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    Alchemilla vulgaris agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties
    (ROYAL SOC CHEMISTRY, 2019) Vlaisavljevic, Sanja; Jelaca, Sanja; Zengin, Gökhan; Mimica-Dukic, Neda; Berezni, Sanja; Miljic, Milorad; Stevanovic, Zora Dajic
    The current study was designed to evaluate the phytochemical profile and biological properties (antioxidant, enzyme inhibitory and cytotoxic activity) of methanolic, ethanolic, ethyl-acetate and water extracts of Alchemilla vulgaris from Southeast Serbia (central Balkan) which was traditionally used to alleviate and treat many diseases. Bioactive compounds were characterized by LC-MS/MS technique and biological properties were evaluated using antioxidant, enzyme inhibitory and cytotoxic assays. Twenty-six phenolic compounds were quantified in methanolic, ethanolic, ethyl acetate and water extracts, whereas the highest yields were found in ethyl-acetate (EA) extract (gallic acid, caffeic acid, catchin, quercetin). This extract has also shown the greatest antioxidant, anticancer and inhibitory enzyme activities which were demonstrated for the first time in this study. The obtained results indicated that Alchemilla vulgaris from South Serbia possesses high potential for pharmaceutical applications.
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    Lotus aegaeus (Gris.) Boiss and lberis sempervirens L.: Chemical fingerprints, antioxidant potential, and inhibition activities and docking on key enzymes linked to global health problems
    (ELSEVIER SCIENCE BV, 2018) Mahomoodally, Mohamad Fawzi; Vlaisavljevic, Sanja; Berezni, Sanja; Abdallah, Hassan Hadi; Zengin, Gökhan; Atanasov, Atanas G.; Mollica, Adriano
    Natural products are gaining much momentum worldwide because they have a broad spectrum of biological effects and present excellent opportunities for bioproducts development. With this fact, this study has been designed to investigate for the first time the effects of the ethyl acetate (EA), methanolic (MeOH), and water (W) extracts of Lotus aegaeus (La) and Iberis sempervirens (Is) on key enzymes as well as to quantify antioxidant capacities. The total phenolic and flavonoid content were determined using colorimetric methods and the individual phenolic compounds were assessed by LC-MS/MS analysis. Quercetin hexoside was the dominant compound in L. aegaeus, particularly in methanol extract, while the ethyl acetate and methanol extracts of I. sempervirens were characterized by the presence scopoletin and hinic acid. The antioxidant abilities of the investigated extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating. All the extracts showed strong antioxidant abilities. For both species, the ethyl acetate extracts were more potent against cholinesterases and alpha-amylase, while the methanol extracts were most active against a-glucosidase. Only the water extract, followed by methanol of L sempervirens exhibited notable inhibitory activity against tyrosinase. In addition, the dominant compounds were docked against tyrosinase and a-glucosidase to investigate their predicted binding affinity and interactions with the active site. In conclusion, L. aegaeus and I. sempervirens showed potent biological attributes, which advocates for further studies to explore their potential use as phytopharmaceuticals.