Design of a Probe Based on Poly(glycidyl methacrylate-co-vinylferrocene)-Coated Pt Electrode for Electrochemical Detection of PTEN Gene in PCR Amplified Samples from Prostate Tissues
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, a new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH2-linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2-linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10-100 g mL(-1)) with a detection limit (4.7 g mL(-1)) and a good selectivity of the NH2-linked DNA probe toward target DNA sequence. (c) 2014 Wiley Periodicals, Inc.
Açıklama
Anahtar Kelimeler
biomaterials, nucleic acids, sensors and actuators
Kaynak
JOURNAL OF APPLIED POLYMER SCIENCE
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
131
Sayı
16
