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Öğe COMPARATIVE HISTOMETRICAL STUDY OF THE CEREBELLUM AND THE DETERMINATION OF SOME AgNOR PARAMETERS IN DIFFERENT AVIAN SPECIES(NATL VETERINARY RESEARCH INST, 2011) Sur, Emrah; Oznurlu, Yasemin; Ozaydin, Tugba; Colakoglu, Fatma; Unsal, Sadettin; Yener, YesimThe study was carried out to determine some characteristics of silver stained nucleolus organising regions (AgNOR parameters) of the Purkinje cells and structural differences of the cortex, if any, in the cerebellum of turkeys, ducks, pigeons, and starlings. The thickness of the molecular and granular layer at the summit of the folia was the highest in pigeons, whereas the highest value of the molecular and granular layer was determined at the deep of the folia in turkeys. The highest number of Purkinje cells per unit scale was observed in pigeons and starlings. The mean sizes of the Purkinje cells were greater in turkeys than in other species. The mean area of the Purkinje cell nucleus, AgNOR area and AgNOR counts were found to be higher in turkeys. However, there was no difference in the mean ratio of AgNOR area to Purkinje cell nucleous area among the species. In conclusion, it seems that the results obtained from this study could be of particular interest to comparative biologists and physiologists.Öğe Comparative Skin Histology of the White New Zealand and Angora Rabbits: Histometrical and Immunohistochemical Evaluations(MEDWELL ONLINE, 2009) Oznurlu, Yasemin; Celik, Ilhami; Sur, Emrah; Telatar, Tugba; Ozparlak, HalukIn this study, thickness of the skin sub layers and the number, absolute and relative areas of the nuclear Argyrophilic Nucleolar Organiser Regions (AgNORs) and the number of Proliferating Cell Nuclear Antigen (PCNA) positive nuclei of the germinal matrix epithelial cells of the Angora and white New Zealand rabbits were compared. Skin samples were taken from 40 adult healthy rabbits. General histological features of the skin in both Angora and white New Zealand rabbits were quite similar and displayed the characteristics of thin skin. Angora rabbit skin was significantly (p<0.05) thicker than that of the white New Zealand rabbit and the difference mainly originated from the thicker papillary layer of the dermis. The Angora rabbit had the greatest hair follicle number in the unit area of the dermis. The shape, dimension and distribution pattern of the AgNORs were similar in both rabbit strains. The number, absolute and relative areas of the AgNORs and PCNA immunoreactivity of the Angora rabbits were higher than those of the White New Zealand rabbits. The histological characteristics of the skin in the white New Zealand rabbit were suitable for leather industry, whereas the Angora rabbit had good hair follicle characteristics for wool production. Further studies on a larger population regarding seasonal differences should be done to elucidate the possible association between AgNOR parameters and PCNA immunoreactivity of germinal matrix cells of hair follicles and wool production.Öğe Determination of the activity of alpha naphthyl acetate esterase and acid phosphatase of peripheral blood lymphocytes in healty pregnant merino sheep(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2013) Sur, Emrah; Aydin, Ibrahim; Oznurlu, Yasemin; Ozaydin, Tugba; Celik, Ilhami; Kadiralieva, NaristeThis study was performed to determine the effects of pregnancy on the activities of alpha naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes in pregnant Merino sheep. Peripheral blood lymphocyte percentages were also estimated. Periferal blood samples were taken from animals in six different gestational stages as non-pregnant control, in the first, the second, the third, the fourth and the fifth month of pregnancy. Each group was contained 20 animals. The lowest ANAE (+) lymphocytes percentage (63.5%) was determined in the first month of pregnancy whereas the highest null lymphocytes proportion (12.75%) was detected in the same gestational period. There were statistically decreases in the proportions of peripheral blood lymphocyte (42.9%) and the ACP-ase (+) lymphocytes (43.35%) in the last gestational stages. Although the posibble hormonal changes may cause the fluctuation of peripheral blood lymphocyte proportions in all gestational periods, the most distinctive changes were observed at the begining and at the end of the pregnancy. It was concluded that the data was obtained from this study was useful for understanding of the possible mechanisms of maternal tolerance.