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Öğe CAPRINE ARTHRITIS ENCEPHALITIS VIRUS INFECTION IN KIDS WITH ARTHRITIS(INDIAN VETERINARY JOURNAL, 2008) Acar, A.; Yavru, S.; Kale, M.; Bulut, O.; Avci, O.; Pehlivanoglu, F.Infection by caprine arthritis encephalitis virus (CAEV) is a worldwide cause of slowly progressing inflammatory disease of joints, mammary gland, lung and less commonly brain of mature goats. Chronic, proliferative arthritis in mature goats is the predominant clinical disease manifestation of CAE (Cheevers et al, 1988). The present study records a clinical presentation of CAE, in kids I to 3 months of age, with arthritis.Öğe Comparison of the Detection of Influenza A and B Viruses by Different Methods(SAGE PUBLICATIONS LTD, 2012) Ozdemir, M.; Yavru, S.; Baysal, B.OBJECTIVE: To investigate the detection of influenza viruses by three different methods. METHODS: Nasopharyngeal swabs were collected from patients with influenza symptoms and examined for influenza A and B viruses using a rapid antigen test, a multiplex polymerase chain reaction (PCR) test and a shell vial cell culture test. RESULTS: Using the shell vial cell culture test, the rapid antigen test and the multiplex PCR test in 130 patients, 31 (23.8%), 24 (18.5%) and 24 (18.5%) samples, respectively, were positive for influenza A and 10 (7.7%), nine (6.9%) and four (3.1%) samples, respectively, were positive for CHAIN REACTION TEST; influenza B. Compared with the shell vial test, the sensitivity, specificity, and positive and negative predictive values of the rapid antigen test were 77.4%, 93.3%, 80.0% and 93.1%, respectively, for influenza A, and 90.0%, 95.8%, 64.2% and 99.1%, respectively, for influenza B. The corresponding values for the multiplex PCR test were 77.4%, 95.9%, 85.7% and 93.1%, respectively, for influenza A, and 40.0%, 97.5%, 57.1% and 95.1%, respectively, for influenza B. CONCLUSIONS: The multiplex PCR test and the rapid antigen test are both effective in the detection of influenza A and B viruses.Öğe Determination of Culicoides species (Diptera: Ceratopogonidae) as suspect vectors of Epizootic Haemorrhagic Disease and Bluetongue viruses in southern and western Anatolia by RT-PCR(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2012) Dik, B.; Yavru, S.; Uslu, U.; Yapici, O.; Esin, E.This study was performed to detect genomes of the Epizootic Haemorrhagic Disease Virus (EHDV) and Bluetongue virus (BTV) in Culicoides species present in various areas from Anatolia. Culicoides samples were caught using Onderstepoort-type light traps in 18 districts from 7 Provinces (Antalya, Mugla. Aydin, Izmir, Manisa, Balikesir and Canakkale) and the presence of viral genomes was investigated by RT-PCR using specific primer pairs (EHDV/S3 and BTV/S7, respectively). Out of the 13 Culicoides species collected. C. imicola complex was predominant following by Culicoides sp. C. nubeculosus, C schultzei, C circumscriptus and C. longipennis. The BTV genome was not evidenced in any diptera species whereas tie presence of EHDV was detected in 9 species (C. imicola, C. circumscriptus, C. festivipennis, C. gejgelensis, C. longipennis, C. nubeculosus, C. obsoletus, C. pulicaris and Culicoides sp.) depending for the localisation of the collecting centers. The viral genome was evidenced in C. pulicaris and C. imicola complexes. in C. festivipennis, C. gejgelensis, C. longipennis and Culicoides sp. caught in Mugla, in C. imicola, C. longipennis and C. tuberculosus caught in Izmir and in C. obsoletus and C. circumscriptus collected in the Canakkale province. These results show that all of the Culicoides species, from which the viral genome was detected by RT-PCR for the first time in Turkey, were potential EHDV vectors.Öğe The effect of infection with bovine viral diarrhea virus on the fertility of cows and heifers(DE GRUYTER POLAND SP ZOO, 2006) Kale, M.; Ata, A.; Yavru, S.; Yapkic, O.; Bulut, O.; Gulay, M. S.In the current study, blood samples from 319 cows and heifers were studied. Antibodies against BVDV infections in serum samples and BVDV antigens in leukocytes were present in 203 (148 cows and 55 heifers) and 56 (28 cows and 28 heifers) samples, respectively. Although no significant difference was detected between seropositive [BVDV (Ag-/Ab+)] and uninfected [BVDV (Ag-/Ab-)] cows and heifers at the time of the first insemination (FIT), first service age (FSA) or conception rate (CR) (p > 