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Öğe The application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2011) Arslan, Emine; Tamkoc, AhmetThe present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be the most suitable for distinguishing between species. Because interspecies differences are especially important in plant breeding, M5 and UBC812 primers were ideal in distinction of P. trivialis and P angustifolia accessions, respectively. Primer UBC856 was determined to be ideal in discrimination both intraspecies and interspecies. The ISSR fingerprinting technique used was confirmed to be a reproducible and sensitive tool for the identification of Poa accessions and for determination of the correct starting material for plant breeding.Öğe Correlation Between Traits and Path Analysis For Seed Yield in Cephalaria syriaca Lines(SOC PLANT RESEARCH, 2013) Ada, Rahim; Tamkoc, Ahmet; Karahan, YahyaThis research was conducted to determined correlation among seed yield with their components and through the path coefficient analysis method, an experiment with 33 Cephalaria syriaca lines and as control, 1 population at Konya-Turkey, in 2010 and 2011. Seed yield revealed in p<0.01 level significant positive association with plant height, head number and oil yield. Also, The highest positive direct effect of oil yield on seed yield was observed followed by plant height and head number.Öğe Determination of Chromosome Structures of Genotypes of Perennial Ryegrass (Lolium perenne L.) Selected from Natural Vegetation of Turkey(UNIV TOKYO CYTOLOGIA, 2018) Ozer, Irfan; Tamkoc, Ahmet; Uysal, TunaThe scope of the present study is to contribute to future breeding studies by determining the relationship between chromosome number and morphology of naturally occurring 18 genotypes of Turkish Lolium perenne. The chromosome number for all the studied genotypes was concurrently determined as 2n=14 (x=7). Their karyotypes showed a predominance of metacentric chromosomes. In the karyotype of one genotype, submetacentric chromosomes were dominant. A total of quantitative asymmetric indices were used to evaluate karyological features of the species. A meaningful dendrogram was carried out to assess the karyotype-symmetry conditions and described the karyotype relationships between different cytotypes. Chromosome lengths in a karyotype were between 1.52-2.95 mu m and there was a broad variation in asymmetric index karyotype formula. It may be proposed that the populations of the Turkish perennial ryegrass have quite an important potential relevant to chromosomal variation, and therefore this variation gives essential opportunities to agronomists to develop new cultivars in the future.Öğe Determination of Yield and Quality Characteristics of Alfalfa (Medicago sativa L.) Varieties Grown in Different Locations(MEDWELL ONLINE, 2013) Avci, Mehmet Ali; Ozkose, Abdullah; Tamkoc, AhmetThis research was made in Konya and Polath-Ankara ecological conditions during the years of 2006 and 2007 according to Randomized Blocks Design with three replications The used alfalfa species were Verko, Sunter, Hemedan, Elci and Planet varieties and Kayseri population In the study, the fallowing characteristics were determined plant height (cm), stem thickness (mm), green herbage yield (t ha(-1)), dry matter yield (t ha(-1)), crude protein content (%) and crude protein yield (t ha(-1)) As the means of 2 years and two locations, the genotypes showed the fallowing values 620-682 cm for plant height, 30-32 mm for stem thickness, 838-891 t ha(-1) for green herbage yield, 209-223 t ha(-1) for dry matter yield, 185-194% for crude protein content and 402-422 t ha(-1) for crude protein yield The main goal of alfalfa farming is production of high quality and abundant roughage Kayseri population showed the highest dry matter yield (22 29 kg/da/year) and it was followed by the Verko (22 25 kg/da/year) variety The Verko variety had higher yield than Elci and partially more than Hemedan variety.Öğe Effect of Salinity Stress on Nutrient Composition of Field Pea Genotypes (Pisum sativum. sp. arvense L.)