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Öğe Bond strength of two total-etching bonding systems on caries-affected and sound primary teeth dentin(WILEY, 2008) Tosun, Gul; Koyuturk, Alp Erdin; Sener, Yagmur; Sengun, AbdulkadirAim. As bond strength of currently available adhesive systems in caries-affected teeth dentin on primary tooth dentin was not well known, the bond strength of two bonding systems (PQI and OptiBond Solo Plus) was evaluated on caries-affected and sound primary molar tooth dentin and observed the micromorphology of the adhesive-dentin interfaces. Methods. By grinding both the sound (n = 30) and caries-affected (n = 30) approximal surfaces of teeth, flat dentin surfaces were obtained. The prepared surfaces were bonded with one of the each adhesive systems and a composite resin. After storing the bonded specimens in water at 37 degrees C for 24 h, the samples were sectioned and the bond strength of the adhesive systems was tested by the microshear test method. The data were statistically analysed. Results. Microshear bond strengths of PQI group for caries-affected and sound primary tooth dentin were 9.43 +/- 2.44 (MPa) and 9.32 +/- 2.95 (MPa) (P > 0.05), respectively, and the bond strengths of OptiBond Solo Plus group for caries-affected and sound primary tooth dentin were 15.33 +/- 3.59 (MPa) and 11.96 +/- 2.30 (MPa) (P < 0.05), respectively. Micromorphological features between sound and caries-affected dentin were similar in both PQI and OptiBond Solo Plus groups. Conclusion. Both the adhesives showed significantly different bond strengths in caries-affected dentin but showed similar bond strengths in sound dentin.Öğe Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells(MOSBY-ELSEVIER, 2011) Sengun, Abdulkadir; Yalcin, Muhammet; Ulker, Hayriye Esra; Ozturk, Bora; Hakki, Sema S.Objective. The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells. Study design. Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into 2 compartments using a 500 mu m dentin disk. The 3D cultures were placed on a dentin disk and held in place with a special biocompatible stainless steel holder. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin disks according to the manufacturer's instructions. Subsequently, the pulpal part of the perfusion chamber containing the cell cultures was perfused with a medium (2 mL/h). After an exposure period of 24 hours, cell survival was determined by using the MTT assay. Statistical analyses were performed using the Mann-Whitney U test. Results. In the dentin barrier test, cell survival rates of UB and CDCB were similar to the control group (P > .05). However, all other tested materials were cytotoxic for the 3D pulp-derived cell cultures (P > .05). Conclusions. Dentin-bonding agents include biologically active ingredients and may modify pulp cell metabolism when the materials are used in deep cavities in spite of a dentin barrier. If these adhesive agents are used in deep cavities, a biocompatible cavity liner should be used. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: e83-e88)Öğe Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis(JAPANESE SOC DENTAL MATERIALS DEVICES, 2015) Malkoc, Meral Arslan; Demir, Necla; Sengun, Abdulkadir; Bozkurt, Serife Buket; Hakki, Sema SezenTo evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs' index at 24 and 72 h (p <= 0.001). These materials were found to be the most toxic resin cements, as compared to the control and other tested materials (C2 and MA). However, C2 and MA showed a better survival rate, compared to BQM, RU200, and ME, and had lower cell index than the control group. The cytotoxic effects of resin cements on pulpa should be evaluated during the selection of proper cements.Öğe Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis(KOREAN ACAD PROSTHODONTICS, 2015) Malkoc, Meral Arslan; Demir, Necla; Sengun, Abdulkadir; Bozkurt, Serife Buket; Hakki, Sema SezginPURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 mu L of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.Öğe Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells(HINDAWI PUBLISHING CORPORATION, 2013) Ulker, Hayriye Esra; Ulker, Mustafa; Gumus, Hasan Onder; Yalcin, Muhammet; Sengun, AbdulkadirThis study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P < 0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P > 0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.