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Öğe Antibacterial and Antibiofilm Effects of Boron on Different Bacteria(HUMANA PRESS INC, 2016) Sayin, Zafer; Ucan, Uckun Sait; Sakmanoglu, AsliBoron (B) compounds are used in many fields ranging from medicine to industry. In this study, boric acid (BA) and disodium octaborate tetrahydrate (DOT) were evaluated for their antibacterial effects and antibiofilm capacities on selected strains of clinical and type cultures that are of veterinary concern (Staphylococcus aureus ATCC 25923, Aeromonas hydrophila ATCC 19570, Pseudomonas aeruginosa ATCC 27853, Brucella melitensis Rev1 and field isolates of Vibrio anguillarum, Aeromonas hydrophila, Yersinia ruckeri, Pseudomonas aeruginosa, Lactococcus garvieae, and Brucella abortus). Also, the inhibition of biofilm was monitored by scanning electron microscopy. The lowest MIC values of BA and DOT were measured, by broth method using microdilution, from Pseudomonas aeruginosa ATCC 27853, and were 0.385 and 0.644 mg/ml, respectively. Staphylococcus aureus was the most resistant to both BA and DOT. Using the microplate method, we observed that the strongest positivities for biofilm production were presented by Pseudomonas aeruginosa ATCC 27853 and also a clinical isolate of Lactococcus garviea. Lower values in the MIC scores for both B compounds were tested by measuring the inhibitory effect on biofilm production. We found that all the bacterial strains inhibited biofilm formation with the exception of the Pseudomonas aeruginosa strains for BA only and an isolate of Lactococcus garviea for DOT only. Such effects by BA and DOT are worth discussing in order to find novel approaches for different functions in medicine and industry using the bacteria tested.Öğe Bacillus anthracis Isolated from a Dog in Turkey(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2015) Sayin, Zafer; OzgurOzdemir; Erganis, Osman; Hadimli, Hasan Huseyin; Sakmanoglu, Asli; Atil, Eray; Sanioglu, GokcenurAn Anatolian Akbash shepherd dog died suddenly, without any clinical signs in Konya, Turkey. Performing necropsy, pathological examination, a culture test, laboratory tests and a multiplex-PCR of bacteria isolated from the dog revealed an anthrax and identified the bacteria as Bacillus anthracis (B. anthracis). The chromosomal gene sequence of bacteria was 99% identical in the GenBank under accession numbers CP002091 (B. anthracis str. H9401), CP001598 (B. anthracis str. A0248) and CP001215 (B. anthracis str. CDC684). Antimicrobial susceptibility test was performed and cefuroxime, cefquinome, sulfamethoxazole-trimethoprim, chloramphenicol, tetracycline and rifampicin resistance were seen in isolate. In this case, how the dog was infected with B. anthracis could not be determined. (C) 2014 PVJ. All rights reservedÖğe Comparison of five methods for isolation of DNA from Mycoplasma cynos(ELSEVIER SCIENCE BV, 2017) Sakmanoglu, Ash; Sayin, Zafer; Ucan, Uckun Sait; Pinarkara, Yasemin; Uslu, Ali; Erganis, OsmanExtraction of DNA from Mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. This eventually causes cell loss during harvesting of colonies from the agar surface. Here, we used the GenElute (TM) gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting DNA from Mycoplasma. We compared the DNA extraction efficiency of the GenElute (TM) gel extraction kit from Mycoplasma cynos cultured in agar medium with four other DNA extraction methods. The results were evaluated based on the purity and amount of DNA obtained from one Mycoplasma colony. Eight strains of Mycoplasma cynos isolated from the broncho-alveolar lavage fluid of dogs were used. The GenElute (TM) gel extraction protocol was the most efficient among all the methods tested in this study as it yielded the highest amount and the purest quality of DNA (199.3 +/- 0.744 ng/mu 1) from a single colony. Among the methods tested, the GenElute (TM) gel extraction method is the most rapid, sensitive, and simple method for DNA extraction from Mycoplasma. This procedure may also prove useful for extracting DNA from other Mycoplasma species.