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    Design of a Probe Based on Poly(glycidyl methacrylate-co-vinylferrocene)-Coated Pt Electrode for Electrochemical Detection of PTEN Gene in PCR Amplified Samples from Prostate Tissues
    (WILEY, 2014) Bas, Salih Zeki; Maltas, Esra; Sennik, Busra; Yilmaz, Faruk; Vural, Hasibe Cingilli
    In this study, a new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH2-linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2-linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10-100 g mL(-1)) with a detection limit (4.7 g mL(-1)) and a good selectivity of the NH2-linked DNA probe toward target DNA sequence. (c) 2014 Wiley Periodicals, Inc.
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    Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase
    (ELSEVIER SCIENCE BV, 2014) Bas, Salih Zeki; Maltas, Esra; Sennik, Busra; Yilmaz, Faruk
    Xanthine oxidase (XO) was successfully immobilized by covalent attachment on poly(glycidyl methacrylate-co-vinylferrocene) (P(GMA-co-VFc)), a redox copolymer containing pendant epoxy and ferrocene moieties, for the evaluation of both the biosensing properties and the effect of the interaction of ketoconazole (Ktc) with the immobilized XO. The binding interaction between Ktc, a drug used to treat fungal infections, and the immobilized XO on P(GMA-co-VFc) was also studied by fluorescence spectroscopy technique. The binding capacity of the drug was determined using a calibration curve equation that was drawn at excitation wavelength of 300 nm using fluorescence spectroscopy. The interaction ability was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and native-PAGE analysis. The enzyme electrode exhibited a linear range from 2.7 x 10(-3) to 0.55 mM with a sensitivity of 19.42 mu A mM(-1) cm(-2) and a detection limit of 8 x 10(-4) mM for the detection of xanthine. The activation energy (E-a) and the apparent Michaelis-Menten constant (K-mapp) values were found to be 12.30 kJ mol(-1) and 0.38 mM, respectively. (C) 2013 Elsevier B.V. All rights reserved.