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Öğe Chemical Composition of a New Taxon, Seseli gummiferum subsp ilgazense, and its Larvicidal Activity against Aedes aegypti(ACG PUBLICATIONS, 2018) Kurkcuoglu, Mine; Tabanca, Nurhayat; Ali, Abbas; Khan, Ikhlas A.; Duran, Ahmet; Baser, K. Husnu CanMosquitoes are vectors for many pathogens and parasites that cause human diseases including dengue, yellow fever, West Nile, chikungunya, filariasis and malaria which cause high rates of human morbidity and mortality under extreme conditions. Plants are an excellent source for mosquito control agents because they constitute rich sources of bioactive chemicals. They are also biodegradable and environment-friendly. The present study reports on the larvicidal activity of the essential oil of Seseli gummiferum. subsp. ilgazense (Apiaceae) against Aedes aegypti larvae. Essential oil showed 100 and 70% mortality at 125 and 62.6 ppm, respectively, with no mortality at 31.25 ppm. Aerial parts of S. gummiferum subsp. ilgazense were subjected to hydrodistillation to yield 0.6% oil. The essential oil was analyzed by GC-FID and GC-MS techniques. The main constituents in the oil were sabinene (28.8%), germacrene D (9.5%) and alpha-pinene (7.2%).Öğe Chemical Composition, Larvicidal, and Biting Deterrent Activity of Essential Oils of Two Subspecies of Tanacetum argenteum (Asterales: Asteraceae) and Individual Constituents Against Aedes aegypti (Diptera: Culicidae)(ENTOMOLOGICAL SOC AMER, 2014) Ali, Abbas; Tabanca, Nurhayat; Kurkcuoglu, Mine; Duran, Ahmet; Blythe, Eugene K.; Khan, Ikhlas A.; Baser, K. Husnu CanWater-distilled essential oils from dried aerial parts of Tanacetum argenteum (Lam.) Willd. subsp. argenteum (Lam.) and T. argenteum (Lam.) Willd. subsp. canum (C. Koch) Grierson were analyzed by gas chromatography and gas chromatography-mass spectrometry. In total, 27 and 32 components were identified representing 97.2 and 98.7% of essential oils of subsp. argenteum and canum, respectively. Main compounds of T. argenteum subsp. argenteum were alpha-pinene (67.9%) and beta-pinene (4.8%), whereas alpha-pinene (53.6%), 1, 8-cineole (14.8%), and camphor (4.7%) were the major constituents of subsp. canum. Essential oil of T. argenteum subsp. canum at 10 mu g/cm(2) with Biting Deterrent Index (BDI) value of 0.73 showed activity similar to N, N-Diethyl-meta-toluamide (DEET) at 25 mol/cm(2), whereas the activity of essential oil of subsp. argenteum was lower (BDI = 0.47) than subsp. canum and DEET. Based on 95% CIs, activity of beta-caryophyllene (BDI value = 0.54) and caryophyllene oxide (BDI = 0.66) were significantly lower than DEET. In larval bioassays, essential oil of T. argenteum subsp. argenteum showed LC50 value of 93.34 ppm, whereas T. argenteum subsp. canum killed only 40% of the larvae at the highest dose of 125 ppm. Among the pure compounds, beta-caryophyllene (LC50 = 26 ppm) was the most potent compound followed by caryophyllene oxide (LC50 = 29 ppm), which was also similar to (-)-beta-pinene (LC50 = 35.9 ppm) against 1-d-old Ae. aegypti larvae at 24-h post treatment. Compounds (-)-alpha-pinene and (+)-beta-pinene showed similar larvicidal activity. Activity of (+)-alpha-pinene with LC50 value of was similar to the essential oil of T. argenteum subsp. argenteum.Öğe Gas Chromatographic-mass Spectrometric Analysis of Volatiles Obtained by Four Different Techniques From Salvia Rosifolia Sm., and Evaluation for Biological Activity(Elsevier, 2010) Özek, Gülmira; Demirci, Fatih; Özek, Temel; Tabanca, Nurhayat; Wedge, David E.; Khan, Shabana I.; Başer, Kemal Hüsnü Can; Duran, Ahmet; Hamzaoğlu, ErginFour different isolation techniques, conventional hydrodistillation (HD), microwave-assisted hydrodistillation (MWHD), microdistillation (MD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the aerial parts of Salvia rosifolia Sm. by gas chromatography and gas chromatography coupled to mass spectrometry. HD and MWHD techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar essential oils. alpha-Pinene (15.7-34.8%), 1,8-cineole (16.6-25.1%), beta-pinene (6.7-13.5%), beta-caryophyllene (1.4-5.0%) and caryophyllene oxide (1.4-4.4%) were identified as major constituents of this Turkish endemic species. Besides, the hydrodistilled oil of S. rosifolia was evaluated for antibacterial, antifungal, anticancer, antioxidant and cytotoxic activities. The hydrodistilled oil of S. rosifolia showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) with a MIC value of 125 mu g/mL Other human pathogenic microorganisms (Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus epidermidis, Candida albicans) were also inhibited within a moderate range (MIC = 125-1000 mu g/mL). Antifungal activity of the oil was also observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. No cytotoxicity was observed for S. rosifolia oil up to 25 mg/mL against malignant melanoma, epidermal, ductal and ovary carcinoma.Öğe The Identification of Suberosin from Prangos pabularia Essential Oil and Its Mosquito Activity Against Aedes aegypti(ACG PUBLICATIONS, 2016) Tabanca, Nurhayat; Tsikolia, Maia; Ozek, Gulmira; Ozek, Temel; Ali, Abbas; Bernier, Ulrich R.; Duran, AhmetA detailed analysis of Prangos pabularia Lindl. (Apiaceae) fruit oil was performed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Bicyclogermacrene (21%), (Z)-beta-ocimene (19%), alpha-humulene (8%), alpha-pinene (8%) and spathulenol (6%) were the main constituents of the oil. One compound with 1.8% at RI 3420 remained unidentified or tentatively identified as suberosin from the Wiley GC-MS Library. The assumed compound, suberosin was synthesized in two steps and its structure was confirmed by 1D NMR and GC-MS analyses. As part of our continued research to discover new chemicals for use in mosquito control agents as repellents and larvicides, suberosin and its parent compound coumarin were investigated for the mosquito biting deterrent and larvicidal activity against Aedes aegypti. Both suberosin and coumarin showed biting deterrent activity but the activity was lower than the positive control, DEET (N,N-diethyl-3-methylbenzamide). In larval bioassays, suberosin with LC50 value of 8.1 ppm was significantly more toxic than coumarin (LC50 = 49.6 ppm) at 24-h post treatment. These results indicate that suberosin may be useful for use as mosquito larvicidal agent.