Öğe The distribution of pas-positive uterine natural killer (unk) cells in the decidua basalis of pregnant mice(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2015) Sur, Emrah; Oznurlu, Yasemin; Ozaydin, Tugba; Celik, Ilhami; Aydin, Ibrahim; Kadyralieva, NaristeThis study was carried out to determine the distribution of uterine natural killer (uNK) cells having periodic-acid Schiff (PAS) positive granules in the decidua basalis in pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as nonpregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n=6 for each group). After placentation, it was identified four different regions as metrial region (MLAp; mesometrial lymphoid aggregate of pregnancy), decidua basalis, junctional zone and labyrinth zone in mice. It was observed trophoblast giant cells between decidua basalis and junctional zone. The number of PAS-positive uNK cells was found as 5 in 10.000 mu m(2) in endometrium of control animals while it was determined as 26.5 number/10.000 mu m(2) in decidua basalis at early gestational period. The highest PAS-positive uNK cell numbers (56 number/10.000 mu m(2)) were detected at the mid-gestational period in decidua basalis. uNK cells were also observed in the lumen of the maternal blood vessels. In mesometrial region, the dilatation of maternal blood vessels and thinning of the media layer was distinct. It was concluded that the number and the distribution of the uNK cells having periodic-acid Schiff (PAS) positive granules was affected by pregnancy.Öğe Effects of aflatoxin on the proportions of peripheral blood leukocytes and alpha-naphtyl acetate esterase (anae) positive lymphocytes in the mouse(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2010) Tuzcu, Mehmet; Sur, Emrah; Celik, Ilhami; Oznurlu, Yasemin; Ciftci, Mustafa KemalIn this study detrimental effects of aflatoxin (AF) on the peripheral blood formula and the alpha naphthyl acetate esterase (ANAE) - positivity profile of peripheral blood lymphocytes (PBL) on the mice were determined. A total of 40 white mice were randomly divided in to 5 groups each having 8 animals. The first group served as a control and received standard diet. The remaining 4 groups were fed diets containing 200, 400, 800 and 1600 ppb AF, respectively. The results of this study have revealed that AF given by the food caused significant declines both in the proportion of PBL and in the percentages of ANAE-positive peripheral blood lymphocytes (T-lymphocytes) in a dose dependent manner. These results may be concerned as a haematological evidence for immuno-suppressive effect of AF on cell-mediated immunity.Öğe Histological and Histochemical Evaluations on the Effects of High Incubation Temperature on the Embryonic Development of Tibial Growth Plate in Broiler Chickens(WILEY, 2016) Oznurlu, Yasemin; Sur, Emrah; Ozaydin, Tugba; Celik, Ilhami; Uluisik, DenizThe effects of experimentally induced high incubation temperature on the embryonic development of growth plate of the chicken were investigated by means of histological and enzyme histochemical methods. In the experiments, 250 fertile eggs of Ross-308 broiler strain were divided into two groups, the control eggs were maintained under optimal conditions (37.8 degrees C and 65% +/- 62% relative humidity, rh) during the whole incubation period. Heat-stress imposed eggs were maintained under normal conditions (37.88C and 65% 62% rh) until the 10th day of incubation, and then, continuously (24 h per day) exposed to high temperature (38.8 degrees C and 65% +/- 62% rh). Tissue samples were taken from 10 animals of each group at the 11th, 13th, 15th, 18th, 21st days of incubation. Tissue samples were processed by enzyme histochemical methods in addition to routine histological techniques. The relative tibia weights and tibia length were lower in the heat-stress group compared to the control group. The results of the measurements of the growth plate showed that the proliferative zone was narrowed whereas, the transitional and hypertrophic zone were thickened in the heat stress group. Alkaline phosphatase (ALP) density was significantly decreased in the heat-stress group compared to the control group. In conclusion, bone formation and growth plate formation are crucial for embryo development and 1 degrees C higher from optimum may increase the incidence of skeletal disorders and leg problems in broiler chickens which is one of the major animal welfare concerns for the poultry industry. Microsc. Res. Tech. 79: 106-110, 2016. (C) 2016 Wiley Periodicals, Inc.Öğe Histological evaluation of the effects of bioglass, hydroxyapatite, or demineralized freeze-dried bone, grafted alone or as composites, on the healing of tibial defects in rabbits(SAUDI MED J, 2009) Kucukkolbasi, Hasan; Mutlu, Necip; Isik, Kubilay; Celik, Ilhami; Oznurlu, YaseminObjectives: To compare the effectiveness of bioactive glass (BG), natural hydroxyapatite (HA), and demineralized freeze-dried bone (DFDB) in bone defects. Methods: All animal experiments were conducted in Faculty (if Veterinary Medicine, Selcuk University, Konya, Turkey, under the Selcuk University Guidelines for Animal Experimentation, in 2005. Eighteen New Zealand rabbits were used for the experiment. Four cavities were prepared on right and left tibias. The cavities on the right tibia were filled with either BG, HA or DFDB. One cavity was left empty as a control. The cavities on the other tibia] bone were grafted with HA+BG, DFA+DFDB, BG+DFDB and HA+BG+DFDB composites. Histological examinations were performed at first, third, and sixth postoperative months. Results: According to histomorphometric findings, the mixture containing HA+BG+DFDB obtained the best histological results (P<0.05). Conclusion: The composite graft of HA, BG and DFDB is more effective than when used as individual agents.