Yazar "Ronci, Maurizio" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Comprehensive Chemical Profiling and Multidirectional Biological Investigation of Two Wild Anthemis Species (Anthemis tinctoria var. Pallida and A. cretica subsp. tenuiloba): Focus on Neuroprotective Effects
    (MDPI, 2019) Orlando, Giustino; Zengin, Gökhan; Ferrante, Claudio; Ronci, Maurizio; Recinella, Lucia; Şenkardeş, İsmail; Gevrenova, Reneta; Zheleva-Dimitrova, Dimitrina; Chiavaroli, Annalisa; Leone, Sheila; Di Simone, Simonetta; Brunetti, Luigi; Picot-Allain, Carene Marie Nancy; Mahomoodally, Mohamad Fawzi; Sinan, Kouadio Ibrahime; Menghini, Luigi
    Ethyl acetate (EA), methanol (MeOH), and aqueous extracts of aerial parts of Anthemis tinctoria var. pallida (ATP) and A. cretica subsp. tenuiloba (ACT) were investigated for their phenol and flavonoid content, antioxidant, and key enzyme inhibitory potentials. All extracts displayed antiradical effects, with MeOH and aqueous extracts being a superior source of antioxidants. On the other hand, EA and MeOH extracts were potent against AChE and BChE. Enzyme inhibitory effects against tyrosinase and alpha-glucosidase were observed, as well. We also studied Anthemis extracts in an ex vivo experimental neurotoxicity paradigm. We assayed extract influence on oxidative stress and neurotransmission biomarkers, including lactate dehydrogenase (LDH) and serotonin (5-HT), in isolated rat cortex challenged with K+ 60 mM Krebs-Ringer buffer (excitotoxicity stimulus). An untargeted proteomic analysis was finally performed in order to explore the putative mechanism in the brain. The pharmacological study highlighted the capability of ACT water extract to blunt K+ 60 mM increase in LDH level and 5-HT turnover, and restore physiological activity of specific proteins involved in neuron morphology and neurotransmission, including NEFMs, VAMP-2, and PKC gamma, thus further supporting the neuroprotective role of ACT water extract.
  • Küçük Resim
    Öğe
    Protective effects of Cotoneaster integerrimus on in vitro and ex-vivo models of H2O2-induced lactate dehydrogenase activity in HCT116 cell and on lipopolysaccharide-induced inflammation in rat colon
    (WILEY, 2019) Zengin, Gökhan; Ferrante, Claudio; Menghini, Luigi; Orlando, Giustino; Brunetti, Luigi; Recinella, Lucia; Chiavaroli, Annalisa; Leone, Sheila; Ronci, Maurizio; Aumeeruddy, Muhammad Zakariyyah; Mahomoodally, Mohamad Fawzi
    The present study evaluated the biological potential of methanol and aqueous extracts of the twigs and fruits of Cotoneaster integerrimus Medik. Lethality bioassays performed on Artemia salina showed that aqueous and methanol C. integerrimus extracts were non-toxic in the concentration range (0.1-20 mg/ml), with a LC50 >= 2.5 mg/ml, for each single extract. The protective effect of the extracts was assessed in vitro against hydrogen peroxide-induced lactate dehydrogenase (LDH) activity and tumor necrosis factor (TNF)alpha gene expression in colon cancer HCT116 cell line. All the extracts downregulated (H2O2)-induced TNF alpha gene expression, in HCT116. By contrast, it was observed that the lipopolysaccharide (LPS)-induced increase in colon nitrite, prostaglandin E-2, and 8-iso-PGF(2 alpha) levels were counteracted mostly by the methanol twig extract. The present study showed protective effects induced by C. integerrimus in vitro and ex vivo, thus supporting potential application in the management of chronic inflammatory diseases.