Öğe Comparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCR(UNIV ZAGREB VET FACULTY, 2016) Sayin, Zafer; Erganis, Osman; Sakmanoglu, Asli; Hadimli, Hasan H.; Pinarkara, Yasemin; Maden, Mehmet; Al Shattrawi, Huda J. G.In the present study, Pasteurella caballi (P. caballi) was isolated and identified in bronchoalveolar lavage fluid and lung samples from thoroughbred Arabian foals using conventional microbiological methods. Subsequently, the ability of two different PCR primer sets was evaluated for detection and confirmation of P. caballi. Primer sets 1 and 2, targeting the 16S rRNA gene of P. caballi, were designed using the Primer 3 and Primer-BLAST programs, respectively. PCR was performed to confirm P. caballi strains and to detect it directly in the bronchoalveolar lavage fluid and lung samples. In total, 35 Pasteurella spp. were isolated from 25 (38.4 %) of 65 bronchoalveolar lavage fluid samples, and 10 (58.8 %) of 17 lung samples. These strains were identified as P. caballi based on conventional microbiological and biochemical characteristics. The sensitivities of primers 1 and 2 were determined lobe 100 % to confirm cultured P. caballi strains. However, the specificity of P. caballi detection was lower with primer set-1 than primer set-2 in bronchoalveolar lavage fluid and lung samples. The sensitivity and specificity of primer set-2 were confirmed by gene sequence analysis. This study indicates that the 16S rRNA-PCR method, using primer set-2, provides a rapid and accurate tool for the detection and confirmation of P. caballi isolates in bronchoalveolar lavage fluid and lung samples from foals.Öğe The Effectiveness of Anti-R. equi Hyperimmune Plasma against R. equi Challenge in Thoroughbred Arabian Foals of Mares Vaccinated with R-equi Vaccine(HINDAWI LTD, 2014) Erganis, Osman; Sayin, Zafer; Hadimli, Hasan Huseyin; Sakmanoglu, Asli; Pinarkara, Yasemin; Ozdemir, Ozgur; Maden, MehmetThis study aimed to determine the effectiveness of a pregnant mare immunization of a Rhodococcus equi (R. equi) vaccine candidate containing a water-based nanoparticle mineral oil adjuvanted (Montanide IMS 3012) inactive bacterin and virulence-associated protein A (VapA), as well as the administration of anti-R. equi hyperimmune (HI) plasma against R. equi challenge in the mares' foals. The efficacy of passive immunizations (colostral passive immunity by mare vaccination and artificial passive immunity by HI plasma administration) was evaluated based on clinical signs, complete blood count, blood gas analysis, serological response (ELISA), interleukin-4 (IL-4) and interferon gamma (IFN-gamma), total cell count of the bronchoalveolar lavage fluids (BALF) samples, reisolation rate of R. equi from BALF samples (CFU/mL), lung samples (CFU/gr), and lesion scores of the organs and tissue according to pathological findings after necropsy in the foals. The vaccination of pregnant mares and HI plasma administration in the foals reduced the severity of R. equi pneumonia and lesion scores of the organs and tissue by 3.54-fold compared to the control foals. This study thus indicates that immunization of pregnant mares with R. equi vaccine candidate and administration of HI plasma in mares' foals effectively protect foals against R. equi challenge.Öğe Efficacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in mice(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2015) Erganis, Osman; Hadimli, Hasan Huseyin; Sayin, Zafer; Sakmanoglu, Asli; Pinarkara, Yasemin; Ozdemir, OzgurThe aim of this study was to determine the efficacy of inactive Rhodococcus equi vaccine candidates included that bacterin+aluminum hydroxide Al(OH)(3)), bacterin+VapA+(Al(OH)(3)), bacterin+Montanide IMS 3012 (IMS), bacterin+ VapA+IMS, and live vaccine using mice as a model. The efficacy of vaccine was evaluated according to clinical findings, humoral and cellular immunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrificed mice. Inactive R. equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injected once. Fifteen days after the last vaccination, aerosol challenges were carried out with the pathogenic R. equi VapA(+)K2002 strain in all groups. Two mice were sacrificed from each challenge groups on days 1, 3, 5, and 7. The antibody titers of vaccinated mice were found to be significantly higher than those of the controls. The largest number of INF-g positive samples were detected in the bacterin+ VapA+IMS and bacterin+ IMS groups. IL-4 positivity was determined only in live vaccine groups. The lowest reisolation rate of R. equi from internal organs was observed in the bacterin+VapA+IMS group. It was concluded that R. equi vaccines, and especially bacterin+VapA+IMS, are useful to protect mice against R. equi infection.