-modified albumin (IMA) is increased in pre-eclamptic pregnancy suggests a role for IMA as a potential biomarker for abnormal placental development related to miscarriage. This study was undertaken to evaluate IMA levels in women with recurrent pregnancy loss (RPL). Study design: This case-control study was performed between March 2008 and September 2009, at the Department of Obstetrics and Gynecology of Meram School of Medicine. Serum IMA and albumin concentrations were assessed in 43 women with a history of two or more unexplained first trimester miscarriages (group 1), and 42 healthy pregnant women (group 2) in the first trimester. IMA, adjusted IMA and albumin concentrations were compared between the groups. Statistical analysis was performed using Student's t-test and Mann-Whitney U test. Results: IMA and adjusted IMA levels were significantly higher in women with RPL (1.11 + 0.08 and 1.09 + 0.09, respectively) compared to women in group 2 (0.88 + 0.10 and 0.88 + 0.11, respectively). Albumin levels in group 1 were significantly lower compared with group 2. There was a negative correlation between IMAand albumin levels in each group. Conclusion: Maternal IMA levels appear to be elevated in women with early RPL This finding may suggest that an abnormally high hypoxic intrauterine environment may be associated with abnormal placental development that contributes to early miscarriage. (C) 2010 Elsevier Ireland Ltd. All rights reserved.Öğe The Association Among Lipoprotein-associated Phospholipase A2 Levels, Total Antioxidant Capacity and Arousal in Male Patients with OSA(IVYSPRING INT PUBL, 2011) Bekci, Taha T.; Kayrak, Mehmet; Kiyici, Aysel; Maden, Emin; Ari, Hatem; Kaya, Zeynettin; Teke, TurgutBackground: The mechanisms of the increased cardiac and vascular events in patients with OSA are not well understood. Arousal which is an important component of OSA was associated with increased sympathetic activation and electrocardiographic changes which prone to arrhythmias. We planned to examine the association among arousal, circulating Lp-PLA2 and total antioxidant capacity in male patients with OSA. Methods: Fifty male patients with newly diagnosed OSA were enrolled the study. A full-night polysomnography was performed and arousal index was obtained. Lp-PLA2 concentrations were measured in serum samples with the PLAC Test. Total antioxidant capacity in patients was determined with Antioxidant Assay Kit. Results: Arousal was positively correlated with LP-PLA2 levels (r=0.43, p=0.002) and was negatively correlated with total antioxidant capacity (r= -0.29, p=0.04). Elevated LP-PLA2 levels and decreased total antioxidant activities were found in the highest arousal quartile compared with the lowest and 2nd quartiles (p=0.02, p=0.05, respectively). LP-PLA2 was an independently predictor of arousal index in regression model (beta=0.357, p=0.002) Conclusions: This study demonstrated a moderate linear relationship between arousal and LP-PLA2 levels. Also, total antioxidant capacities were decreased in the higher arousal index. Based on the study result, the patients with higher arousal index may be prone to vascular events.Öğe Association of paraoxonase-1 activity and major depressive disorder in patients with metabolic syndrome(SPRINGER INDIA, 2015) Ari, Hatem; Kayrak, Mehmet; Gunduz, Mehmet; Kayhan, Fatih; Kaya, Zeynettin; Kiyici, Aysel; Uguz, FarukAssociations between metabolic syndrome (MS) and major depressive disorder (MDD) are well documented although the underlying biological mechanisms for this relationship are less studied. Paraoxonase (PON1) is a high-density lipoprotein (HDL)-associated enzyme, with demonstrated evidence of strong antioxidant activity. Oxidative stress has been implicated in the pathophysiology of MS and MDD. PON1 activity has been studied to some extent in patients with MS and less