0.05), the difference in age of seropositive and infection free cows differed significantly (p < 0.05). Furthermore, differences in FIT, FSA or age of seropositive and uninfected pregnant cows and heifers did not differ significantly (p > 0.05). Differences between persistently infected [BVDV (Ag +/Ab -)] and uninfected cows for FIT, CR and age were statistically different (p < 0.001). On the other hand, the difference between persistently infected and uninfected heifers for FSA or CR did not differ significantly (p > 0.05). Even though, FSA of pregnant persistently infected or uninfected heifers was not different (p > 0.05), there was a difference between the age and FIT of persistently infected and uninfected cows (p < 0.001). However, pregnancy was not detected in any of the persistently infected cows. In conclusion, seropositivity for BVDV did not affect fertility of cows or heifers. Although, differences in fertility between persistently infected and uninfected pregnant cows and between persistently infected and uninfected non-pregnant cows were present, fertility of heifers was not affected by BVDV persistency.Öğe The effect of subclinical bovine herpesvirlis 1 infection on fertility of cows and heifers(DE GRUYTER POLAND SP ZOO, 2006) Ata, A.; Kale, M.; Yavru, S.; Bulut, O.; Buyukyoruk, U.The aim of this study was to determine the serological prevalence of bovine herpesvirus 1 (BHV-1), and describe whether natural subclinical infections will lead to fertility losses in dairy cows and heifers in Burdur, Turkey. BHV-1 prevalence was 11.94% (201/24) and 14.60% (89/13) in cows and heifers, respectively. Conception rate (CR) was higher in BHV-1 serologically negative cows (38.98%) than BHV-1 positive cows (33.33%) but the difference was statistically insignificant (P > 0.05). However, CR was higher (P > 0.05) in BHV-1 serologically positive heifers (84.61%) than BHV-1 negative heifers (56.57%). Average open days period (OD) of BHV-1 serologically positive cows (99.3 +/- 16 d) was different from BHV-1 negative cows (82.0 +/- 3.8 d) (P < 0.05). Average for the first service (FSA) of BHV-1 serologically positive heifers (18.01 +/- 1.1 mo) did not differ from BHV-1 negative heifers (18.0 +/- 0.3 mo) (P > 0. 05). Results of the current study showed that BHV-1 serologically positive cows have required for successful conception about 17 days more than negative cows.Öğe Effects of subclinical bovine leukemia virus infection on some production parameters in a dairy farm in southern Turkey(AOSIS OPEN JOURNALS, 2007) Kale, M.; Bulut, O.; Yapkic, O.; Gulay, M. S.; Pehlivanoglu, F.; Ata, A.; Yavru, S.Some production parameters of seropositive cows (age, first calving age, 305 day mature equivalent last milk yield production, lifetime mature equivalent milk yield production, lifetime total milk production, lifetime total milking period, lifetime monthly milk production, lifetime daily milk production, lifetime total days of milking, number of inseminations per pregnancy (for last pregnancy), number of calves and calving interval (for last pregnancy)) were analysed in the current study. The study population was clinically healthy Holstein cows from a commercial dairy herd in southern Turkey. Of 109 animals, 65 cows were seropositive by ELISA and the prevalence of bovine leukemia virus (BLV) infection was 59.6%. The prevalence of seropositive cows in 2nd (62.8%), 3rd (64.7%), 4th (61.5%), and 5th (66.6%) lactations was slightly higher than that of cows in 1st (52.6%) lactations. No statistical differences were observed between BLV seronegative and seropositive cows for production and reproduction parameters analysed in this study (P > 0.05).Öğe An investigation of equine infectious anaemia infection in the Central Anatolia region of Turkey(SOUTH AFRICAN VET ASSOC, 2007) Yapkic, O.; Yavru, S.; Kale, M.; Bulut, O.; Simsek, A.; Sahna, K. C.In this study, 162 horses, 80 donkeys and 51 mule serum samples were collected in Konya city. Additionally, 64 horse serum samples from Ankara and 49 samples from Kayseri city were included in the study. A total of 406 serum samples were examined by agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) for antibody to equine infectious anaemia virus (EIAV) and no positive result was detected.