Öğe Genotypic Characterization of Bordetella bronchiseptica Strains Isolated from Stray and Pet Dogs(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2016) Sayin, Zafer; Sakmanoglu, Asli; Erganis, Osman; Ucan, Uckun Sait; Hadimli, Hasan Huseyin; Aras, Zeki; Sanioglu, GokcenurBordetella bronchiseptica (B. bronchiseptica) is the most important pathogen associated with kennel cough in dogs. The presence of B. bronchiseptica in pet dogs and shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strains was determined to evaluate the role of stray dogs in spread of B. bronchiseptica to pet dogs by detection of virulence genes such as filamentous hemagglutinin (fha), pertactin (prn) and dermonecrotic toxin (dnt). We also performed the random amplified polymorphic DNA (RAPD) assay. A total of 96 B. bronchiseptica were isolated from stray and pet dogs. The fha, prn and dnt virulence genes were detected in 86, 83.3 and 61.4% strains, respectively by polymerase chain reaction (PCR) techniques. The most common genotype from stray and pet dogs was fha+prn+dnt+ as detected in 37.5% and 11.4% of all the strains, respectively. The RAPD assay showed that 3 different patterns were obtained from 96 B. bronchiseptica strains. Sixty one (63.5%) of them were clustered in one main group and then further placed in another 2 sub-groups by RAPD assay. Genetic association was seen between the B. bronchiseptica strains from stray and pet dogs. In conclusion, this study revealed that B. bronchiseptica is present at a higher rate in stray dogs than pet dogs. Stray dogs might have a significant role in the transmission of B. bronchiseptica to pet dogs. (C)2016 PVJ. All rights reservedÖğe IDENTIFICATION OF MYCOBACTERIUM STRAINS BY PCR AND PCR-REA(NATL VETERINARY RESEARCH INST, 2011) Sayin, Zafer; Erganis, OsmanThe presented study aimed isolation of Mycobacterium strains from cases of bovine tuberculosis in Turkey, and identification of these isolates by PCR and restriction endonuclease analysis (REA). Mycobacteria were isolated from bronchial, mesenteric, and prescapular lymph nodes and tubercle samples from slaughtered cattle, which were either skin-test reactors or showed tuberculous suspected lesions during meat inspection. The samples were cultured in Lowenstein-Jensen medium. Colonies suspected of being Mycobacterium sp. were analysed by the PCR method using the MTUB_c-gyrBf and MTUB_c-gyrBr primers, which amplify the 1,020-bp region of the gyrB gene, that encodes the B subunit of the DNA gyrase (topoisomerase) enzyme in strains of the Mycobacterium tuberculosis complex (MTBC). The REA of MTBC-PCR positive amplified products was performed using the Sacl1 and Rsa1 enzymes, in order to identify specific strains. The MTBC-PCR analysis of 117 bacterial strains demonstrated that all isolates belonged to the MTBC. All isolates were identified as M bovis/M bovis subsp. caprae by Rsa1-REA, whilst 93 (79.5%) of the bacterial strains were identified as M. bovis and 24 (20.4%) as M. bovis subsp. caprae by Sacll-REA. M. bovis subsp. caprae was isolated and identified for the first time in Turkey from bovine tuberculosis cases.