Öğe Histological examination of the skin and AgNOR parameters of matrix pili cells in the chinchilla(2011) Oznurlu, Yasemin; Çelik, İlhami; Sur, Emrah; Özaydın, TuğbaÇinçilyalarda derinin histolojisi ve kıl matriks pili hücrelerinde AgNOR sayısının ışık mikroskobik seviyede incelenmesi amaçlandı. Gereç ve Yöntem: Onbeş adet ergin ve sağlıklı çinçilyadan alınan deri örnekleri tamponlu formol salin solüsyonunda tespit edildi ve rutin histolojik yöntemlerle parafinde bloklandı. Bloklardan alınan kesitler Crossmon’un üçlü boyaması, Gordon ve Sweet’in retiküler iplik boyaması, Verhoef’in elastik iplik boyaması ve AgNOR boyama yöntemleriyle boyandı. Derinin toplam kalınlığı ile epidermis ve dermis kalınlıkları, birim alandaki (1 mm2) ortalama kıl folikül sayıları ve germinal matriks pili hücrelerinde AgNOR sayısı ve alanı belirlendi. Bulgular: Germinal matriks hücrelerinin çekirdeklerindeki AgNOR’ların kahverengi-siyah lekeler halinde 1 ile 4 adet arasında değişen sayılarda olduğu tespit edildi. Çinçilyalarda birleşik kıl foliküllerinin bir ya da iki primer folikül çevresinde çok sayıda sekonder folikülden oluştuğu gözlendi. Sekonder foliküllerin primer foliküllere oranı (S/P) 13/1 olarak tespit edildi. Öneri: Daha geniş populasyonlarda ve mevsimsel değişiklikler göz önüne alınarak yapılacak çalışmalarda, kıl foliküllerinin sayı ve dağılımı ile germinal matriks hücrelerindeki AgNOR parametrelerinin belirlenmesi seleksiyonda kürk kalitesi için önemli bir kriter olabilir.Öğe Immunohistochemical Distribution of Heat Shock Protein 70 and Proliferating Cell Nuclear Antigen in Mouse Placenta at Different Gestational Stages(WILEY, 2016) Ozaydin, Tugba; Sur, Emrah; Oznurlu, Yasemin; Celik, Ilhami; Uluisik, DenizThe aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation. (C) 2016 Wiley Periodicals, Inc.Öğe The proportion and the distribution of t-lymphocytes, null lymphocytes and acid phosphatase positive lymphocytes of the peripheral blood and endometrium in pregnant mice(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2014) Sur, Emrah; Oznurlu, Yasemin; Ozaydin, Tugba; Celik, Ilhami; Aydin, Ibrahim; Kadiralieva, NaristeThis study was performed to determine the effects of pregnancy on the proportion of T-lymphocytes, null lymphocytes and acid phosphatase (ACP-ase) positive peripheral blood lymphocytes and the distribution of the mentioned cells in the decidua basalis region of endometrium in the pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as non-pregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n = 6 for each group). The lowest T-lymphocytes percentage was determined at early and middle pregnancy in the peripheral blood (43.83%) and decidua basalis (10.83 number/0.1 mm(2)), respectively. There was a statistically significant increase in the proportions of the peripheral blood ACP-ase (+) lymphocytes (44.33%) at the mid-gestational period while the lowest ACP-ase positive lymphocyte numbers (5.50 number/0.1 mm(2)) in the decidua basalis was observed at early pregnancy. The highest null cell rates were found at early gestation either in the peripheral blood (11.50%) or in the decidua basalis (7.83 umber/0.1 mm(2)). The lowest percentage of peripheral blood lymphocyte (56.00%) was recorded at the early pregnancy. It was concluded that the number and the distribution of the lymphocyte, T-lymphocyte, null lymphocyte, and ACP-ase positive lymphocyte were affected by pregnancy although there were some differences among the gestational periods.Öğe Use of yeast cell walls and Yucca schidigera extract in layer hens' diets(PAGEPRESS PUBL, 2011) Gurbuz, Emel; Balevi, Tahir; Kurtoglu, Varol; Oznurlu, YaseminThis research was conducted to determine the impact of diet supplementation with yeast cell walls (YCW) and Yucca schidigera extract (YE) on performance, egg weight, specific gravity, body weight, and intestinal tissue histology in layer hens. White, 48-week-old, Hyline hybrid hens (n= 320) were divided into four main groups, each comprising eight groups of 10 hens: (1) control, (2) 500 mg/kg YCW added, (3) 500 mg/kg YE added and (4) 250 mg/kg YE plus 2500 mg/kg YCW added. While the egg production and feed intake of the hens was significantly affected, overall feed efficiency, damaged-egg ratio, dirty-egg ratio, egg weight and specific gravity did not differ between the control group and the YCW, YE or YCW+YE groups. Final body weight was higher in the YCW, YE and YCW+YE groups than in the control group. There were differences in the width, muscle layer thickness and height/crypt depth ratio of the duodenal villus and the width of the ileal villus among the four groups. It can be concluded that YCW and YCW+YE supplementation for layer hens are beneficial for egg production.