(MEDWELL ONLINE, 2008) Yildinim, Buenyamin; Yasar, Fikret; Terzioglu, Oemer; Tamkoc, Ahmet; Tuerkoezue, DidemThe present study aimed to investigate the effect of different days (0, 7 and 15th days) and salt applications (salty and saltness) on nutrient element compositions of root, shoot and leaf organs of I I field pea genotypes (which are nominate pea) and 2 pea cultivars. For this aim, the data were analyzed using three-way-ANOVA (genotypes, salt application and time). In this study determining, the effects of salt stress on nutrient element compositions of these organs, although the effects of salt application, salt by variety and salt by day interactions on Cu element in root were only found to be non-significant, genotypes, salt application, days and their interactions with 2 and 3 degree for other elements were found to be significant. In root and shoot organs, salt application increased significantly Ca, Mg and Zn amounts, but other minerals decreased compared to control group. The application in leaf increased Ca and Mg, whereas others reduced.Öğe Evaluation of serine carboxypeptidase (PsCP) gene expression in Pisum arvense L. grown under different boron levels(ELSEVIER SCIENCE BV, 2012) Hakki, Erdogan Esref; Tamkoc, Ahmet; Hamurcu, Mehmet; Can, Nimet; Demiral, Tijen; Gezgin, Sait; Ozdemir, Canan[Abstract not Available]Öğe INTER AND INTRA-SPECIFIC VARIATION IN SDS-PAGE OF SEED PROTEINS OF THREE POA L. (POACEAE) SPECIES(PAKISTAN BOTANICAL SOC, 2011) Tamkoc, Ahmet; Arslan, EmineTotal seed storage proteins were analyzed in genotypes belonging to three Poa species collected from Turkey. In this study, SDS-PAGE method was preferred to inter and intraspecifically determine the genetic relationships of Poa species in order to facilitate genotype selection in breeding programs. Individual electrophoregrams of the species obtained with SDS-PAGE can be used as password data for their genetic relationships. Polypeptide patterns ranged from 10 kDa to 128 kDa were calculated according to Nei homology using Bio1D++ computer program. Genetic and morphological differences between the Poa species are discussed. The dendrogram obtained with UPGMA clustering method indicated a low intra-specific genetic diversity while P. pratensis and P. trivialis genotypes stated closer relation as compared to P. angustifolia genotypes. It was concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and genetic relationships of Poa species. Additionally, this method can be useful to determine the correct starting material for plant breeding.Öğe Issr Analysis of Perennial Ryegrass (Lolium Perenne L.) Genotypes and Association Study Between Phenotypes and Genotypes(Elsevier Science Bv, 2012) Kayis, Seyit Ali; Tamkoc, Ahmet; Uygan, Songul; Hakki, Erdogan EsrefBreeders can efficiently use this molecular information in their breeding programs by adding and removing photoperiod insensitive Ppd-D1a alleles to their varieties. In hexaploid wheat, photoperiod insensitive PpdD1a allele in 2D chromosome causes early flowering in short day length and long day length, avoiding stresses associated with high temperature and water deficit in grain filling stages. This study shows that the parents NE01533, Pelsart, Promontory and the 47 lines in F2 generation have the Ppd-D1a allele while Yellowstone and the remaining lines are photoperiod sensitive.Öğe MORPHOLOGICAL AND AGRONOMIC CHARACTERISTICS OF PERENNIAL RYEGRASS (Lolium perenne L.) GENOTYPES(SOC FIELD CROP SCI, 2014) Ozkose, Abdullah; Tamkoc, AhmetMany plants in natural flora including perennial ryegrass loss their genetic variability, suffer from genetic pollution or become extinction. In Turkey, studies on breeding of perennial ryegrass genotype collections are inadequate. This study aimed to determine some plant characteristics of perennial ryegrass plants collected from the natural flora of Ankara. Those genotypes were utilized in order to evaluate for breeding purposes. Seedlings were reproduced in greenhouse after collected seeds from plants were sown in pots. Seedlings were transplanted to the experimental field at 50 x 50 cm intervals in Randomized Complete Block Design with four replications during 2005. Data were obtained from