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Öğe Assessment of In vitro Antibacterial Activity and Cytotoxicity Effect of Nigella sativa Oil(MEDKNOW PUBLICATIONS & MEDIA PVT LTD, 2016) Ugur, Ayse Ruveyda; Dagi, Hatice Turk; Ozturk, Bahadir; Tekin, Gulsum; Findik, DuyguBackground: Methicillin resistance is a serious health concern since it has spread among Staphylococcus aureus and coagulase-negative Staphylococci (CoNS) that are frequent community and nosocomial pathogens worldwide. Methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. Nigella sativa oil is known to be active against Gram-positive cocci, yet its in vitro cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of methicillin-resistant isolates. Objectives: The aim of this study is to evaluate the in vitro antibacterial activity and cytotoxicity effect of N. sativa oil. Materials and Methods: The minimal inhibitory concentrations (MICs) of N. sativa oil were determined by broth microdilution method against four different American Type Culture Collection strains, 45 clinical isolates of methicillin-resistant S. aureus (MRSA), and 77 methicillin-resistant CoNS (MRCoNS). The effects of different dilutions (0.25 mu g/mL, 0.5 mu g/mL, and 1 mu g/mL) of N. sativa oil on the proliferation of gingival fibroblasts were evaluated. Results: The MIC values of N. sativa oil against clinical isolates of Staphylococci were between < 0.25 mu g/mL and 1.0 mu g/mL. Compared to the control group, there was no cytotoxic effect on the proliferation of the gingival fibroblasts. Conclusion: In the present study, the oil of N. sativa was very active against MRSA and MRCoNS and had no in vitro cytotoxicity at relevant concentrations. These findings emphasize that there is a requirement for further clinical trials on N. sativa oil for "safe" medical management of infections caused by methicillin-resistant Staphylococci.Öğe Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis(WALTER DE GRUYTER GMBH, 2013) Vatansev, Husamettin; Ozturk, Bahadir; Yilmaz, Sema; Sivrikaya, Abdullah; Dagli, Mehmet; Kiyici, Aysel; Unlu, AliObjective: Rheumatoid arthritis (RA) is a chronic inflammatory disease and it is associated with premature atherosclerosis development. RA patients have multiple factors to develop premature atherosclerosis. Enhanced asymmetric dimethylarginine (ADMA) is considered as a risk factor for the development of atherosclerosis by inducing endothelial dysfunction. The aim of this study was to investigate the role of asymmetric dimethylarginine, arginine and citrulline levels in patients with RA and their relation to the disease activity parameters for possible role on the disease activity. Methods: 92 patients with RA and 34 healthy controls were included the study. Patients and control blood samples collected for ADMA, arginine and citrulline levels. ADMA, arginine and citrulline levels were measured by pre-column derivatization fluorescence HPLC method. CRP and ESR levels examined on the same day were taken from the patient records. Results: While ADMA levels significantly increased, arginine levels decreased in the patient group. There was no difference in citrulline levels between both groups. Only citrulline and arginine showed a weak positive correlation. ADMA, arginine, and citrulline levels showed no correlation with either biochemical parameters such as CRP or ESR, or disease activity or disease severity. Conclusion: Our study has shown that an increase in ADMA levels with the decrease in arginine levels suggests an increase in arginine turnover to ADMA. Increased ADMA levels can be used for an increased risk for premature development of atherosclerosis in patients with RA. Since high ADMA levels prevent the NO-dependent vasodilatation, a possible role of ADMA in development of premature atherosclerosis may be independent from inflammation.Öğe Asymmetric dimethylarginine levels in Behcet's disease(WALTER DE GRUYTER GMBH, 2014) Ozturk, Bahadir; Vatansev, Husamettin; Taner, Alpaslan; Yilmaz, Sema; Dagli, Mehmet; Unlu, AliObjective: Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging.Öğe Chemical Composition of Nigella sativa L. Seeds Used as a Medical Aromatic Plant from East Anatolia Region, Turkey(ASIAN JOURNAL OF CHEMISTRY, 2013) Vatansev, Husamettin; Ciftci, Harun; Ozkaya, Ahmet; Ozturk, Bahadir; Evliyaoglu, Nurcan; Kiyici, AyselIn this study, chemical composition such as vitamins, fatty acids and trace elements of Nigella sativa L. seeds collected from East Anatolia Region of Turkey were investigated. Fatty acids in the lipid extracts were converted into methyl esters. The methyl esters were extracted with n-hexane, they were separated and quantified by gas chromatography. Fat soluble vitamins were determined by HPLC and the mixture of acetonitrile/methanol (3/1, v/v) was used as the mobile phase. For determination of trace elements levels, solutions from microwave digestion of a certain amount of seeds were analyzed with inductively coupled plasma optical emission spectrometry. Main fatty acids of Nigella sativa L. seeds were found as 66.5 and 23.5 (as relative % peak area) for linoleic acid (18: 2) and oleic acid (18: 1), respectively. The contents of Co, Ni, Fe, Zn, Cu, Mn and Cr were determined as 0.12, 1.48, 117.32, 41.42, 30.26, 28.56 and 2.55 mu g/g (dry matter) respectively. The levels of vitamins were found as 10.19 mu g/g for alpha-tocopherol, 2.28 mu g/g for delta-tocopherol, 0.18 mu g/g for retinol, 1.38 mu g/g for vitamin D-2 1.85 mu g/g for vitamin K-1 and 2.15 mu g/g for vitamin K-2. The Nigella sativa L. seeds were found to be rich in unsaturated fatty acids, vitamins and trace elements, suggesting that they may be valuable for apoptosis and would be appropriate to further studies in this direction.Öğe DETERMINATION OF SERA NGAL LEVELS IN PATIENTS WITH LUNG CANCER AND ITS RELATION WITH APOPTOSIS(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2018) Ozturk, Bahadir; Ciftci, Harun; Vatansev, Husamettin; Gun, Fatma Gul; Sunam, Guven Sami; Oncel, Murat; Karabagli, PinarThe NGAL synthesis induces in neoplasms; therefore, probably, it is benefited from the level of this protein for the determination of carcinogenesis and the progress stages of human tumors. In this study, it was aimed to investigate the expression of NGAL (neutrophil gelatinase associated lipocalin) in lung cancer patients and its relation with apoptosis. The study was conducted on the patients between the ages of 40 and 70 years accepted by the Thoracic Surgery Clinic (Selcuk University, Faculty of Medicine) based on their lung cancer diagnosis. The patients aged between 40 and 70 were included in the study from the thoracic surgery clinic of the Selcuk University as they were administered into the clinic based on their lung cancer diagnosis and had operation before. Patients who have acute stroke, rheumatic diseases, chronic kidney disease, congestive heart failure, chronic infection and other organ and system cancers (except lung cancer) were excluded from the study. Two groups including a lung cancer group (N=40) and a normal group (N=40) were formed to determine the NGAL and M30 levels in sera of patients using the ELISA method. According to the analysis results, the NGAL levels for the lung cancer group and the normal group were observed as 424.03 +/- 74.49 and 374.04 +/- 90.34 ng/mL, respectively. This increase in the lung cancer group was found statistically significant according to the normal group (p < 0.01). The M30 levels, marker for apoptosis in circulation, were obtained as 144.08 +/- 45.91 and 118.76 +/- 46.16 U/L for the lung cancer group and the normal group, respectively. This increase in the lung cancer group was found statistically significant according to the normal group (p < 0.01). A positive correlation was obtained between the NGAL and M30 data by means of Spearman's correlation test (r(40)=0.58, p < 0.01). The antigen levels of caspase 3, 8 and 9 were determined by the immunohistochemical staining methods in the samples of cancerous tissues and normal tissues adjacent to the cancerous tissue, and the apoptotic indexes were calculated. The apoptotic indexes of the cancerous tissues were significantly lower than the normal tissues (p < 0.01). This result demonstrates that the predominant type of cell death might be other cell death pathways rather than the apoptotic pathways in lung cancer. In addition, these results support the opinion about the fact that apoptosis may be inhibited by cancer cells in cancerous tissues. The significant increase in the M30 and NGAL levels supports the claims of the previous studies about the fact that it causes the consumption of cellular iron reserves and NGAL induced apoptosis in cancer patients.