Öğe Isolation of Bovine Herpesvirus Type 1 From Bovine Semen in Turkey(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2001) Yavru, S.; Öztürk, F.; Şimşek, A.; Yapkıç, O.; Yıldız, C.In this study, semen samples from sixty bulls were used for virus isolation into sensitive cell culture. Five viruses were isolated by inoculation of 60 semen samples into the cell cultures. The isolated viruses were identified as BHV-1 according to results of cross microneutralization test by using hyperimmun sera from rabbits. Obtained data showed that BHV-1 may exist in the semen, and sensitive cattle may be infected by virus via insemination.Öğe Isolation of epizootic hemorrhagic disease virus from sheep in western Turkey(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2014) Yavru, S.; Erol, N.; Avci, O.; Esin, E.; Pasa, S.Epizootic hemorrhagic disease (EHD) is a systemic viral infection of ruminants, especially deer, characterized by systemic blood circulation disorders and death. Data on the prevalence, clinical and pathological characteristics, and virulence of the infection in sheep are very limited in the literature. In this study, virological details of epizootic hemorrhagic disease virus (EHDV) were investigated in sheep with clinical symptoms such as high fever, edema of the head, particularly under the chin, and lesions in the mouth and nose in the Aydin province of western Turkey. The blood serum samples taken from animals with clinical symptoms were tested for the presence of specific antibodies against bluetongue virus (BTV) and EHDV using competitive ELISA (cELISA) and agar gel precipitation test (AGPT), respectively. In addition, Bluetongue Virus Antigen Capture ELISA (BTACE) kit was used to test for BTV antigens. Supernatants collected from the cell cultures with cytopathogenic effect were subjected to BTACE to test for the presence of BTV antigens. One step RT-PCR was used to examine samples for the presence of BTV and EHDV genomes. No antibodies against BTV and EHDV and no BTV antigens were detected. However, a band of 533 base pairs was detected by RT-PCR in two of the samples, confirming the presence of the EHDV genome. These findings suggest that EHDV infection could be present in sheep in the region and may be the cause of subclinical or atypical symptoms that result in difficulties in the differential diagnosis of other viral infections.Öğe Serological and virological investigation of bovine viral diarrhea virus (BVDV) infection in cattle with abortion problem(WILEY-BLACKWELL, 2012) Bulut, O.; Avci, O.; Yapici, O.; Yavru, S.; Simsek, A.[Abstract not Available]Öğe A serological survey of bovine herpesvirus-1 infectiom in beef herds in Turkey(INDIAN VETERINARY JOURNAL, 2007) Duman, R.; Yavru, S.; Bulut, O.; Kale, M.Infectious bovine rhinotracheitis (IBR) is caused by the bovine herpesvirus type I is an enzootic disease on the. B list of the Office International des Epizooties (OIE). The serum neutralization test, (SNT) is routinely used for serodiagnosis of IBR virus infection in cattle (OIE, 2000). Serological testing of the animals for BHV-1 infection is important for herd health and economical aspect since BHV-1 latent infected animals can cause an outbreak after reinfection. The purpose of this study was to determine antibodies to BHV-1 in beef cattle sera from central and southern regions of Turkey.Öğe Treatment of Bovine papillomavirus-induced teat warts in a cow by using Podophyllin magistral formula and autologous vaccine applications together(AGRICULTURAL RESEARCH COMMUNICATION CENTRE, 2019) Kale, M.; Saltik, H. S.; Hasircioglu, S.; Yildirim, Y.; Yavru, S.; Mamak, N.; Atli, K.In this study, a 32-month-old Holstein cow that had a calving and common Bovine papillomavirus (BPV)-induced wart on its teats was treated. For this purpose, BPV antigen presence in blood serum sample from the animal was studied by ELISA and was revealed as positive. Magistral drug containing podophyllin active substance was applied topically on teat warts of the animal Applications were performed for all teat warts once in three days during 27 days. Eight hours after applying the drug, teats were washed with sterile water. Warts on teat were proved to have regressed and dried out on the day 27th. The same animal was given papilloma autologous vaccine prepared from warts coming off from the teat on the day 28th. As a result of the vaccination, teats showed full recovery from warts. Finally, it is recommended that using podophyllin formulations topical and autologous vaccine applications together in treating teat warts caused by BPV.