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    BIOCONTROL OF BOTRYTIS CINEREA ON STRAWBERRY FRUIT BY PLANT GROWTH PROMOTING BACTERIA
    (PAKISTIAN AGRICULTURAL SCIENTISTS FORUM, 2011) Donmez, M. F.; Esitken, A.; Yildiz, H.; Ercisli, S.
    In the present study, a total of 186 bacterial strains isolated from various soil sources and plant species from Eastern Anatolia region in Turkey were evaluated for their ability to suppress gray mold (Botrytis cinerea Pers. ex Fr.) occurred on strawberry cv. Fern. Among 186 bacterial strains, 36 were found effective to inhibit of development B. cinerea under in vitro conditions, and thirteen of them which have greater inhibition zone were selected as biocontrol agent. These antagonistic strains were identified as Bacillus lentimorbus, B. megaterium, B. pumilis, B. subtilis, Enterobacter intermedius, Kurthia sibirica, Paenibacillus polymyxa and Pantoea agglomerans. The inhibition zones among bacteria against B. cinerea were found between 0.50 (Bacillus C6, Brevibacterium MFD-47 and Pantoea MFD-232) and 3.75 cm (Enterobacter MFD-81) in vitro. The strawberry fruits were inoculated with B. cinerea in laboratory trials and Bacillus MFDU-2 (14.41 mm) was found more effective to prevent mycelial development on strawberry fruits in comparison to the control (19.20 mm). In terms of conidia germination on strawberry fruits, the lowest disease incidence was observed in MFD-45 treatment (20.8%), while disease incidence rate for the control was 79.2%. It was shown that antagonistic bacterial strains inhibited B. cinerea and that they have a potential use in sustainable strawberry production.
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    Possible neoplastic effects of acrylamide on rat exocrine pancreas
    (TAYLOR & FRANCIS LTD, 2013) Yener, Y.; Kalipci, E.; Oztas, H.; Aydin, A. D.; Yildiz, H.
    We investigated whether the acrylamide formed during cooking carbohydrate-rich foods at high temperatures causes neoplastic changes in rat pancreas. Azaserine, which is an amino acid derivative that has the ability to initiate neoplastic changes in rat pancreas, was injected into 14-day-old male rats once a week for three weeks. Acrylamide was given to both azaserine-injected and non-injected rats at doses of 5 and 10 mg/kg/day in drinking water for 16 weeks after which tissue slides were prepared from the pancreata. Pancreas weights and body weights of rats treated with azaserine and acrylamide together increased significantly compared to the other groups. Moreover, the size, average diameter and volume of atypical acinar cell foci that developed in the pancreata of rats treated with azaserine and acrylamide together increased significantly compared to rats treated with either azaserine or acrylamide alone and control groups. Atypical acinar cell adenoma or adenocarcinoma was not observed in the pancreata of rats in any group.
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    Short aggrecan gene repetitive alleles associated with lumbar degenerative disc disease in Turkish patients
    (FUNPEC-EDITORA, 2011) Eser, O.; Eser, B.; Cosar, M.; Erdogan, M. O.; Aslan, A.; Yildiz, H.; Solak, M.
    We investigated a possible association between aggrecan gene polymorphism and lumbar degenerative disc disease in Turkish patients. One hundred 20-30-year-old patients with or without low back pain were selected for the study. Lumbar magnetic resonance imaging was performed on all patients. The patient group had low back pain clinically and degenerative disc disease radiographically. The control group included patients with and without low back pain: all were negative radiographically for degenerative disc disease. Genomic DNA was extracted from all participants. A PCR assay were used to evaluate variable number of tandem repeat polymorphism of aggrecan gene alleles to determine if there was any correlation with degenerative disc disease. Significant associations were found between short repeated alleles of the aggrecan gene and severe disc degeneration. A significant association was also found between short repeated alleles of the aggrecan gene and multilevel disc herniation as well as extrusion and sequestration types of disc herniation. In Turkish population, short repeated alleles of the aggrecan gene are associated with increased disc degeneration and disc herniation.