Öğe Effect of pinealectomy on homocysteine levels in ovariectomized rats(MAGHIRA & MAAS PUBLICATIONS, 2007) Oztekin, Esma; Ozturk, Bahadir; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Tiftik, Ali MuhtarOBJECTIVE: Hyperhomocysteinemia plays a role in obstetric and gynecological diseases and such diseases are seen as a consequence of an impaired methionine cycle. In the present study, it was aimed to determine the effect of estradiol and progesterone on homocysteine levels in pinealectomized and ovariectomized rats. MATERIALS AND METHODS: The study was carried out on 24 adult Spraque-Dawley female rats. Rats were divided into 4 groups as follows: Group 1: Sham ovariectomy group (Sham-Ovx), Group 2: Ovariectomized and sham pinealectomized group (Ovx-Sham-Px), Group 3: Ovariectomized and pinealectomized group (Ovx-Px), Group 4: Group which were supplemented with estradiol and progesterone after ovariectomy and pinealectomy (Ovx-Px-E+P). Serum homocysteine levels were determined after experimental period. RESULTS: Homocysteine levels in Group 3 were higher than those in Groups 1, 2 and 4 (p<0.05). Homocysteine levels in Groups 2 and 4 were higher than those in Group 1 (p<0.05). Homocysteine levels in Groups 2 and 4 were not different. CONCLUSION: The findings of the present study demonstrate that ovariectomy lead to increases homocysteine levels. Pinealectomy in addition to ovariectomy increases homocysteine levels. However, administration of estradiol and progesterone following pinealectomy prevents the increase in homocysteine levels in ovariectomized rats.Öğe Effect of Static Magnetic Field on Oxidant/Antioxidant Parameters in Cancerous and Noncancerous Human Gastric Tissues(HINDAWI LTD, 2016) Ozturk, Bahadir; Durak, Zahide Esra; Buber, Suleyman; Kocaoglu, Ender HilmiAim. To investigate the effects of static magnetic field (SMF) on oxidant and antioxidant parameters of the cancerous and noncancerous human gastric tissues. Materials and Methods. Gastric tissues obtained from patients with gastric cancer were used in the study. SMF was created by using two static magnets. Before and after treatment with SMF, oxidant and antioxidant parameters were measured in the tissue samples. Results. In the cancerous tissue, superoxide dismutase (SOD) activity was found higher and malondialdehyde (MDA) level was found lower as compared with noncancerous tissue. SMF affects oxidant/antioxidant parameters differently in the cancerous and noncancerous tissues. In this regard, SMF causes increase in SOD activity and decrease in MDA level in the noncancerous tissue. However, it decreases SOD and glutathione peroxidase (GSH-Px) activities and increases MDA level and catalase (CAT) activity in the cancerous tissue. There were no differences between nitric oxide (NO) and nitric oxide synthase (NOS) parameters in or among the cancerous and noncancerous tissues. Conclusions. SMF accelerates peroxidation reactions possibly by suppressing SOD and GSH-Px enzymes in the cancerous gastric tissue. This event caused by SMF might play part in the death of cancer cells, which may be a good supportive vehicle for the cancer therapy.Öğe THE EFFECT OF THYMOQUINONE ON THE miRNA PROFILE OF MCF-7 BREAST CANCER CELLS(INT JOURNAL PHARMACEUTICAL SCIENCES & RESEARCH, 2017) Saracligil, Beyza; Ozturk, Bahadir; Bozkurt, S. Buket; Kahveci, YaseminBackground and aim: Thymoquinone (TQ), which is the most bioactive component of Nigella sativa (Black cumin), exhibits anticancer characteristics based on cell culture and experimental animal studies. However, molecular action mechanisms of these effects are not clear. MicroRNAs (miRNAs), small non-coding RNAs of approximately 22 nucleotides, are an emerging class of gene expression modulators with relevant roles in several biological processes, including cell differentiation, development, apoptosis, and regulation of the cell cycle. The purpose of this study was to investigate the potential impact of thymoquinone (TQ) on MCF-7 human breast cancer cell miRNAs. Materials and methods: The expression levels of miRNAs in MCF-7 cell and TQ treated MCF-7 cells were estimated by miRNA sequencing. The expressions of miRNAs were determined real-time qPCR. Results: We detected 10 down-regulated mirRNAs (hsa-miR-1, let 7c-5p, hsa-miR-15b-5p, hsa-mir-202-3p, hsa-miR-214-3p, hsa-miR-210-3p, hsa-miR-31-5p, hsa-miR-424-5p, hsa-miR-497-5p, hsa-miR-98-5p) and 2 up regulated miRNAs (hsa-miR-22-3p, hsa-miR-132-3p) in TQ treated groups, comparing with control group. These findings highlight the effects of TQ miRNA profile and molecular mechanism on MCF-7 cells. Conclusion: Finally, according to computational analyses using validated databases PI3 kinase/AKT (hsa04151), Wnt (hsa04310), MAPK (hsa04010) and p53 (hsa 04115) signaling pathways seem to be the key targets of these TQ groups of miRNA.