Design of a Probe Based on Poly(glycidyl methacrylate-co-vinylferrocene)-Coated Pt Electrode for Electrochemical Detection of PTEN Gene in PCR Amplified Samples from Prostate Tissues

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dc.contributor.authorBas, Salih Zeki
dc.contributor.authorMaltas, Esra
dc.contributor.authorSennik, Busra
dc.contributor.authorYilmaz, Faruk
dc.contributor.authorVural, Hasibe Cingilli
dc.date.accessioned2020-03-26T18:49:58Z
dc.date.available2020-03-26T18:49:58Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, a new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH2-linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2-linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10-100 g mL(-1)) with a detection limit (4.7 g mL(-1)) and a good selectivity of the NH2-linked DNA probe toward target DNA sequence. (c) 2014 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipScientific Research Projects of Selcuk UniversitySelcuk Universityen_US
dc.description.sponsorshipThe authors are grateful for the financial support by the Scientific Research Projects of Selcuk University.en_US
dc.identifier.doi10.1002/app.40638en_US
dc.identifier.issn0021-8995en_US
dc.identifier.issn1097-4628en_US
dc.identifier.issue16en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1002/app.40638
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30721
dc.identifier.volume131en_US
dc.identifier.wosWOS:000336453600042en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofJOURNAL OF APPLIED POLYMER SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectbiomaterialsen_US
dc.subjectnucleic acidsen_US
dc.subjectsensors and actuatorsen_US
dc.titleDesign of a Probe Based on Poly(glycidyl methacrylate-co-vinylferrocene)-Coated Pt Electrode for Electrochemical Detection of PTEN Gene in PCR Amplified Samples from Prostate Tissuesen_US
dc.typeArticleen_US

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