in MDD. The aim of this study was to compare serum PON1 activity in patients with MS and MDD, MS without MDD, and normal control groups in the context of the biological mechanism of the association between MS and MDD. In this case-control study, 67 patients with MS and 25 healthy controls from the hospital-university staff were recruited. All patients and healthy controls were assessed by a semi-structured psychiatric interview. Patients with MDD were diagnosed according to the DSM-IV criteria for MDD. Serum PON1 activity was determined with a spectrophotometric method, and the activity was compared between patients with MS and MDD, with MS but without MDD, and control groups. Serum PON1 activity levels were lower in patients with MS and MDD group compared to those in the patients with MS and without MDD group and control group (69.5+/-24.2, 84.3+/-34.6, and 97.1+/-40.8 U/ml, p=0.03, respectively). Post hoc analysis showed that PON1 activity was statically significantly lower in the MS with MDD group than in the control group (p=0.02). Impaired PON1 activity, in the context of enhanced oxidative stress, could be one of the possible underlying biological mechanisms of the MS-MDD association.Öğe Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis(WALTER DE GRUYTER GMBH, 2013) Vatansev, Husamettin; Ozturk, Bahadir; Yilmaz, Sema; Sivrikaya, Abdullah; Dagli, Mehmet; Kiyici, Aysel; Unlu, AliObjective: Rheumatoid arthritis (RA) is a chronic inflammatory disease and it is associated with premature atherosclerosis development. RA patients have multiple factors to develop premature atherosclerosis. Enhanced asymmetric dimethylarginine (ADMA) is considered as a risk factor for the development of atherosclerosis by inducing endothelial dysfunction. The aim of this study was to investigate the role of asymmetric dimethylarginine, arginine and citrulline levels in patients with RA and their relation to the disease activity parameters for possible role on the disease activity. Methods: 92 patients with RA and 34 healthy controls were included the study. Patients and control blood samples collected for ADMA, arginine and citrulline levels. ADMA, arginine and citrulline levels were measured by pre-column derivatization fluorescence HPLC method. CRP and ESR levels examined on the same day were taken from the patient records. Results: While ADMA levels significantly increased, arginine levels decreased in the patient group. There was no difference in citrulline levels between both groups. Only citrulline and arginine showed a weak positive correlation. ADMA, arginine, and citrulline levels showed no correlation with either biochemical parameters such as CRP or ESR, or disease activity or disease severity. Conclusion: Our study has shown that an increase in ADMA levels with the decrease in arginine levels suggests an increase in arginine turnover to ADMA. Increased ADMA levels can be used for an increased risk for premature development of atherosclerosis in patients with RA. Since high ADMA levels prevent the NO-dependent vasodilatation, a possible role of ADMA in development of premature atherosclerosis may be independent from inflammation.Öğe Chemical Composition of Nigella sativa L. Seeds Used as a Medical Aromatic Plant from East Anatolia Region, Turkey(ASIAN JOURNAL OF CHEMISTRY, 2013) Vatansev, Husamettin; Ciftci, Harun; Ozkaya, Ahmet; Ozturk, Bahadir; Evliyaoglu, Nurcan; Kiyici, AyselIn this study, chemical composition such as vitamins, fatty acids and trace elements of Nigella sativa L. seeds collected from East Anatolia Region of Turkey were investigated. Fatty acids in the lipid extracts were converted into methyl esters. The methyl esters were extracted with n-hexane, they were separated and quantified by gas chromatography. Fat soluble vitamins were determined by HPLC and the mixture of acetonitrile/methanol (3/1, v/v) was used as the mobile phase. For determination of trace elements levels, solutions from microwave digestion of a certain amount of seeds were analyzed with inductively coupled plasma optical emission spectrometry. Main fatty acids of Nigella sativa L. seeds were found as 66.5 and 23.5 (as relative % peak area) for linoleic acid (18: 2) and oleic acid (18: 1), respectively. The contents of Co, Ni, Fe, Zn, Cu, Mn and Cr were determined as 0.12, 1.48, 117.32, 41.42, 30.26, 28.56 and 2.55 mu g/g (dry matter) respectively. The levels of vitamins were found as 10.19 mu g/g for alpha-tocopherol, 2.28 mu g/g for delta-tocopherol, 0.18 mu g/g for retinol, 1.38 mu g/g for vitamin D-2 1.85 mu g/g for vitamin K-1 and 2.15 mu g/g for vitamin K-2. The Nigella sativa L. seeds were found to be rich in unsaturated fatty acids, vitamins and trace elements, suggesting that they may be valuable for apoptosis and would be appropriate to further studies in this direction.Öğe Clinical and metabolic effects of medroxyprogesterone acetate and ethinyl estradiol plus drospirenone in women with polycystic ovary syndrome(WILEY, 2008) Ozdemir, Suna; Gorkemli, Huseyin; Gezginc, Kazim; Ozdemir, Mustafa; Kiyici, AyselObjectives: To investigate the effects of treatment with medroxyprogesterone acetate (MPA), 10 days per month for 6 months, on lipid and carbohydrate metabolism in women with polycystic ovary syndrome (PCOS). Methods: Sixty-three women with PCOS were randomized to receive MPA or ethinyl estradiol plus drospirenone. Results: There were no changes in lipid or carbohydrate metabolism in the MPA group, but serum levels of luteinizing hormone (P < 0.001) and total testosterone (P < 0.003) significantly decreased, as did the free androgen index (P < 0.02) and acne (P < 0.03) and seborrhea (P < 0.04) scores. In the ethinyl estradiol plus drospirenone group lipid and hormone values significantly increased whereas acne, seborrhea, hair Loss, and Ferriman-Gallwey scores decreased. There was no statistically significant change in the total cholesterol to high-density cholesterol ratio in either group. Conclusion: Treatment of PCOS patients with MPA provided good menstrual cycle control, beneficial changes in hormonal values associated with hyperandrogenism, and no significant changes in lipid or carbohydrate metabolism. (c) 2008 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All, rights reserved.Öğe Compared effects of surgical and natural menopause on climacteric symptoms, osteoporosis, and metabolic syndrome(WILEY, 2009) Ozdemir, Suna; Celik, Cetin; Gorkemli, Huseyin; Kiyici, Aysel; Kaya, BugraObjective: To compare the effects of surgical (ie, earlier) and natural (ie, later) menopause oil climacteric symptoms, osteoporosis. and metabolic syndrome. Method: The study was conducted with 94 women who underwent hysterectomy and bilateral oophorectomy and 95 women who were older than 40 years and in natural menopause. None had received hormone theraphy or osteoporosis treatment. Metabolic syndrome was defined according to the National Cholesterol Education Program Adult Treatment Panel III. Results: The rates of hot flushes (P=0.001), sweating (P=0.001), poor memory (P=0.04), change in sexual desire (P=0.04), and osteoprosis (diagnosed in the hip bone, P=0.005) were significantly higher among the women in Surgical menopause, but the rate of metabolic syndrome was similar in the 2 groups (47.8% and 40%; P=0.28). Conclusion: Compared with natural menopause, surgical menopause was found to be associated with highter rates of climacteric symptoms and osteoporosis but not of of metabolic syndrome. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. on behalf of International Federation of Gynecology and Obstetrics. All rights reserved.