Öğe Effects of aqueous extract from Silybum marianum on adenosine deaminase activity in cancerous and noncancerous human gastric and colon tissues(MEDKNOW PUBLICATIONS & MEDIA PVT LTD, 2015) Ozturk, Bahadir; Kocaoglu, Ender Hilmi; Durak, Zahide EsraObjective: Investigation of possible effects of Silybum marianum extract (SME) on adenosine deaminase (ADA) activity in cancerous and noncancerous human gastric and colon tissues to obtain information about possible mechanism of anticancer action of S. marianum. Materials and Methods: Cancerous and noncancerous human gastric and colon tissues removed from patients by surgical operations were used in the studies. The extract was prepared in distilled water. Before and after treatment with the extract, ADA activities in the samples were measured. Results: ADA activity was found to be lowered significantly in cancerous gastric tissues but not in noncancerous gastric tissues after treatment with the SME. In the colon tissues, ADA activities were however found to increase after the treatment of SME. Conclusion: Our results suggest that the aqueous extract from S. marianum inhibits ADA activity in cancerous gastric tissues significantly. It is suggested that in addition to other proposed mechanisms, accumulated adenosine due to the inhibition of ADA might also play a part in the anticancer properties of the S. marianum.Öğe Effects of epilepsy and antiepileptic drugs on nitric oxide, lipid peroxidation and xanthine oxidase system in children with idiopathic epilepsy(W B SAUNDERS CO LTD, 2011) Arhan, Ebru; Serdaroglu, Ayse; Ozturk, Bahadir; Ozturk, H. Serdar; Ozcelik, Aysima; Kurt, Nese; Kutsal, EbruThe aim of this study is to investigate the effects of epilepsy, valproic acid and oxcarbazepine on nitric oxide levels, lipid peroxidation and xanthine oxidase levels in newly diagnosed epileptic children and healthy controls. A total of 49 patients with newly diagnosed idiopathic epilepsy and 15 healthy children were enrolled in this study. Of these 49 patients, 16 children were treated with valproate and 16 treated with oxcarbazepine. Nitric oxide, malondialdehyde and xanthine oxidase levels prior to antiepileptic drug therapy were measured in the serum. Blood samples were drawn before antiepileptic drug therapy and after 3 and 6 months of the antiepileptic drug treatment. Nitric oxide levels were statistically higher in the newly diagnosed epileptic patients. In oxcarbazepine group, the nitric oxide and malondialdehyde levels were found to be decreased. No statistically significant differences were noted in nitric oxide, malondialdehyde and xanthine oxidase levels in valproic acid treated group. Oxcarbazepine which is a frequently used new antiepileptic drug in childhood epilepsy may modify nitric oxide levels and lipid peroxidation. These results suggest that decreased lipid peroxidation would play a role in the mechanism of antiepileptic effects by oxcarbazepine treatment. (C) 2010 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.Öğe The effects of exogenous estradiol and progesterone with melatonin on homocysteine levels in ovariectomized rats(INDIAN VETERINARY JOURNAL, 2007) Oztekin, Esma; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Ozturk, Bahadir; Tiftik, Ali MuhtarHomocysteine, a product of methionine cycle, plays a role in obstetric and gynaecological diseases and these diseases are considered as a result of impaired methionine cycle (Obsweger et al., 1994). Estrogen is considered a nongenetic factor influencing homocysteine metabolism (Pines et al., 1997) Homocysteine levels can be a risk factor for arteriosclerosis and osteoporosis (El-Swefy et al., 2002). El-Swefy et al., (loc. cit) found that high homocysteine levels in ovariectomized rats decreased with estradiol and folic acid supplementation. The aim of this study was to investigate effect of replacement of estradiol, progesterone and melatonin on homocysteine levels in ovariectomized rats.