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    New insights into the in vitro biological effects, in silico docking and chemical profile of clary sage - Salvia sclarea L.
    (ELSEVIER SCI LTD, 2018) Zengin, Gökhan; Şenkardeş, İsmail; Mollica, Adriano; Picot-Allain, Carene Marie Nancy; Bulut, Gizem; Doğan, Ahmet; Mahomoodally, M. Fawzi
    Salvia sclarea L. is traditionally used to manage common human ailments and is consumed as a food product. This study aimed to establish the phytochemical profile and antioxidant potential of ethyl acetate, methanol, and water extracts of Salvia sclarea. The inhibitory action of the extracts against alpha-amylase, alpha-glucosidase, acetyicholinesterase, butyrylcholinesterase, and tyrosinase was also investigated. Methanol extract showed the highest phenolic and fiavonoid contents (81.78 mg GAE/g extract and 40.59 mg RE/g extract, respectively). Reversed phase high performance liquid chromatography with diode array detector analysis revealed that S. sclarea was rich in rosmarinic acid. The water extract exhibited the lowest inhibitory activity against alpha-amylase but the upmost activity against alpha-glucosidase (0.19 and 18.24 mmol ACAE/g extract, respectively). Experimental data showed that only the water extract (8.86 mg KAE/g extract) significantly inhibited tyrosinase. Docking studies showed that quercetin binds to tyrosinase by two hydrogen and a pi-pi bonds. Salvia sclarea showed interesting biological activity against key enzymes involved in the pathogenesis of common ailments. (C) 2018 Elsevier Ltd. All rights reserved.
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    Sea rose (Armeria pungens (Link) Hoffmanns. & Link) as a potential source of innovative industrial products for anti-ageing applications
    (ELSEVIER SCIENCE BV, 2018) Rodrigues, Maria J.; Pereira, Catarina A.; Oliveira, Marta; Neng, Nuno R.; Nogueira, Jose M. F.; Zengin, Gökhan; Mahomoodally, M. Fawzi
    This work explored for the first time the halophyte Armeria pungens (Link) Hoffmann. & Link (sea rose) as a potential source of industrial natural products with anti-ageing potential. In this sense, ethyl acetate, acetone, ethanol and water extracts were prepared from flowers, peduncles and leaves of sea rose and evaluated for in vitro antioxidant capacity by four complementary methods and enzyme inhibitory effects towards enzymes related with the onset of different diseases, namely acetyl-(AChE) and butyrylcholinesterase (BuChE), alpha-glucosidase, tyrosinase, lipase and elastase. The polyphenolic composition of the extracts was appraised by highpressure liquid chromatography coupled with diode array detection analysis (HPLC-DAD). Nineteen phenolic compounds were identified in the extracts, including flavonoids, hydroxybenzoic and hydroxycinnamic acids, and catechin was the main compound detected. The ethanol extract of sea rose leaves was the most effective as DPPH (IC50 = 83.7 mu g/mL) and ABTS (IC50 = 146 mu g/mL) scavenger, and also as a copper chelator (IC50 = 81.8 mu g/mL). The acetone peduncles' extract had the highest iron chelating activity (IC50 = 142 mu g/mL). Water and ethanol leaf extracts showed the uppermost AChE inhibition (water: IC50 = 87.6 mu g/mL; ethanol: IC50 = 90.3 mu g/mL). In turn, the ethanol flower extract had the lowest IC50 values towards a-glucosidase (26.7 mu g/mL) and tyrosinase (268 mu g/mL). The leaf ethanol extract had the highest capacity to inhibit elastase (IC50 = 531 mu g/mL), while none of the extracts had significant inhibitory activity on BuChE and lipase. In conclusion, ethanol extracts from leaves and flowers of sea rose showed to be the most promising source of natural products for industrial applications, with anti-ageing properties, namely neuroprotective, anti-diabetic, anti-wrinkles and anti-melanogenic activities.