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    GC-MS analysis and antimicrobial activity of alkaloid extract from Genista vuralii
    (TAYLOR & FRANCIS LTD, 2009) Erdemoglu, Nurgun; Ozkan, Semiha; Duran, Ahmet; Tosun, Fatma
    In the present study, the alkaloid composition of the aerial parts of Genista vuralii A. Duran & H. Dural (Fabaceae) was investigated by capillary GC-MS. Ten quinolizidine alkaloids were identified by capillary GC-MS, namely, N-methylcytisine, cytisine, tetrahydrorhombifoline, 17-oxosparteine, 5,6-dehydrolupanine, lupanine, 17-oxolupanine, anagyrine, baptifoline, and 13 alpha-tigloyloxylupanine. Among them, anagyrine (93.04%) was the most abundant alkaloid. Furthermore, antibacterial and antifungal activities of the alkaloid extract of G. vuralii were tested against standard strains of bacteria (Escherichia coli, Pseudomonos aeruginosa, Bacillus subtilis, Staphylococcus aureus) as well as fungi (Candida albicans, Candida krusei). The alkaloid extract of G. vuralii presented good activity against S. aureus, B. subtilis, and C krusei, with minimum inhibitory concentrations (MIC) of 62.5 mu g/mL. The remaining MIC values were found to range between 125 and 500 mu g/mL. To the best of our knowledge, the current work is the first to report the alkaloid profile and antimicrobial activity of G. vuralii L. growing in Turkey.
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    LC-MS quantification of parthenolide and cholinesterase inhibitory potential of selected Tanacetum L. (Emend. Briq.) taxa
    (ELSEVIER, 2015) Orhan, Ilkay Erdogan; Tosun, Fatma; Gulpinar, Ali Rifat; Kartal, Murat; Duran, Ahmet; Mihoglugil, Feyyaz; Akalgan, Demet
    The acetonitrile extracts of various Tanacetum L. (Emend. Briq.) taxa from Turkey as well as parthenolide, a sesquiterpene lactone found in Tanacetum species as active substance were investigated for their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), the key enzymes in pathogenesis of Alzheimer's disease, at 100 mu g mL(-1) using ELISA microplate assay. Most of the extracts displayed a remarkable AChE inhibition where the leaf of Tanacetum argenteum subsp. flabellifolium had the highest inhibition (96.68 +/- 0.35%). The extracts had moderate inhibition toward BChE, among which the stem of Tanacetum argyrophyllum var. argyrophyllum-1 exerted the best inhibition (63.81 +/- 3.64%). However, parthenolide exhibited low inhibition against both of the enzymes. Total flavonoid content of the extracts was determined spectrophotometrically. Parthenolide, a sesquiterpene lactone, was quantified in these taxa by LC-MS and the leaf of T. argenteum subsp. argenteum possessed the richest parthenolide amount (2.261 +/- 0.002%), while most of the species screened were found to contain the required percentage (0.2% minimum) by European Pharmacopeia. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
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    Quantification of genistein and daidzein in two endemic Genista species and their antioxidant activity
    (SERBIAN CHEMICAL SOC, 2011) Orhan, Ilkay Erdogan; Tosun, Fatma; Tamer, Ugur; Duran, Ahmet; Alan, Burcu; Kok, Ali Fuat
    In the current research, the total and free genistein and daidzein contents were determined in two endemic Genista species (G. sandrasica and G. vuralii) by an HPLC method. The highest amount of total genistein and total daidzein was found in G. sandrasica, 0.582 and 0.113 %, respectively, whereas only the free daidzein content of G. sandrasica was higher than that of G. vuralii. The antioxidant activity of the crude methanol and hydrolyzed extracts of these species was evaluated by three in vitro methods; namely DPPH free radical scavenging, ferrous ion-chelating and ferric-reducing antioxidant power (FRAP) tests at 0.25, 0.50, and 1.0 mg ml(-1). The hydrolyzed extracts of both species displayed greater antioxidant activity than the crude methanol extracts in all tests. Total phenol and flavonoid contents in the extracts were determined via the Folin Ciocalteau and AlCl3 reagents, respectively. G. vuralii was richer in terms of total phenol and flavonoid contents compared to G. sandrasica.