Öğe The effects of melatonin and colchicine on ischemia-reperfusion injury in experimental rat testicular torsion model(W B SAUNDERS CO-ELSEVIER INC, 2017) Sekmenli, Tamer; Gunduz, Metin; Ozturk, Bahadir; Karabagli, Pinar; Ciftci, Ilhan; Tekin, Gulsum; Yilmaz, MustafaPurpose: The aim of the present study is to investigate the efficiency of colchicine and melatonin in an experimental rat testicular torsion model in the light of histological and biochemical data. Methods: A total of 34 Wistar albino male rats were randomly divided into 5 groups as: Group C (control, n= 6), Group S (sham; underwent only left scrotal exploration, n= 7), Group TD (torsion and detorsion; 6 h of ischemia and 7 days of reperfusion, n= 7), Group TD/M (TD + Melatonin; 6 h of ischemia and 7 days of reperfusion and 7 days of 17 mg/kg intraperitoneal melatonin per day, n = 7), group TD/Col (TD + Colchicine; 6 h of ischemia and 7 days of reperfusion and 7 days of 1 mg/kg oral colchicine per day, n = 7). Histopathologic evaluation of seminiferous tubule deterioration was performed by Johnsen's scoring system. Total antioxidant status (TAS), total oxidant status (TOS), IL-6, TNF alpha levels were analyzed in each group. Results: The histopathologic scores, total antioxidant status (TAS), total oxidant status (TOS), IL-6, TNF alpha levels in groups C and TD/Col were significantly lower than groups TD and TD/M (P < .001). Conclusion: Our study results revealed that colchicine reduced testicular ischemia-reperfusion injury in experimental rat testis torsion model. Although detorsion of testis is crucial for the preserving the testicular viability, antioxidant and anti-inflammatory treatment modalities like colchicine might help to reduce ischemia-reperfusion injury in detorsed testis. (C) 2017 Elsevier Inc. All rights reserved.Öğe In vivo analysis of urinary stones with dual-energy computed tomography(LIPPINCOTT WILLIAMS & WILKINS, 2019) Erdogan, Hasan; Temizoz, Osman; Koplay, Mustafa; Ozturk, BahadirObjective Formation mechanisms and treatment of the urinary stones are different, depending on their chemical structure. Therefore, determining the stone type plays a key role in planning treatment and preventive measures. Computed tomography (CT), with the use of dual-energy technology in recent years, has made it possible to do in vivo analysis of urinary stones. In this study, we aimed to evaluate the diagnostic efficacy of dual-energy CT (DECT) and compare its results with in vitro analysis, which is accepted as a gold standard for analysis of urinary stones. Materials and Methods The DECT examinations were performed on 373 patients using 128-slice dual-source CT scanner. Analysis of attenuation ratios in the high and low kilovoltage peak values of the stone was performed at workstation, and stones were classified as hydroxyapatite, calcium oxalate, cystine, and uric acid. On follow-up, the stone was obtained in 35 patients as a result of surgery or passed spontaneously. The DECT analysis and in vitro analysis results were compared and statistically evaluated. Results In all patients, 136 hydroxyapatite, 160 calcium oxalate, 57 uric acid, and 20 cystine stones were detected with DECT. In vitro analyses of the stones were performed in 35 patients, and 8 hydroxyapatite, 18 calcium oxalate, 6 uric acid, and 3 cystine stones were revealed. When DECT analysis results were compared with in vitro analysis results, stone types were detected correctly in 32 (91.4%) patients and incorrectly in 3 (8.6%) patients. Especially all uric acid and cystine stones were correctly detected with DECT. Conclusions With advanced postprocess analysis methods, DECT is able to analyze urinary stones. The DECT is found superior especially in detecting uric acid and cystine stones. Its success in detecting hydroxyapatite and calcium oxalate stones is also high. When in vivo analyses of the stones are performed with DECT, it will be possible to make a contribution to the personalization and optimization of the treatment.