Öğe Isolation of arcanobacterium pyogenes from samples of sheep and cattle and identification by polimerase chain reaction(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2010) Hadimli, H. Hueseyin; Erganis, Osman; Kav, Kuersat; Sayin, ZaferThe aims of this study were to isolate Arcanobaterium pyogenes (A. pyogenes) from cattle and sheep, to identify the isolates by conventional methods, Polymerase Chain Reaction (PCR) and to determine antimicrobial susceptibilities. In this study, a total 716 samples was collected from sheep and cattle (liver, milk, broncho alveoler lavage, lung, suppurative tissue, and fluid of joint). All samples were cultured onto blood agar base containing 5% defibrinated sheep blood and plates were incubated at 37 degrees C for 48 h. Total 51 A. pyogenes strains isolated from samples were identified in according to properties of biochemical. Then, PCR analyses were made using specific primers for A. pyogenes. All isolates were also confirmed by PCR. In addition, antimicrobial activities were determined to 16 antimicrobial agents, some of which were used in veterinary medicine. There is emphasized that this bacterium may cause a variety of infections with economically losses in sheep and cattle.Öğe Molecular epidemiology of mycoplasma synoviae infection in commercial layers(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2014) Aras, Zeki; Sayin, ZaferMycoplasma synoviae (M. synoviae) infection is a cause of great economic loss in commercial egg layer hens. The aims of this study were to investigate the prevalence of M. synoviae and to compare the characteristics of M. synoviae infected and free flocks of commercial layers. A total of 400 tracheal swabs and 400 blood serum samples were collected from 20 different layer flocks. Random Amplified Polymorfic DNA (RAPD) analysis was used for the molecular typing of M. synoviae isolates to determine the source of infection. M. synoviae was isolated from 89 tracheal swabs collected from 5 out of 20 flocks. The genetic similarity between field strains ranged from 53% to 100% as determined by RAPD analysis. All M. synoviae strains separated into 2 main-clusters in UPGMA dendogram. These results revealed that the 5 infected flocks were contaminated from 2 different sources. The egg production of positive flocks was statistically lower (P<0.05) than that of the pathogen free flocks. Infection was more frequent in multi-age farms and on sites with several houses. The mortality of infected flocks was higher than uninfected flocks, but this difference was not statistically significant. The mean weight of eggs and the average live weight of hens were similar in free and infected flocks. In conclusion, M. synoviae infection significantly decreased egg production in layer hens and was more frequent in multiage farms.Öğe Mycoplasma infections in dairy cattle farms in Turkey(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Sayin, Zafer; Sakmanoglu, Asli; Ucan, Uckun Sait; Uslu, Ali; Hadimli, Hasan Huseyin; Aras, Zeki; Ozdemir, OzgurMycoplasmas cause the most severe and economically costly diseases of cattle throughout the world. In this study, Mycoplasma species were isolated from calves and cows with suspected mycoplasmosis in Holstein dairy cattle farms within 7 geographical regions of Turkey between May 2010 and December 2015. Mycoplasma infections were positive in 17 (80.9%) of 21 dairy cattle farms and the overall percentage was calculated as 32.1%. The highest isolation rate occurred in the Southeastern Anatolia Region (42.8%), and the lowest was observed in the Mediterranean Region (19.6%). In total, 172 Mycoplasma spp. were isolated from samples. Using PCR analysis, 149 (87.6%) isolates were identified as Mycoplasma bovis (M. bovis). Eleven (6.3%) isolates were identified as M. alkalescens, 2 (1.1%) were M. canis, and 10 (5.8%) were M. bovigenitalium. The isolation rate was found to be increasing annually. In conclusion, mycoplasmosis is a common problem in Holstein dairy cattle farms in Turkey, and M. bovis is the most frequently encountered cause of mycoplasma infections. The isolation rate seems to have increased in correlation with increased live cattle imports. Additionally, M. alkalescens and M. canis were isolated and identified in respiratory tract infections in cattle from Turkey for the first time.