Öğe A comparison of the effects of aggressive dose and conventional dose atorvastatin applications on IL-6 and NO levels in patients with acute myocardial infarction(ACADEMIC JOURNALS, 2011) Basarali, Mustafa Kemal; Buyukbas, Sadik; Yazar, Hayrullah; Kiyici, Aysel; Kayrak, Mehmet; Ulgen, SiddikHigh dose statin medication in acute coronary syndrome cases is a therapy which lowers mortality and morbidity rates. Interleukin-6 (IL-6) is produced in higher amounts in acute myocardial infarction (MI) and facilitates myocardial damage. However, secretion of nitric oxide (NO) is depleted. We aimed to compare the effects of conventional dose (10-40 mg/day) and aggressive dose (80 mg/day) atorvastatin medications on IL-6 and NO levels in patients with primary percutaneous transluminal coronary angioplasty (PTCA) intervention after acute MI. 50 patients (8 females, 42 males) with the diagnosis of acute MI with ST segment elevation enrolled to the study. Primary PTCA intervention was performed on these patients and consequently either conventional dose (10 to 40 mg/day) or aggressive dose (80 mg/day) atorvastatin medications were given to the patients. Three months later, plasma IL-6 and NO levels were determined and alterations in the groups were evaluated. IL-6 levels decreased from 24.34 +/- 12.04 to 11.40 +/- 5.79 pg/ml and from 29.62 +/- 17.38 to 12.51 +/- 8.95 pg/ml in conventional dose and aggressive dose regimens respectively (p<0.001). However, NO concentrations increased from 22.90 +/- 8.24 to 31.70 +/- 7.56 mu M in conventional dose and from 19.37 +/- 5.60 mu M to 34.15 +/- 9.60 mu M in aggressive dose groups (p < 0.001). The effects of aggressive dose atorvastatin medication on IL-6 and NO levels were similar to conventional dose application in cases with ST segment elevation acute MI.Öğe Cytokeratin 18 and h-FABP levels in intestinal ischemia-reperfusion injury: role of coenzyme Q10(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2013) Belviranli, Muaz; Okudan, Nilsel; Gokbel, Hakki; Kiyici, Aysel; Oz, Mehmet; Kumak, AyseAim: The objective of this study was to investigate whether coenzyme Q10 (CoQ10) supplementation affects M30 and heart-type fatty acid binding protein (h-FABP) levels in intestinal ischemia reperfusion injury-induced rats. Materials and methods: Thirty-two male Wistar rats were randomly divided into 4 groups. 1) Sham: Animals exposed to laparotomy without clamping the superior mesenteric artery (SMA); 2) ischemia-reperfusion (IR): rats exposed to laparotomy with occlusion of the SMA for 45 mm, followed by 120 min of reperfusion period; 3) CoQ10 plus sham: 10 mg kg body weight(-1) CoQ10 was administrated via intraperitoneal injection for 20 days, and thereafter animals were exposed to laparotomy without clamping the SMA; 4) CoQ10 plus IR: 10 mg kg body weight(-1) CoQ10 was administrated via intraperitoneal injection for 20 days and thereafter animals were exposed to laparotomy with occlusion of the SMA for 45 mm, followed by 120 min of reperfusion period. Blood samples were taken by cardiac puncture for the analysis of serum h-FABP and M30 levels. Results: There was no difference among the groups for serum h-FABP and M30 antigen levels. Conclusion: Neither intestinal ischemia-reperfusion injury nor CoQ10 supplementation affect serum h-FABP and M30 levels in rats.Öğe Cytokines, adipocytokines and inflammatory markers in patients on continuous ambulatory peritoneal dialysis and hemodialysis(TAYLOR & FRANCIS LTD, 2016) Oncel, Mufide; Akbulut, Seval; Ozer, Turkan Toka; Kiyici, Aysel; Keles, Mustafa; Baltaci, Beyhan; Turk, SuleymanBackground: Cytokines are essential mediators of immune response. Chronic renal failure patients suffer from chronic inflammation that results from factors such as impaired renal function, accumulation of uremic toxins and bio incompatibility of dialyzer membranes. These patients are also at increased risk of cardiovascular diseases. We have evaluated