Öğe Neutrophil gelatinase-associated lipocalin in prediction of mortality in patients with hepatorenal syndrome: a prospective observational study(WILEY, 2014) Gungor, Gokhan; Ataseven, Huseyin; Demir, Ali; Solak, Yalcin; Gaipov, Abduzhappar; Biyik, Murat; Ozturk, BahadirBackground & AimsHepatorenal syndrome (HRS) is a severe complication of cirrhosis which is characterized by renal dysfunction and associated with poor survival. Neutrophil gelatinase-associated lipocalin (NGAL) is a troponin-like biomarker for human acute kidney injury. We aimed to investigate levels of plasma and urine NGAL in HRS and predictive ability of these markers for all-cause mortality, in HRS, stable cirrhosis and control subjects. MethodsA total of 64 patients with cirrhosis (8 patients with type 1 HRS, 22 with type 2 HRS, and 34 without HRS) and 23 control subjects were included in the study. Blood and urine samples were measured with Human NGAL sandwich ELISA. Patients were followed up prospectively. ResultsPatients with type 1 and type 2 HRS had significantly higher plasma and urine NGAL levels compared with stable cirrhosis and control subjects. Cox regression analysis showed that plasma NGAL and MELD-Na scores were independent predictors of mortality. ROC-curve analysis showed that the plot of the plasma NGAL, urine NGAL, MELD-Na and Child-Turcot-Pugh score could predict all-cause mortality in cirrhotic patients' area under the curve (AUC 0.819, 0.686, 0.807 and 0.795 respectively). ConclusionsNGAL could predict mortality in patients with HRS independent of other commonly used risk factors.Öğe SERUM AND URINE NGAL LEVELS IN PATIENTS WITH CIRRHOSIS AND HEPATO-RENAL SYNDROME(OXFORD UNIV PRESS, 2012) Gungor, Gokhan; Ataseven, Huseyin; Demir, Ali; Solak, Yalcin; Biyik, Murat; Ozturk, Bahadir; Polat, Ilker[Abstract not Available]Öğe Serum oxidant and antioxidant status of patients with chronic tension-type headache: possible effects of medical treatment(SPRINGER-VERLAG ITALIA SRL, 2015) Cokal, Burcu Gokce; Aytac, Bilal; Durak, Zahide Esra; Gunes, Hafize Nalan; Ozturk, Bahadir; Guler, Selda Keskin; Durak, IlkerTension-type headache (TTH) is one of the most common and costly primary types of headache in clinical practice, with an unknown etiology. This study assessed to investigate oxidative and antioxidative status in patients with chronic tension-type headache (CTTH), and to evaluate possible effect of medical treatment. The study included 41 CTTH patients and 19 age- and sex-matched healthy subjects without headache as controls. The CTTH group comprised 20 patients receiving treatment and 21 untreated patients. We evaluated oxidant/antioxidant status by measuring serum malondialdehyde (MDA) levels and activities of antioxidant enzymes, namely glutathione peroxidase (GSH-Px) and catalase (CAT). Comparison of oxidative parameters in the patient and control groups revealed significantly lower CAT activities and higher MDA level and GSH-Px activities in the patient group. In the CTTH group, serum CAT activities were found to be significantly decreased in patient groups, while serum MDA levels and GSH-Px activities were found to be higher in the untreated CTTH patients. These findings suggest that oxidative stress is increased in the patients with CTTH, and medical treatment abolishes the stress in part. It has been concluded that antioxidant support might be helpful for the patients with CTTH to prevent oxidant stress and peroxidation damages further.Öğe Serum Prohepcidin and Hepcidin Levels in Patients with Ankylosing Spondylitis: A Prospective Study(DERMAN MEDICAL PUBL, 2016) Dagli, Mehmet; Yilmaz, Sema; Sivrikaya, Abdullah; Ozturk, BahadirAim: Anemia is a common complication in patients with inflammatory diseases such as ankylosing spondylitis. Recent data suggest that hepcidin is a major mediator of anemia with a central role in iron homeostasis and metabolism. The aim of this study was to evaluate the serum levels of hepcidin and its prohormone, prohepcidin, in patients with ankylosing spondylitis in comparison with healthy controls. Material and Method: Forty patients with ankylosing spondylitis (13 with anemia and 27 without anemia). 