Protective effects of Cotoneaster integerrimus on in vitro and ex-vivo models of H2O2-induced lactate dehydrogenase activity in HCT116 cell and on lipopolysaccharide-induced inflammation in rat colon
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The present study evaluated the biological potential of methanol and aqueous extracts of the twigs and fruits of Cotoneaster integerrimus Medik. Lethality bioassays performed on Artemia salina showed that aqueous and methanol C. integerrimus extracts were non-toxic in the concentration range (0.1-20 mg/ml), with a LC50 >= 2.5 mg/ml, for each single extract. The protective effect of the extracts was assessed in vitro against hydrogen peroxide-induced lactate dehydrogenase (LDH) activity and tumor necrosis factor (TNF)alpha gene expression in colon cancer HCT116 cell line. All the extracts downregulated (H2O2)-induced TNF alpha gene expression, in HCT116. By contrast, it was observed that the lipopolysaccharide (LPS)-induced increase in colon nitrite, prostaglandin E-2, and 8-iso-PGF(2 alpha) levels were counteracted mostly by the methanol twig extract. The present study showed protective effects induced by C. integerrimus in vitro and ex vivo, thus supporting potential application in the management of chronic inflammatory diseases.
Açıklama
Anahtar Kelimeler
antioxidant, Cotoneaster integerrimus, inflammation, natural agents, oxidative stress
Kaynak
JOURNAL OF FOOD BIOCHEMISTRY
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
43
Sayı
3
Künye
Zengin, G., Ferrante, C., Menghini, L., Orlando, G., Brunetti, L., Recinella, L., Chiavaroli, A., Leone, S., Ronci, M., Aumeeruddy, M. Z., Mahomoodally, M. F. (2019). Protective Effects Of Cotoneaster İntegerrimus On İn Vitro And Ex‐Vivo Models Of H2O2‐İnduced Lactate Dehydrogenase Activity İn HCT116 Cell And On Lipopolysaccharide‐İnduced İnflammation İn Rat Colon. Journal Of Food Biochemistry, 43,(3), e12766.