cytokines, adipocytokines and inflammatory markers in patients with chronic renal failure undergoing hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD).Material and methods: We have determined serum tumor necrosis factor- (TNF-), interleukin-6 (IL-6), high-sensitivity C-reactive protein (hsCRP), leptin and ghrelin levels of chronic renal failure patients treated with either HD (n=20) or CAPD (n=20). TNF-, IL-6, ghrelin and leptin measurements were performed by commercially available kits based on enzyme-linked immunosorbent assay (ELISA) method. hsCRP levels were determined by turbidimetric methods.Results: Serum TNF- and IL-6 levels of patients on HD were significantly higher than those of the ones on CAPD (p<0.05). Ghrelin, leptin and hsCRP concentrations were similar in both groups.Conclusions: We can conclude that cytokine production is more obvious in HD process.Öğe Does obesity change the effect of aromatase inhibitors (AIs) on estradiol, leptin, insulin, and IGF-1 serum levels in breast cancer patients?(AMER SOC CLINICAL ONCOLOGY, 2012) Artac, Mehmet; Askin, Dudu; Kiyici, Aysel; Eren, Onder Orhan; Oncel, Mufide; Boruban, Melih Cem; Bozcuk, Hakan Sat[Abstract not Available]Öğe THE EFFECT OF ILOPROST ON RENAL DYSFUNCTION AFTER RENAL I/R USING CYSTATIN C AND beta(2)-MICROGLOBULIN MONITORING(LIPPINCOTT WILLIAMS & WILKINS, 2009) Sahsivar, M. Orkun; Narin, Cueneyt; Kiyici, Aysel; Toy, Hatice; Ege, Erdal; Sarigul, AliThe purpose of this study was to investigate the effect of iloprost, a cytoprotective prostacyclin analog, on renal injury during unilateral renal I/R in rats and to determine whether the levels of serum cystatin C (CyC) and beta(2)-microglobulin (B2M), as markers of glomerular function, might denote this injury. Thirty-two Wistar rats were randomized into four groups (n = 8) as follows: control (sham laparotomy), renal I/R (60-min left renal ischemia and 120-min reperfusion), renal I/R + iloprost (20 ng kg(-1) min(-1) infusion during renal I/R period, i.v.), and control + iloprost. Blood and kidney tissue samples were obtained for biochemical and histological analysis from all rats. Serum urea, creatinine, CyC, and B2M levels were evaluated for biochemical analysis. Histopathological changes in renal structure were examined for histological analysis. Serum urea, creatinine, and CyC levels were significantly increased in the renal I/R group. Iloprost treatment decreased these three markers in the renal I/R + iloprost group. beta(2)-Microglobulin levels were not significantly changed in any group. Histological analyses showed that renal I/R elicited significant renal injury, whereas iloprost significantly decreased I/R-induced renal injury. Serum CyC level is one of the good indicators of acute renal damage due to I/R produced by renal artery occlusion. In contrast, we have shown that there are no significant changes in the levels of serum B2M levels that would make it an accurate diagnostic tool for detecting acute changes in renal injury subject to renal I/R in rats.Öğe The Effects of Alpha Lipoic Acid on Cerebral Vasospasm Following Experimental Subarachnoid Hemorrhage in the Rabbit(TURKISH NEUROSURGICAL SOC, 2011) Erdi, Mehmet Fatih; Guney, Onder; Kiyici, Aysel; Esen, HasanAIM: The aim of this study is to determine the effects of a strong dithiol antioxidant, alpha lipoic acid (ALA) on cerebral vasospasm following subarachnoid hemorrhage in a rabbit model. MATERIAL and METHODS: Twenty-one New Zealand white rabbits were assigned to one of three groups: group 1 (control), group 2 (SAH only), group 3 (SAH+ALA). ALA was administered (100 mg/kg/day, single dose, intraperitoneally). The rabbits were sacrificed 72 hours after SAH. The basilar artery lumen areas, arterial wall thickness and endothelial apoptosis in a cross section of basilar artery were measured in all groups. The tissue