Determination of Culicoides species (Diptera: Ceratopogonidae) as suspect vectors of Epizootic Haemorrhagic Disease and Bluetongue viruses in southern and western Anatolia by RT-PCR

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dc.contributor.authorDik, B.
dc.contributor.authorYavru, S.
dc.contributor.authorUslu, U.
dc.contributor.authorYapici, O.
dc.contributor.authorEsin, E.
dc.date.accessioned2020-03-26T18:24:42Z
dc.date.available2020-03-26T18:24:42Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study was performed to detect genomes of the Epizootic Haemorrhagic Disease Virus (EHDV) and Bluetongue virus (BTV) in Culicoides species present in various areas from Anatolia. Culicoides samples were caught using Onderstepoort-type light traps in 18 districts from 7 Provinces (Antalya, Mugla. Aydin, Izmir, Manisa, Balikesir and Canakkale) and the presence of viral genomes was investigated by RT-PCR using specific primer pairs (EHDV/S3 and BTV/S7, respectively). Out of the 13 Culicoides species collected. C. imicola complex was predominant following by Culicoides sp. C. nubeculosus, C schultzei, C circumscriptus and C. longipennis. The BTV genome was not evidenced in any diptera species whereas tie presence of EHDV was detected in 9 species (C. imicola, C. circumscriptus, C. festivipennis, C. gejgelensis, C. longipennis, C. nubeculosus, C. obsoletus, C. pulicaris and Culicoides sp.) depending for the localisation of the collecting centers. The viral genome was evidenced in C. pulicaris and C. imicola complexes. in C. festivipennis, C. gejgelensis, C. longipennis and Culicoides sp. caught in Mugla, in C. imicola, C. longipennis and C. tuberculosus caught in Izmir and in C. obsoletus and C. circumscriptus collected in the Canakkale province. These results show that all of the Culicoides species, from which the viral genome was detected by RT-PCR for the first time in Turkey, were potential EHDV vectors.en_US
dc.description.sponsorshipScientific Research Projects Unit (BAP) of Selcuk UniversitySelcuk University [08401068]en_US
dc.description.sponsorshipThis study was funded by the Scientific Research Projects Unit (BAP) of Selcuk University (BAP Project No: 08401068). We would like to thank the official veterinarians who kindly assisted us in provinces and districts We would also like to thank Geomatic Engineer Dr. Ilkay Bugdayci for preparing the map presented in the paper.en_US
dc.identifier.endpage510en_US
dc.identifier.issn0035-1555en_US
dc.identifier.issn2258-0646en_US
dc.identifier.issue11en_US
dc.identifier.startpage505en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27891
dc.identifier.volume163en_US
dc.identifier.wosWOS:000312058100002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherECOLE NATIONALE VETERINAIRE TOULOUSEen_US
dc.relation.ispartofREVUE DE MEDECINE VETERINAIREen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEpizootic haemorrhagic disease virusen_US
dc.subjectbluetongue virusen_US
dc.subjectCulicoidesen_US
dc.subjectvectoren_US
dc.subjectviral genomeen_US
dc.subjectRT-PCRen_US
dc.subjectsouthern and western Anatoliaen_US
dc.subjectTurkeyen_US
dc.titleDetermination of Culicoides species (Diptera: Ceratopogonidae) as suspect vectors of Epizootic Haemorrhagic Disease and Bluetongue viruses in southern and western Anatolia by RT-PCRen_US
dc.typeArticleen_US

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