the observations and 568 perennial ryegrasses in 2007 and 2008. The observations and measurement of the two year average values ranged from 2.25 to 6.50 (1=very early, 9=very late) for the spring re-growth time, 2.75 to 7.75 (1=very light green, 9=very dark green) for color, 1.25 to 6.88 (1=very low, 9=very high) for density, 1.0 - 8.0 (1=erect, 9=prostrate) for growth habit, 1.0 - 9.0 (1=very weak, 9=very strong) for tendency to inflorescences, 1.0 to 7.5 (1=very early, 9=very late) for time of inflorescence emergence. In the study, furthermore, measurements varied between 19.35 and 48.05 cm for plant height, 8.31 and 25.54 cm for length of upper internode, 6.76 and 16.28 cm for spike length, 6.44 and 14.57 cm for leaf length, 2.41 and 4.01 mm for leaf width, 1.68 and 4.87 cm(2) for leaf area, 21.23 and 45.52 for leaf shape, 11.63 and 23.75 for spikelet number per spike, 0.031 and 0.151 g spike(-1) for seed yield, 1.23 and 2.38 g for thousand grain weight. The findings showed that there were significant differences among genotypes in terms of investigated traits. The high level of genotypic variability increased the possibility for selection of suitable genotypes. Research results indicated that superior genotypes of perennial ryegrass could be utilized in future breeding programsÖğe PERENNIAL RYEGRASS (LOLIUM PERENNE L.) GENETIC RESOURCES IN THE CENTRAL ANATOLIA FLORA AND ENVIRONMENTAL PRESSURES ON BIODIVERSITY(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2015) Ozkose, Abdullah; Avci, Mehmet Ali; Tamkoc, AhmetThis research was made in 2006-2007 to collect and develop perennial ryegrass genotypes which exist in the natural flora of Turkey. During plant collection, it was observed that perennial ryegrass genotypes were damaged due to decrease of meadows and pasture areas, and the increase of grazing pressure in these areas. Decrease of wetlands, marsh areas and drying of lakes, or decrease of their area depending on precipitation reduction and over-consnmption of water resources negatively influenced perennial ryegrass genotypes in the natural flora. Like many other plants in natural flora, perennial ryegrass genotypes are under great pressure and threat. Therefore, it must be concentrated on activities for improvement of plant varieties and protection of biological diversity of natural ryegrass resources, which has been decreasing day by day.Öğe Study of Genotype x Environment Interaction on Agricultural and Quality in Sainfoin (Onobrychis sativa) Genotypes(MEDWELL ONLINE, 2013) Avci, Mehmet Ali; Ozkose, Abdullah; Tamkoc, AhmetThis research was conducted to determine yield, yield components and quality characteristics of sainfoin genotypes m two locations winch were consisted from Selcuk University, Agricultural Faculty trial field (Konya) and Turkoba Village (Polath/Ankara) according to the Randomized Blocks Design with 4 replications Some traits such as plant height (cm), stem thickness (cm), green herbage yield (t ha(-1)), dry herbage yield (t ha(-1)), crude protein content (%), crude protein yield (kg ha(-1)) and ash content (%) were examined The characteristics of dry herbage yield, crude protein content and crude protein yield winch are known as the most important properties m forages were found as fallowing, 3 275-4 958 t ha(-1), 10 08-13 55% and 355 00-602 25 kg ha(-1), respectively.Öğe Variations in Response to Salt Stress among Field Pea Genotypes (Pisum sativum sp.arvense L.)(MEDWELL ONLINE, 2008) Yildirim, Buenyamin; Yasar, Fikret; Oezpay, Taylan; Tuerkoezue, Didem; Terziodlu, Oemer; Tamkoc, AhmetThis Study examined the responses to salt stress in 11 pea genotypes and two pea cultivars. Pea genotypes were selected from among wild pea and white flowered pea cross-breed varieties from different regions in Anatolia. In general, root fresh weight (fw) shoot fw, leaf fw, leaf number and chlorophyll contents were found to decrease with increases in salinity, whereas root and leaf MDA ratios increased with increases in salinity. Genotypes 1 (10431), 9 (101917) and 10 (1103220) were found to be the most salt-tolerant. Genotypes 6 (110121) and 7 (B-6) were found to be the most sensitive.