Öğe Production and development of vaccines for ornithobacterium rhinotracheale infection in turkeys(2010) Erganis, Osman; Hadimli, Hasan Hüseyin; Kav, Kursat; Sayin, Zafer; Aras, ZekiErganiş O, Hadimli HH, Kav K, Sayın Z, Aras Z. Hindilerde Ornithobacterium rhinotracheale için aşıların geliştirilmesi ve üretilmesi. Eurasian J Vet Sci, 2010, 26, 2, 101-107 Amaç: Bu çalışmanın amacı bivalan inaktif Ornithobacterium rhinotracheale aşıları hazırlamak, kan serumlarında antijenlere karşı antikorların titrelerini ölçmek ve hindilerde O. rhinotracheale aşılarının etkinliklerini belirlemektir. Gereç ve Yöntem: Bivalan inaktif O. rhinotracheale aşıları; alüminyum hidroksit, mineral yağlı, alüminyum hidroksit ginseng ve mineral yağ ginseng adjuvantları kullanılarak O. rhinotracheale serotip A ve B’den hazırlandı. Sterilite ve zararsızlık testlerinden sonra, hindilerde (5. ve 8. haftalarda 0.25ml ve 0.5 ml dozlarla iki kez aşılama ile) aşıların laboratuvar etkinlikleri (çelınç/koruma ve serolojik potens) yapıldı. Bulgular: Çelınç sonuçlarına göre hindilerde bütün aşıların %100 etkili olduğu bulundu. Aşılı ve aşısız grupların serumlarında titrelerin serolojik ölçümleri için ve saha şartlarında O. rhinotracheale enfeksiyonunun teşhisinde lam aglütinasyon, mikro serum aglütinasyon ve ELISA testleri kullanıldı. Adjuvant olarak mineral yağ ve ginseng içeren aşı diğerlerine göre belirgin olarak daha yüksek humoral immune cevap oluşturdu. Aynı zamanda ve mineral yağ ginseng aşısı özel bir hindi işletmesinde saha denemesinde çok etkili olduğu belirlendi. Öneri: Kanatlılarda ornitobakteriozisin önlenmesi için O. rhinotracheale aşıları kullanılabilir.Öğe The protective efficacy of immunoglobulin Y from immunized chickens against Salmonella infections in mice(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2017) Hadimli, Hasan Huseyin; Sayin, Zafer; Sanioglu Golen, GokcenurThe aim of this study was to determine the efficacy of immunoglobulin Y (IgY) obtained from chickens immunized with Salmonella vaccines. Chickens were vaccinated three times with inactivated monovalent, bivalent, and combined vaccines. Immunized hen eggs were collected after the third vaccination and IgYs were purified. In total, 100 mice were orally challenged with Salmonella serotypes. After the challenge, IgYs were orally administered to mice. Mice were observed for morbidity and mortality. Fecal samples from the mice were also cultured for the reisolation of Salmonella serotypes. The antibody titers in the serum samples of vaccinated chickens were higher than those of controls (P < 0.001). Neither morbidity nor mortality were observed in these mice. In all of the groups the reisolation numbers of the Salmonella serotypes from internal organs and fecal samples were low ( P < 0.001) In conclusion, it is suggested that IgYs from immunized chickens could be used to establish protection against Salmonella infections.Öğe Sero-Epidemiology of the Rhodococcus Equi in Horses in Eastern Kazakhstan(Selçuk Üniversitesi, 2023 Haziran) Uslu, Ali; Sayin, Zafer; Balevi, Aslı; Ilban, Aysegul; Erganis, Osman; Yerken, Kassymov; Bauyrzhan, Otarbayev; Yerbol, Ismagulov; Gulnaz, IlgekbayevaAmaç: Bu çalışma Doğu Kazakistan'daki yetişkin atlarda Rhodococcus equi (R. equi) sero-prevalansını değerlendirmek için tasarlanmıştır. Gereç ve Yöntem: Eylül ve Kasım 2021 tarihleri arasında doğu Kazakistan’da bulunan üç bölgede (Zhambyl, Almatı, Doğu Kazakistan) 311 attan serum toplandı ve virülent R. equi'ye karşı oluşmuş olan antikorların varlığı enzim bağıntılı immünosorbent test (ELISA) ile tespit edildi. Bulgular: R. equi'nin doğu Kazakistan’da bulunan at popüslayonlarında ki seroprevalansı 2021 yılında %93,6 olarak bulundu. En yüksek seroprevalans Vap A oranı Zhambly Bölgesi'nde (%98,02), en düşük ise Doğu Kazakistan Bölgesi'nde (%88,89) görüldü. Bu bölgeden alınan örnekler toplam örneklerin %69,8'ini oluşturmakta olup, %3,84'ü vapA seronegatiftir. R. equi’nin Doğu Kazakistan'da endemik enfeksiyona sebep olduğu tespit edildi.. Öneri: Bu, R. equi'nin Kazakistan'da epidemiyolojisi hakkında bilgi sağlayan ilk sero survey çalışmasıdır. Serolojik bulgular klinik vakalar ve tay tavlalarında bakteri izolasyonu ile desteklenmelidir. Doğu Kazakistan'da R. equi'ye karşı koruma ve kontrol programları uygulanmalıdır.