Quantification of genistein and daidzein in two endemic Genista species and their antioxidant activity
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SERBIAN CHEMICAL SOC
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In the current research, the total and free genistein and daidzein contents were determined in two endemic Genista species (G. sandrasica and G. vuralii) by an HPLC method. The highest amount of total genistein and total daidzein was found in G. sandrasica, 0.582 and 0.113 %, respectively, whereas only the free daidzein content of G. sandrasica was higher than that of G. vuralii. The antioxidant activity of the crude methanol and hydrolyzed extracts of these species was evaluated by three in vitro methods; namely DPPH free radical scavenging, ferrous ion-chelating and ferric-reducing antioxidant power (FRAP) tests at 0.25, 0.50, and 1.0 mg ml(-1). The hydrolyzed extracts of both species displayed greater antioxidant activity than the crude methanol extracts in all tests. Total phenol and flavonoid contents in the extracts were determined via the Folin Ciocalteau and AlCl3 reagents, respectively. G. vuralii was richer in terms of total phenol and flavonoid contents compared to G. sandrasica.
Açıklama
Anahtar Kelimeler
Genista L., Fabaceae, isoflavones, daidzein, genistein, HPLC, antioxidant
Kaynak
JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
76
Sayı
1
