Determination of Culicoides species (Diptera: Ceratopogonidae) as suspect vectors of Epizootic Haemorrhagic Disease and Bluetongue viruses in southern and western Anatolia by RT-PCR
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ECOLE NATIONALE VETERINAIRE TOULOUSE
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study was performed to detect genomes of the Epizootic Haemorrhagic Disease Virus (EHDV) and Bluetongue virus (BTV) in Culicoides species present in various areas from Anatolia. Culicoides samples were caught using Onderstepoort-type light traps in 18 districts from 7 Provinces (Antalya, Mugla. Aydin, Izmir, Manisa, Balikesir and Canakkale) and the presence of viral genomes was investigated by RT-PCR using specific primer pairs (EHDV/S3 and BTV/S7, respectively). Out of the 13 Culicoides species collected. C. imicola complex was predominant following by Culicoides sp. C. nubeculosus, C schultzei, C circumscriptus and C. longipennis. The BTV genome was not evidenced in any diptera species whereas tie presence of EHDV was detected in 9 species (C. imicola, C. circumscriptus, C. festivipennis, C. gejgelensis, C. longipennis, C. nubeculosus, C. obsoletus, C. pulicaris and Culicoides sp.) depending for the localisation of the collecting centers. The viral genome was evidenced in C. pulicaris and C. imicola complexes. in C. festivipennis, C. gejgelensis, C. longipennis and Culicoides sp. caught in Mugla, in C. imicola, C. longipennis and C. tuberculosus caught in Izmir and in C. obsoletus and C. circumscriptus collected in the Canakkale province. These results show that all of the Culicoides species, from which the viral genome was detected by RT-PCR for the first time in Turkey, were potential EHDV vectors.
Açıklama
Anahtar Kelimeler
Epizootic haemorrhagic disease virus, bluetongue virus, Culicoides, vector, viral genome, RT-PCR, southern and western Anatolia, Turkey
Kaynak
REVUE DE MEDECINE VETERINAIRE
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
163
Sayı
11
