Yazar "Nogueira, Jose M. F." seçeneğine göre listele

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    In vitro enzyme inhibitory and anti-oxidant properties, cytotoxicity and chemical composition of the halophyte Malcolmia littorea (L.) R.Br. (Brassicaceae)
    (TAYLOR & FRANCIS LTD, 2020) Castaneda-Loaiza, Viana; Placines, Chloe; Rodrigues, Maria Joao; Pereira, Catarina G.; Zengin, Gökhan; Neng, Nuno R.; Nogueira, Jose M. F.
    This work reports for the first time the in vitro anti-oxidant (towards DPPH, ABTS, copper and iron), enzymatic inhibitory (on AChE, BuChE, alpha-glucosidase, alpha-amylase and tyrosinase), cytotoxicity (towards HepG2 and HEK 293 cells), and metabolomics (by HPLC-MS) of extracts from organs of Malcolmia littorea (L.) R.Br. Extracts were constituted mainly by phenolic acids and flavonoids, and main compounds were salicylic acid and luteolin-7-O-glucoside. Samples showed reduced radical scavenging and metal chelating capacity, and only the methanol extracts reduced iron. The root's ethanol and methanol extracts, and the aerial organ's ethanol extract exhibited the highest AChE inhibition. The root's ethanol extract displayed dual anti-cholinesterase activity. Samples showed a low capacity to inhibit alpha-amylase, but a high alpha-glucosidase inhibition was obtained with the root's and flower's ethanol extracts, and flower's methanol extract. Overall, samples displayed a high inhibition against tyrosinase, reduced HepG2 cellular viability and were less toxic towards HEK 293 cells.
  • Küçük Resim Yok
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    Sea rose (Armeria pungens (Link) Hoffmanns. & Link) as a potential source of innovative industrial products for anti-ageing applications
    (ELSEVIER SCIENCE BV, 2018) Rodrigues, Maria J.; Pereira, Catarina A.; Oliveira, Marta; Neng, Nuno R.; Nogueira, Jose M. F.; Zengin, Gökhan; Mahomoodally, M. Fawzi
    This work explored for the first time the halophyte Armeria pungens (Link) Hoffmann. & Link (sea rose) as a potential source of industrial natural products with anti-ageing potential. In this sense, ethyl acetate, acetone, ethanol and water extracts were prepared from flowers, peduncles and leaves of sea rose and evaluated for in vitro antioxidant capacity by four complementary methods and enzyme inhibitory effects towards enzymes related with the onset of different diseases, namely acetyl-(AChE) and butyrylcholinesterase (BuChE), alpha-glucosidase, tyrosinase, lipase and elastase. The polyphenolic composition of the extracts was appraised by highpressure liquid chromatography coupled with diode array detection analysis (HPLC-DAD). Nineteen phenolic compounds were identified in the extracts, including flavonoids, hydroxybenzoic and hydroxycinnamic acids, and catechin was the main compound detected. The ethanol extract of sea rose leaves was the most effective as DPPH (IC50 = 83.7 mu g/mL) and ABTS (IC50 = 146 mu g/mL) scavenger, and also as a copper chelator (IC50 = 81.8 mu g/mL). The acetone peduncles' extract had the highest iron chelating activity (IC50 = 142 mu g/mL). Water and ethanol leaf extracts showed the uppermost AChE inhibition (water: IC50 = 87.6 mu g/mL; ethanol: IC50 = 90.3 mu g/mL). In turn, the ethanol flower extract had the lowest IC50 values towards a-glucosidase (26.7 mu g/mL) and tyrosinase (268 mu g/mL). The leaf ethanol extract had the highest capacity to inhibit elastase (IC50 = 531 mu g/mL), while none of the extracts had significant inhibitory activity on BuChE and lipase. In conclusion, ethanol extracts from leaves and flowers of sea rose showed to be the most promising source of natural products for industrial applications, with anti-ageing properties, namely neuroprotective, anti-diabetic, anti-wrinkles and anti-melanogenic activities.