MDA, SOD, GSH-Px levels were also determined. RESULTS: The elevated tissue MDA levels after SAH were significantly reduced by ALA treatment. The reduced tissue SOD and GSH-Px levels after SAH were also elevated by ALA treatment. In the treatment group the average wall thickness and the mean percentages of apoptotic cells (apoptotic index) were reduced and the average cross-sectional areas of the basilar artery were increased statistically significantly. CONCLUSION: ALA treatment attenuates the severity of cerebral vasospasm by its strong antioxidant, antivasospastic and antiapoptotic properties. ALA may potentially serve as agents in the prevention of cerebral vasospasm after SAH.Öğe The effects of melatonin on oxidative stress markers in an animal model of radiocontrast-induced nephropathy(ALLIED ACAD, 2011) Bayir, Aysegul; Kara, Hasan; Kiyici, Aysel; Kiyici, Halil; Ak, AhmetWe investigated the effects of melatonin on erythrocyte and renal tissue superoxide dismutase (SOD) and malondialdehyde (MDA) levels in rabbits after the administration of a radiocontrast agent. Twenty-four New Zealand rabbits were divided into four groups, six rabbits in each: control, sham, hydration, and hydration plus melatonin. Rabbits in the control group were sacrificed after the extraction of renal tissue. All other rabbits received a single dose of IV diatrizoat sodium (10 mL/kg). In the hydration group, saline (10 mL/kg IV) was infused at 6 hour intervals. In the hydration plus melatonin group, melatonin (10 mg/kg IV) and saline (10 ml/kg IV) were administered at 6 hour intervals. Venous blood samples were obtained from the rabbits before, and 48 and 72 hours after diatrizoat sodium administration to measure serum urea, creatinine, sodium, potassium, calcium, and erythrocyte MDA levels and SOD activities. Renal tissues were removed at the end of 72 hours, and tissue MDA levels and SOD activities were determined. At 72 hours, erythrocyte MDA concentrations of the hydration plus melatonin animals were significantly lower than those of the sham and hydration groups. However, erythrocyte and renal tissue SOD activities were significantly higher in the hydration plus melatonin group than the other groups. Renal tissue MDA levels of the hydration plus melatonin group were significantly lower than those of the sham and hydration groups. Melatonin has favorable effects on lipid peroxidation and antioxidant status in this animal model of radiocontrast nephropathy.Öğe Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats(SOC BIOLGIA CHILE, 2012) Tulek, Baykal; Kiyan, Esen; Kiyici, Aysel; Toy, Hatice; Bariskaner, Hulagu; Suerdem, MecitStatins reduce cholesterol levels by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase and have a major place in the treatment of atherosclerotic disease. Recent studies have shown anti-inflammatory properties of statins. The purpose of this study was to evaluate the anti-inflammatory effect of simvastatin on bleomycin (BLM)-induced pulmonary fibrosis in rats. A total of 31 female Sprague-Dawley rats were divided into four groups: (1) intratracheal (IT) phosphate-buffered saline (PBS) + intraperitoneal (IP) PBS (n=7); (2) IT BLM + IP PBS (n=8); (3) IT BLM + low dose (LD) simvastatin (1 mg/kg daily, n=8); (4) IT BLM + high dose (HD) simvastatin (5 mg/kg daily, n=8). Simvastatin was administered IP for 15 days, beginning 1 day prior to IT BLM. The effect of simvastatin on pulmonary fibrosis was studied by measurements of IL-13, PDGF, IFN-gamma, TGF-beta 1 levels in bronchoalveolar lavage (BAL) fluid and lung tissue hydroxyproline (HPL) content and by histopathological examination (Ashcroft score). BLM caused significant change in BAL fluid cytokine levels and increased both HPL content and histopathological score (p<0.001 for all). While LD simvastatin had no effect on cytokine levels, HD significantly reduced IL-13 (15.12 +/- 7.08 pg /ml vs. 4.43 +/- 2.34 pg/mL; p<0.05) and TGF-beta 1 levels (269.25 +/- 65.42 pg/mL vs. 131.75 +/- 32.65 pg/mL; p<0.05). Neither HD nor LD simvastatin attenuated HPL content or Ashcroft score. In conclusion, this study showed that LD simvastatin had no effect on a BLM-induced pulmonary fibrosis model, while the high dose caused partial improvement in profibrotic cytolcine levels.