Gas Chromatographic-mass Spectrometric Analysis of Volatiles Obtained by Four Different Techniques From Salvia Rosifolia Sm., and Evaluation for Biological Activity
Yükleniyor...
Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Four different isolation techniques, conventional hydrodistillation (HD), microwave-assisted hydrodistillation (MWHD), microdistillation (MD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the aerial parts of Salvia rosifolia Sm. by gas chromatography and gas chromatography coupled to mass spectrometry. HD and MWHD techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar essential oils. alpha-Pinene (15.7-34.8%), 1,8-cineole (16.6-25.1%), beta-pinene (6.7-13.5%), beta-caryophyllene (1.4-5.0%) and caryophyllene oxide (1.4-4.4%) were identified as major constituents of this Turkish endemic species. Besides, the hydrodistilled oil of S. rosifolia was evaluated for antibacterial, antifungal, anticancer, antioxidant and cytotoxic activities. The hydrodistilled oil of S. rosifolia showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) with a MIC value of 125 mu g/mL Other human pathogenic microorganisms (Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus epidermidis, Candida albicans) were also inhibited within a moderate range (MIC = 125-1000 mu g/mL). Antifungal activity of the oil was also observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. No cytotoxicity was observed for S. rosifolia oil up to 25 mg/mL against malignant melanoma, epidermal, ductal and ovary carcinoma.
Açıklama
Anahtar Kelimeler
Salvia rosifolia, Essential oil, Hydrodistillation, Microwave, Microdistillation, Solid-phase microextraction, alpha-Pinene, 1,8-Cineole, Biological activity
Kaynak
Journal of Chromatography A
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
1217
Sayı
Künye
Özek, G., Demirci, F., Özek, T., Tabanca, N., Wedge, D. E., Khan, S. I., Başer, K. H. C., Duran, A., Hamzaoğlu, E., (2010). Gas Chromatographic-mass Spectrometric Analysis of Volatiles Obtained by Four Different Techniques From Salvia Rosifolia Sm., and Evaluation for Biological Activity. Journal of Chromatography A, (1217), 741-748. Doi: 10.1016/j.chroma.2009.11.086