Öğe Effect of a new restoration technique on fracture resistance of endodontically treated teeth(BLACKWELL PUBLISHING, 2008) Sengun, Abdulkadir; Cobankara, Funda Kont; Orucoglu, HasanThe aim of the study was to investigate the effect of a new fiber-reinforced composite restoration technique on fracture resistance in endodontically treated premolars. Eighty sound extracted human mandibular premolars were assigned to four groups (n = 20). Group 1 did not receive any treatment. In groups 2, 3 and 4, the teeth received root canal treatment and a mesio-occluso-distal cavity preparation. Group 2 was kept unrestored. Group 3 was restored with a dentin bonding system and composite resin. In group 4, a piece of polyethylene ribbon fiber was inserted into the groove in a buccal to lingual direction during the restoration of teeth with dentin bonding system and composite resin. After finishing and polishing, the specimens were stored in 100% humidity at 37 degrees C for 24 h and placed at an angle of 45 degrees to the long axis of the tooth and subjected to compressive loading in a universal testing machine at a crosshead speed of 0.5 mm min(-1). The load necessary to fracture the samples was recorded in Newton (N) and submitted to Kruskal-Wallis ANOVA and Mann-Whitney U-test. The fracture strength of the teeth reinforced with a combination of polyethylene fiber and composite resin were not significantly different than those that were restored with only composite resin (P > 0.05). However, most of the failure modes of the reinforced teeth were limited to the level of the enamel, while the other three groups showed fractures generally at the level of the dentin, cemento-enamel junction or more below (P < 0.05). Therefore, polyethylene ribbon fiber-reinforced composite resin restorations seemed a more reliable restorative technique than traditional composite restorations for extensive cavities.Öğe Effect of accelerated aging on the bonding performance of fluoridated adhesive resins(JAPANESE SOC DENTAL MATERIALS DEVICES, 2014) Cobanoglu, Nevin; Ozturk, Bora; Sengun, Abdulkadir; Cetin, Ali Riza; Ozer, FusunThe purpose of this study was to investigate the dentin bond durability of a one-step, fluoride-containing, glass ionomer-based adhesive system, Reactmer Bond (RB), and that of a two-step, fluoride-containing, self-etch adhesive system, Clearfil Protect Bond (CPB). Enamel was removed from the occlusal surfaces of teeth, and flat dentin surfaces were entirely covered with a composite resin following the application of an adhesive material (n=10). After specimens were sectioned into rectangular sticks of 0.87 +/- 0.03 mm(2), the sticks were randomly assigned into two accelerated aging time period groups: 1 week or 1 year. Microtensile bond strengths were determined. Bond strength of RB increased significantly after 1 year (1 week=27.80 +/- 10.57 MPa versus 1 year=36.93 +/- 14.38 MPa) (p<0.05). In contrast, there was no significant difference in bond strength between the two time periods for CPB (1 week=51.74 +/- 17.8 MPa versus 1 year=56.03 +/- 18.85 MPa) (p>0.05). Both fluoride-containing adhesives seemed to demonstrate reliable bonding performance after 1 year of accelerated aging in water.Öğe Effect of storage duration/solution on microshear bond strength of composite to enamel(JAPANESE SOC DENTAL MATERIALS DEVICES, 2007) Tosun, Gul; Sener, Yagmur; Sengun, AbdulkadirThe aim of this study was to determine the effect of three storage solutions and two storage durations on microshear bond strength (mu SBS) of a resin composite. Sixty non-carious human permanent molars were stored in three storage solutions (0.1% thymol, 10% formalin, and distilled water). Each tooth was separated mesio-distally into two parts. Specimens of the first part were stored for 24 hours, while specimens of the second part were stored for two months in the solutions. After each storage period, the enamel surface was covered with a composite resin in combination with an etch-rinse adhesive system. Specimens were then serially sectioned into sticks of I mm(2) bond area and subjected to mu SBS test. There were no statistically significant differences between the two storage periods for each solution (p > 0.05). The thymol solution group showed lower mu SBS values than those of distilled water for both storage periods (p < 0.05). As for the formalin group, its mu SBS values were not statistically different from those of distilled water and thymol groups at each storage period (p > 0.05). In conclusion, the thymol solution caused the mu SBS of the resin composite to decrease when compared to both formalin and distilled water after 24 hours and two months. However, the mu SBS of the resin composite was not affected by storage duration.