Öğe Serologic prevalence of Ornithobacterium rhinotracheale infection in commercial layers(2016) Aras, Zeki; Sayin, Zafer; Sanioglu, Gokcenur. Amaç: Bu çalışmada ticari yumurtacı tavuk işletmelerinde bulunan tavuklarda Ornithobacterium (O.) rhinotracheale enfeksiyonunun serolojik prevalansının belirlenmesi amaçlandı.Gereç ve Yöntem: Konya, Aksaray, Karaman, Ankara ve Gaziantep illerinde bulunan 26 farklı kümesteki yumurtacı tavuklardan 650 kan serum örneği toplandı. Örnekleme yapılan tavukların hiç birisi daha önceden O. rhinotracheale enfeksiyonuna karşı aşılanmamışlardı. O. rhinotracheale'ye karşı oluşmuş antikorların varlığı ELISA testi ile belirlendi.Bulgular: Toplam 650 kan serum örneğinden 113 (%17.4)'ünde O. rhinotracheale antikoru tespit edildi. Bu 113 pozitif serum örneği örnekleme yapılan 26 kümesin 12 (%46.2)'sinden toplanmıştı. Enfekte 12 kümes, Konya, Gaziantep, Ankara ve Karaman illerindeki 12 farklı çiftliğe aitti. Öneriler: Bu çalışmanın sonuçları göstermiştir ki, Türkiye'deki ticari yumurtacı tavuk kümeslerinde O. rhinotracheale enfeksiyonu yüksek bir prevalansa sahiptir.Öğe Serosurveillance of&IT Neospora caninum&IT and &ITBrucella&IT species in Dairy Cattle of Konya, Turkey(FRIENDS SCIENCE PUBL, 2018) Ekici, Ozlem Derinbay; Isik, Nermin; Sayin, Zafer; Coskun, Alparslan; Sajid, Muhammad SohailThis study reports the seroprevalence of Neospora (N.) caninum and Brucella sp. in aborting and non-aborting daily cattle in Konya province of Turkey. To this end, blood samples were collected from 560 cattle, 66 of which were not aborting and 494 were aborting, and sera were isolated from these samples through standard protocol. Antibodies against N. caninum were determined by using a commercial competitive ELISA (cELISA) kit. Brucella sp. antibodies were determined using the Rose Bengal Plate Test (RBPT). According to cELISA results, 222 of 560 cattle (39.64%) were seropositive for N. caninum antibodies. Of 494 aborting cattle samples, 213 (43.11%) were positive for N. caninum antibodies. Through RBPT, 89 of the 560 cattle tested were positive for Brucella sp. Of 494 aborting cattle, 79 (15.99%) were positive for Brucella sp. The seropositivity differences between N. caninum and Brucella sp. were statistically significant in aborting cattle (p< 0.001). The co-infection rate of N. caninum seropositivity with B. abortus was detected 9.5% in aborting cattle. In conclusion, seroprevalence of neosporosis and Brucella sp. was 39.64% and 15.89% through cELISA and RBPT, respectively in cattle of Konya. (C) 2018 Friends Science PublishersÖğe Serotypes of Salmonella isolated from feces of cattle, buffalo, and camel and sensitivities to antibiotics in Turkey(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2017) Hadimli, Hasan Huseyin; Pinarkara, Yasemin; Sakmanoglu, Asli; Sayin, Zafer; Erganis, Osman; Uslu, Ali; Al-Shattrawi, Huda JihadIn this study, Salmonella strains from dairy cows, calves with diarrhea, and buffalo and camel feces were isolated and serotyped. There were 869 feces samples (437 calves, 287 dairy cows, 100 buffalo, and 45 camels) collected from 13 provinces and 21 farms. Preenrichment feces samples were added to Rappaport-Vassiliadis medium. Samples were then subcultured on xylose lysine tergitol-4 agar and plates. Suspected colonies of Salmonella were confirmed by latex agglutination test. The isolates of Salmonella spp. were serotyped at the Etlik Central Veterinary Control Institute. In total, 40 Salmonella spp. were isolated, including 33 from calves, 5 from dairy cows, 1 from a buffalo, and 1 from a camel. Salmonella strains were serotyped as S. Kentucky (n = 23), S. Muenchen (n = 5), S. Anatum (n = 4), S. Gaminare (n = 4), S. Typhimurium (n = 1), S. Muenster (n = 1), S. Enteritidis (n = 1), and S. Abony (n = 1). The Kirby-Bauer disk diffusion method was used for the determination of antibiotic susceptibilities of isolates against 15 antibiotics. Salmonella isolates were determined as resistant to multiple antibiotics. In conclusion, 8 different Salmonella serotypes were found in dairy cows, calves, buffalo, and camels. Because of the occurrence of multiresistant isolates, biosafety measures and pathogen control processes are advised for Salmonella-associated risks to public health.