20 healthy adults were prospectively enrolled. Complete blood count, erythrocyte sedimentation rate, serum levels of hepcidin, prohepcidin, iron, total iron binding capacity, ferritin, transferrin, and C-reactive protein were measured. Results: Serum levels of prohepcidin and hepcidin were significantly higher in patients with ankylosing spondylitis compared to healthy controls (p<0.005). Positive correlation was determined between the serum hepcidin and prohepcidin levels in patients with ankylosing spondylitis (r=0.725, p <0.001). Discussion: To the best of our knowledge, this is the first report of serum levels of prohepcidin and hepcidin in the patients with ankylosing spondylitis. Serum levels of prohepcidin and hepcidin are closely associated with disease activity in patients with ankylosing spondylitis and might play a role in the pathogenesis of anemia of chronic disease associated with ankylosing spondylitis.Öğe Serum Prohepcidin and Hepcidin Levels in Patients withAnkylosing Spondylitis: A Prospective Study(2016) Dagli, Mehmet; Yilmaz, Sema; Sivrikaya, Abdullah; Ozturk, BahadirAmaç: Anemi, Ankilozan spondilit (AS) gibi enflamatuar hastalıklarda sık görülen bir komplikasyondur. Son veriler hepsidinin demir homeostaz ve metabolizmasında rol aldığını ve aneminin önemli bir aracısı olduğunu göstermektedir. Bu çalışmanın amacı, AS'li hastalarda ve sağlıklı kontrollerde serum hepsidin ve hepsidinin prohormonu olan prohepsidin düzeylerinin değerlendirilmesidir. Gereç ve Yöntem: Bu çalışmaya, AS'li kırk hasta (13 anemi olan ve 27 anemi olmayan) ile 20 sağlıklı katılımcı prospektif olarak alındı. Tam kan sayımı, eritrosit sedimantasyon hızı, serum hepsidin, prohepsidin, demir, total demir bağlama kapasitesi, ferritin, transferrin, ve C-reaktif protein düzeyleri ölçüldü. Bulgular: Serum prohepsidin ve hepsidin düzeyleri sağlıklı kontrol ile karşılaştırıldığında, AS'li hastalarda anlamlı olarak yüksek (p 0.005) bulundu. AS'li hastalarda, serum hepsidin ve prohepsidin düzeyleri arasında pozitif korelasyon ( 0.725 r, p 0.001) arasında saptandı. Tartışma: Bizim bilgilerimize göre bu çalışma AS'li hastalarda serum prohepsidin ve hepsidin düzeylerinin araştırıldığı ilk çalışmadır. AS'li hastalarda serum prohepsidin ve hepsidin düzeylerinin hastalığın aktivitesi ile yakından ilişkili olabileceği ve AS ile kronik hastalık anemisinin patogenezinde rol oynayabileceği düşünülmektedir.Öğe Static magnetic field inhibits 5 ' nucleotidase activity in cancerous and non-cancerous human gastric tissues(TAYLOR & FRANCIS INC, 2016) Durak, Zahide Esra; Buber, Suleyman; Kocaoglu, Ender Hilmi; Ozturk, BahadirThe aim of the present study is to investigate possible effects of static magnetic field (SMF) on 5 nucleotidase (5NT-CD73) and xanthine oxidase (XO) activities in cancerous and non-cancerous human gastric tissues in order to contribute to the elucidation of the anticancer activity of SMF. Cancerous and non-cancerous human gastric tissues removed from patients by surgical operations were used in the studies. SMF was created using two static magnets. Before and after treatment with SMF, 5NT and XO activities in the tissue samples were measured. 5NT activity was found to be lowered, but no significant change was observed in XO activity in the gastric tissues treated with the SMF. Our results suggest that SMF inhibits 5NT enzyme in gastric tissues significantly. It is supposed that in addition to other proposed mechanisms, inhibition of purine catabolic activity due to inhibition of some key enzymes in the DNA turn-over like 5NT might also play part in the anticancer activity of SMF.Öğe Static Magnetic Field Inhibits Adenosine Deaminase Activity in Cancerous and Noncancerous Human Gastric Tissues(MARY ANN LIEBERT, INC, 2014) Durak, Zahide Esra; Kocaoglu, Ender Hilmi; Ozturk, BahadirAim: Investigation of possible effects of static magnetic field (SMF) on adenosine deaminase (ADA) activity in cancerous and noncancerous human gastric and colon tissues to obtain information about possible action mechanism of SMF. Materials and Methods: Cancerous and noncancerous human gastric and colon tissues removed from patients by surgical operations were used in the studies. SMF was created by using two static magnets. Before and after treatment with SMF, ADA activities in the tissue samples were measured. Results: The ADA activity was found to be lowered in gastric tissues treated with the SMF. However, no change was observed in the ADA activity of colon tissues. Conclusions: Our results suggest that SMF inhibits the ADA enzyme in gastric tissues significantly. It is supposed that, in addition to other proposed mechanisms, accumulated adenosine due to the inhibition of the ADA enzyme might also play a part in the anticancer activity of SMF.