Öğe THE EVALUATION OF THE EFFECTS OF GRAPE SEED EXTRACT ON SERUM PARAOXONASE ACTIVITY IN STREPTOZOTOCIN-INDUCED DIABETIC RATS(SPRINGER TOKYO, 2009) Gokbel, Hakki; Kiyici, Aysel; Okudan, Nilsel; Belviranli, Muaz[Abstract not Available]Öğe Evaluation of the levels of oxidative stress factors and ischemia modified albumin in the cord blood of smoker and non-smoker pregnant women(TAYLOR & FRANCIS LTD, 2012) Sahinli, Ayla Songul; Marakoglu, Kamile; Kiyici, AyselObjective: The aim of this cross-sectional study was to determine the oxidant and antioxidant status and a novel ischemia marker - ischemia modified albumin - in the cord blood of smoker and non-smoker pregnants. Methods: This study was performed on 30 smoker and 60 non-smoker pregnant women. Malondialdehyde (MDA), vitamin A and E, total antioxidant capacity (TAC) and ischemia modified albumin (IMA) levels and superoxide dismutase activities (SOD) were determined in the cord blood of the contributors. Results: In the cord blood of the smoker women compared to the non-smokers; MDA (mu mol/L) levels increased (5.17 +/- 0.25, 3.60 +/- 0.06, p = 0.000), IMA (ABSU) levels increased (0.913 +/- 0.02, 0.830 +/- 0.01, p = 0.050), SOD (U/ml) activities decreased (8.22 +/- 0.14, 8.63 +/- 0.14, p = 0.045), Vit A (mu g/L) (339.06 +/- 17.52, 454.91 +/- 16.56, p = 0.000) and Vit E (mg/L) levels decreased (2.8 +/- 0.15, 7.58 +/- 0.38, p = 0.000) and TAC (Mm Trolox) levels decreased (3.25 +/- 0.15, 4.08 +/- 0.09, p = 0.000), and these differences were statistically significant. We found moderate and strong positive correlations between smoking status and IMA (r = 0.325, p = 0.002) and smoking status and MDA levels (r = 0.636, p = 0.000). Smoking status presented weak, moderate and strong negative correlations with SOD activities, TAC, Vit A and Vit E levels, respectively (r = -0.237, p = 0.024), (r = -0.420, p = 0.000), (r = -0.443, p=0.000), (r = -0.795, p = 0.000). Conclusion: It was determined that smoking cigarette during gestation disturbed the balance between the oxidant and antioxidant system and caused oxidative stress. Increased IMA levels in cord blood of smoker pregnants suggests that smoking during pregnancy causes fetal ischemia.Öğe Homocysteine levels and total antioxidant capacity in children with acute rheumatic fever(PERGAMON-ELSEVIER SCIENCE LTD, 2008) Kurban, Sevil; Mehmetoglu, Idris; Oran, Bulent; Kiyici, AyselObjective: The aim of the study was to investigate total antioxidant capacity (TAC) and homocysteine levels in children with acute rheumatic fever (ARF). Design and methods: Nineteen patients with ARE and twenty healthy children, age- and sex-matched were included in the study. Follow-up studies were made at the 7(th), 14(th), 21(st) and 28(th) day of diagnosis. Results: Children with ARF had significantly higher serum homocysteine levels and lower TAC than the same parameters of the controls at all measurements. Following the anti-inflammatory therapy, we found a progressive increase in TAC and a decrease in homocysteine levels of the patients. Conclusion: We concluded that increased serum homocysteine levels and decreased serum TAC of the patients with ARF can be considered as a sign of increased inflammation and oxidative stress in these patients which needs to be considered during therapy. Further studies are needed to understand the underlying mechanisms of these findings. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
